• Title/Summary/Keyword: 6MV

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Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo;Ryu, Ki-Hyun;Kim, Hyun-Ran;Kim, Chung-Sun;Lee, Dong-Woo;Kim, Jeong-Soo;Park, Min-Hye;Noh, Young-Mi;Choi, Sun-Hee;Han, Dong-Hyun;Lee, Chang-Hoo
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.259-265
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    • 2002
  • Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

Studies on the Anodic Oxidation Behavior of Methanol and L-Ascorbic Acid by Using Glassy Carbon Electrodes Modified with Inorganic-Metal Polymeric Films (무기 금속 고분자 막을 도포시킨 유리질 탄소전극을 이용한 메탄올과 L-ascorbic acid의 양극 산화 거동에 관한 연구)

  • Yoo, Kwang-Sik;Woo, Sang-Beom
    • Analytical Science and Technology
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    • v.11 no.5
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    • pp.347-352
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    • 1998
  • A study was carried out on the elelctrochemical characteristics of chemically modified electrodes (CMEs) by cyclic voltammetry. Fabrication of CMEs was made by coating with mixed valence (mv) inorganic-metal polymeric films on the glassy carbon electrode surface by potential cycling. Anodic oxidation behavior of methanol and L-ascorbic acid was studied by using CMEs working electrode. Deposition of films such as mv ruthenium oxo/ruthenium cyanide film (mv Ru-O/CN-Ru), mv ruthenium oxo/ferrocyanide film (mv Ru-O/$Fe(CN)_6$), and mv ruthenium oxo/ruthenium cyanide/Rhodium film (mv Ru-O/CN-Ru/Rh) was obtained to coat by scan rate of 50 mV/sec within the specified potential range (-0.5V ~ +1.2V). Film thickness was controlled by the repeat of the potential cycling. Anodic oxidation behavior of methanol was as follow. Calibration graph by using mv Ru-O/CN-Ru film showed linearly from 10 mM to 80 mM MeOH with slope factor of $-7.552{\mu}A/cm^2$. Although slope factor by using mv Ru-O/$Fe(CN)_6$ film was $-5.13{\mu}A/cm^2$, yet linear range of calibration graph could be extended from 10 mM to 100 mM MeOH. Anodic oxidation behavior of L-ascorbic acid was studied by mv Ru-O/CN-Ru film on the glassy carbon electrode and the glassy carbon electrode with Rh film, Glassy carbon electrode modified with Ru polymeric film was showed better sensitivity than the Rh-glassy carbon modified electrode (mv Ru-O/CN-Ru/Rh). Calibration graph was linear from 0.1 mM to 5 mM L-ascorbic acid by using glassy carbon electrode modified with Ru polymeric film. Solpe factor and relative coefficient are $-84.78{\mu}A/mM$ and 0.998, respectively.

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SPECTRAL DUALITIES OF MV-ALGEBRAS

  • Choe, Tae-Ho;Kim, Eun-Sup;Park, Young-Soo
    • Journal of the Korean Mathematical Society
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    • v.42 no.6
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    • pp.1111-1120
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    • 2005
  • Hong and Nel in [8] obtained a number of spectral dualities between a cartesian closed topological category X and a category of algebras of suitable type in X in accordance with the original formalism of Porst and Wischnewsky[12]. In this paper, there arises a dual adjointness S $\vdash$ C between the category X = Lim of limit spaces and that A of MV-algebras in X. We firstly show that the spectral duality: $S(A)^{op}{\simeq}C(X^{op})$ holds for the dualizing object K = I = [0,1] or K = 2 = {0, 1}. Secondly, we study a duality between the category of Tychonoff spaces and the category of semi-simple MV-algebras. Furthermore, it is shown that for any $X\;\in\;Lim\;(X\;{\neq}\;{\emptyset})\;C(X,\;I)$ is densely embedded into a cube $I^/H/$, where H is a set.

Dosimetric Characteristics of Dual Photon Energy Using Independent Collimator Jaws (고에너지 선형가속기의 Independent Collimator를 이용한 비대칭 방사선 조사시 방사선량 결정에 미치는 요인에 관한 연구)

  • Kim Jeung-kee;Choi Young-Min;Lee Hyung-Sik;Hur Won-Joo
    • Radiation Oncology Journal
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    • v.14 no.3
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    • pp.237-244
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    • 1996
  • Purpose : The accurate dosimetry of independent collimator equipped for 6MV and 15MV X-ray beam was investigated to search for the optimal correction factor. Materials and Methods : The field size factors, beam quality and dose distribution were measured by using 6MV, 15MV X-ray Field size factors were measured from $3{\times}3cm^2$ to $35{\times}35cm^2$ by using 0.6cc ion chamber (NE 2571) at Dmax. Beam qualities were measured at different field sizes, off-axis distances and depths. Isodose distributions at different off-axis distance using $10\times10cm^2$ field were also investigated and compared with symmetric field. Result: 1) Relative field size factors was different along lateral distance with maximum changes in $3.1\%$ for 6MV and $5\%$ for 15MV. But the field size factors of asymmetric fields were identical to the modified central-axis values in symmetric field, which corrected by off-axis ratio at Dmax. 2) The HVL and PDD was decreased by increasing off-axis distance. PDD was also decreased by increasing depth For field size more than $5{\times}cm^2$ and depth less than 15cm, PDD of asymmetric field differs from that of symmetric one ($0.5\~2\%$ for 6MV and $0.4\~1.4\%$ for 15MV). 3) The measured isodose curves demonstrate divergence effects and reduced doses adjacent to the edge close to the flattening filter center was also observed. Conclusion . When asymmetric collimator is used, calculation of MU must be corrected with off-axis and PDD with a caution of underdose in central axis.

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Influence of Couch and Collimator on Dose Distribution of RapidArc Treatment Planning for Prostate Cancer in Radiation Therapy (치료테이블과 콜리메이터가 전립선암 래피드아크 치료계획의 선량분포에 미치는 영향)

  • Kim, Hyung-Dong;Kim, Byung-Young;Kim, Sung-Jin;Yun, Sang-Mo;Kim, Sung-Kyu
    • Progress in Medical Physics
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    • v.23 no.2
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    • pp.99-105
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    • 2012
  • We investigated the influence of photon energy, couch and collimator angle differences between arcs on dose distribution of RapidArc treatment planning for prostate cancer. RapidArc plans were created for 6 MV and 10 MV photons using 2 arcs coplanar and noncoplanar fields. The collimator angle differences between two arcs were $0^{\circ}$, $15^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, $75^{\circ}$ and $90^{\circ}$. The plans were optimized using same dose constrains for target and OAR (organ at risk). To evaluate the dose distribution, plans were analyzed using CI (conformity index), HI (homogeneity index), QOC (quality of coverage), etc. Photon energy, couch and collimator angle differences between arcs had a little influence on the target and OAR. The difference of dosimetric indices was less than 3.6% in the target and OAR. However, there was significant increase in the region exposed to low dose. The increase of V15% in the femur was 6.4% (left) and 5.5% (right) for the 6 MV treatment plan and 23.4% (left), 24.1% (right) for the noncoplanar plan. The increase of V10% in the Far Region distant from target was 54.2 cc for the 6 MV photon energy, 343.4 cc for the noncoplanar and 457.8 cc for the no collimator rotation between arcs.

Calibration of Optically Stimulated Luminescent nanoDot Dosimeter for 6 MV Photon Beam (6 MV 광자 빔에 대한 광자극형광나노닷선량계의 교정)

  • Kim, Jongeon;Kim, Seonghu;Lee, Hyoyeong
    • Journal of the Korean Society of Radiology
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    • v.7 no.1
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    • pp.93-98
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    • 2013
  • The purpose of this study is to investigate the calibration of an optically stimulated luminescent nanoDot dosimeter(OSLnD) to 6 MV photon beam. Dose ranges of the calibration of linear and non-linear from the analysis of dose response of the OSLnD were decided. To evaluate the accuracy of calibration equation and the calibration, the sets of the calibration and quality control dosimeter were used to make. The calibrations were performed by the linear and the non-linear in the dose range of 0~300 cGy and 20~1300 cGy, respectively. The errors of the calibration were acquired less than 0.1% respectively from the measurement of the quality control dosimeters for the calibration of linear and the non-linear. This study provides the calibration equation of the OSLnD to the 6 MV photon beam.

A Measurement of Kerma and Absorbed Dose in Photon Fields (Photon Beam에 대한 Kerma와 흡수선량의 측정)

  • Kim, Sung-Hee;Shin, Seung-Aea;Chu, Sung-Sil
    • Journal of Radiation Protection and Research
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    • v.11 no.1
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    • pp.77-82
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    • 1986
  • Determination of the relation between the kerma(Kinetic Energy Released in Material) and the absorbed dose is one of the basic problems of dosimetry. Kerma and absorbed dose were measured for 6 MV X-ray from the high energy medical linear accelerator and $^{60}Co$ gamma-ray. The experimental results show that the absorbed dose in the transient equilibrium region practically coincide with the kerma in water and Al for $^{60}Co$. The maximum dose depths were $1.45g/cm^2$ for 6MV X-ray and $0.48g/cm^2\;for\;^{60}Co$ gamma-ray. The ratios of the absorbed dose at maximum build-up to the collision kerma at the surface, ($K^{att}$), were 0.949 for 6MV X-ray and 0.992 for $^{60}Co$ gamma-ray. No difference was found between water and Al when the standard field size was used. This results show that the dependence of $K^{att}$ on the material is very small.

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Survey on Virus Infection for Commercial Nursery Trees of Major Apple Cultivars in Korea (국내 유통 주요 사과나무 묘목의 바이러스 감염 실태)

  • Lee, Sung-Hee;Kwon, Yeuseok;Shin, Hyunman;Nam, Sang-Yeong;Hong, Eui Yon;Kim, Byeongkwan;Kim, Daeil;Cha, Byeongjin;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.23 no.4
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    • pp.355-362
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    • 2017
  • The 4 viruses, the Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple mosaic virus (ApMV) and 1 viroid, Apple scar skin viroid (ASSVd) are known major viral pathogens of apple trees in Korea. Infection degree of the 5 viral pathogens in the commercial nursery trees of major apple cultivars, 'Hongro', 'Fuji' and bud mutation of 'Fuji' was investigated. Infection ratio of the ACLSV, ASPV and ASGV for scion of an apple cultivar 'Hongro' were 100%, 81.3% and 100%, respectively. On the other hand, no infection for either ApMV and ASSVd detected. For the root stock of the cultivar, infection ratio of ACLSV, ASPV and ASGV showed 87.5%, 81.3% and 100% as well as ApMV and ASSVd were 12.5% and 6.3%, respectively. From the scion of apple cultivars 'Fuji' and bud mutation of 'Fuji', infection ratio of ACLSV, ASPV and ASGV showed 86.7%, 86.7% and 100%, respectively. Whereas, no infection for either ApMV or ASSVd detected. From the root stock of the cultivars, infection ratio of ACLSV, ASPV and ASGV showed 86.7%, 93.3% and 93.3% as well as ApMV and ASSVd were 12.5% and 6.3%, respectively. Result of our study indicates that most of commercial nursery apple trees were supplied with multiple infections by apple viruses causing potential losses for apple growers and, henceforth, agricultural policy for supply of the virus-free trees should be employed as soon as possible.

Effect of Transverse Magnetic Field on Build-up Region of 6 MV Photon Beam (6 MV 광자선의 선량 상승 영역에 대한 자기장 영향)

  • Shin, Seong Soo;Choi, Wonsik;Ahn, Woo Sang;Kwak, Jungwon
    • Journal of the Korean Magnetics Society
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    • v.27 no.1
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    • pp.18-22
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    • 2017
  • The purpose of this study was to present an improved method of dose modulation over the increase of build-up for existing 6 MV photon beam. Two neodymium permanent magnets with a strength of 0.5 T (Tesla) were applied with a magnetic field perpendicular to the photon beam. The effects of dose on build-up region with or without the magnetic field were measured according to the magnet-to-magnet distance (MMS) and the magnet-to-surface distance (MSD). For MMS = 6 cm and MSD = 2.5 cm, $D_{0mm}$, $D_{2mm}$, $D_{5mm}$, and $D_{10mm}$ showed improved doses of 6.8 %, 14.6 %, 6.9 %, and 2.1 %, respectively, as compared with 6 MV open beam. In this study, the device with low strength magnetic field can be applied directly to the outside of the human body when the target volume located close to the skin is delivered with radiation. It is expected that the method of build-up modulation using a low strength magnetic field will be feasible in the clinical applications.