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C/N/O/S stable isotopic and chemometric analyses for determining the geographical origin of Panax ginseng cultivated in Korea

  • Chung, Ill-Min;Kim, Jae-Kwang;Lee, Ji-Hee;An, Min-Jeong;Lee, Kyoung-Jin;Park, Sung-Kyu;Kim, Jang-Uk;Kim, Mi-Jung;Kim, Seung-Hyun
    • Journal of Ginseng Research
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    • v.42 no.4
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    • pp.485-495
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    • 2018
  • Background: The geographical origin of Panax ginseng Meyer, a valuable medicinal plant, is important to both ginseng producers and consumers in the context of economic profit and human health benefits. We, therefore, aimed to discriminate between the cultivation regions of ginseng using the stable isotope ratios of C, N, O, and S, which are abundant bioelements in living organisms. Methods: Six Korean ginseng cultivars (3-yr-old roots) were collected from five different regions in Korea. The C, N, O, and S stable isotope ratios in ginseng roots were measured by isotope ratio mass spectrometry, and then these isotope ratio profiles were statistically analyzed using chemometrics. Results: The various isotope ratios found in P. ginseng roots were significantly influenced by region, cultivar, and the interactions between these two factors ($p{\leq}0.001$). The variation in ${\delta}^{15}N$ and ${\delta}^{13}C$ in ginseng roots was significant for discriminating between different ginseng cultivation regions, and ${\delta}^{18}O$ and ${\delta}^{34}S$ were also affected by both altitude and proximity to coastal areas. Chemometric model results tested in this study provided discrimination between the majority of different cultivation regions. Based on the external validation, this chemometric model also showed good model performance ($R^2=0.853$ and $Q^2=0.738$). Conclusion: Our case study elucidates the variation of C, N, O, and S stable isotope ratios in ginseng root depending on cultivation region. Hence, the analysis of stable isotope ratios is a suitable tool for discrimination between the regional origins of ginseng samples from Korea, with potential application to other countries.

The Content of Amino Acids, Free Sugars and Proximate Compositions from Undaria pinnatifida Sporophyll (미역(Undaria pinnatifida)귀의 일반성분, 유리당 함량 및 아미노산 함량)

  • Jin, Seong-Woo;Kim, Kyung-Je;Koh, Young-Woo;Im, Seung-Bin;Ha, Neul-I;Jeong, Hee-Gyeong;Je, Hae-Shin;Ban, Seung-Eon;Jeong, Sang-Wook;Seo, Kyoung-Sun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.77-77
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    • 2018
  • 미역의 뿌리부분인 미역귀에는 알긴산과 후코이단 성분이 풍부하고 고혈압과 당뇨등과 같은 성인병을 예방하는데 도움이 된다. 후코이단은 미역과 다시마 같은 갈조류에서 추출된 천연물질로 특히 면역력을 활성화하고 항암작용으로 잘 알려져 있다. 알긴산 등 유용성분이 함유된 것으로 알려진 미역귀의 유용성분을 분석하여, 향후 미역귀를 활용한 제품개발의 기초자료를 제공하고자 본 연구를 수행하였다. 본 연구에 사용된 미역귀는 2015년 12월 완도 금일수산에서 제공받아 연구재료로 활용하였다. AOAC방법에 따라 분석한 미역귀의 일반성분은 수분이 대부분을 차지하였으며, 조섬유와 가용성 무질소물 함량이 높게 나타났다. 이는 시료로 사용한 미역귀가 수확 후 바로 냉동보관 되어서 수분함량이 높은 것으로 보여진다. 유리당은 미역귀 1g에 증류수를 가하여 $60^{\circ}C$에서 4시간 추출 한 후, 원심분리(3,000 rpm, 30 min)하여 $0.45{\mu}m$ membrane filter (Millipore Co., USA)로 여과한 여액을 HPLC (High Performance Liquid Chromatography)를 이용하여 분석한 결과, 미역귀의 유리당은 Galactose 1종만이 검출되었다. 미역귀의 구성아미노산과 유리아미노산은 $0.45{\mu}m$ membrane filter로 여과하여 얻은 여액을 일정량 취하여 AccQ-Tag 시약을 사용하여 유도체화 시킨 후 HPLC로 분석하였다. 미역귀의 구성아미노산은 glutamic acid, aspartic acid, alanine, lysine, threonine, valine, arginine 순으로 높은 함량을 나타내었으며, 유리아미노산은 glutamic acid, alanine, threonine, arginine, lysine 순으로 높은 함량을 나타내었다. 미역귀의 유리아미노산함량은 표고에 비하여 현저히 낮은 함량이 나타났으며, 주요아미노산은 해조류의 풍미를 대표하는 아미노산인 glutamic acid와 alanine으로 나타났다.

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Performance Analysis of QPSK and QDPSK Signals with Diversity Reception and Coding Techniques in Fading Plus Impulsive Noise Environments (임펄스 잡음과 페이딩이 함께 존재하는 환경에서 다이버시티 수신 기법과 부호화 기법을 채용하는 QPSK 및 QDPSK 신호의 성능 해석)

  • Leem, Kill-Yong;Cho, Sung-Joon;Lee, Jin
    • The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
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    • v.5 no.4
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    • pp.3-17
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    • 1994
  • The error probability of QPSK and DPSK signals with diversity reception technique in m-distribution fading plus impulsive noise environments has been derived and the error probability is evaluated and compared with that in Gaussian noise environment. The error performance degrades as impulsive noise becomes strong and degree of degradation of signal performance in QDPSK signal is larger than that in QPSK signal. The diversity reception technique can improve the error performance not only in fading plus Gaussian noise environment but in fading plus impulsive noise environment. When diversity reception technique is used, the improvement of error performance attains about 10dB to 15dB in terms of CNR as compared with that in non diversity reception. Among diversity techniques the maximal ratio combining is must effective. When diversity reception and coding techniques are used together in impulsive noise plus Rayleigh fading environments, the improvement of error performance attains about 12dB to 15dB in terms of CNR as compared with that of only diversity reception technique case and the improvement of error perform- ance in RS coding attains about 2dB in terms of CNR as compared with that of BCH coding case.

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Secondary Dental Pain and Facial Pain Due to Pansinusitis : A Case Report (범부비동염에 의한 이차성 치통과 안면통: 증례보고)

  • Kim, Nam-Koo;Auh, Q-Schick;Chun, Yang-Hyun;Hong, Jung-Pyo
    • Journal of Oral Medicine and Pain
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    • v.32 no.3
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    • pp.329-336
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    • 2007
  • Toothache is a common complaint in the dental office. Most toothaches have their origin in the pulpal tissues or periodontal structures1). These odontogenic pains are managed well and predictably by dental therapies. One of the most frequent encounters and most confusing phenomena with which the dental diagnostician must deal is the problem of referred pain. The most important step toward proper management of a toothache is to consider that the pain may not be of dental origin. And Patients with orofacial pain, especially those in chronic pain, present a diagnostic and management challenge for the practitioners. There are many structures in the head and neck that can produce heterotopic pains felt in the teeth and other structures. Once referred pain is suspected, the true source of the pain must be located in order to render effective therapy. With increased interest in temporomandibular disorders and orofacial pain, many studies of accurate diagnosis and differential diagnosis about orofacial pain have been established. The purpose of this paper is to present a case for pansinusitis which produced pain referral in teeth and mimicked the symptoms of migraine.

THE COMPARATIVE STUDY ON THE SEDATIVE EFFECT OF ORAL MIDAZOLAM AND INTRAMUSCULAR MIDAZOLAM IN SEDATING YOUNG PEDIATRIC DENIAL PATIENTS (소아 환자에서 미다졸람의 경구 투여와 근육 투여에 의한 진정 효과의 비교 연구)

  • Min, Yu-Jin;Yu, Seung-Hoon;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.1
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    • pp.53-61
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    • 2006
  • When routine behavior control is impossible, midazolam is often used for sedation, because it has wide margin of safety, relatively few side-effects and multiple route of administration. Although intramuscular administration of all administration route is frequently used, it is a major source of anxiety, discomfort, and trauma in children. To the contrary, oral administration of midazolam is easily administered and accepted by children. But, it's therapeutic drug concentration has not been established. The purpose of this study was to compare sedation effect and physiologic parameter of oral midazolam which palliate demerits of intramuscular administration in sedating young pediatric patients with intramuscular midazolam Twelve negative children, mean age 62.5 months, who needed at least two separate restorative visits, requiring local anesthesia participated in this study On every visit, one of the following 2 different sedative regimen was given : (1) 0.30mg/kg midazolam by intramuscular administration (2) 0.75mg/kg by oral administration. Physiologic parameter(oxygen saturation, heart rate) was recorded by ten procedure and behavior was videotaped and rated using Ohio State University Behavior Rating Scale and Automated Counting System by one investigator, blind to administration route The analyzed sedative effect of oral midazolam resulted in good sedative effect, comparing to intramuscular route, And there is no statistically difference between oral and intramuscular administration of midazolam (p>0.05).

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Ecological Changes in Pine Gall Midge-damaged Pinus densiflora Forest at the Southern Temperate Forest Zone in Korea(III) -Analysis of Phytosociological Changes During Seven Years of the Korean Red Pine Community in Cheongwon-gun- (솔잎혹파리 피해적송림(被害赤松林)의 생태학적(生態學的) 연구(硏究)(III) -청원군(淸原郡) 소나무군집(群集)의 7년간(年間)의 식생변화분석(植生變化分析)-)

  • Lee, Kyong Jae;Oh, Koo Kyoon;Yim, Kyong Bin
    • Journal of Korean Society of Forest Science
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    • v.77 no.3
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    • pp.315-321
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    • 1988
  • To investigate and analyze the change in the forest community structure between non-attacked time(in 1980) and severly damaged time(in 1987) by pine gall midge, Thecodoplosis japonensis, four plots with five subplots were sampled at Cheongwon-gun of Chungcheungbuk-do. The total number of woody species per 0.2ha changed 32 in 1980 to 53 in 1987. According to the damage increasing, the importance value of the genus Quercus as a substituting species has sprung up. Especially the importance value of Quercus serrata and Q. aliena singnificantly increased in the heavily insect infested forest. As to changes of forest community structure during 7 years, diversity of investigation structure has increased. The similarity indices between 1980 and 1987 were 52.9% and 49.7% in the understory and shrub stratum, respectively.

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Vegetation Structure of Mountain Ridge from Miwangjae to Cheonwhangbong in Weolchulsan National Park (월출산국립공원 미왕재-천황봉 구간의 능선부 식생구조)

  • Choo Gab-Cheul;Kim Gab-Tae;Cho Hyun-Seo
    • Korean Journal of Environment and Ecology
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    • v.20 no.2
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    • pp.114-121
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    • 2006
  • To investigate the vegetation structure of mountain ridge from Miwangjae to Cheonwhangbong, 11 plots($2000m^2$) set up with random sampling method were surveyed. Three groups of Quercus mongolica community, Quercus mongolica-Styrax japonica community, Q. mongolica-Pinus densiflora community were classified by cluster analysis. Quercus mongolica was a major woody plant species in the ridge area from Miwangjae to Cheonwhangbong, and Styrax japonica and Pinus densiflora was partly occupied. High positive correlations was proved between Prunus sargentii and Euonymus alatus for. ciliato-dentatus; Quercus serrata and Euonymus alatus; Sorbus alnifolia and Euonymus alatus, Magnolia sieboldii; Acer pseudo-sieboldianum and Euonymus alatus for. ciliato-dentatus; Rhododendron schlippenbachii and Euonymus alatus for. ciliato-dentatandus; Euonymus alatus and Lindera obtusiloba, Castanea crenata, Lindera erythrocarpa; Lespedeza bicolor and Rhododendron schlippenbachii; Castanea crenata and Magnolia sieboldii; Magnolia sieboldii and Lindera erythrocarpa, and relatively high negative correlations was proved between Rhododendron schlippenbachii and Euonymus alatus; Lespedeza bicolor and Castanea crenata; Stephanandra incisa and Magnolia sieboldii; Rhododendron yedoense var. poukhanense and Lindera erythrocarpa; Euonymus alatus for. ciliato-dentatus and Magnolia sieboldii; Fraxinus sieboldiana and Sapium japonicum; Prunus sargentii and Lespedeza maximowiczii; Lespedeza maximowiczii and Lindera erythrocarpa. Species diversity(H') of investigated groups was ranged $2.3225{\sim}2.7721$, and it was relatively low value compared to that of mountain ridge area of other national parks.

Growth Responses of 4 Species to NaCl Concentration in Artificial Soil (NaCl 농도별 토양 처리에 대한 4수종의 생장 반응)

  • Park, Woo-Jin;Seo, Byung-Soo;Park, Chong-Min;Choi, Chung-Ho;Choi, Soo-Min
    • Korean Journal of Environment and Ecology
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    • v.24 no.6
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    • pp.735-743
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    • 2010
  • In order to inspect growth responses of Fraxinus rhynchophylla Hance, Koelreuteria paniculata Laxmann, Quercus acutissima Carruther and Ulmus parvifolia Jacquin to NaCl treatment, NaCl solution was treated for four months with 0, 25, 50, 100 and 200 mM concentrations, then survival rate, change of relative growth rate, weight, dry weight and pigment content of leaf were investigated. According to NaCl treatment, pH and EC (electrical conductivity) of soil increased, and growth rates of four tree species fell apparently as treatment time became longer. U. parvifolia had the highest survival rate with 15% in the 200mM treatment, and the other three species withered in the treatment. Relative growth rate, weight and dry weight decreased when NaCl treatment time grew longer. The total chlorophyll declined after it rose to 60 days, and the total chlorophyll and carotenoid of the all species according to NaCl treatment did not change very much. With the result from anlayzing growth responses of four tree species to oxidative stress which occurs during NaCl treatment, U. parvifolia has the highest tolerance, followed by K. paniculata, F. rhynchophylla and Q. acutissima.

Secondary Dispersion of Several Broadleaved Tree Seeds by Wildlife in Mt. Jungwang, Pyeongchang-gun, Korea (중왕산 몇 활엽수 종자의 야생동물에 의한 2차 분산)

  • Kim, Gab-Tae;Kim, Hoi-Jin
    • Korean Journal of Environment and Ecology
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    • v.27 no.1
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    • pp.64-70
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    • 2013
  • To examine the proportion of secondary seed dispersion by wildlife and to identify the related wildlife, We measured seed removal rates and related wildlife at the natural mixed broadleaved forest in mountain Jungwang, Pyeungchang-gun in middle part of Korean peninsula, seed-tray contained four kind of tree seeds were setted from mid-september to late-October for three years. Mean seed removal rates(MSRR) of acorn(Q. mongolica)is shown the highest values 87.2%, next MSRR of samara(A. pseudosieboldianum) does 33.1%, MSRR of samara(A. pictum subsp. mono) does 29.2%. and then SRR of samara(A. mandshuricum) does only 13.6%. Acorns are removed more faster than samaras. Among the related wildlife, Siberian chipmunks most freqently visit seed tray, and ordered Korean squirrel, Wild boar, and Yellow-throated marten. Most secondary dispersed acorns might be consumed by Wild boar, and small portions does comsumed or scatter-hoarded by rodents. Most secondary dispersed samaras might be consumed by rodents, and small portions does scatter-hoarded by rodents. A few sound seeds are remained on the forest floor in late October owing to the seed consunption and seed hoarding by wildlife. We observe seedling establishment of four broadleaved tree species. From these results, We proved Wild boar is seed comsummer, and rodents are seed consummer and seed disperser. Further studies on secondary seed dispersion, seed consumption and hoarding and related wildlife should be needed.

Research on Oral Status of Hearing Impaired Youth by Using QLF-D (QLF-D를 이용한 청각장애 청소년의 구강상태에 관한 조사)

  • Kim, Chang-Suk
    • The Journal of the Korea Contents Association
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    • v.13 no.9
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    • pp.305-311
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    • 2013
  • This study analyzed the oral status after recording the images by using QLF-D with targets of 38 youth people with hearing impairment and hearing language impairment. In order to investigate the state of oral hygiene, plaque index (O'Leary index) and contents of investigation of the state of the teeth included the number of sound teeth, the number of caries teeth, dental caries experience and the number of filling teeth. The following results were obtained. First, women lacked the management on plaque and had more caries teeth compared with men. In terms of impairment classification, subjects with both hearing and language impairment lacked the management on plaque and had more caries teeth. Second, subjects who did not get an oral exam for one year had more caries teeth. Oral hygiene score was the highest with the brushing time for 3-4 minutes. The number of sound teeth was increased as the brushing time was increased. In addition, the oral hygiene management time was the highest when cleaning the teeth, gums and tongue at the same time. Third, it was shown that the satisfaction of oral health education by using the new equipment was high. As a result of this study, in order to improve the oral health level of impaired students, they shall be trained to manage their teeth by themselves and educated to increase their motivation and practice. Thus, it is thought that various approaches which are differentiated from existing methods are required to be tried.