Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Early Identification of Citrus Zygotic Seedlings Using Pollen-specific Molecular Markers

화분 특이적 마커를 이용한 감귤 교잡종 실생묘의 조기 동정

  • Jin, Seong Beom (Citrus Research Institute, National Institute of Horticultural & Herbal Science RDA) ;
  • Yun, Su Hyun (Citrus Research Institute, National Institute of Horticultural & Herbal Science RDA) ;
  • Park, Jae Ho (Citrus Research Institute, National Institute of Horticultural & Herbal Science RDA) ;
  • Park, Suk Man (Citrus Research Institute, National Institute of Horticultural & Herbal Science RDA) ;
  • Koh, Sang Wook (Fruit Research Division, National Institute of Horticultural & Herbal Science RDA) ;
  • Lee, Dong Hoon (Planning and Coordination Division, National Institute of Horticultural & Herbal Science RDA)
  • 진성범 (농촌진흥청 국립원예특작과학원 감귤연구소) ;
  • 윤수현 (농촌진흥청 국립원예특작과학원 감귤연구소) ;
  • 박재호 (농촌진흥청 국립원예특작과학원 감귤연구소) ;
  • 박석만 (농촌진흥청 국립원예특작과학원 감귤연구소) ;
  • 고상욱 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 이동훈 (농촌진흥청 국립원예특작과학원 기획조정과)
  • Received : 2014.12.21
  • Accepted : 2015.04.01
  • Published : 2015.08.31
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Abstract

This study was carried out to develop molecular techniques to allow the selection of zygotic seedlings in the early stage of the plant development. We identified 37 pollen-specific molecular markers from RAPD analysis and successfully used them for identification of the zygotic seedlings from various hybrid crosses. Three Satsuma mandarin cultivars ('Morita unshiu', 'Nangan 20' and 'Miyagawawase') were used as mother parents and seven cultivars ('Ponkan', 'Lee', 'Kinokuni', 'Shiranuhi', 'Tamnaneunbong', 'Shinyegam', and 'Sunburst' mandarins) served as pollen parents. PCR analysis showed that 2 primers could identify zygotic hybrid seedlings. Among them, an UBC-27 primer was used to identify the zygotic seedlings from hybrid crosses of "'Nangan 20' ${\times}$ 'Kinokuni'" mandarin, "'Nangan 20 ${\times}$ Ponkan'" mandarin and "'Miyagawawase ${\times}$ Sunburst'" tangerine. In total 29 out of 40 seedlings (73%), 9 out of 47 seedlings (19%), and 13 out of 45 (29%) were identified as zygotic seedlings, respectively. These results can show that the pollen-specific markers selected in this study can be used effectively for early identification of zygotic seedlings from Citrus hybrid crosses.

본 연구는 종자친과 화분친을 구별할 수 있는 분자마커를 개발하고 이를 이용하여 교잡종 실생묘를 조기 선발하는 프로토콜을 확립하고자 본 연구를 진행하였다. 종자친으로 3종의 온주밀감('성전온주', '남감20호', '궁천조생')과 화분친으로 7종의 만다린 감귤('병감', 'Lee', '기주밀감', '신예감', '부지화', '탐나는봉', 'Sunburst') 품종을 사용하여 RAPD 분석방법을 이용하여 교잡종 실생묘를 선발 할 수 있는 화분친 특이적 프라이머 37개를 선발하였다. PCR 분석결과 이중 2개의 프라이머는 모든 교배조합에서 교잡종 실생묘를 선발할 수 있었다. 또한 "남감20호 ${\times}$ 기주밀감", "남감20호 ${\times}$ 병감" 그리고 "궁천조생 ${\times}$ Sunburst"의 교잡종 실생묘는 UBC 27프라이머를 사용하여 각각 40개체 중 29개체(73%), 47개체 중 9개체(19%) 그리고 45개체 중 13개체(29%)의 교잡종 실생묘를 선발할 수 있었다. 위 결과는 화분친 특이적인 마커를 사용하여 감귤에서 효과적으로 교잡종 실생묘를 동정 할 수 있음을 보여준다.

Keywords

References

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