• Korean Journal of Microbiology
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• v.42 no.3
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• pp.195-198
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• 2006

Lethal toxin is a critical virulence factor of anthrax. It is composed two protein: protective antigen (PA) and lethal factor (LF). PA binds to specific cell surface receptors and, forms a membrane channel that mediates entry of LF into the cell. LF is a zinc-dependent metalloprotease, which cleaves MKKs [MAPK (mitogen-activated protein kinase) kinases] at peptide bonds very close to their N-termini. In this study, we suggest application of cell-based assays in the early phase of drug discovery, with a particular focus on the use of yeast cells. We constructed MEK1 expression system in yeast to determine LF activity and approached cell-based assay system to screen inhibitors, in which the results covering the construction of LF-substrate in yeast expression vector, expression, and LF-mediated proteolysis of substrate were described. These results could provided the basic steps in design of cell-based assay system with the high efficiency, rapidly and easy way to screening of inhibitors.

• Journal of Korean Home Economics Education Association
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• v.18 no.2 s.40
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• pp.1-13
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• 2006

The main purpose of this thesis is to research various theories in Cultural Education. It is to rebuild the value of home economics, and to demonstrate its potential to bring out a true human being. Until now, home economics education had been focused on developing ability to solve problems, only to fail us in training students to have ability to cope with different conditions in daily life by themselves. With the society rapidly changing, such a temporizing education system would lose its value immediately, and the identity of home economics education is at stake today. The present situation is resulted from the failure in approaching the culture education properly. With the fact that the elements of home economics are culture themselves. the failures in organizing and teaching the subject would urge us to rearrange curriculum of home economics. We need to bear in mind that the curriculum of home economics cannot contain every cultural value. A careful consideration is necessary in order to include the essence of the culture at the minimum quantity. Through analyzing different types of theories and methods of education in Cultural Education, this research is aimed at reviewing home economics education with its concept. Further study in the future should be proceeded with more comprehensive researches on instruction and learning methods with Cultural Education perspective, based on the analysis.

• Journal of the Korean Geotechnical Society
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• v.32 no.9
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• pp.51-62
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• 2016

Parametric studies for various site conditions by using 3d numerical model were carried out in order to estimate dynamic behavior of soil-pile-structure system in dry soil deposits. Proposed model was analyzed in time domain using FLAC3D which is commercial finite difference code to properly simulate nonlinear response of soil under strong earthquake. Mohr-Coulomb criterion was adopted as soil constitutive model. Soil nonlinearity was considered by adopting the hysteretic damping model, and an interface model which can simulate separation and slip between soil and pile was adopted. Simplified continuum modeling was used as boundary condition to reduce analysis time. Also, initial shear modulus and yield depth were appropriately determined for accurate simulation of system's nonlinear behavior. Parametric study was performed by varying weight of superstructure, pile length, pile head fixity, soil relative density with proposed numerical model. From the results of parametric study, it is identified that inertial force induced by superstructure is dominant on dynamic behavior of soil-pile-structure system and effect of kinematic force induced by soil movement was relatively small. Difference in dynamic behavior according to the pile length and pile head fixity was also numerically investigated.

• KSBB Journal
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• v.20 no.1 s.90
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• pp.26-33
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• 2005

The effects of carbon sources for exopolysaccharide production during batch cultivation of an Enterobacter sp. isolated from the composter were investigated. The highest amount of exopolysaccharide was obtained when lactose was used as carbon source. Lactose in medium was converted into glucose and galactose. Glucose was metabolized fast and was completely consumed, but about $20\%$ of lactose was accumulated as galactose. On the other hand, enzyme activity was about $350\~450$ unit with the increase of lactose concentration. Thus, it was considered that the exopolysaccharide might be produced in the course of that lactose was hydrolyzed into glucose and galactose by $\beta-galactosidase$ with respect to that enzyme activity on lactose hydrolysis was accorded to the exopolysaccharide production. When glucose and galactose were added to lactose medium, respectively, it could be considered that glucose was as a repressor and galactose was as a inducer for $\beta-galactosidase$ synthesis even though the mechanisms were not elucidated. The increase of lactose concentration was almost ineffective to the specific growth rate $(0.133\~0.151\;hr^[-1})$ but showed the difference in the biomass content. The higher carbon source concentration, the more residual sugar remained. It was assumed that the optimum lactose concentration for exopolysaccharide production was $30\~70g/L.$ On the other hand, it was considered that the nitrogen acted as growth limiting nutrients to the cell growth. In the cases of 30 and 70 g/L of the fixed carbon concentrations, the increase of the nitrogen sources concentration caused a remarkable increase within the range of $0.059\~0.225\;hr^{-1}$ and $0.141\~0.237hr^{-1}$ of the specific growth rate, respectively, while there was no significant difference in biomass.

• Journal of Environmental Science International
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• v.3 no.2
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• pp.165-175
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• 1994

An emergy analysis of the main energy flows driving the economy of humans and life support systems was made including environmental energies, fuels, and imports, all expressed as solar emjoules. The total emergy use (4, 373 E20 sej/yr) is 90 per cent from imported sources, fuels and goods and services. The emergy flows from the environment are modest, because the share of global inputs such as ruin and geological uplift flux is modest. Consequently, the ratio of outside investment to attracting natural resources is already large, like other industrialized countries. The population level is already in excess of carrying capacity. The emergy use per person in Korea indicates a moderate emergy standard of living, even though the indigenous resource is very poor. If the present economy were running entirely on stored reserves of fuels, soils, woods, etc., it would last about 2 years. Its carrying capacity for steady state on its renewable sources is only 3.3 million people, compared to 43.3 million in 1991. Continued availability of foreign oil at a favorable balance of emergy trade, currently about 7 to 1 net emergy, is the basis for present economic activity and must decrease as the net emergy of foreign oil purchased goes down. Close economic integration with Middle East may determine how long this is possible in the future.

• The Korean Journal of Financial Management
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• v.2 no.1
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• pp.55-75
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• 1986

This article is to analyse the economic efficiency of capital market, which plays a role of resource allocation in terms of financial claims such as stock and bond. It provides various contributions to the welfare theoretical aspects of modern capital market theory. The key feature that distinguishes the theory described here from traditional welfare theory is the presence of uncertainty. Securities has time dimensions and the state and outcome of the future are really uncertain. This problem resulting from this uncertainty can be solved by complete market, but it has a weak power to explain real stock market. Capital Market is faced with the uncertainity because it is a kind of incomplete market. Individuals and firms in capital market made their consumption-investment decision by their own criteria, i. e. the maximization of expected utility form intertemporal consumption and the maximization of the market value of firm. We noted that allocative decisions that had to be made in the economy could be naturally subdivided into two groups. One set of decisions concerned the allocation of first-period resources among consumption $C_i$, investment in risky firms $I_j$, and riskless investment M. The other decisions concern the distribution among individuals of income available in the second period $Y_i(\theta)$. Corresponing to this grouping, the theoretical analysis of efficiency has also been dichotomized. The optimality of the distribution of output in the second period is distributive efficiency" and the optimality of the allocation of first-period resources is 'the efficiency of investment'. We have found in the distributive efficiency that the conditions for attainability is the same as the conditions for market optimality. The necessary and sufficient conditions for attainability or market optimality is that (1) all utility functions are such that -$\frac{{U_i}^'(Y_i)}{{U_i}^"(Y_i)}={\mu}_i+{\lambda}Y_i$-linear risk tolerance function where the coefficients ${\mu}_i$ and $\lambda$ are independent of $Y_i$, and (2) there are homogeneous expectations, i. e. ${\Large f}_i(\theta)={\Large f}(\theta)$ for every i. On the other hand, the efficiency of investment has disagreement about optimal investment level. The investment level for market rule will not generally lead to Pareto-optimal allocation of investment. This suboptimality is caused by (1)the difference of Diamond's decomposable production function and mean-variance valuation model and (2) the selection of exelusive investment or competitive investment. In conclusion, this article has made an analysis of conditions and processes of Pareto-optimal allocation of resources in capital marker and tried to connect with significant issues in modern finance.

• Journal of the Korean Institute of Intelligent Systems
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• v.5 no.1
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• pp.52-64
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• 1995

Robot manipulator is a highly nonlinear-time varying system. Therefore, a lot of control theory has been applied to the system. Robot manipulator has two types of control; one is path planning, another is path tracking. In this paper, we select the path tracking, and for this purpose, propose the intelligent control¬ler which is combined with fuzzy logic and neural network. The fuzzy logic provides an inference morphorlogy that enables approximate human reasoning to apply to knowledge-based systems, and also provides a mathematical strength to capture the uncertainties associated with human cognitive processes like thinking and reasoning. Based on this fuzzy logic, the fuzzy logic controller(FLC) provides a means of converhng a linguistic control strategy based on expert knowledge into automahc control strategy. But the construction of rule-base for a nonlinear hme-varying system such as robot, becomes much more com¬plicated because of model uncertainty and parameter variations. To cope with these problems, a auto-tuning method of the fuzzy rule-base is required. In this paper, the GA-based Fuzzy-Neural control system combining Fuzzy-Neural control theory with the genetic algorithm(GA), that is known to be very effective in the optimization problem, will be proposed. The effectiveness of the proposed control system will be demonstrated by computer simulations using a two degree of freedom robot manipulator.

• Journal of the Korean Institute of Intelligent Systems
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• v.17 no.4
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• pp.563-568
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• 2007

This paper presents a methodology for predicting nonlinear time series based on the neural network with weighted fuzzy membership functions (NEWFM). The degree of classification intensity is obtained by bounded sum of weighted fuzzy membership functions extracted by NEWFM, then weighted average defuzzification is used for predicting nonlinear time series. The experimental results demonstrate that NEWFM has the classification capability of 92.22% against the target class of GDP. The time series created by NEWFM model has a relatively close approximation to the GDP which is a typical business cycle indicator, and has been proved to be a useful indicator which has the turning point forecasting capability of average 12 months in the peak point and average 6 months in the trough point during 5th to 8th cyclical period. In addition, NEWFM measures the efficiency of the economic indexes by the feature selection and enables the users to forecast with reduced numbers of 7 among 10 leading indexes while improving the classification rate from 90% to 92.22%.

• KSBB Journal
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• v.23 no.6
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• pp.499-503
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• 2008

The bioethanol for use as a liquid fuel by fermentation of renewable biomass as an alternative to petroleum is important from the viewpoint of global environmental protection. Recently, many scientists have attempted to increase the productivity of bioethanol process by developing specific microorganism as well as optimizing the process conditions. In the present study, which is based on our previous investigation on the pretreatment process, theproductivity of bioethanol obtained from simultaneous saccharification and fermentation (SSF) process was compared between various domestic materials including barley, brown rice, corn and sweet potato. Additionally, Solid glucoamylase (SGA; developed in Changhae Co.), from modified strain with UV, was used. The result was compared to commercial glucoamylase (GA). It was observed that the fermentation rate was increased together with the yield which can be derived from the final ethanol concentration. Especially, in the case of brown rice, compared to the experimental results using GA, the final ethanol concentration was 1.25 times higher and 18.4 g/L of the yield was increased. Also, the time required for reaching 95% of the maximum ethanol concentration is significantly reduced, which is approximately 36 hours, compared to 88 hours using GA. It means that SGA has excellent saccharogenic power.

• Korean Journal of Construction Engineering and Management
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• v.16 no.6
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• pp.101-111
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• 2015

The modular construction method has been getting more attention followed by global eco-friendly trend as the domestic construction industry has focused more on remodeling and extension work. The modular construction method is an industrialized construction system which is not likely as the existing construction method it manufactures more than 70% modules at the factory then assembling can be completed in a short amount of time on site. The modular construction method has various strengths; shortening of construction period by on-site work decrease, weight pressure reduction by usage of light steel frames and cost saving by repetitive manufacturing. However, it is currently not expanded due to the existing commercialized construction method. Therefore, this research is in order to help the related authorities make decisions to select the construction method and motivate expansion of modular construction method which can be utilized effectively in the extension works. The intention of this research is to stress differentiation from other construction methods in construction period, construction expenses, labor and forces by comparing and analyzing actual cases, to inform competitiveness of modular construction method by concrete effect analysis and to support adoption of the modular construction method into the domestic industry.