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Effects of Dietary Vitamins C and E on Egg Shell Quality of Broiler Breeder Hens Exposed to Heat Stress

  • Chung, M.K.;Choi, J.H.;Chung, Y.K.;Chee, K.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.4
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    • pp.545-551
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    • 2005
  • A feeding trial was conducted to determine whether dietary vitamin C (200 mg/kg) and vitamin E (250 mg/kg) prevent any drops in egg shell quality under heat stress in broiler breeder hens. One hundred and sixty molted Ross broiler breeders were housed randomly in an individual cage at 83 weeks of age. Four dietary treatments with forty hens and four replications per treatment were control (no additional vitamins), vitamin C-, or vitamin E-supplemented and combined supplementation of the two vitamins. After a tenday-adaptation period at 25$^{\circ}C$, the ambient temperature was kept at 32$^{\circ}C$ for a three-week-testing period. Egg production dropped dramatically over week but it did not show a significant change among treatments (p<0.05). However, egg quality parameters such as egg weight, specific gravity, shell thickness, SWUSA, puncture force and shell breaking strength from the birds fed the diet with the combined vitamins C and E were significantly improved over those of the control group during the heat stress period (p<0.05). The hens fed the vitamin C diet improved tibia breaking strength (37.16 kg), statistically higher than the birds fed the control and the vitamin E diets (p<0.05). The hens fed the control diet showed higher serum corticosterone levels, a mean of 5.97 ng/ml, than those of the other treatments (p<0.05). The heat stress resulted in elevated heterophils and decreased lymphocytes in serum, increasing the H/L ratios for all the treatments. However, the increases in H/L ratios were alleviated by feeding the diets containing vitamin C alone or together with vitamin E, although there were no significant differences in the ratio between the two groups (p<0.05). In conclusion, vitamins C (200 mg/kg) and/or E (250 mg/kg) supplemented to the diets for broiler breeder hens could prevent drops in egg shell quality and tibia bone strength under highly stressful environmental temperatures.

Effects of Dietary Lycopene and Vitamin E on Egg Production, Antioxidant Status and Cholesterol Levels in Japanese Quail

  • Sahin, N.;Sahin, K.;Onderci, M.;Karatepe, M.;Smith, M.O.;Kucuk, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.224-230
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    • 2006
  • Japanese Quails were used to evaluate the effects of dietary supplementation with vitamin E (dl-a-tocopheryl-acetate), lycopene, and their combination on egg production, egg quality, concentrations of malondialdehyde (MDA), vitamin E, A and cholesterol in serum and egg yolk. Quails (n = 120; 55 d old) were divided into four groups (n = 30/group) and fed a basal diet or the basal diet supplemented with lycopene (100 mg/kg diet), vitamin E (250 mg dl-${\alpha}$-tocopheryl-acetate/kg diet) or a combination of lycopene and vitamin E (100 mg/kg lycopene plus 250 mg dl-${\alpha}$-tocopheryl-acetate/kg diet). Vitamin E and lycopene did not affect (p>0.05) body weight, feed intake or egg weight. Egg production and Haugh unit were greater (p<0.05) in each supplemental group compared with the control group (p<0.05). Serum and liver MDA levels were decreased in supplemented groups compared with the control group. Separately or as a combination, supplemental lycopene and vitamin E increased serum and egg yolk vitamin E and A but decreased cholesterol concentrations (p<0.05). In general, when a significant effect was found for a parameter, the magnitude of the responses to vitamin and lycopene supplements was greatest with the combination of the lycopene and vitamin E, rather than that observed with each supplement separately. Results of the present study indicate that supplementing with a combination of dietary lycopene and vitamin E reduced serum and yolk cholesterol concentrations and improved antioxidant status.

Physical and functional properties of tunicate (Styela clava) hydrolysate obtained from pressurized hydrothermal process

  • Lee, Hee-Jeong;Chae, Sol-Ji;Saravana, Periaswamy Sivagnanam;Chun, Byung-Soo
    • Fisheries and Aquatic Sciences
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    • v.20 no.7
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    • pp.14.1-14.8
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    • 2017
  • In this study, marine tunicate Styela clava hydrolysate was produced by an environment friendly and green technology, pressurized hot water hydrolysis (PHWH) at different temperatures ($125-275^{\circ}C$) and pressure 50 bar. A wide range of physico-chemical and bio-functional properties such as color, pH, protein content, total carbohydrate content, reducing sugar content, and radical scavenging activities of the produced hydrolysates were evaluated. The appearance (color) of hydrolysates varied depending on the temperature; hydrolysates obtained at $125-150^{\circ}C$ were lighter, whereas at $175^{\circ}C$ gave reddish-yellow, and $225^{\circ}C$ gave dark brown hydrolysates. The $L^*$ (lightness), $a^*$ (red-green), and $b^*$ (yellow-blue) values of the hydrolysates varied between 35.20 and 50.21, -0.28 and 9.59, and 6.45 and 28.82, respectively. The pH values of S. clava hydrolysates varied from 6.45 ($125^{\circ}C$) to 8.96 ($275^{\circ}C$) and the values were found to be increased as the temperature was increased. The hydrolysis efficiency of S. clava hydrolysate was ranged from 46.05 to 88.67% and the highest value was found at $250^{\circ}C$. The highest protein, total carbohydrate content, and reducing sugar content of the hydrolysates were found 4.52 mg/g bovine, 11.48 mg/g and 2.77 mg/g at 175, and 200 and $200^{\circ}C$, respectively. Hydrolysates obtained at lower temperature showed poor radical scavenging activity and the highest DPPH, ABTS, and FRAP activities were obtained 10.25, 14.06, and 10.91 mg trolox equivalent/g hydrolysate (dry matter basis), respectively. Therefore, S. clava hydrolysate obtained by PHWH at $225-250^{\circ}C$ and 50 bar is recommended for bio-functional food supplement preparation.

Syntheses of the derivatives of chitin and chitosan, and their physicochemical properties (키틴 및 키토산 유도체의 합성과 그 물리화학적 특성)

  • Byun, Hee-Guk;Kang, Ok-Ju;Kim, Se-Kwon
    • Applied Biological Chemistry
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    • v.35 no.4
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    • pp.265-271
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    • 1992
  • Derivatives (microcrystalline chitin, carboxymethylchitin, acetylchitin, N-acetylchitosan, ethylchitosan and chitosansulfate) of chitin and chitosan were synthesized, and the physicochemical properties of the derivatives were compared with those of chitin and chitosan. Carboxymethylchitin was soluble in water or acetic acid, whereas chitosan and ethylchitosan were soluble in acetic acid alone. The water binding capacity of N-acetylchitosan was two fold higher than that of chitin. Lipid binding capacity of carboxymethylchitin was the highest, holding 1800%, and that of chitin was the lowest, holding 511% among the derivatives. Carboxymethylchitin among the derivatives showed the highest emulsifying capacity, however chitin and chitosan didn't produce emulsions. Dye binding capacity of acetylchitin was the highest, holding 0.93 mg dye/g sample (Blue R-250) and 0.96 mg dye/g sample (Red No. 2). It was concluded that carboxymethylchitin is a good emulsifier and N-acetylchitosan, chitosansulfate and chitosan are suitable for use as dye absorbents.

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Solid-Liquid Separation Characteristics with Bio-filter Media Reactor (여과분리형 생물반응조의 고액분리 특성)

  • Park, Young Bae;Jung, Yong Jun
    • Journal of Wetlands Research
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    • v.16 no.3
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    • pp.347-353
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    • 2014
  • This work was performed to find the effect of operation parameters on the permeate flux through the activated sludge dynamic layer, and to indicate the relationship between the water quality of supernatant and flux based on the results. Since the effluent can be obtained through steady and stable formation of cake layer in the bio-filter media system, it is an important subject to keep and control microbes with activated state in the bio-reactor. Filtration resistance was drastically increased at more than 18000mg/L of MLSS. With filtration time continued, the flux was gradually decreased and the water qualities of supernatant monitored by turbidity and TOC were also deteriorated. This phenomenon indicated that the organic materials generated by microbes and accumulated in the reactor might affect the flux in the system. In addition, the decrease of flux was simultaneously observed in the sludge volume index. When SVI was controlled from 150 to 250, the flux was also decreased. The proper aeration time was recommended to 30 to 60 seconds in this system. In order to operate this system steadily, therefore, the control of water quality of supernatant and SVI should be proceeded.

Coulometric Determination of Plutonium in PWR Spent Fuels (PWR 사용후핵연료내 플루토늄의 전기량적 정량)

  • Sohn, Se Chul;Suh, Moo Yul;Kim, Jung Suk;Song, Byung Chul;Jee, Kwang Yong;Choi,In Kyu;Kim, Won Ho
    • Journal of the Korean Chemical Society
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    • v.44 no.6
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    • pp.581-586
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    • 2000
  • Separation and coulometric titration method were applied for the determination of plutonium content in samples of PWR spent fuel. Plutonium was separated on an anion exchange(AG MP-1) column and determined by the controlled-potential coulometric titration. In this study, we discussed some experimental conditions related to the separation and determination of plutonium in PWR spent fuel samples. Average accuracy(recovery of plutonium) for the determination of 0.230∼3.02 mg plutonium standard was 99.36%. Average precision(relative standard deviation, RSD) for the determination of 0.250∼0.450 mg plutonium in PWR spent fuel samples was 0.38%.

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Copper Toxicity on Survival, Respiration and Organ Structure of Tegillarca granosa (Bivalvia: Arcidae) (꼬막, Tegillarca granosa의 생존, 호흡 및 기관계 구조에 미치는 구리 (Cu) 의 독성)

  • Shin, Yun Kyung;Park, Jung Jun;Ju, Sun Mi;Lee, Jung Sick
    • The Korean Journal of Malacology
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    • v.31 no.2
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    • pp.151-158
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    • 2015
  • This study was conducted to find out the changes of survival, respiration and organ structure of Tegillarca granosa exposed to copper (Cu). Experimental period was four weeks. Experimental groups were composed of one control condition and three copper exposure conditions (0.125, 0.250 and 0.500 mg/L). The results of the study confirmed that copper induces reduction of survival rate and respiration rate and histopathology of organ structure of the bivalve. In the copper concentration of 0.500 mg/L, mortality was 66.7% after Cu exposure of 4 weeks. Respiration rate was observed exposure groups lower than control decline by 18%. Histological analysis of organ system illustrated degeneration of epithelial layer and connective tissue layer of the mantle. Also, histological degenerations as epithelial atrophy and disappearance of lateral cilia are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, acidification of mucous and degeneration of muscle fiber bundles in the foot. In the digestive diverticulum, it was showed atrophy and destruction of basophilic cell and epithelial cell in the digestive tubules.

The study of ferroelectric properties for Pb(Mg1/3Ta2/3)O3-PbTiO3 solid solution modified with ZrO2 (ZrO2 첨가에 따른 Pb(Mg1/3Ta2/3)O3-PbTiO3 고용체의 강유전 특성 연구)

  • Kim, B.G.
    • Journal of the Korean Vacuum Society
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    • v.17 no.4
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    • pp.317-321
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    • 2008
  • The $0.65Pb(Mg_{1/3}Ta_{2/3})O_3-0.35PbTiO_3$ (PMT-PT)ceramics were near morphotropic phase boundary. The dielectric constant, the loss, and pyroelectric coefficient of the ceramics were measured as a function of temperature ($25^{\circ}C\;{\sim}\;250^{\circ}C$). The dielectric constant, the loss, and the pyroelectric coefficient could be improved with the addition of small amounts of $ZrO_2$ up to 0.2 mol%. The dielectric and pyroelectric peak temperature are slightly shifted to lower temperatrues with the addition of amount of $ZrO_2$.

Analysis of structural properties of epitaxial BST thin films prepared by pulsed laser deposition (펄스형 레이저 증착법으로 제조된 에피탁시 BST 박막의 구조 분석)

  • 김상섭;제정호
    • Journal of the Korean Vacuum Society
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    • v.7 no.4
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    • pp.355-360
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    • 1998
  • Epitaxial $Ba_{0.5}Sr_{0.5}TiO_3$thin films of two different thickness (~250 $\AA$ and ~1340 $\AA$) on MgO(001) prepared by a pulsed laser deposition method were studied by synchroton x-ray scattering measurements. The film initially grew on MgO(001) with a cube-on-cube relationship, maintaining it during further growth. As the film grew, the surface of the film became rough significantly, but the interface between the film and the substrate seemed to have changed little. In the early stage, the film was highly strained in a tetragonal structure with the longer axis parallel to the surface normal direction. As the growth proceeded further, it was mostly relaxed to a cubic structure with the lattice parameter of the bulk value and the mosaic distribution improved significantly in both in-plane and out-of-plane directions.

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Effect of Rhus chinensis Gall Extract on Liver Function, Plasma Lipid Composition and Antioxidant System in Rats with High Fat Diet (오배자 추출물이 고지방식이를 급여한 흰쥐의 간기능, 혈청지질구성 및 항산화계에 미치는 영향)

  • Choi, Moo-Young;Choi, Eun-Jung;Lee, Eun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.3
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    • pp.632-637
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    • 1999
  • The purposes of this study is to investigate the effect of Rhus chinensis gall extract on liver function, plasma lipid composition and antioxidant system in the obese rats with high fat diet for seven weeks. Thirty two male rats of Sprague Dawely strain were divided into four groups. they are normal group (basal diet), control group(high fat diet), III group(high fat diet with Rhus chinensis gall extract 25mg/kg body weight per day) and IV group(high fat diet with Rhus chinensis gall extract 250mg/kg body weight per day). Rats in III group and IV group were administered with Rhus chinensis gall extract accordingly. Weight gains showed a tendency to decrease in rat with Rhus chinensis gall extract group but showed no differences among treatment groups. Food intake and Food efficiency ratio were not significantly different among treatment groups. Plasma total cholesterol showed a tendency to decrease in Rhus chinensis gall extract group. HDL cholesterol showed a tendency to decrease in 25mg/kg of Rhus chinensis gall extract group. However, in the 250mg/kg of Rhus chinensis gall extract group, these values showed no significant difference compared to the control group (p<0.05). GPT activities showed no significant difference among treatment groups. GOT activities showed a tendency to decrease in the groups of Rhus chinensis gall extract groups. Lipid peroxide level was significantly higher in control group than those of normal group. In Rhus chinensis gall extract groups, lipid peroxide level showed a tendency to decrease, but glutathione peroxidase activity was progressively increased. Results of our research in this paper show that Rhus chinensis gall extract might improve liver function, antioxidant system and plasma lipid composition in rats with high fat diet.

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