• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.95-101
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• 2004

This study was undertaken to study the effect of interspecific pollination of L. longiflorum and L. ${\times}$formolongi as the female parent with Oriental, Martagon and Trumpet group as the male parent by cut-style pollination. In the interspecific pollination of L. longiflorum cv. Gelria and Lorina with Oriental group as the male parent, the corresponding fruits obtained immature embryo were 1, 8, and the mean number of embryo per fruit were 11.0, 3.0, respectively. In the interspecific pollination of L. ${\times}$formolongi cv. Raizan, the corresponding fruits obtained immature embryo were 17, and the mean number of embryo per fruit were 4.0. On the other hand, in the interspecific pollination between L. longiflorum and L. ${\times}$formolongi as the female parent and Martagon and Trumpet group as the male parent, the pollination of L. ${\times}$formolongi as the female parent and L. henryi of Trumpet group as the male parent were obtained only 2 fruits, however no embryo.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.45-52
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• 2016

In order to select excellent cross parents for development new cultivar of low temperature tolerant, combining ability was conducted by 24 cross combinations obtained from crosses between 'Dahong', 'Gamhong', 'Maehyang', 'Seolhyang' as seed parents and 'Dahong', 'Gamhong', 'Maehyang', 'Sugyeong', 'Sunhong', 'Wongyo 3111' as pollen parents. The results showed that two cultivars of 'Dahong', 'Gamhong' were not suitable for seed parents. Because average fruit weight in case of 'Dahong' as a seed parent was the tendency to become the smallest in total cross combinations, survival ratio in case of 'Gamhong' as a seed parent was the lowest in total cross combinations. And fruit hardness in case of 'Sunhong' as a pollen parent was the tendency to low, incidence of malformed fruit in case of 'Sugyeong' as a pollen parent was the tendency to be increased. Therefore, two cultivars of 'Sunhong', 'Sugyeong' was also not suitable for pollen parents. But In case of 'Maehyang' and 'Seolhyang' as seed parents, germination percent and survival rate were relatively higher than other seed parents, fruit quality and yield had also excellent. Therefore, there could be selected to 'Maehyang' and 'Seolhyang' as seed parents and 'Maehyang', 'Seolhyang', 'Wongyo3111' as pollen parents. Especially, the crosses 'Maehyang' × 'Seolhyang', 'Seolhyang' × 'Maehyang', 'Seolhyang' × 'Wongyo3111' were suitable for cross combination for development new cultivar of low temperature tolerant to be demonstrated the excellence as cross parents in sugar contents, fruit weight, fruit hardness and yield.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.1
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• pp.62-66
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• 1984

One row of pollen parent to two rows of seed parent (1:2), 2:4 and solid (1:2) planting patterns (PP) were compared in seed parent densities of 3,500, 5,000 and 6,500 plants per 10 ares to determine effects of PP and plant density on growths of seed and pollen plants, and seed yield of seed parent of modified single cross corn hybrid. Planting pattern did not significantly affect agronomic characteristics of seed plant except ear number per 100 plants and seed yield which were greater in solid and 1:2 PP than in 2:4 PP. Significant PP x plant density interaction did not exist for agronomic characteristics of seed parent. In the seed parent, plant height and 100 kernel weight were not affected by plant density, but ear height, ear number per 100 plants, and kernel number per ear were linearly decreased with increased plant densities. Seed yield ranged from 330 to 460 kg per 10 ares and overall yield response to plant density was quadratic. Tassel length and spikelet number per tassel of the pollen parent were significantly affected by PP and plant density. Significant PP x plant density interaction existed for tassel length and spikelet number per tassel. Tassel length and spikelet number per tassel were greater in 1:2 and 2:4 PP compared to solid PP and were greatly reduced with increased plant densities in solid and 1:2 PP. The results indicated that 1:2 or 2:4 PP at around 5,000 plants per 10 ares for seed parent would be suitable for seed production of modified single cross com hybrid.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.226-231
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• 2010

The objective of this experiment was determined to investigate the effect of the planting ratio of Male Sterility (MS) to Restorer Line (RL) and harvesting time on fatty acid compositions under $F_1$ seed production of Brassica napus L. For rapeseed seed production, two experiments were conducted in the open fields. One experiment studied planting ratios of MS to RL (4:2, 10:2, or 10:1) were planted and investigated fatty acid composition at 40, 45, 50, 55, and 60 days after flowering, the other $F_2$ seeds were analyzed on fatty acid compositions of harvested seeds at five sequential stages. The results showed that fatty acid compositions of developing seeds were influenced by MS:RL planting ratios and $F_2$ hybrid treatments and contaminated level of fatty acid compositions, erucic acid, were unaffected by planting ratio of MS to RL. Fatty acid compositions such as palmitic acid (C16:0), stearic acid (C18:0) and linoleic acid (C18:2) contents decreased during seed maturation period in $1^{st}$ and $2^{nd}$ experiments. In contrast, oleic acid (C18:1) content relatively increased up to 55days after flowering. At day 60 after flowering, oleic acid content was unaffected by MS:RL planting ratios and $F_2$ seeds treatments. Aspects of related gene expression of fatty acid synthesis such as SAD, FAD1 and FAD2 were followed exactly to changes of fatty acid composition during seed maturation. These results suggest that MS ratio may be enlarged and RL may be reduced, indicating this ratio will be useful for rape seed production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.4
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• pp.73-80
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• 1980

To facilitate the heterosis breeding which utilizes cytoplasmic-genetic male sterile lines in rape (Brassica napus L.), 511 improved domestic lines were tested for their fertility restoration, maintaining ability and heterosis expression in agronomic characters. About 81 % of 511 lines showed complete fertility restoration and 11 lines could be used as maintainer of male sterility. All yield components except pod length showed greater heterotic effects than that of midparent in $F_1$. These heterotic effects resulted in extraordinary yielding ability as high as 5.6 T/ha in several $F_1$`s.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.138-145
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• 1988

The effect of directions (north to south vs east to west) of planting row, planting intensities (10/15, 15/15, 20/15, 25/15 cm/cm) and the number of rows consecutively (1,2, 3,4) for both the CMS and pollen parent in alternation, on the seed yield of CMS of rice was tested with a breeding line V20A/Iri342$\^$*8/. When the planting row was made in vertical direction to the wind direction during anthesis, the seed yield was higher than the other direction regardless the planting densities and number of rows consecutive. Seed yield increased as high as 46.9%. The higher planting intensities up to 10/15 cm/cm yielded the higher hybrid seeds. The highest yield was 81.2 kg/ha. The yield variation among plants within a plot and among the plants within a replication was relatively large. Increased pollen parent row numbers caused increased grain fertility per plant, but the seed yield was increased by reduced pollen parent row number. Reduced CMS parental row numbers caused increased grain fertility per plant, but the seed yield was increased by increased CMS parental row numbers. Considering the hybrid seed yield, parental seed yield and operational convenience, 4 rows of CMS and 1 row of pollen parent in alternate with 20/15 or 25/15 (cm/cm) planting densities seemed to be the rational layout.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.178-182
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• 2005

To identify the seedlings from controlled pollination between one paternal tree and three maternal trees of Japanese red pine, cpSSR markers of the paternally inherited haploid genome were analyzed in two year old 114 seedlings of full sib families. Individual specific DNA fingerprint like haplotypes of the parental trees were determined by PCR with three cpSSR primers. Haplotypes of the 114 seedlings were also identified by PCR with the same primers. On the basis of the comparison of cpDNA haplotypes of the 114 seedlings with those of the parental trees, 14 seedlings revealed to have distinguished haplotypes from those of the paternal tree. It was tentatively concluded that they were generated via pollination with the non-paternal trees. A seedling of Gangwon30 revealing non-paternal haplotype might have been generated via self pollination with the pollens of maternal tree through improper emasculation or contamination during artificial pollination. DNA fingerprint like cpSSR profiles observed in this study could be successfully applied to the various plant forensic analyses, such as identification of siblings of individual trees, asexually reproduced ramets of a specific clone, vegetatively propagated individuals via tissue culture, and pure full sib progenies.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.598-604
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• 2015

This study was carried out to develop molecular techniques to allow the selection of zygotic seedlings in the early stage of the plant development. We identified 37 pollen-specific molecular markers from RAPD analysis and successfully used them for identification of the zygotic seedlings from various hybrid crosses. Three Satsuma mandarin cultivars ('Morita unshiu', 'Nangan 20' and 'Miyagawawase') were used as mother parents and seven cultivars ('Ponkan', 'Lee', 'Kinokuni', 'Shiranuhi', 'Tamnaneunbong', 'Shinyegam', and 'Sunburst' mandarins) served as pollen parents. PCR analysis showed that 2 primers could identify zygotic hybrid seedlings. Among them, an UBC-27 primer was used to identify the zygotic seedlings from hybrid crosses of "'Nangan 20' ${\times}$ 'Kinokuni'" mandarin, "'Nangan 20 ${\times}$ Ponkan'" mandarin and "'Miyagawawase ${\times}$ Sunburst'" tangerine. In total 29 out of 40 seedlings (73%), 9 out of 47 seedlings (19%), and 13 out of 45 (29%) were identified as zygotic seedlings, respectively. These results can show that the pollen-specific markers selected in this study can be used effectively for early identification of zygotic seedlings from Citrus hybrid crosses.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.51-51
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• 2018

본 연구는 바이오에너지용 이질3배체 억새(Miscanthus ${\times}$ giganteus) 품종육성 위한 교배재료인 물억새(M. sacchariflrous)와 참억새(M. sinensis) 출수기 단축과 단교배 방법을 개발하기 위해 수행하였다. 물억새와 참억새를 12시간 일장, 자연일장 조건에서 재배하여 출수기 단축에 미치는 단일효과를 조사하였다. 출수기에 일중 화분 발아시간, 절단한 화분친 이삭 활력 유지 및 격리방법 등 단교배 방법을 구명하여 이를 활용한 억새의 자가 수정 여부를 조사하고 시험교배를 실시하였다. 참억새와 물억새 모두 12시간 일장의 단일 조건에서 재배한 것이 자연일장에 재배한 것에 비해 출수 소요일수가 18~27일 정도 단축되었다. 화분 발아는 물억새에서 오전 6시에 왕성하였으나 시간이 경과할수록 발아율이 낮아져 오전 8시에는 10% 이하만 발아하였다. 참억새 화분은 오전 6시에는 발아하지 않았으나 오전 7시에 50% 이상이 발아하였고 8시에는 물억새와 같이 10% 이하였다. 화분친 참억새 이삭을 절단, 절화 보존액에 꽂아 백색 부직포로 격리하였을 때 절화 보존액량이 많을수록 활력 유지 일수가 증가하여 150 mL에서 물억새, 참억새 모두 7일간 개화 및 화분 비산을 지속하였다. 이 때 화분 발아율은 참억새와 물억새 모두 4일까지 40%를 유지하였다. 참억새와 물억새는 자가수정율이 ~ 2.5%로 낮고, 자연교잡 임실율은 출수기가 빠른 유전자원에서 54.4%까지 높았다. 상기 단교잡 방법을 적용한 물억새 4배체와 참억새 2배체간 14조합 시험교배로 437립의 종자를 얻었다. 본 연구의 억새 종간 단교배 방법은 우수한 종자친과 화분친으로 교배종자를 얻을 수 있어 향후 바이오매스 수량이 많으면서, 종자가 맺히지 않은 이질 3배체 품종육성에 활용할 수 있을 것으로 판단된다.

• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.264-271
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• 2013

Mating system parameters were estimated in a natural population of Pinus koraiensis which was located at Gwongeumseong in Mt. Seorak, South Korea. The estimated parameters from allozyme were as follows: 0.882 of multilocus outcrossing rates($t_m$), 0.881 of singlelocus outcrossing rates($t_s$), 0.368 of correlated paternity($r_p$), and 2.7 of number of effective pollen contributors. The estimated parameters from cpSSR markers were as follows: 0.831 of average of outcrossing rates and 12.4 of the average number of effective pollen contributors. The average outcrossing rate from two genetic markers was 0.857, which was similar to those estimated in other conifer species. More number of potential pollen contributors was estimated from cpSSR marker analysis compared with that estimated from allozyme marker analysis. This result sugges$t_s$ that cpSSR markers may be more useful than allozyme markers for identifying potential pollen contributors in the analysis of mating system.