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Biosorption of Lead(II) by Arthrobacter sp. 25: Process Optimization and Mechanism

  • Jin, Yu;Wang, Xin;Zang, Tingting;Hu, Yang;Hu, Xiaojing;Ren, Guangming;Xu, Xiuhong;Qu, Juanjuan
    • Journal of Microbiology and Biotechnology
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    • 제26권8호
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    • pp.1428-1438
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    • 2016
  • In the present work, Arthrobacter sp. 25, a lead-tolerant bacterium, was assayed to remove lead(II) from aqueous solution. The biosorption process was optimized by response surface methodology (RSM) based on the Box-Behnken design. The relationships between dependent and independent variables were quantitatively determined by second-order polynomial equation and 3D response surface plots. The biosorption mechanism was explored by characterization of the biosorbent before and after biosorption using atomic force microscopy (AFM), scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The results showed that the maximum adsorption capacity of 9.6 mg/g was obtained at the initial lead ion concentration of 108.79 mg/l, pH value of 5.75, and biosorbent dosage of 9.9 g/l (fresh weight), which was close to the theoretically expected value of 9.88 mg/g. Arthrobacter sp. 25 is an ellipsoidal-shaped bacterium covered with extracellular polymeric substances. The biosorption mechanism involved physical adsorption and microprecipitation as well as ion exchange, and functional groups such as phosphoryl, hydroxyl, amino, amide, carbonyl, and phosphate groups played vital roles in adsorption. The results indicate that Arthrobacter sp. 25 may be potentially used as a biosorbent for low-concentration lead(II) removal from wastewater.

CNT 성장시 Ni 및 Co 촉매의 조성비에 따른 CNT-W 팁의 전계방출 특성 분석 (Field emission properties of CNT-W tips as a function of the composition ratio of Ni and Co catalysts in CNT growth)

  • 김원;윤성준;김영광;김종필;박창균;박진석
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.1269-1270
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    • 2007
  • Carbon nanotubes (CNTs) are directly grown on W-tips at $700^{\circ}C$ using an ICP-CVD method. Sharpening of W-tip is done by electrochemical etch and their diameters are limited to range from $3{\mu}m$ to $5{\mu}m$. Catalysts for CNTs growth are formed by RF and DC co-sputtering systems using Ni and Co. The composition ratio of Ni and Co has been evaluated by energy dispersive x-ray spectroscopy (EDS). The micro-images of CNTs are monitored by field emission scanning electron microscope (FESEM). It is observed from Raman study that the intensity of the D-peak is increased by increasing the amount of Co catalyst. Furthermore, the measurement of field emission properties of CNTs show that the CNT grown on a single Co catalyst possess the greatest performance such as $V_{th}$=1,115V and $I_{max}=164{\mu}A$.

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Characteristics of NbN Films Deposited on AISI 304 Using Inductively Coupled Plasma Assisted DC Magnetron Sputtering Method

  • Jun, Shinhee;Kim, Junho;Kim, Sunkwang;You, Yong Zoo;Cha, Byungchul
    • 한국표면공학회지
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    • 제46권5호
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    • pp.187-191
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    • 2013
  • Niobium nitride (NbN) films were deposited on AISI 304 stainless steels by inductively coupled plasma (ICP) assisted dc magnetron sputtering method at different ICP powers, and the effects of ICP power on the phase formation, mechanical and chemical properties of the films were investigated. X-ray diffraction analysis (XRD) and field emission scanning electron microscopy (FESEM) were used to analyze the crystal structure and micro-knoop hardness was used to measure the hardness of the films. Also, 3-D mechanical profiler and a ball-on-disk wear tester were used to measure the thickness of the films and to estimate wear characteristics, respectively. The thickness of the films decreased but their hardness increased with increasing ICP power, and it was confirmed that only cubic ${\delta}$-NbN(200) remained at high ICP power. At lower ICP powers, a mixture of the hexagonal ${\delta}^{\prime}$-NbN and cubic ${\delta}$-NbN phases was obtained in the films and the hardness decreased. The corrosion potential value increased gradually with increasing ICP power, but the changes of ICP power did not significantly influence the overall corrosion resistance.

감마선을 이용한 조직공학용 젤라틴이 개질된 미생물 셀룰로오스 지지체의 제작 및 특성 (Preparation and Characterization of Gelatin-immobilized Bacterial Cellulose Scaffold for Tissue Engineering Using Gamma-ray Irradiation)

  • 최종배;정성린;권희정;박종석;노영창;최영훈;박경진;박만용;신흥수;임윤묵
    • 방사선산업학회지
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    • 제6권2호
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    • pp.159-164
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    • 2012
  • Bacterial cellulose (BC) is generated from citrus gel by Gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure, high-water contact and the ultrafine highly nanofibrous structure of mimic natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation, and then immobilized gelatin onto AAc-g-BC. The characterization of scaffolds was performed by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), toluidine blue O (TBO) assay. Morphology of gelatin and AAc incorporation onto BC nanofibers did not changed. Our study suggests that gelatin-immobilized BC nanofibers scaffold has a potentiality to fabricate 3D nanofibrous scaffolds for tissue engineering.

척추교정 티타늄 앵커나사 파단 손상원인 분석 (Failure Analysis of Ti alloy Screws in Fixing Fractured Spines)

  • 최병학;김문규;김승언;심윤임;이영진;정효태;최원열
    • 대한금속재료학회지
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    • 제49권12호
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    • pp.983-988
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    • 2011
  • Failure analyses of the screws in spinal fixation devices were carried out. The fractured screws were retrieved from a patient who had spinal surgery in the thoracic vertebrae from number 10 to 15. The failure occurred one month after the removal of the braces. Microstructures and fracture surfaces were examined by optical and scanning electron microscopy. The microstructures of the screws corresponded to annealed Ti-6Al-4V bar. However, in the vicinity of the screw surface, there was an insufficient number of fine precipitates. Fracture surfaces showed typical fatigue failure modes. Regarding the fact that no machining defects were detected, fatigue crack initiation might have been caused by the lack of precipitates near the screw surfaces. Only the fourth of five fixed screws was severely stress-concentrated by the action of the spinal bones, while the stress of the 4th screw was decreased to half of its acceptable level when the screw was supplemented by one more, which might have been fixed in the 6th vertebra under the 5th position by the switching of its position. The stress simulation was conducted by ANSYS with 3D CAD of PRO/E in order to understand the stress concentration behavior and to provide an effective spinal surgery guide.

건축물 평면 형상에 대한 형상-to-BIM 맵핑 규칙 정의 (Geometry-to-BIM Mapping Rule Definition for Building Plane BIM object)

  • 강태욱
    • 한국산학기술학회논문지
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    • 제20권10호
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    • pp.236-242
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    • 2019
  • 최근 유지보수 등의 목적으로 다양한 건설 및 건축 분야에서 스캔 프로젝트가 수행되고 있다. 스캔된 결과로 생선된 포인트 클라우드는 스캔 대상물을 표현하는 수많은 점들로 구성되어 있다. 이런 스캔 데이터에서 치수를 포함한 필요한 정보를 추출하는 과정을 역설계라 한다. 포인트 클라우드를 BIM으로 모델링하는 역설계 과정은 수많은 수작업이 포함되어 있다. 시간이 많이 소모되는 역설계 작업 특성상 설계변경 같은 재작업 요청이 발생되면 비용은 기하급수적으로 증가한다. 역설계 자동화 기술이 이런 문제를 개선하는 데 도움을 줄 수 있다. 하지만, 역설계 산출물은 유스케이스에 따라 가변성을 가지며, 산출물의 종류와 상세수준은 달라질 수 있다. 이런 점을 고려해, 본 연구는 건축물 평면객체 기본 형상(primitive geometry)에서 BIM객체로 자동 맵핑하는 G2BM(Geometry-to-BIM mapping) 규칙 정의 방법을 제안한다. G2BM는 사용자 활용사례 가변성을 고려한 건축물 평면 BIM객체 역설계 프로세스 정의와 사용자화 방안을 제안한다. 그리고, 프로토타입을 통해 이에 대한 효과를 확인한다.

Morphological and Molecular Characterization of Desportesius invaginatus (Nematoda: Acuariidae) from Egretta garzetta and Bubulcus ibis in Korea

  • Lee, Seo-In;Hong, Eui-Ju;Kim, Hyeon-Cheol;Ryu, Si-Yun;Park, Bae-Keun
    • 한국임상수의학회지
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    • 제38권2호
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    • pp.75-81
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    • 2021
  • An aquariid nematode, Desportesius invaginatus, was found in the proventriculus of an Egretta garzetta and a Bubulcus ibis from Chuncheon in the Republic of Korea. The worms were identified by light and scanning electron microscopy based on important taxonomic characteristics (body length, esophagus length, cordons, spicules, caudal alae of males, position of the vulva) and then phylogenetically analyzed using the 18S rRNA encoding gene. The nematodes were characterized by a body length of 7.0-8.0 mm in males and 10.2-13.1 mm in females, and two pairs of cordons recurrent in the anterior direction, and cordons were anastomosed by a longitudinal cuticular ridge that externally delimits a longitudinal canal. The widest cuticular plates of cordons bears over 20 posterior spines. The length of the spicules in males was also significantly different. The right spicule measured 742-821 (794) ㎛ in length and 40-45 (42) ㎛ in width, and the left spicule measured 493-556 (541) ㎛ in length and 11-13 (12) ㎛ in width. The caudal alae of males are inflated and vesicular in appearance. The vulva was situated at 56-71 (58.3) ㎛ from the posterior extremity. Although the 18S rRNA sequences of worms were similar to the Synhimantus species, some genetic divergences were observed in comparison. In this study, the worms were recognized with genus Desportesius because genus Desportesius was considered a subgenus of Synhimantus. This is the first record of D. invaginatus in the Republic of Korea.

차량도어 조립공차 예측기술 개발 (An Advanced Prediction Technology of Assembly Tolerance for Vehicle Door)

  • 정남용;조진형;오현승;이세재
    • 산업경영시스템학회지
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    • 제41권4호
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    • pp.91-100
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    • 2018
  • The setting of values on door hinge mounting compensation for door assembly tolerance is a constant quality issue in vehicle production. Generally, heuristic methods are used in satisfying appropriate door gap and level difference, flushness to improve quality. However, these methods are influenced by the engineer's skills and working environment and result an increasement of development costs. In order to solve these problems, the system which suggests hinge mounting compensation value using CAE (Computer Aided Engineering) analysis is proposed in this study. A structural analysis model was constructed to predict the door gap and level difference, flushness through CAE based on CAD (Computer Aided Design) data. The deformations of 6-degrees of freedom which can occur in real vehicle doors was considered using a stiffness model which utilize an analysis model. The analysis model was verified using 3D scanning of real vehicle door hinge deformation. Then, system model which applying the structural analysis model suggested the final adjustment amount of the hinge mounting to obtain the target door gap and the level difference by inputting the measured value. The proposed system was validated using the simulation and showed a reliability in vehicle hinge mounting compensation process. This study suggests the possibility of using the CAE analysis for setting values of hinge mounting compensation in actual vehicle production.

DN 250만 250℃고온 스팀환경에서 운전되는 단열 브러쉬 실 마모효과에 관한 실험적 연구 (Experimental Study on the Wear Effects of a Brush Seal in DN 2.5million in a 250℃ High - temperature Steam Environment)

  • 하윤석;하태웅;이용복
    • Tribology and Lubricants
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    • 제35권2호
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    • pp.99-105
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    • 2019
  • This study presents an experimental investigation of the wear and oxidation of the bristles of a brush seal in a super-heated steam environment. We construct a model reflecting normal force and radial interference to predict the amount of wear. To monitor the volume loss of the bristle induced by the swirl phenomenon of the rotor, we measure the clearance between the rotor and the brush seal by using a non-contact 3-D device. We calculate the area by using the area-wise measurement method. Considering the obvious brush seal wear variables, we use two disks with different roughness($Ra=0.1{\mu}m$ and $100{\mu}m$) to determine the effect of roughness on wear. Considering an actual steam turbine, we utilize a steam generator and super-heater to generate a working fluid (0.95MPa, 523.15K) that has high kinetic energy. We observe the abrasion of the bristles in the hot steam environment through a scanning electron microscope image. This study also conducted energy dispersive X-ray (EDX) analysis for a qualitative evaluation of local chemistry. The results indicate that the wear and elimination of bristles occur on the disk with high roughness, and the weight increases due to oxidation. Furthermore these results, reveal that the bristle oxidation is accelerated more under super-heated steam conditions than under conditions without steam.

초박형 반도체 패키지의 EMC encapsulation을 위한 경화 공정 개발 (Development of Curing Process for EMC Encapsulation of Ultra-thin Semiconductor Package)

  • 박성연;온승윤;김성수
    • Composites Research
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    • 제34권1호
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    • pp.47-50
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    • 2021
  • 본 논문은 차량에 사용되는 B필러의 강화재를 기존의 스틸 소재에서 CFRP(Carbon Fiber Reinforced Plastics)와 GFRP(Glass Fiber Reinforced Plastics)로 대체하여 경량화하는 것이 목표다. 이를 위해서는 무게는 감소시키면서 기존 B필러를 대체할 수 있는 구조안정성을 확보해야 한다. 기존 B필러는 스틸 아우터(outer)를 포함하여 다양한 형상의 스틸 강화재로 구성되며, 이와 같은 스틸 강화재 중 2가지의 스틸 강화재를 복합재로 대체하고자 한다. 이와 같은 스틸 강화재는 강화재 각각을 따로 제작하여 용접을 통해 결합되지만, 복합재 강화재는 패치(patch) 형태의 CFRP와 리브(rib) 구조의 GFRP를 활용하여 압축과 사출 공정을 통해 한번에 제작된다. CFRP는 B필러의 고강도부에 부착되어 측면 하중에 저항하도록 하였으며, GFRP 리브는 위상 최적화(Topology optimization) 기법을 통해 비틀림과 측면 하중을 저항하도록 설계하였다. 구조해석을 통해 기존 스틸 강화재와 비교 분석을 수행하였고, 경량화율을 산출하였다.