• YAKHAK HOEJI
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• v.52 no.4
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• pp.279-282
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• 2008

Plasmids were isolated from 15 tetracycline (Tc) resistant S. aureus. Two small tetracycline resistance plasmids, pKH16 and pKH17, have been isolated from Staphylococcus aureus JY10 and Staphylococcus aureus JY22, respectively and the complete nucleotide sequences of those plasmids have been determined. pKH16 consisted of 4,442 bp and showed high identity to pKH6 (99% matching percentage) isolated in 1989 from S. aureus SA2. pKH17 consisted of 4,441 bp and showed less identity to pKH6 (95% matching percentage) than pKH16. PCR analysis showed that tetK and tetM did not exist in ten large plasmids isolated from ten Tc resistant S. aureus. Twelve Tc resistant S. aureus showed reistance both to Tc and Mn and we might analogize that twelve Tc resistant S. aureus had tetM in their chromosome.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.33-36
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• 2008

Staphylococcus aureus KH13 and Staphylococcus aureus KH28 were resistant to chloramphenicol, ampicillin, clindamycin, erythromycin, gentamicin, kanamycin, streptomycin, tobramycin, and norfloxacin. A plasmid (pKH13) and two plasmids (pKH14, pKH15) were isolated from Staphylococcus aureus KH13 and Staphylococcus aureus KH28, respectively and complete nucleotide sequences of three plasmids were determined. It was found that pKH13 and pKH15 mediated chloramphenicol resistance and pKH14 was a cryptic plasmid.

• Biomedical Science Letters
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• v.5 no.1
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• pp.131-133
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• 1999

In order to the investigate epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA), 31 strains of Staphylococcus aureus were isolated from the equipments of two hospitals in Chonbuk. And their antimicrobial resistance patterns against 7 kinds of antimicrobial agents and the identification of MRSA by polymerase chain reaction (PCR) were studied. Seven strains among 10 strains of methicillin resistant Staphylococcus aureus showed 554 bp DNA which was a part of mecA gene in PCR analysis.

• YAKHAK HOEJI
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• v.36 no.3
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• pp.255-258
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• 1992

The clinical isolate Staphylococcus aureus SA8 was resistant to tetracycline(Tc) and harboured a plasmid pKH1(24.82 kb). pKH1 was shown by curing and by transformation to specify resistance to Tc. The cleavage map of a pKH1 was determined by restricction enzyme mapping techniques. Cleavage map is given for BglII, EcoRI, HpaII, PvuII and SalI. Restriction endonuclease BamHI, BglI, BstEII, HpaI, PstI, and XhoI have no sites on this plasmid. HaeIII, XbaI, and HindIII have 5, 6, 14 sites, respectively.

• The Journal of the Korean Society for Microbiology
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• v.21 no.1
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• pp.145-149
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• 1986

The development of delayed-type hypersensitivity(DTH) reaction to Staphylococcus aureus in mice was studied, Mice received 3 injections of $10^8$ viable S. aureus subcutaneously showed a marked footpad swelling when mice were challenged with $10\;{\mu}g$ staphylococcal protein antigen into footpad(The percent increase of footpad thickness at 24 h after challenge wsa 35% approximately). Histological observation of footpad of immunized mice showed a marked thickness of subcutaneous tissue due to edematous reaction and massive infiltration of lymphocytes and neutrophils which are characteristic cells in DTH reaction. Intensity of DTH reaction of mice immunized with viable bacteria was much higher than that of mice immunized with staphylococcal protein or heat-killed bacteria. The DTH reaction to S. aureus could be transferred to normal recipient mice by both spleen cells and lymph node cells.

• YAKHAK HOEJI
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• v.37 no.6
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• pp.621-624
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• 1993

The clirical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEll, Hindlll, Hpall, and Xbal. The above restriction endonucleases have a single site, but nucleases BamHl, Bgll, BglII, EcoRl, EcoRV, HaeIII, Hpal, Kpnl, Pstl, PvnII, Sall, Smal, and XhoI have no site on this plasmid.

• YAKHAK HOEJI
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• v.36 no.5
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• pp.486-490
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• 1992

The clinical isolate Staphylococcus aureus SA2 was resistant to ampicillin, Chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, and tobramycin and harboured more than two kinds of plasmids. Transformation experiment demonstrated that 40.98-kb plasmid(pKH2) encoded resistance to ampicillin, clindamycin, erythromycin, kanamycin, and streptomycin. The cleavage map of a pKH2 was determined by restriction enzyme mapping techniques. Cleavage map is given for BamHI, BglI, BstEII, SalI and XhoI.

• YAKHAK HOEJI
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• v.51 no.3
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• pp.164-168
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• 2007

Antibiotic resistance patterns of 21 antibiotics were studied for 50 strains of Staphylococcus aureus isolated from a hospital in Busan from July 2005 to December 2006. All strains showed antibiotic resistance to more than one antibiotic and 3 strains showed resistance to 17 different antibiotics. The strains isolated between 2005 and 2006 had lower resistance rate to 12 antibiotics (other than vancomycin and ampicilin) than the strains isolated between 1989 and 1990. In particular, no chlorarmphenicol resistant strain was found in this study which is contrasted with 34.8% resistant rate obtained in the study conducted between 1989 and 1990. In respect of vancomycin, no resistant strain was found in this study which is the same result obtained in the 1989 to 1990 study; All strains investigated in this study showed 100% resistance rate to ampicillin compared to 69.6% in the previous study.

• YAKHAK HOEJI
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• v.52 no.2
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• pp.147-150
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• 2008

Previously, we reported on the antibiotic resistance patterns of 50 strains of Staphylococcus aureus which were isolated from a hospital in Busan, Korea from July 2005 to December 2006. We have isolated small plasmids and classified plasmid types by agarose gel electrophoresis. We have selected 5 plasmids and determined complete nucleotide sequences of those plasmids. The aim of this paper is to report on the characteristics of cadmium, erythromycin, lincomycin resistance plasmids and a cryptic plasmid based on the sequence analysis obtained by using the BLAST program.

• Journal of the Korean Chemical Society
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• v.53 no.5
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• pp.613-619
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• 2009