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Evaluation of Genetic Safety in Genome-editing Rice Through Comparative Analysis of Genetic and Agronomic Traits (유전적 특성과 농업형질의 비교분석을 통한 유전자 교정 벼의 안전성 평가)

  • Seung-Kyo Jeong;Dohyeong Gwon;Bae-Hyeon Lee;Jeong-Hwan Suh;Rahmatullah Jan;Jae-Ryoung Park;Taehun Ryu;Kyung-Min Kim
    • Journal of Life Science
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    • v.34 no.8
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    • pp.567-575
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    • 2024
  • New breeding techniques (NBT) recognize specific DNA sequences and remove, modify, or insert DNA at a desired location, and can be used to treat genetic diseases in humans or to improve the traits of livestock or crops. In this study, we conducted a comparative analysis of various agricultural traits and assessed the safety of gene transferability in third-generation genome-editing rice (OsCKq1-G3) with T and G nucleotide insertions developed using the CRISPR/Cas9 SDN-1 method, in comparison to its parental line (Oryza sativa L., cv Ilmi). The analyzed traits included heading date, culm length, panicle length, tiller number, yield, germination rate, viviparous germination rate, shattering, after wintering seed viability, the presence of toxins and allergens. The target trait, heading date, exhibited a high significant difference of approximately 5 days. Culm length, panicle length, tiller number, yield showed no significant differences compared to the parental line. No T-DNA bands indicating gene transfer were detected. In the third generation of genome-edited rice, the T-DNA was confirmed to be eliminated as successive generations advanced through self-pollination. Through the analysis of germination rate, viviparous germination rate, shattering, and after wintering viability, we confirmed that the genome-editing rice has no potential for weediness. The ORF and amino acid sequences of the genome-editing rice did not reveal any toxins and allergens. The results of this study can be utilized as important data for the environmental risk assessment of genome-editing rice.

Characteristics Evaluation of Combustion by Analysis of Fuel Gas Using Refuse-derived Fuel by Mixing Different Ratios with Organic and Combustible Wastes (배연가스 분석에 의한 가연성과 유기성폐기물을 혼합한 고형화연료 연소 특성평가)

  • Ha, Sang-An
    • Journal of the Korea Organic Resources Recycling Association
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    • v.17 no.3
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    • pp.27-39
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    • 2009
  • The main objective of this study is to investigate the characteristics of combustion by analyzing fuel gases from a combustion equipment with various combustion conditions for refuse-derived fuels (RDFs). CO gas is a parameter for indicating of incomplete combustion during a combustion process. The lowest CO gas was produced when the experiment conditions were m=2 under air-fuel condition and $800^{\circ}C$. $CO_2$ gas is a final product after complete combustions. The highest amount of $CO_2$ gas was produced when the experiment conditions were m=2 under air-fuel condition and $800^{\circ}C$. The highest level of $SO_2$ gas was produced in S.1 sample containing the highest sulfur. The highest level of NOx gas was produced in S.1 sample with the highest nitrogen content and air-fuel condition of m=2 under temperature of $800^{\circ}C$. HCl gas that is generated by reacting with metals catalyst through oxygen catalyst reaction during combustion process is a precursor of dioxin formation. The higher level of HCl gas was produced in the sample with higher chlorine content. The lowest level of HCl gas was produced when the experiment conditions were air-fuel condition of m=2 and $800^{\circ}C$. The lowest level of $NH_3$ gas was generated when the experiment condition was m=2 under air-fuel condition and after 3 minutes. Air-fuel condition is more important to create $NH_3$ gas than operating temperatures. Higher level of $H_2S$ gas was generated in S.1 sample with the higher sulfur content and was created in RDFs that contain higher mixture ratios of sewage sludge and food wastes. A result of combustion, gases and gases levels from the combustion of S.1 and S.2 were very similar to the combustion of a stone coal. As results of this research, when evaluating the feasibility of the RDFs, the RDFs could be used as auxiliary and main fuels.

Analysis of domestic and overseas coastal groundwater management laws and policies (국내외 해안 지하수관리 법·정책 사례 분석)

  • Shim, Young-Gyoo;Chung, Il-Moon;Chang, Sun Woo
    • Journal of Korea Water Resources Association
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    • v.57 no.9
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    • pp.633-643
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    • 2024
  • Many coastal countries have developed and used a wide range of technologies and policy measures to protect freshwater aquifers and groundwater resources from seawater intrusion, and have established and implemented a foundation to legally and institutionally support them. This study covers coastal states in the eastern United States, the Netheland, India and Japan. The goal of this study is to analyze each country's legal and policy measures for coastal groundwater management. By introducing Jeju Island's groundwater standard level system, we aim to provide a basis for future discussions on groundwater management measures not only in Jeju Island but also in coastal areas of Korea. As a result of the analysis, despite the various contents and aspects of coastal groundwater management based on local issues and characteristics around the world, in order to achieve the common goal of securing a stable amount of groundwater withdrawal and preventing seawater intrusion and to maximize the efficiency of groundwater management, it is understood that attempts are being made to establish optimal management measures, laws, systems, and policies based on several key factors. First, considering the hydrogeological characteristics and status of coastal groundwater, a separate special management system is being established and implemented within the scope of the national groundwater management system. In addition, preventing and maintaining groundwater level decline through limiting the amount of groundwater withdrawal and preventing seawater intrusion are key policy goals and policy tools, and it is suppored by research and development. Finally, tt was found that synergy effects are being sought by using various other policy tools and measures in a complex manner.

Folate intake in Korean adults: analysis of the 2016-2018 Korea National Health and Nutrition Examination Survey with newly established folate database (한국 성인의 엽산 섭취실태: 새로 구축한 식품 엽산 함량 데이터베이스를 이용한 2016-2018 국민건강영양조사 자료 분석)

  • Eun-Ji Park;Inhwa Han;Kyoung Hye Yu;Sun Yung Ly
    • Journal of Nutrition and Health
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    • v.57 no.4
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    • pp.418-434
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    • 2024
  • Purpose: The nutritional status of folate in Korean adults was evaluated using the newly established folate database (DB) and data from the 7th Korea National Health and Nutrition Examination Survey. Methods: This study analyzed the folate intake of 15,054 people (6,278 men and 8,776 women) and the relationship with serum folate concentration of 5,260 people (2,272 men and 2,988 women). Results: The average daily folate intake among Korean adults was lowest in the 19 to 29-year age group and highest in those in their 50s. Folate intake was higher in groups with higher education and household income, non-smokers, participants in aerobic physical activity, and dietary supplement users regardless of sex. Among men, office workers consumed more folate than physical workers. Vegetables and grains were the first and second most contributing food groups to folate intake. The serum folate levels were higher in women than men and lowest in the 19-29 year age group for both sexes. After adjusting for energy intake, age, income, smoking, physical activity, and dietary supplement intake, serum folate concentration increased significantly as intake increased (p < 0.001). The explanatory power (R2) of folate intake on the blood folate concentration was 0.183 and 0.141 in men and women, respectively. Conclusion: The proportion of participants consuming less than the estimated average requirement was 48.1% and 65.3% in men and women, respectively. In particular, the folate intake and serum levels of young men aged 19-29 years were the lowest. Therefore, it is necessary to improve their folate nutritional status through a balanced diet. In addition, the newly established folate DB may be useful for evaluating the folate nutritional status of Koreans.

The Optimum Photoperiod on Floral Differentiation of French Marigold Grown in a Closed-type Plant Factory (완전제어형 식물공장에서 재배되는 프렌치매리골드의 화아분화를 위한 최적의 광주기 구명)

  • Nayoung Kwak;Bo Hyun Sung;K.P.S. Kumaratenna;Young-Yeol Cho
    • Journal of Bio-Environment Control
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    • v.33 no.1
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    • pp.71-78
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    • 2024
  • Among the various environmental conditions necessary for growing crops, light is closely related to the anthesis. This study aimed to determine the optimal photoperiod affecting floral differentiation in an edible flower, marigold, to efficiently cultivate the crops in a closed-type plant factory. The experiment was conducted with photoperiods of 4, 8, 12, and 16 hours. French marigold (Tagetes patula L.) 'Durango Red' seeds were sown in polyurethane sponges, and the photoperiod treatments were applied immediately. The extent of floral differentiation was examined at 2-3 day intervals, defined as the visible appearance of flower buds at least 2 mm in size. The growth parameters such as shoot fresh weight and dry weight, height, and leaf area were measured. The optimal photoperiod was determined based on the days when the floral differentiation had occurred in 50% of the total plants. In the 4-hour treatment, proper growth and flower buds did not appear. From the 8-hour treatment, the plant grew normally, and floral differentiation occurred, however, the 8-hour treatment showed the slowest floral differentiation compared to the 12 hours treatments or more. The 12- and 16-hour treatments didn't show significant differences in floral differentiation. While the 16-hour treatment exhibited the highest results in all growth parameters, it was not significantly different from the 12-hour treatment except for shoot dry weight and leaf area. According to the results, 8 hours of photoperiod induced floral differentiation. However, more time was required for flower bud formation, and plant growth was significantly lower compared to photoperiods of 12 hours or more. Considering the energy consumption and its growth, the optimal photoperiod for marigold was 12 hours.

The Determinants of Global Technological Competitiveness of Korean SMEs: The Moderating Effect of Product Life Cycle (혁신형 중소기업의 글로벌 기술경쟁력 결정요인: 제품수명주기의 조절 효과)

  • Changsu Kim;Jong-Hun Park
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.19 no.4
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    • pp.41-53
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    • 2024
  • The Korean government's SME policy has shifted from the traditional focus of protecting SMEs against large business groups to foster innovative SMEs by upgrading their innovation base. From the government's policy perspective, innovative SMEs are defined as SMEs that can secure technological competitiveness or have the potential for future growth through technological innovation activities. Based on the resource-based theory and resource dependence theory, this study aims to explain whether both R&D capabilities of innovative SMEs and the acquisition of venture certification have a significant impact on enhancing global technological competitiveness. According to the resource-based theory, differences in a firm's tangible and intangible resources and R&D capabilities determine its innovation activities and performance. On the other hand, the resource dependence theory argues that government certification programs to support SMEs positively affect SMEs' innovation activities and performance by increasing their access to the external resources needed for innovation. Going a step further, this study examines the moderating effect of the product life cycle. As a moderating variable, the product life cycle is expected to have an opposite effect on the internal and external resource utilization. The results based on the SME Technology Statistics(2020) support the direct effect of both R&D capability and venture certification on global technological competitiveness and the differential moderating effect of product life cycle. The finding that the technological competitiveness effect of internal resource utilization is greater for longer product life cycles, while the technological competitiveness effect of external resource utilization is greater for shorter product life cycles suggests that product life cycle should be considered as an important contextual variable when studying the relationship between R&D, technological innovation, and performance. These results also suggest that in a global competitive environment where product life cycles are dramatically shortening, innovative SMEs that suffer the dual liabilities of newness and smallness should focus not only on strengthening internal R&D capabilities but also on strategically securing external resources.

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Impact of Industrial Property Rights and Innovation Capabilities on Performance: Focusing on Venture Firm Confirmation System (산업재산권 및 혁신역량이 성과에 미치는 영향: 벤처기업확인제도 혜택을 중심으로)

  • Yim Kwang-hyuk;Choi Sang-ok
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.19 no.4
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    • pp.243-254
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    • 2024
  • In recent years, as support for venture companies and performance increase, investment scale increases. To support them goverment operates venture firm confirmation system as a part of a variety of policies. Many studies suggest venture confirmation system offer the practical assistance on performance of venture companies. However, simply venture firm confirmation system affects on performance of venture companies. This study sought to develop a theoretical research model about the impact of innovation capabilities and industrial property rights on venture companies' financial performance and technological capabilities through the venture company confirmation system. The following hypotheses were tested. First, the innovation capabilities of venture companies such as development capability, manufacturing capability, marketing capability, level of cost leadership, level of high added value, degree of clarity of business model, and degree of innovation of business model have a significant impact on the venture company confirmation system. Second, the industrial property rights of venture companies, such as the number of domestic industrial property rights, foreign industrial property rights, industrial property rights in domestic applications, and industrial property rights in overseas applications, do not have a significant impact on the venture business confirmation system. Third, the benefits of the venture business confirmation system, such as promotion of technology development, advertising effects, tax benefits, securing excellent human resources, financing and investment incentives, advantageous participation in government support systems, and deregulation, are related to the financial performance of venture businesses such as sales and operating profit., net profit and technological level. Lastly, it was confirmed that, except for research and development type venture companies, innovation capabilities and industrial property rights do not have a significant impact on financial performance and technological capabilities through the venture confirmation system. The implications of this study mean that in situations where a company's innovation capabilities are lacking, the supply and demand of the venture business confirmation system is weak. Therefore, in order to improve the benefits of the venture business verification system, it is necessary to operate the venture business verification system benefits mainly for companies with high corporate capabilities. Next, it means that industrial property rights are not related to the venture business confirmation system. Therefore, there will be no need to consider industrial property rights as an important matter in the certification of the venture business verification system. Lastly, the higher the level of benefits from the venture business confirmation system, the greater the company's performance and technological capabilities. Therefore, efforts should be made to utilize the venture business verification system in a way that can improve performance or technology through the benefits of the venture business verification system.

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The Impacts of Exclusion from Natural Park Districts by Park Re-planning on Prices and Construction Activities of Private Lands (자연공원 재계획에 따른 공원구역 해제가 사유지 지가와 건축행위에 미치는 영향)

  • Sung-Woon Hong;Woo Cho;Chan-Yong Sung
    • Korean Journal of Environment and Ecology
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    • v.38 no.4
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    • pp.416-425
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    • 2024
  • This study aims to analyze the changes in land prices and building construction activities before and after exclusion from park district as results of natural park re-planning. Seoraksan National Park, Namhansanseong Provincial Park, and Cheonmasan County Park were selected as study areas, and prices and construction activities were compared between areas remaining in and areas excluded from park districts for ten years after park re-planning. Land prices increased in all three study parks. The largest difference in land prices between remaining and excluded areas occurred in Cheonmasan City Park. Land price increased more in excluded than remaining areas in Seoraksan National Park. Unlike these two parks, the changes in land prices were not much different between remaining and excluded areas in Namhansanseong Provincial Park, which can be attributed to the facts that 1) provincial parks were already developed to certain level even before the exclusion due to its less stringent land use regulation than national parks, and 2) that Namhansanseong Provincial Park was also designated as Restricted Development Zone that has similar land use regulation level to natural parks. Comparison between building density measures before and after exclusion shows that development density generally increased after the exclusion. Building heights mostly increased during 10 years after the exclusion. Building to land ratios and floor area ratios also increased. However, building to land ratios and floor area ratios increased only slightly in Namhansanseong Provincial Park and Cheonmasan City Park, suggesting that in provincial and city parks, land development already occurred as a result of less stringent land use regulation. In conclusion, a national park system significantly restricts property right in natural parks, especially in national parks, which make it difficult to expand existing natural parks and/or establish new natural parks. A remedy for resolving problems related to private lands, such as increasing budget for purchasing private lands and introducing park facilities for local community is urgently required.

The Impact of Edu-Tech and Tangible and Intangible Services of Private Institutes on parents' Intention for Re-Enrollment: The Moderating Effect of Rapport-Building Behavior (학원의 에듀테크특성과 유·무형적서비스가 학부모의 재수강의도에 미치는 영향: 라포형성행동의 조절효과)

  • Jeon, Ji-Yeon;Ha, Tae-Kwan
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.19 no.4
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    • pp.127-139
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    • 2024
  • This study investigates the impact of edutech characteristics and both tangible and intangible educational services on the intention to re-enroll, which is directly related to the management performance of private institutes. The study aims to propose strategies to improve re-enrollment intentions and management performance based on the findings. Private education has grown continuously, complementing the limitations of public education and increasing parental dependence. This study tested the hypothesis that edutech characteristics, intangible services, and tangible services, increasingly utilized with the development of information and communication technology, would influence re-enrollment intentions. It also examined whether rapport-building behavior with parents would have a moderating effect on this relationship. The hypothesis testing results showed that among the edutech characteristics, content, intangible services such as reliability and empathy, and tangible services such as tangibility and payment accessibility positively impacted re-enrollment intentions. The hypothesis that rapport-building behavior would moderate the relationship between educational services and re-enrollment intentions was supported for empathy in intangible services and tangibility in tangible services. Based on these findings, the study proposed three strategies to improve management performance of private institutes. First, in terms of improving and managing edutech characteristics, it suggested introducing and updating edutech content and ensuring operational stability. Second, for improving and managing intangible services, it recommended managing instructor recruitment and training to enhance quality and competence, maintaining professionalism through continuous education by credible institutions, and providing level-based education for students based on the qualitative improvement of educational programs. Third, to improve and manage tangible services, it suggested setting appropriate tuition fees, offering various payment methods (online, mobile, card, bank transfer) unrestricted by time and place, and equipping interiors and facilities that enable focused learning. Additionally, considering the moderating effect of rapport-building behavior, it emphasized that improvements and management requiring costs are necessary, but making parents feel a high level of tangibility through rapport-building is also important. Furthermore, given the increasing importance of edutech based on information and communication technology, the study highlighted the need for various support measures such as government technological support and venture certification system support for institutes with an entrepreneurial spirit aiming to introduce innovative technologies such as AI technology based on large language models and AR/VR-applied metaverse environments. This study is expected to help improve the management performance of private institutes by specifically suggesting items and methods for improvement and management in the educational field.

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The effect of adult learners' motivation to participate in hair education and program satisfaction on lifelong learning happiness index (성인학습자의 헤어교육 참여동기와 프로그램 만족도가평생학습 행복지수에 미치는 영향)

  • Lim Soon Ja
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.5
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    • pp.367-372
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    • 2024
  • It was intended to explore the relationship between motivation for participation in hair education, program satisfaction, and lifelong learning happiness index for adult learners at the Lifelong Education Center, and to provide basic data for revitalizing lifelong education programs. Today, universities have conditions as a comprehensive educational institution with practicality that can meet various and high-quality lifelong educational needs. The university-affiliated Lifelong Education Center plays a role in ensuring the right to learn for all citizens as well as fulfilling the social service function and greatly expanding educational opportunities, which is one of the essential functions of universities, by making good use of the excellent transfer material resources of universities. Adults should now seek professional self-identity through retraining, and respond flexibly to various social situations such as increasing roles, expanding responsibility, and uncertainty in the job and employment structure toward professional socialization in the professional world The subject of the study was to collect data by distributing 90 questionnaires to adult learner hair education subjects in G area, and 85 copies were finally used for SPSS 26.0 for Windows analysis, excluding questionnaires with insufficient responses. The survey period was from November 1 to December 27, 2023. First, it was found that adult learners' motivation to participate in hair education has an effect on the lifelong learning happiness index. Second, it was found that adult learners' satisfaction with the program of hair care workers has an effect on the lifelong learning happiness index. Through this study, it is judged that it is necessary to understand what can maximize the high lifelong learning happiness index, and to meet the learning needs of modern people living in the age of 100, increase their potential, help them design a second life, contribute to self-realization and society, and help them have steady, self-directed lifelong learning opportunities.