• Title/Summary/Keyword: 5S Method

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Phylogenetic Study of Ganoderma applanatum and Schizopora paradoxa Basd on 5S rRNA Sequences (5S rRNA 염기서열에 으한 잔나비걸상과 좀구멍버섯의 계통학적 연구)

  • Kim, Hak-Hyun;Jung, Hack-Sung
    • Korean Journal of Microbiology
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    • v.32 no.3
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    • pp.177-181
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    • 1994
  • The sequences of the cytoplasmic 5S rRNAs(EMBL accession number X73589 and X73890) from two polupores, Ganoderma applanatum and Schizopora paradoxa, were determined by the direct chemical method for sequencing RNA and compared to the sequences of 9 reported mushrooms. 5S rRNAs of Ganoderma applanatum and Schizopora paradoxa consisted of 118 bases and fit the secondary structure model of the 5S rRNAs of basidiomycetes proposed by Huysmans et al. Based on Kimura’s K_nuc values, the closest fungus to Ganoderma applanatum was Ceratobasidium cornigerum and the one to Schizopora paradoxa was Bjerkandera adusta. When the secondary structures of 5S rRNAs of 11 mushrooms were compared the base substitution occurred at helix regions more than at loop regions. When a phylogenetic tree was constructed using the Neighbor program of the PHYLIP package, it partially discriminated and separated the mushrooms of the Hymenomycetes by the order.

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Morphology Control of NTO Crystals with Various Recrystallization Techniques

  • Lee, H.Y.;K.K. Koo;Kim, K.J.;G.B. Lim;W.B. Jeong;Kim, S.H.;Kim, H.S.;Park, B.S.
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1997.10a
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    • pp.123-127
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    • 1997
  • Various recrystallization techniques has been applied to control morphology and size of NTO(3-nitro-1, 2, 4-triazole-5-one) crystals. With cooling method, it was found that the size of NTO at aqueous solution was controlled in the range of 5 to 500 $\mu\textrm{m}$. The spherical or cubic shapes of particles were obtained by adjusting operating conditions. Hexagonal and cubic shaped crystals of NTO were also obtained by sonication and evaporative method using aqueous solution of NTO. Their particle sizes were ranged 20 to 30 $\mu\textrm{m}$. In gas anti-solvent method with NTO/DMF and NTO/DMSO solutions, cubic type of NTO was obtained and the range of their sizes was 0.5-2$\mu\textrm{m}$.

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Direct and Quantitative Analysis of Salmonella enterica Serovar Typhimurium Using Real-Time PCR from Artificially Contaminated Chicken Meat

  • Park, Hee-Jin;Kim, Hyun-Joong;Park, Si-Hong;Shin, Eun-Gyeong;Kim, Jae-Hwan;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.18 no.8
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    • pp.1453-1458
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    • 2008
  • For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with $4.5{\times}10^5$ to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.

A Comparative Study on Stress and Coping Method of Psychiatric Patients and Non-Psychiatric Peoples (정신질환자와 비정신질환자의 스트레스 및 그 적응방법에 대한 비교연구)

  • 고성희
    • Journal of Korean Academy of Nursing
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    • v.9 no.1
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    • pp.23-29
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    • 1979
  • This study on stress and coping method of psychiatric patients and non-psychiatric peoples was applied to people who was divided into two groups from Sep. 25 to Oct. 3, 1978, One is hospitalized patients in psychiatric wards of four hospitals in Seoul. The other is inhibitants in two Dongs of Seoul, This study purposed to the identification of the difference of stressful events’numbers, the severity of stress, and difference of coping methods between psychiatric patients and non-psychiatric peoples. Two instruments are used in this study. The first one to measure stress, is Holmes & Rahe (1967)'s SRRQ (Social Readjustment Rating Questionnaire), which is amended, added or omitted through preliminary test, so that it consists of 48 items. The second one is for evaluating coping method on stress. It consists of 34 items amended through preliminary test after consideration of related literature review and survey on the basis of J.M.Bell (1977)'s“ 18-item-Questionnaire”. The materials were analyzed by S.P.S.S. Program. The results of analysis are as follows: 1. There was no significant difference in stressful event's numbers between psychiatric patients and non-psychiatric peoples (P > 0.5). 2. Psychiatric patients had higher severity of stress than non-psychiatric peoples (P < 0.5). 3. Psychiatric patients took more short-term coping methods than non-psychiatric peoples (p < 0.5).

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Comparison of various dietary intake assessment method in monitoring the adherence to fat-modified diet (저지방.저콜레스테롤 식이요법 환자군의 식이섭취실태 평가법에 대한 비교 연구)

  • 장남수
    • Journal of Nutrition and Health
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    • v.22 no.6
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    • pp.438-446
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    • 1989
  • This study was designed to compare various dietary assessment methods and to determine the appropriate method that would be reliable for monitoring the adherence to a fat-modified diet. Dietary intake data was collected from the twenty-two (12 males, 10 females), type IIa hypercholesterolemic patients by the 24-hour dietary recall and the food records of various durations(9, 7, 4, 3days). Intakes of energy, protein, fat, carbohydrates, cholesterol, polyunsaturated fatty acids, saturated fatty acids, monounsaturated fatty acids, P/S ratio, and alcohol were analyzed by a computer-assisted method using the Natinal Heart, Lungand Blood Institute Nutrition Data System. The nutrient intake data of the 24-hour recall method were consistently higher than tat of the food record method. In all subjects, food record keeping of the 7 consecutive days provided acceptable dietary data (within 5% difference) when compared to that of the 9 consecutive days. Food records of 4 consecutive days however, were found to be adequate for female subjects, showing a sex difference. Except for P/S ratio, nutrient intakes did not vary significantly between weekdays and weekend-days. Among other components, alcohol and P/S ratio were shown to be the two most varied components in this study.

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Synchronization and Performance Evaluation of MIMO/F-OFDM Systems for 5G Mobile Communications (5세대 이동통신용 다중안테나/F-OFDM 시스템에서의 동기 방식과 성능 평가)

  • An, Changyoung;Ryu, Heung-Gyoon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.42 no.1
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    • pp.31-38
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    • 2017
  • In this paper, we have designed MIMO system using F-OFDM modulation. And then, we have evaluated and analyzed synchronization performance of the system. In this paper we have considered Schmidl's method, Minn's method, and Park's method. As simulation results, Schmidl's method has wide plateau of timing metric and Park's method has impulse-shape timing metric. Also, we can confirm that timing metric characteristic of synchronization estimator can be degraded by adjusting filter length of F-OFDM system. Especially, we can confirm that timing metric of synchronization estimator is shifted according to filter length of MIMO system using F-OFDM modulation and this timing metric movement can be compensated by using designed filter length.

Implementation and Performance Analysis of Single I/O Space Service for Cluster Computers (클러스터 컴퓨터를 위한 단일 I/O 공간 서비스의 구현 및 성능분석)

  • Kim, Tae-Kyu;Kim, Bang-Hyun;Kim, Jong-Hyun
    • The KIPS Transactions:PartA
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    • v.13A no.6 s.103
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    • pp.517-524
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    • 2006
  • In cluster computers, it is essential to Implement the single I/O space(SIOS) supporting integrated I/O substructure to efficiently process I/O intensive applications. SIOS service provides with global I/O address space to directly access peripherals and hard disks in its own or remote nodes from any node in the cluster computer In this thesis, we propose the implementation method of SIOS in Linux clusters by using only freewares. This method is implemented at device driver level that uses Enhanced Network Block Device(ENBD) and file system level that uses S/W RAID and NFS. The major strengths of this method are easiness of implementation and almost no cost due to using freewares. In addition, since freewares used are open sources, it is possible to apply this method to other platforms with only slight modification. Moreover, experiments show that I/O throughputs are up to 5.5 times higher in write operations and approximately 2.3 times higher in read operations than those of CDD method that uses the device driver developed at kernel level.

ADAPTATION OF THE MINORANT FUNCTION FOR LINEAR PROGRAMMING

  • Leulmi, S.;Leulmi, A.
    • East Asian mathematical journal
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    • v.35 no.5
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    • pp.597-612
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    • 2019
  • In this study, we propose a new logarithmic barrier approach to solve linear programming problem using the projective method of Karmarkar. We are interested in computation of the direction by Newton's method and of the step-size using minorant functions instead of line search methods in order to reduce the computation cost. Our new approach is even more beneficial than classical line search methods. We reinforce our purpose by many interesting numerical simulations proved the effectiveness of the algorithm developed in this work.

Free Vibration Analysis of Axisymmetric Conical Shell

  • Choi, Myung-Soo;Yeo, Dong-Jun;Kondou, Takahiro
    • Journal of Power System Engineering
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    • v.20 no.2
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    • pp.5-16
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    • 2016
  • Generally, methods using transfer techniques, like the transfer matrix method and the transfer stiffness coefficient method, find natural frequencies using the sign change of frequency determinants in searching frequency region. However, these methods may omit some natural frequencies when the initial frequency interval is large. The Sylvester-transfer stiffness coefficient method ("S-TSCM") can always obtain all natural frequencies in the searching frequency region even though the initial frequency interval is large. Because the S-TSCM obtain natural frequencies using the number of natural frequencies existing under a searching frequency. In this paper, the algorithm for the free vibration analysis of axisymmetric conical shells was formulated with S-TSCM. The effectiveness of S-TSCM was verified by comparing numerical results of S-TSCM with those of other methods when analyzing free vibration in two computational models: a truncated conical shell and a complete (not truncated) conical shell.

Object-Action and Risk-Situation Recognition Using Moment Change and Object Size's Ratio (모멘트 변화와 객체 크기 비율을 이용한 객체 행동 및 위험상황 인식)

  • Kwak, Nae-Joung;Song, Teuk-Seob
    • Journal of Korea Multimedia Society
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    • v.17 no.5
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    • pp.556-565
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    • 2014
  • This paper proposes a method to track object of real-time video transferred through single web-camera and to recognize risk-situation and human actions. The proposed method recognizes human basic actions that human can do in daily life and finds risk-situation such as faint and falling down to classify usual action and risk-situation. The proposed method models the background, obtains the difference image between input image and the modeled background image, extracts human object from input image, tracts object's motion and recognizes human actions. Tracking object uses the moment information of extracting object and the characteristic of object's recognition is moment's change and ratio of object's size between frames. Actions classified are four actions of walking, waling diagonally, sitting down, standing up among the most actions human do in daily life and suddenly falling down is classified into risk-situation. To test the proposed method, we applied it for eight participants from a video of a web-cam, classify human action and recognize risk-situation. The test result showed more than 97 percent recognition rate for each action and 100 percent recognition rate for risk-situation by the proposed method.