The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Copper Toxicity on Survival, Respiration and Organ Structure of Tegillarca granosa (Bivalvia: Arcidae)

꼬막, Tegillarca granosa의 생존, 호흡 및 기관계 구조에 미치는 구리 (Cu) 의 독성

  • Shin, Yun Kyung (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Park, Jung Jun (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Ju, Sun Mi (Department of Aqualife Medicine, Chonnam National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
  • 신윤경 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 박정준 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 주선미 (전남대학교 수산생명의학과) ;
  • 이정식 (전남대학교 수산생명의학과)
  • Received : 2015.06.09
  • Accepted : 2015.06.29
  • Published : 2015.06.30
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Abstract

This study was conducted to find out the changes of survival, respiration and organ structure of Tegillarca granosa exposed to copper (Cu). Experimental period was four weeks. Experimental groups were composed of one control condition and three copper exposure conditions (0.125, 0.250 and 0.500 mg/L). The results of the study confirmed that copper induces reduction of survival rate and respiration rate and histopathology of organ structure of the bivalve. In the copper concentration of 0.500 mg/L, mortality was 66.7% after Cu exposure of 4 weeks. Respiration rate was observed exposure groups lower than control decline by 18%. Histological analysis of organ system illustrated degeneration of epithelial layer and connective tissue layer of the mantle. Also, histological degenerations as epithelial atrophy and disappearance of lateral cilia are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, acidification of mucous and degeneration of muscle fiber bundles in the foot. In the digestive diverticulum, it was showed atrophy and destruction of basophilic cell and epithelial cell in the digestive tubules.

본 연구는 구리에 노출된 꼬막, Tegillarca granosa의 생존율, 호흡율 및 기관계의 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구와 구리 노출구 3개 (0.125, 0.250 and 0.500 mg/L) 였다. 실험 결과 구리는 꼬막의 생존율과 호흡률의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 노출 종말점에서 0.500 mg/L의 구리 노출구에서 사망률은 66.7%였으며, 호흡률은 대조구에 비해 18.2%로 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 새엽 상피세포의 위축과 측면섬모의 소실, 발에서는 상피층의 붕괴, 점액의 산성화 및 혈림프동의 확장과 근섬유 다발의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성세포와 상피세포의 위축 및 붕괴가 확인되었다.

Keywords

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