• Journal of Aquaculture
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• v.10 no.3
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• pp.363-371
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• 1997

Backcross hybridization between female cyprinid loach(Misgurnus anguillicaudatus) any male mud loach (M. mizolepis)$\times$cyjprinid loach hybrid were made, and its effect on early survival, cytogenetic traits, and gonad development were examined. Mean fertilization rate and hatching success of backcross hybrids were similar to those found in the maternal species, the cyprinid loach. However, the backcross hybrids revealed significantly improved early survival up to yolk sac absorption (86.0%) compared to cyprinid loach (0%) under low culture temperature ($15^{\circ}C$). the erythrocytic size and DNA content of backcross hybrids were nearly intermediate to those of the parents. Karyological analysis of backcross hybrids displayed two kinds of modal chromosome number of 2n=48 or 2n=49. Growth of backcross hybrids over 6 months after hatching was proven to be intermediate between their parental species. Although backcross hybrids had intermediate values between those of thier parents in most morphometric traits, overall external morphology of backcross hybrids was morer simlar to cyprinid loach than mud loach$\times$cyprinid loach hybrid. Histological examination of gonads at 2 and 4 months of age revealed that no female was obseved in backcross hybrid groups, suggesting the possibility of production of monosex male population.

• Korean Journal of Environmental Biology
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• v.18 no.3
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• pp.323-330
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• 2000

Early life history and spawning for Odontobutis interrupta were observed in the laboratory during May-August 1999 with a condition of natural habitats in the field. Optimal water temperature for spawning was between 17.5 and 22.$0^{\circ}C$ and appropriate water depth and current velocity in the natural habitat ranged 0.3-0.6 m and 0.1-0.3 m/sec, respectively. Ovary maturation index peaked at about 100mm in the total length and their values gradually decreased after the size. Male fishes showed a territory and courtship behavior to adult females and the males frequently pushed upper-ventral part of females for egg releases. After females spawned, the males guarded the egg masses and supplied dissolved oxygen using pectoral fins. According to observation of egg development in the laboratory, blastodisc formed in 1hr 17 min after the fertilization, cleavaging at 36-minute interval regularly. Blastulation occurred in 7 hr 12 min after the fertilization, with gastrulation after 11 hr 11 mins and formation of york plug after 32 hr 48 min. Embryo was formed in 33 hr 45 min after fertilization and optic vesicles appeared in 47 hr 27 mins when 30-31 somites were formed. Cardiac primordium was formed in 65 hr 15mins and its beat averaged 44- 48 time/min. Pectoral fins were formed in 138 hr 40 min, air-bladder and black vesicles were observed in lower portion of young fish. Embryo hatched from she-11 membrane after about 10 days and juvenile was 5.8$\pm$0.2mm in total length 3.0$\pm$0.5mg in weight.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.199-204
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• 2010

A new tall fescue variety (Festuca arundinacea Schreb.) named "Greenmaster" was developed by the National Institute of Animal Science, RDA, at Suwon from 1999 to 2007. For synthetic seed production of this new variety, 5 superior clones, EFa9111, EFa9122, EFa9211, EFa9225, and EFa9234 were selected and polycrossed. The agronomic growth characteristics and forage production capability of the seeds were studied at Suwon from 2003 to 2004, and regional trials were conducted in Suwon, Pyungchang, Jeju, and Ikcsan from 2005 to 2007. Greenmaster showed enhanced winter hardiness, disease resistance, and regrowth ability as compared to Fawn. The dry matter yield of Greenmaster was 11% higher as 19,156 kg/ha than that of Fawn. However, the nutritive value of both varieties was similar.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.275-280
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• 2005

This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 2002. Hwasan 104 as a tetraploid variety was dark green in leaf color and had semi-prostrate and medium growth habit in late autumn and early spring, respectively. Hwasan 104 was 19th May in heading date as a late-heading variety. Especially, Hwasan 104 was broader in flag leaf and longer 7 cm in plant length, and thicker in stem width than those of control variety, Hwasan 101. Cold tolerance of Hwasan 104 was better than that of Hwasan 101 registered as a cold-tolerant variety. Dry matter (DM) yield of Hwasan 104 was $5\%$ more than DM 9,348 kg/ha of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient of Hwasan 104 were 74.3 and $63.1\%$ which are 3.3 and $1.5\%$ lower than those of Hwasan 101, respectively. Acid detergent fiber and neutral detergent fiber of Hwasan 104 were 32.6 and $55.8\%$ which are 1.9 and $2.4\%$ higher than those of Hwasan 101, respectively.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.81-88
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• 1998

The Southeast Asian javanica rice variety Tinawen was investigated for efficient protoplast culture and plant regeneration from cell suspension-derived protoplasts using a feeder cell culture method. Feeder cells of both Lolium multiflorum and Oryza ridleyi, either alone, or in combination, were employed and plants were regenerated from protoplast-derived colonies on several plant regeneration media. Dehydration of protoplast-derived colonies was also investigated as a means of enhancing plant regeneration. In the presence of L. multiflorum or O. ridleyi feeder cells, the protoplast plating efficiency ranged from 0.09% to 1.48%, depending on the feeder cell type and the age of the cell suspension. L. multiflorum feeder cells induced approximately 6-fold higher plating efficiency compared with those of O. ridleyi. The plant regeneration frequencies were 19.3-31.7% with L. multiflorum, 13.0-18.0% with O. ridleyi and 18.0-22.0% with a mixture of both in various plant regeneration media when protoplast-derived colonies were dehydrated, while for the non-dehydrated colonies, the values were 2.0-7.0%, 3.0-5.0% and 0-4.0%, respectively. Flow cytometric analysis of 34 protoplast-derived plants showed that the majority of plants were diploids and only 2 plants were tetraploids. The plants which were transferred to glasshouse were fertile.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.1
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• pp.114-120
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• 1998

The spawning behavior, development of eggs and larvae of the Korea freshwater goby, Rhinogobius brunneus (Temminck et Schlegel) were studied. The eggs were spawned as a one-layer mass, hanging from the underside of a small pebble, and guarded by one male. The eggs were elliptic, about 1.48 mm in length and 0.65 mm in breadth, with a round top and a somewhat flat base with glutinous fibers. Hatching in the indoor tank with $17.0^{\circ}C$ in mean water temperature started from the 146 hours after fertilization. In the late developing stages, the embryo moved and freely changed its head to face the free tip of the chorion (normal egg) or to the basal end with a boundle of adhesive filaments (agrippa egg). Newly-hatched larvae were $3.10\~3.30$ mm in total length (mean: 3.22 mm), and mouth and anus were not yet open. Melanophores were present on the air- bladder, around the anus, and on the ventral part of the caudal region. The larva $3\~4$ days old transformed to postlarval stage, and they were $3.30\~3.85$ mm in total length (mean: 3.60 mm). As yolk sac and oil globules werw nearly absorbed, mouth and anus were open, and they fed rotifers actively. In $20\~22$ days after hatching the larvae grew to 5.85 mm in 71, and the caudal notochord flex at $45^{\circ}$.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.64-64
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• 2003

대구 인공종묘 생산 기술 개발의 일환으로 수정란에 기생하여 폐사를 유발하는 세균 및 세균 감염 경로를 파악함으로써 수정란의 생존율과 부화율 향상을 위하여 본 실험을 실시하였다. 세균 감염 경로를 파악하기 위하여 난소와 정소 자체 세균 감염 여부를 조사하였다. 일반배지 (BHIA)와 비브리오 선택배지 (TCBS)를 사용하여 세균검사를 실시하였다. 난소의 세균 검사는 생식소 내부를 일부 절개한 후 멸균 roop를 찔러 세균을 검사하였고, 정소는 채정하기 전 복부를 절개하여 멸균 roop로서 적출하여 배지에 도말한 후 배양하였다. 수정란 내 세균 수는 수정란을 각각 20개씩 샘플하여 난 외부에 기생하는 세균을 제거하기 위하여 난소독제로 이용되고 있는 benzalkonium 0.1%로 1분간 소독한 후 멸균 생리식염수로 3회 세척하여 호모게나이즈하여 검사하였다. 호모게나이즈한 액 중 100${\mu}\ell$를 pipetting하여 일반배지(BHIA)에 도말하여 인큐베이터에서 배양하였다. 난소와 정소 내에서 세균이 검출됨에 따라 수정 시 세균 감염을 억제하기 위하여 자외선 살균해수와 일반해수를 수정액으로 사용하여 발생율을 비교 시험하였다. 수정 시간은 1분으로 동일하게 적용하였으며, 수정 용기는 멸균 처리된 일회용 100$m\ell$ 플라스틱 용기를 사용하였다. 수정 후 과다한 정자를 제거하기 위한 세란은 1$\ell$ 멸균 비이커에서 5회 30분 가량 실시하여 정자를 제거하였고 멸균 봉으로 저어주면서 수정란의 점착력을 제거하였다. 수정란은 100$\mu\textrm{m}$ 그물망으로 수정란 유실을 방지한 플라스틱 용기에 수용하여 유효수량 270$\ell$ FRP 수조에 수용하여 3$\ell$/min 환수하였고, 수온은 자연수온 1$^{\circ}C$로 유지하였다. 발생율은 만능투영기로 3회 측정하였다. 수정 후 세균과 기생충에 의한 수정란의 폐사를 억제하기 위하여 수정 직후, 수정 후 1일, 2일째 oxytetracycline과 iodine 처리에 따른 발생율 변화를 조사하였다. 발생율은 만능투영기로 조사하였고, 시험구별로 3회 측정하였다. 경과 일수별로 약제 처리는 약제 미처리 수정란 중 정상적인 발생이 이루어지고 있는 것을 선별하여 조사하였다. 약제 처리에 따른 배체 발생 단계는 수정 후 1일째는 상실기, 2일째는 포배기였다. 수정란 및 대구 자어에서 분리된 V. splendidus 에 의한 폐사를 예방하기 위해 in vivo에서 oxytetracycline 외 5종의 항생제를 대상으로 96well plate에서 최고 농도 250ppm부터 2 fold로 단계 희석하여 $25^{\circ}C$에서 48시간 배양하여 MIC를 조사하였다. 세균 감염경로 파악을 위하여 난소, 정소 및 정자에서 세균을 분리한 결과 일반배지 및 비브리오 선택배지에서 모두 균이 검출되었고, 균 동정 결과 터봇 자어에서 검출된 것으로 보고된(Gatesoupe et al., 1999) V. splendidus로 나타났다. 수정액과 정자 및 미수정란의 세균 분리 결과 일반배지에서 3$\times$10/ml ~ 7$\times$10/ml로 균이 검출되었다. 수정액을 일반해수와 자외선 살균 해수를 사용하여 발생율을 비교한 결과 수정 후 3일째 발생율은 자외선 살균해수 72.3%, 일반해수 52.7%였으며, 수정 후 7일째 40.9%와 25.1%로 자외선 살균해수가 유의적으로 높게 나타났다. 수정 후 경과 일수별로 oxytetracycline과 Iodine을 처리한 결과 수정 직후 처리한 시험구는 7일째 19.8%와 18.9%로 대조구 23.1%와 유의적인 차이가 없는 것으로 나타났다. 수정 후 1일째 처리한 시험구는 54.5%와 56.8%, 수정후 2일째 처리구는 47.9%와 50.6%로 두 시험구 모두 대조구와 수정 직후 처리구보다 유의적으로 높게 나타났다. 수정란 및 대구 자어에서 분리된 V. splendidus 에 의한 폐사를 예방하기 위해 in vivo에서 항생제 종류별 MIC 조사 결과 oxytetracycline이 0.48ppm으로 가장 효과가 높은 것으로 나타났다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.386-398
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• 2020

Wheat is one of the world's top three crops and is an important staple crop, accounting for 20% of the nutrient calories consumed by the world's population. However, due to its complex heterogeneous hexaploid chromosomes and vast genome of approximately 16 Gb, compared to those of other crops, molecular biology and biotechnology studies on wheat are lacking. In recent years, wheat genome analysis has been performed using the latest next-generation sequencing technology so that useful genes can be easily obtained, and wheat biotechnology research is accelerating in various fields. In this review, wheat transformation, an indispensable technique for developing new functional biotech wheat by revealing the function of wheat genes, is described in detail. In addition, the latest research results for overcoming plant diseases, abiotic stresses, and wheat-related diseases that are difficult to solve by classical breeding through wheat transformation and biotechnology are described.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.56-62
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• 2013

We bred new mulberry cultivar through local adaptability test, which was registered as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Chuncheon and Jinju) for four years since 2003. This is tetraploidy variety belonging to (Morus Lhou(Ser.) Koidz.) made by colchicine treatment on growing point of winter buds. Daejappong was high yielding in productivity by 3% compared to control cultivar "Chungilppong" for two years.The sugar contents of Daejappong was higher than that of control cultivar 'Chungilppong(Morus alba L.)'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.141-149
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• 2020

Solanum hougasii, one of the wild Solanum species, has been widely used in potato breeding since it exhibits excellent resistance to diverse important pathogens. S. hougasii can be directly crossed with the cultivated tetraploid potato (S. tuberosum) owing to its EBN (Endosperm Balanced Number) value of 4, which is same as that of S. tuberosum although it is an allohexaploid. In this study, the complete chloroplast genome sequence of S. hougasii was obtained by next-generation sequencing technology, and compared with that of the chloroplast genome of seven other Solanum species to identify S. hougasii-specific PCR markers. The length of the complete chloroplast genome of S. hougasii was 155,549 bp. The structural organization of the chloroplast genome in S. hougasii was found to be similar to that of seven other Solanum species studied. Phylogenetic analysis of S. hougasii with ten other Solanaceae family members revealed that S. hougasii was most closely related to S. stoloniferum, followed by S. berthaultii, and S. tuberosum. Additional comparison of the chloroplast genome sequence with that of five other Solanum species revealed five InDels and 43 SNPs specific to S. hougasii. Based on these SNPs, four PCR-based markers were developed for the differentiation of S. hougasii from other Solanum species. The results obtained in this study will aid in exploring the evolutionary and breeding aspects of Solanum species.