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Nematicidal Effect of Root-knot Nematode (Meloidogyne incognita) by Biological Nematicide (생물학적 선충 방제제를 이용한 고구마 뿌리혹선충 (Meloidogyne incognita)의 방제효과)

  • Park, Moon-Hyun;Kim, Jin-Kwang;Choi, Won-Ho;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.2
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    • pp.228-235
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    • 2011
  • An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Pseudomonas putida C-5, which degrade the collagen and gelatin, was isolated from controlled horticultural soils in Seonnam-myun, Sungju-gun, Kyungpook and Woosung-myun, Gongju-shi, Chungnam to develop biological nematode pesticide. When $5,000mL\;L^{-1}$ of A. thaumasia Nema-1 culture was treated to Meloidogyne incognita, the nematicidal activity resulted in 55% at 72 hours after treatment. While the nematicidal activity increased to 65% by treating the culture mixture of $5,000mL\;L^{-1}$ Nema-1 and P. putida C-5 after 72 hours. Furthermore, the nematicidal activity of the mixture containing cinnamon extract $50mg\;L^{-1}$, each $5,000mL\;L^{-1}$ of Nema-1 and C-5 culture was elevated to 89% at 72 hours after treatment, comparing to the result showed 17% and 57% of the nematicidal activity, respectively by the treatment of chemical nemato pesticide Fosthiazate $50mg\;L^{-1}$ and neem oil $2,000mL\;L^{-1}$. These results suggested that the mixture of microorganisms and plant extract were more effective biological nematicide than the case of only microorganism or plant extract for nematode control.

Tryptic Digestion and Cytochalasin B Binding Assay of the Human HepG2-Type Glucose Transporter Expressed in Spodoptera frugiperda Clone 21-AE Cells

  • Lee Chong-Kee
    • Biomedical Science Letters
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    • v.11 no.1
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    • pp.57-61
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    • 2005
  • The number of sites at which a protein can be readily cleaved by a proteolytic enzyme is greatly influenced by its three-dimensional structure. For native, properly-folded proteins both the rate of cleavage and number of sites at which cleavage takes place are usually much less than for the denatured protein. In order to compare the tertiary structure of recombinant HepG2 type glucose transporter with that of its native counterpart in the erythrocyte, the pattern of tryptic cleavage of the protein expressed in insect cell membranes was therefore examined. After 30 minutes digestion, a fragment of approximate Mr 19,000-21,000 was generated. In addition to this, there were two less intensely stained fragments of apparent Mr 28,000 and 17,000. The pattern of labelling was similar up to 2 hours of digestion. However, the fragments of Mr 19,000-21,000 and Mr 17,000 were no longer detectable after 4 hours digestion. The observation of a very similar pattern of fragments yielded by tryptic digestion of the HepG2 type transporter expressed in insect cells suggests that the recombinant protein exhibits a tertiary structure similar if not identical to that of its human counterpart. Also, the endogenous sugar transporter(s) present in Sf21 cells did not bind cytochalasin B, the potent transporter inhibitor. Therefore, the baculovirus/Spodoptera frugiperda (Sf) cell expression system could be very useful for production of large amounts of human glucose transporters, heterologously.

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Studies on the Antioxidant Effect of Korean Propolis (국산 프로폴리스의 합산화 효과에 관한 연구)

  • 김희재;황보식;이수원
    • Food Science of Animal Resources
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    • v.22 no.1
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    • pp.77-80
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    • 2002
  • The studies was conducted to investigate the antioxidation effect of Korean propolis. Oxidation period of soy oil was 17.5 hr, soy oil with propolis(1,000 ppm) was extended the length of oxidation period. The length of oxidation periods of soy oil with Yecheon, Youngwol, Brazilian, Chinese, and Australian propolis were 33.5, 35.4, 32.0, 33.1, and 27.1hours respectively. The length of oxidation period of lard with propolis 1,000ppm of Yecheon, Youngwol, Brazilian, Chinese and Australian were 191, 167, 296, 230, and 207hours, respectively compared to control(22.5hours). When compared to tocopherol as natural antioxidant and BHT as synthetic antioxidant were revealed 16.7 and 20.5hours, respectively. The length of oxidation period was not different when compared to tocopherol and Youngwol propolis with soy oil. Ascorbic acid and citric acid had synergistic effect to propolis with soy oil when 200ppm of propolis was added.

A study of lifetime prediction of PV module using damp heat test (고온고습 시험을 이용한 실리콘 태양전지 모듈의 수명 예측 연구)

  • Oh, Won Wook;Kang, Byung Jun;Park, Nochang;Tark, Sung Ju;Kim, Young Do;Kim, Donghwan
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.63.1-63.1
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    • 2011
  • To analyze the phenomenon of corrosion in the PV module, we experimented damp heat test at $85^{\circ}C$/85% relative humidity(RH) and $65^{\circ}C$/85% RH for 2,000 hours, respectively. We used 30 mini-modules designed of 6inch one cell. Despite of 2,000 hours test, measured $P_{max}$ is not reached failure which is defined less than 80% compared to initial $P_{max}$. Therefore, we calculate proper curve fitting over 2,000 hours. Data less than 80% $P_{max}$ is found and B10 lifetime is calculated by the number of failure specimens and weibull distribution. Using B10 lifetime that the point of failure rate 10% and Peck's model, the predictable equation of lifetime was derived under temperature and humidity condition.

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University Residence Hall Foodservice in Korea : Investigation on the Operation and Management Systems (전국 대학 기숙사 급식소의 운영 현황 및 실태 조사)

  • Yang, Il-Sun;Weon, Chi-Hyun;Kang, Hye-Seung
    • Journal of the Korean Society of Food Culture
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    • v.15 no.2
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    • pp.69-78
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    • 2000
  • The purposes of this study were to : (a) investigate overall operating conditions of university residence hall foodservice, (b) compare them by the management systems. Questionnaires were hand delivered and mailed to 73 foodservice managers. A total of 56 were usable; resulting in 76.7% response rate. The survey was conducted between October, 1998 and May, 1999. Statistical data analysis was completed using the SAS/Win 6.12 for Descriptive Analysis, $x^2-test$. Sixty-four percent of the foodservice operations were 'self-operated'; 23% were 'contracted'; and 13% were 'rented'. Eighty-three percent of operations provided 'single menu'; forty-six percent of foodservice operations with 'partially self-service'; and forty-one percent with 'complete self-service'. Forty-four percent of overall foodservice operations provided 1,000-2,000 meals per day; thirty-one percent provided less than 1,000 meals per day, and twenty-four percent provided more than 2,000 meals per day. The highest meal price was 1,950 won and the lowest was 700 won with average of 1,295 won. The mean number of employees was 12.2; the number of working hours was 59.7 hours per week.

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The Determinants of Health Promoting Behavior of Industrial Workers (산업장 근로자의 건강증진행위와 자아개념 및 건강의 중요성 인식에 관한 연구)

  • Kim, Chung Nam
    • Korean Journal of Occupational Health Nursing
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    • v.7 no.1
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    • pp.5-19
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    • 1998
  • This descriptive-correlational study was conducted to identify the major factors affecting health promoting behaviors. 344 workers who employed in four different manutacturing plants in Taegu and Kyungbuk area were selected by convenience sampling method. Data were collected from April let to April 18th, 1998 by ready structured questionaires. The purpose of this study was to offer the basic data for health promotion theory development and health promotion strategy planning. This study was based on Pender's Health Promotion Model and examined three variables health promoting behavior, self-concept and perceived importance of health. The Life Style and Health Habit Assessment scale(LHHA) developed by Pender(1982).The Self-concept scale developed by Choi(1972) and the Health Value scale developed by Wallston, Maides and Wallston(1980) were used for this study. Data was analyzed by percentage, mean. t-test. ANOVA, Pearson Correlation Coefficient, and Stepwise Multiple Regression. The major findings of this study are as follows ; 1. The average level of health promoting behavior practice was 63.2% and possible range was from 62 to 248 point. The mean score of respondent's positive self-concept was 75.8. 81.4% of respondents put a high priority on the importance of health. 2. There was a significant difference between the practice level in the category of general self care and less amount of working hours per day(P=0.000), less amount of working hours per week(P=0.000). There was a significant difference between the practice level in the category of nutrition and age(0.002), marital status(0.000), working hour per day(0.008), working hours per week(0.001), There was a significant difference between the practice level in the category of nutriton and sex(0.000), age(0.000), marital status(0.025), education level(0.000), working hours per day(0.002), working hours per week(0.006). There was a significant difference between the practice level in the category of sleep and rest and age(0.003), marital status(0.002), working hours per day(0.001), working hours per week(0.001). There was a significant difference between the practice level in the category of stress management and working hours per day(0.001), working hours per week(0.002). There was a significant difference between the practice level in the category of self-actualization and working hours per day(0.050). 3. General characteristics influencing the respodent's self-concept were level(P=0.009) and worksite(P=0.001). 4. The results of the hypothesis tests are as follows The first hypothesis, that "The respondent who have more positive self-concept will have higher scores in the practice of health promoting behavior." was supported(r=0.2973, P=0.0001). The second hypothesis that "The respondent who have higher perception level on importance of health will have higher scores in the practice health promoting behavior." was rejected(r=- 0665, P=0.2225). 5. The most important factor that affects health promoting behavior practice was working hours per week(6.0%). The combination of working hours per week, age, education level accounted for 10.0% of the variance in health promoting behavior. In conclusion, the results of this study on industrial workers supported Pender's health promotion model in partial and showed the relatedness between self concept and the practice of health promoting behavior. Further research is required to find factors influencing health promoting behaviors of industrial workers.

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Studies on the Antibiotic Residues in Milk of Cows, Goats and Dogs (유우(乳牛), 산양(山羊) 및 견(犬)의 유즙내(乳汁內) 잔류항생물질(殘留抗生物質)에 관한 연구(硏究))

  • Kim, Kyo Jun
    • Korean Journal of Agricultural Science
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    • v.2 no.1
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    • pp.199-231
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    • 1975
  • It is well known fact that antibiotic residues in milk of cows create significant problem for the fermented dairy industry and public health because of inhibition of starter activity and of creation of allergic disease. It can be assumed that antibiotic residues in milk of other aniimals also can create some problems for their infants as in the case of humen. For the above mentioned reasons, present studies were undertaken to determine concentration and duration of antibiotic residues in milk of cows, goats and dogs following intramuscular or intravenous injection and intramammary infusion of penicillin, streptomycin and oxytetracycline at usual dosage. The cylinder-plate method was used for their assay. The results obtained were summerized as follows: 1) Following the intramuscular injection of penicillin, the antibiotic was detected in milk of cows up to 72 hours, in milk of goats 48 hours and in milk of dogs 60 hours of postinjection. The mean peak concentrations were recorded at 12 hours as 0.136 I.U./ml in cows. 6 hours as 0.773 I.U./ml in goats and 3 hours as 1.192 I.U./ml in dogs. 2) Following the intramuscular injection of streptomycin, the antibiotic was detected in milk of cows and goats up to 36 hours and in milk of dogs 24 hours of post-injection. The mean peak concentration were recorded at 6 hours as $0.26{\mu}g/ml$ in cows and at 3 hours in goats and dogs $0.45{\mu}g/ml$ and $0.36{\mu}g/ml$ respectively. 3) Following the intra venous injection of oxytetracycline, the antibiotic was detectable in milk of all the test animals up to 48 hours of postinjection. The mean peak concentrations were recorded at 6 hours as $3.5{\mu}g/ml$ in cows $2.4{\mu}g/ml$ in goats and $2.0{\mu}g/ml$ in dogs respectively. 4) Following intrarnammary infusion of penicillin in amounts of 100,000 I.U. for cows, 20.000 I.U. for goats and 10,000 I.U. for dogs, the penicillin residues in milk of the infused quarter perssisted to 72 hours in cows and 84 hours in goats and dogs. 5) Following intramammary infusion of streptomycin in amount of 500mg for cows, 100mg for goats and 25mg for dogs, the streptomycin residues in milk of the infused quarter persisted to 72 hours in cows and goats and 60 hours in dogs. 6) Following intramammary infusion of oxytetracycline in amount of 500mg for cows, 100mg for goats and 25mg for dogs, the oxytetracycline residues in milk of the infused quarter persisted to 72 hours in cows and 60 hours in goats and dogs. 7) A corelation between the residual antibiotic concentration and milk yield in cows and goats was observed; That is, the lower in the milk production showed a higher the concentration of an antibiotic residues and a longer the time in persistance. 8) Intramammary transfer of the antibiotic from an infused to non infused quarters, in dogs, was observed following the intramammary infusion of penicillin. streptomycin and oxytetracyclne in amounts of 10.000 I.U. 25mg and 25mg respectively. However, no transfer by 100.000 I.U. or 20.000 I.U. of penicillin. 500mg of streptomycin and 100mg of oxytetracyline was observed in cows and goats. 9) In dogs, minimum dosage of antibiotics for transfer fro in treated to untreated quarters following intramammary infusion were 2,500 I.U. of penicillin and 5mg each of streptomycin and oxytetracycline.

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The Study on the Effects of Hospice Care on the Pain Management of the Terminal Cancer Patients (호스피스간호가 말기암환자의 통증에 미치는 효과에 관한 연구)

  • Yoon, Mae-Ok
    • Journal of Hospice and Palliative Care
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    • v.6 no.1
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    • pp.34-44
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    • 2003
  • Purpose : The purpose of this study was to elucidate the effects of hospice care on pain management of the terminal cancer patients. Method : The subjects of the study were 37 terminal cancer patients hospitalized in the general hospital in JeonJu with the hospice care nit. The data were collected using the questionaire with interviews from July to Nov. in 2000. The severity and interference of pain were examined with the self reported survey based on the Korean version of Brief Pain Inventory (BPI-K). The data were analyzed with the mean, SDs, paired t-test. The hospice care to provide for three weeks. Results : 1) The mean scores of the worst pain for the last 24-hours measured with the pain severity of BPI-K were pre-intervention (6.35) and post (4.76). The pain interference of BPI-K in pre-intervention was enjoy (8.22), work (7.46), walk (7.08) and activity (7.08), while post was of enjoy (6.62), work (6.43), walk (6.11) and activity (5.78), respectively. 2) In pain severity, significant difference was found between the pre-intervention and post in all of followings. - worst pain for last 24 hours (t=4.085, P=.000) - least pain for last 24 hours (t=4.020, P=.000) - average pain for last 24 hours (t=4.254, P=.000) - pain right now (t=4.017, P=.000) 3) In pain interference, significant difference was found between the pre- intervention and the post in all of followings. - activity (t=3.137, P=.003) - mood (t=6.713, P=.000) - walk (t=2.027, P=.050) - work (t=2.132, P=.040) - relate (t=4.143, P=.000) - sleep (t=4.071, P=.000) - enjoy (t=3.881, P=.000) Conclusion : The terminal cancer patients who were offered hospice care had significantly lower hospice care pain and pain interference than those without hospice care. According to these results, hospice care can be regarded as an effective modality in relief of pain in the terminal ill-patients.

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Maximizing the Efficiency Lifetime Product for Phosphorescent OLEDs

  • Adamovich, Vadim;Kwong, Raymond C.;Weaver, Michael S.;Hack, Mike;Brown, Julie J.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.272-276
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    • 2004
  • Great strides in organic light emitting device (OLED) technology have resulted in a number of commercial products. To continue this growth into large area displays, for example televisions, an understanding of the mechanisms that drive the OLED device efficiency and lifetime performance is critical. In this work, we consider maximizing the efficiency lifetime product based on phosphorescent OLED ($PHOLED^{TM}$) technology. We report green PHOLEDs with luminous efficiency of 82 cd/A, 5.7 V and 10,000 hours lifetime at 1,000 cd/$m^2$,red PHOLEDs with CIE of (0.67,0.33), 11 cd/A and 35,000 hours lifetime at 500 cd/$m^2$ and recent progress in blue demonstrating efficiencies of 18 cd/A at 200 cd/$m^2$.

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Study on the Development of Steam Boiler using Carbon-fiber Heater (탄소섬유 발열체를 이용한 증기 보일러 개발에 관한 연구)

  • Kim, Bu-Ahn;Kang, Suk-Jun;Choi, Young-Min;Moon, Chang-Kown
    • Journal of Power System Engineering
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    • v.21 no.3
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    • pp.30-36
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    • 2017
  • Carbon fiber shows excellent heat generation value and thermal efficiency comparing with the metallic materials because of its far infrared-rays and radiant heat. So, high performance and economic steam boiler system for the industry can be manufactured by using the carbon fiber heater. The far infrared ray radiation rate was more than 90 % of carbon fiber. Steam boiler system with carbon fiber heater in this study is made up of heating section. In the proof test of steam boiler, the aimed temperature and dwelling time were at $500^{\circ}C$ for 8,000 hours, $600^{\circ}C$-3,000 hours, and $700^{\circ}C$-1,000 hours. The temperature rise rate of steam boiler with carbon fiber heater was about 40% faster than that of the conventional boiler.