• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.97-106
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• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

• Korean Journal of Applied Biomechanics
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• v.27 no.1
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• pp.9-18
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• 2017

Objective: The purpose of this study was to identify kinematic variables that govern successful performance and judges' scores and to establish correlative relationships among those of Yurchenko layout with a full twist in female vaults. Method: Four video cameras with sampling rate of 60 Hz collected 32 motion data of Yurchenko vaults from twenty-two female participants (age: $18.6{\pm}3.6years$, height: $153.0{\pm}6.5cm$, mass: $44.7{\pm}7.3kg$) during national competition. Posting processing and calculations of kinematic variables were performed in Kwon 3D XP and $Matlab^{(R)}$ programs. Correlation and regression analyses were applied to find the relationships between the obtained scores and kinematic variables. Deterministic model (Hay & Reid, 1988) was used to investigate the strength of correlative relationships among kinematic variables. Results: The obtained scores from the judges' decision were mainly affected by post-flight peak height, horse contact time, knee angle at landing, and horse takeoff angle. Strong blocking during horse contact was required to get successful performance and obtain high scores. Modified deterministic model showed that round-off entrance and takeoff angles and resultant velocity of the center of mass (CM) during the roundoff phase were the starting variables affecting performance in the following kinematics. Knee angle at landing, a highly influential variable on the obtained point, was only determined by judges' decision without significant correlative relationship with previous kinematic variables. Conclusion: The obtained scores highly depended on kinematic variables of post-flight and horse contact phases that were affected by those from the previous phases including round-off postures and resultant velocity of the body center of mass.

• Journal of Welding and Joining
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• v.28 no.2
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• pp.84-90
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• 2010

Fillet welding accounts for about 80% of all constructing process of ship and ocean structure. T-joint is one of the typical shapes which are frequently reported to experience the fatigue damage when the marine structure meets the storm loads. The fatigue damage is affected by the magnitude of residual stresses on the weld. Recently, many shipping registers and design guidances have required that the fatigue strength assessment method should be compensated by the effect of the residual stress in case that the random loading or storm loading is applied to the marine vessels. This study suggests the computational procedure to analyze the residual stresses of T-joint specimen that is frequently reported to get damaged by the storm loading. Experiment by XRD as well as the 3-D computational welding model is presented in order to get the profile of residual stress. Throughout the comparison of experimental result with the computational result, the computational model was validated. Thereafter, characteristics of he residual stresses in the joint are discussed.

• Korean Journal of Materials Research
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• v.23 no.12
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• pp.732-736
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• 2013

AlN epilayers were grown on a c-plane sapphire substrate using hydride vapor phase epitaxy (HVPE). A series of AlN epilayers were grown at $1120^{\circ}C$ with V/III ratios 1.5, 2.5 and 3.5, and the influence of V/III ratio on their properties was investigated. As the V/III ratio was increased, the surface roughness (RMS roughness), Raman shift of $E_2$ high peaks and full-width at half-maximum (FWHM) of symmetrical (002) & asymmetrical (102) of the AlN epilayers increased. However, the intensities of the Raman $E_2$ high peaks were reduced. This indicates that the crystal quality of the grown AlN epilayers was degraded by activation of the parasitic reaction as the V/III ratio was increased. Smooth surface, stress free and high crystal quality AlN epilayers were obtained at the V/III ratio of 1.5. The crystal quality of AlNepilayers is worsened by the promotion of three-dimensional (3D) growth mode when the flow of $NH_3$ is high.

• Water for future
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• v.27 no.1
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• pp.79-88
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• 1994

This study was conducted to analyse groundwater flow in the Bugok hot spring area using the MODFLOW model which can simulate three dimensional groundwater flow both in confined and unconfined aquifers. Based on this study the following conclusions were obtained: 1) The hydraulic conductivity and the specific storage of the aquifer were 0.0135 m/day and 0.020, respectively, and the model-predicted groundwater elevation agreed well with the observed one. 2) Simulation results showed that the groundwater level declines at the end of the one-year simulation period when the annual recharge rate is small and the annual pumping rate high, which is the worst combination. Except that combination, the groundwater level does not decline at the end of one-year simulation period indication the pumping rates used were allowable. 3) The safe yield depends upon the magnitudes of the recharge and pumping rates. The pumping rate should not produce excess decline of groundwater level around April when the water level is the lowest in a year.

• Applied Microscopy
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• v.48 no.3
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• pp.55-61
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• 2018

The synaptic vesicle is a specialized structure in presynaptic terminals that stores various neurotransmitters. The actin filament has been proposed for playing an important role in mobilizing synaptic vesicles. To understand the role of actin filament on synaptic vesicle pooling, we characterized synaptic vesicles and actin filament after treatment of brain-derived neurotrophic factor (BDNF) or Latrunculin A on primary cultured neuron from rat embryo hippocampus. Western blots revealed that BDNF treatment increased the expression of synapsin I protein, but Latrunculin A treatment decreased the synapsin I protein expression. The increased expression of synapsin I after BDNF disappeared by the treatment of Latrunculin A. Three-dimensional (3D) tomography of synapse showed that more synaptic vesicles localized near the active zone and total number of synaptic vesicles increased after treatment of BDNF. But the number of synaptic vesicle was 2.5-fold reduced in presynaptic terminals and the loss of filamentous network was observed after Latrunculin A application. The treatment of Latruculin A after preincubation of BDNF group showed that synaptic vesicle number was similar to that of control group, but filamentous structures were not restored. These data suggest that the actin filament plays a significant role in synaptic vesicles pooling in presynaptic terminals.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.1
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• pp.43-52
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• 2009

LIDAR data, as the source of the 3D information of buildings, are used many modeling fields such as three-dimensional city models in urban planning and the visibility analysis of buildings. This study suggests a methodology, that is characterized by combining image-based and point-based process, for minimizing the user's intervention and automatically extracting building boundary only using the LIDAR data. Image processing methodology is firstly used to separate building and non-building regions from LIDAR data. Moreover, building regions are then classified main roof into remaining parts by the statistical analysis of height values, and the remaining parts are processed separately. Through the experimental results of study areas which exist many types of buildings, for example, apartment-type, stair-type, complex-type, etc. Approximately 90% building boundaries are automatically extracted by the proposed methodology.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.5
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• pp.395-404
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• 2012

This paper presents computations of the dynamic derivatives of three dimensional flight vehicle configurations using CFD. The pitch dynamic derivatives are computed from the pitch sinusoidal motion, while the roll damping is computed based on steady state calculation using a non-inertial frame method. The Basic Finner and the SDM(Standard Dynamic Model) are chosen for the benchmark tests against other numerical and experimental results. For the flow calculations, a 3-D Euler solver that can be run both on the non-inertial frame and on the inertial frame is developed. A dual-time stepping method is applied for the unsteady time accurate simulations. A good agreement of pitch-roll dynamic derivatives with previously published numerical results and the experimental results is observed.

• Journal of Embryo Transfer
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• v.25 no.4
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• pp.281-286
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• 2010

This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was $300\;{\mu}l$. Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.

• Physical Therapy Korea
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• v.27 no.3
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• pp.199-205
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• 2020

Background: The classification of foot type can be commonly determined by the height of the media longitudinal arch. The normalized arch height (NAH) is defined as the ratio of navicular or instep heights to the foot length or instep length. Objects: This study investigated the relationships among foot characteristics, such as foot length (FL), instep length (IL), navicular height (NH), and instep height (IH), in Korean young adults. Also, the distribution of foot type based on calculated NAH was assessed. Methods: Three-dimensional foot scanning data of young adults aged 20 to 39 years (total: 1,978; 974 male, 1,004 female) were obtained from the Korea Technology Standards Institute, and used for analyses. NAH was calculated as the following: NH/FL, IH/FL, IH/IL, NH/IL. Spearman's rank order correlation was used to identify correlations among variables. The Mann-Whitney U-test and chi-square test were used to compare the sex differences in foot characteristics and distribution of foot type. Results: FL and IL showed a very high correlation (r = 0.94). The correlations between FL or IL and IH (r = 0.50-0.57) were greater than those between FL or IL and NH (r = 0.23-0.72). Males had significantly larger values than females (p < 0.001), and the frequency of pes planus was significantly higher in females than in males (χ² = 50.09, p < 0.001). Based on the IH/IL index, the neutral foot, pes planus and pes cavus distributed by 16%, 78%, and 6% respectively. Conclusion: Our results on foot arch distribution could be used as basic data in clinical or footwear fields, and our data on differences in arch structure according to sex may facilitate understanding of why injury to the lower limbs differs between males and females.