Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Effects of Houttuynia cordata Extracts of Different Aerial Parts on Antioxidants and Anti-inflammatory

  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • Received : 2018.05.03
  • Accepted : 2018.06.25
  • Published : 2018.06.30
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Abstract

The current study was carried out to determine the effects of the leaf and root of Houttuynia cordata Thunb on antioxidant and anti-inflammatory. Total polyphenol contents of leaf and root ethanol extracts were found to be 59.32 and 12.07 mg/g, respectively. Also, total flavonoid contents of leaf and root ethanol extracts were found to be 10.85 and 8.55 mg/g, respectively. The $RC_{50}$ values of DPPH radical scavenging of leaf and root ethanol extracts were 23.51 and $154.72{\mu}g/mL$, respectively. The $RC_{50}$ values of ABTS radical scavenging of leaf and root ethanol extracts were 35.42 and $233.89{\mu}g/mL$, respectively. The antioxidant activities in leaf ethanol extracts were higher in root. Also, to confirm anti-inflammatory activity of ethanol extract, we treat leaf and root of Houttuynia cordata Thunb extract on BV-2 cell with LPS. The NO inhibition effects in of Houttuynia cordata Thunb leaf ethanol extracts showed higher values compared with the root ethanol extracts. These results indicate that Houttuynia cordata Thunb ethanol extracts may play a positive role in antioxidant and anti-inflammatory.

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References

  1. Aischer RG, Hess JL. Antioxidants in higher plants. CRC Press, Boca Raton. 1993. 1-17.
  2. Bouayed J, Bohn T. Exogenous antioxidants-Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative Medicine and Cellular Longevity. 2010. 3: 228-237. https://doi.org/10.4161/oxim.3.4.12858
  3. Chen L, Hu JU, Wang SQ. The role of antioxdants in photoprotection: A critical review. Journal of the American Academy of Dermatology. 2012. 67: 1013-1024. https://doi.org/10.1016/j.jaad.2012.02.009
  4. Chun EY. Partial purification of Houttuynia cordata Thunb extract and characterization of its immunological activities in human. MS Thesis, Seoul National University. 1997.
  5. Decker EA, Crum AD, Calvert JT. Differences in the antioxidant mechanism of carmosine in the prescence of copper and iron. Journal Of Agricultural And Food Chemistry. 1992. 40: 756-759. https://doi.org/10.1021/jf00017a009
  6. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. The Journal of Biological Chemistry. 1912. 12: 239-249.
  7. Gerriten ME. Flavonoids inhibit cytokine-induced endothelial cell adhesion protein gene expression. American Journal of Pathology. 1995. 147: 278-292.
  8. Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell signal. 2001. 13: 85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  9. Huang MT, Ho CT, Lee CY. Phenolic compounds in food. In phenolic compounds in food and their effects on health II. New York: Maple Press. 1992. 2-7.
  10. Hong ND, Kim NJ. Quality control of herbal medicines. Shinil Books, Seoul, Korea. 2004. 476-477.
  11. Kim B, Kim JI, Kim HR, Byun DS. Anti-inflammatory effect of an ethyl acetate fraction from Myagropsis yendoi on lipopolysaccharides-stimulated RAW 264.7 cells. Korean Journal of Fisheries and Aquatic Sciences. 2014. 47: 527-536. https://doi.org/10.5657/KFAS.2014.0527
  12. Kim J, Ryu HS, Shin JH Kim HS. In vitro and ex vivo supplementation of Houttuynia cordata extract and immunomodulating effect in mice. Journal of the Korean Society of Food Science and Nutrition. 2005. 34: 167-175. https://doi.org/10.3746/jkfn.2005.34.2.167
  13. Kwun JA. About Houttuynia cordata Thunb. Korean Oriental Drug. 1998. 2: 218-221.
  14. Nathan C. Nitric oxide as a secretory product of mammalian cells. The FASEB Journal. 1992. 6: 3051-3064. https://doi.org/10.1096/fasebj.6.12.1381691
  15. Nieva, Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 2000. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  16. Park SH, Lim HY, Han JH. A study of medicinal herbs for func0 tional food application (I) Nutritional composition and scopolectin analysis of Artemisia capilaries. The East Asian Society Of Dietary Life. 2003. 13: 552-560.
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Seo DW, Yi YJ, Lee MS, Yun BS, Lee SM. Differential modulation of lipopolysaccharide nduced inflammatory cytokine production by and antioxidant activity of fomentariol in RAW264.7 cells. Mycobiology. 2015. 43: 450-457. https://doi.org/10.5941/MYCO.2015.43.4.450
  19. Suolinna EM, Buchsbaum RN, Racker E. The effect of flavonoids on aerobic glycolysis and growth of tumor cells. Cancer Research. 1975. 35: 1865-1872.
  20. Tanaka H, Dinenno FA, Monahan KD, Clevenger CM, DeSouza CA, Seals DR. Aging, habitual exercise, and dynamic arterial compliance. Circulation. 2000. 102: 1270-1275. https://doi.org/10.1161/01.CIR.102.11.1270
  21. Wiseman H. Dietary influences on membrane function: impotent in protection against oxidative damage and disease. The Journal of Nutritional Biochemistry. 1996. 7: 2-15 https://doi.org/10.1016/0955-2863(95)00152-2