• Title/Summary/Keyword: 혼합 추출물

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The Acaricidal Effects of Slaked Lime and Plant Extracts on Poultry Red Mites (소석회와 식물 추출물의 닭진드기에 대한 구충 효과)

  • Hong, Eui-Chul;Park, Ki-Tae;Kang, Bo-Seok;Kang, Hwan-Ku;Jeon, Jin-Joo;Kim, Hyun-Soo;Son, Jiseon;Kim, Ji-Hyuk
    • Korean Journal of Poultry Science
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    • v.47 no.4
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    • pp.211-217
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    • 2020
  • This study evaluated calcium hydroxide (slaked lime) and a combination of plant extracts ('natural product'; clove, cinnamon, and saponin; 1:1:1 ratio) as acaricidal control mechanisms for poultry red mites. Red mite susceptibility was evaluated after treatments with 10% slaked lime, 20% slaked lime, and 1% natural product. The duration of the acaricidal effect was also tested at 0, 10, 30, and 60 min after treatment using 20% slaked lime, 1% natural product, or a mixture of both. In the in vitro experiment, the slaked lime treatments were 73.2% (10% slaked lime) and 85.1% (20% slaked lime) effective on red mites. In acaricidal effect of control materials over times, with 20% slaked lime, the acaricidal effect decreased to 50.7% after 30 min, and 12.7% after 60 min (P<0.05). With 1% natural product, there was no acaricidal effect after 30 min (P<0.05). With 20% slaked lime +1% natural product, all of poultry red mites died until 30 min, and 92.9% after 60 min (P<0.05). On the farm, poultry red mites were observed that the number of poultry red mites increased 7,923 from 36 to 45 weeks, but then decreased to 483 after 20% slaked lime plus 1% natural product treatment. These results indicate that combining slaked lime and plant extracts effectively control poultry red mites.

Skin Protection Effect of Grape Pruning Stem Extract on UVB-induced Connective Tissue Injury (포도전정가지 추출물이 UVB로 유도된 결합 조직 손상에 미치는 피부 보호 효과)

  • Kim, Joung-Hee;Kim, Keuk-Jun
    • Journal of Life Science
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    • v.28 no.2
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    • pp.141-147
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    • 2018
  • This study aimed to analyze the contents of rutin, procyanidin B3, quercetin, and kaempferol, known to have antioxidant, anti-inflammatory, and anti-carcinogenic effects, among the polyphenol types contained in grape pruning stem extracts (GPSE). It utilized grape stems discarded after harvest to measure the effects of GPSE on skin moisture, inhibition of skin cell proliferation, and anti-inflammatory activity on the damaged skin of HR-1 mice induced with ultraviolet B (UVB), and to verify the applicability of GPSE as a material for functional food and functional cosmetics. The polyphenol was extracted from grape pruning stems with 80% EtOH, and then the extract was used while storing at $-20^{\circ}C$, after filtering, concentrating, and freeze-drying it. The content of an active ingredient of GPSE was analyzed using high performance liquid chromatography (HPLC). From 53 kg of the grape pruning stem specimen, 2.34 kg of the EtOH fraction extracts were extracted to achieve a 4.42% yield ratio. Analysis of the active ingredients showed 0.28 mg/g of procyanidin B3, 12.81 mg/g of rutin, 0.51 mg/g of quercetin, and 8.24 mg/g of kaempferol. After UVB irradiation on the dermis, to confirm the degree of inhibition of collagen synthesis, we examined the protein expression of MMP-9 using immunohistochemical staining. The results of this study confirm the existence of active polyphenol types, such as rutin, kaempferol, quercetin, and procyanidin B3, in GPSE. Moreover, the study found that GPSE has anti-collagenase effects and it decreases the effects of UV damage on skin barrier function. GPSE is a functional ingredient with a potential for skin protection effects, and it has high utilization potential as an ingredient for functional cosmetics.

Development of satellite precipitation process module based on QGIS (QGIS 기반 위성강수 처리 모듈 개발)

  • Kim, Joo Hun;Kim, Kyeong Tak;Jo, Minhye
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.60-60
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    • 2019
  • OECD 발표에 의하면 물산업 관련 인프라 투자 전망은 전세계 GDP 대비 2010~2020년 약 1.01%에서 2020~2030년 약 1.03%로 확대될 전망으로 다른 통신, 전력, 철도 인프라 투자수요보다 많을 것으로 전망하고 있다(파이넨셜 뉴스, 2013.3.21.). 우리나라는 2005년 베트남 홍강종합개발사업을 시작으로 2015년 기준으로 세계 35개국에 진출하고 있다. 그러나 대부분의 물 산업 진출 대상 국가는 미계측 유역이 많고 지상에서 계측된 수문 자료가 부족한 실정이다. Namgung and Lee(2014)에 의하면 네팔의 수력발전소 건설에 관측된 강우량 자료가 없어 발전소 하류 10km 지점의 유하량 자료를 이용하여 자료의 정확도 검증을 대신하여 적용한 바 있다. 이와 같이 계측자료가 없거나 부족한 지역에 대하여 기상 위성을 이용하여 추정된 강수량 자료가 해당 지역의 강수 특성을 파악하는데 중요한 자료로 이용될 수 있다. 글로벌 위성 기반의 강수량 관측에 대한 역사는 1979년에 IR방법에 의해 위성으로부터 강우자료를 유도하는 개념이 도입된 이후 1987년 다중 채널의 마이크로파(MW) 복사계를 이용한 방법, 이후 두 IR과 MW를 혼합한 방법에서, 1997년 TRMM위성의 PR(Precpipitation Radar)의 레이더를 이용하는 방법, 그리고 2014년 GPM 핵심 위성(GPM Core Observatory)에 탑재된 Dual PR에 의한 방법으로 위성강수의 정확도를 매우 높여가고 있다. 본 연구는 대표적인 위성강수인 IMERG(Integrated MultisatellitE Retrievals for GPM)의 활용성을 높이기 위해 QGIS 기반의 위성강수 전처리 모듈을 개발하는 것을 목적으로 하고 있다. 위성강수를 활용하기 위해서는 위성강수의 정확도 평가가 선행되어야 한다. 본 연구를 통해 2017년 7월 중부지방 및 충청도 지방에 내린 강수자료를 비교한 결과 상관계수가 약 0.7정도로 상관성이 높은 것으로 분석되었고, 2018년 8월 9호 태풍 솔릭(Solik)에 대한 1시간의 시간해상도 분석 결과 상관계수 0.624로 위성강수의 활용성이 있음을 입증하였다. IMERG 위성강수의 활용성을 높이기 위하여 HDF5 포맷의 원시자료를 활용이 용이한 Tiff 로 변환하는 기능에서부터 특정범위 및 특정지점 추출 기능, Resampling 기능 등을 포함하는 전처리 모듈을 개발하였다.

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Hypoglycemic and Hypolipidemic Effects of Jerusalem artichoke Composites in Streptozotocin induced Diabetic Rats (명월초, 여주 및 울금을 포함하는 돼지감자 복합물의 streptozotocin 유발 당뇨쥐에서 혈당강하 및 체내 지질개선에 미치는 영향)

  • Hu, Wen-Si;Lee, Soo-Jung;Pyo, Jae-Ho;Kim, Sung-Hee;Sung, Nak-Ju
    • Journal of Life Science
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    • v.28 no.6
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    • pp.671-680
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    • 2018
  • Hypoglycemic and hypolipidemic effects of Jerusalem artichoke composites (JAP) with extracts of G. procumbens (12.5%), M. charantia (12.5%), and C. longa (12.5%) to H. tuberosus concentrate (JA, 50%) were evaluated in streptozotocin (STZ) induced diabetic rats. Rats were divided into seven groups: normal (Normal), diabetic control (Diabetic), group fed G. procumbens extract (0.5 g/kg bw, D-GPE), group fed JAP (0.5 g/kg bw, D-JAP1; 1.5 g/kg bw, D-JAP2), group fed JA (0.5 g/kg bw, D-JA), and group fed Metformin (0.2 g/kg bw, D-MET) as a positive control. The blood glucose levels over 4 weeks were significantly decreased in the D-JAP2 and D-MET groups compared to the other groups after 3 weeks. The serum insulin level was not significant among the groups fed an experimental diet, but the HOMA-IR value was significantly decreased compared to the diabetic control group. AST and ALT activities in the serum were lowest in D-JAP1. Total lipid and triglyceride contents in the serum decreased in the groups fed an experimental diet, and the HDL-C contents of D-GPE, D-JAP1, and D-JAP2 were significantly increased compared to the diabetic control group. Triglyceride contents in the liver tissue were significantly lower in the D-GPE, D-JAP1, and D-JAP2 groups, and hepatic TBARS content was significantly decreased in the D-JAP1 and D-JAP2 groups compared to the diabetic control group. Hepatic antioxidative enzyme levels, such as SOD, catalase, and GSH-Px, were significantly elevated in groups fed an experimental diet compared to the diabetic control group. Therefore, JAP may be more effective than JA in the human body due to its hypoglycemic and hypolipidemic activities.

Acute toxicity evaluation of maitake mushroom extract and the mixture of maitake and white jelly mushroom extract (잎새버섯(Grifola frondosa) 추출물 및 잎새버섯과 흰목이 (Tremella fuciformis) 혼합추출물의 급성독성시험)

  • Oh, Young-Joo;Park, Kum-Ju;Ha, Hyo-Cheol;Kim, Hyun-Su
    • Journal of Mushroom
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    • v.8 no.3
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    • pp.91-95
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    • 2010
  • The acute toxicity of a crude extract of maitake mushroom(Grifola frondosa practical compound: GFPC) and the mixture of maitake mushroom extract and white jelly mushroom extract(Tremella fuciformis practical compound: TFPC). For acute oral toxicity test, male and female ICR mice were randomly assigned to five groups, consisting of 12 animals each, six males and six females, received either GFPC or the mixture of GFPC and TFPC, at dose of 0, 2,000 and 5,000mg/kg b.w. by orally(10ml/kg b.w.). For the period of 48hr, clinical signs, body weight and food intake were measured. All animals survived during the study and did not show any clinical signs. Food intake was mildly decreased in both GFPC and the mixture of GFPC and TFPC treated groups, however, body weight gain showed no significant difference among the groups. It is suggested that LD50 of GFPC and the mixture of GFPC and TFPC by oral administration was estimated to be over 5,000mg/kg in both sexes of mice. These results conform that GFPC and the mixture of GFPC and TFPC are safe and no toxic at average dietary level.

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Separation of Antioxidant Compounds from Edible Marine Algae (식용 해조류에서 항산화 물질의 분리)

  • Park, Jae-Han;Kang, Kyoo-Chan;Baek, Sang-Bong;Lee, Yoon-Hyung;Rhee, Kyu-Soon
    • Korean Journal of Food Science and Technology
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    • v.23 no.3
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    • pp.256-261
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    • 1991
  • To isolate new antioxidants from twelve edible seaweeds, mixed methanol and chloroform extract of marine algae was fractionated into several subfractions and their antioxidant activities were measured by using AOM and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Especially the aqueous-methanol soluble fractions of brown and red algae showed a considerable antioxidant effect. Their antioxidant activities were stronger than synthetic antioxidants such as BHA, BHT, under the same concentration. Further fractionation of the aqueous-methanol soluble fractions using silica gel column chromatography yielded five subfractions. Among them methanol fraction exhibited high DPPH quenching activities. Also, it was confirmed to be benzene-derivative substances of two compounds by UV, HPLC, GC/MS analysis. Its each molecular weight was about 181, 238. These results suggested the existence of two effective natural antioxidant compounds in three edible marine algae.

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Assessment of the Willingness to Pay for Forest Management in the Upstream for Water Quality Improvement within the Han River Watershed (수질개선을 위한 한강 수계 상류지역 산림관리 지불의사금액 추정)

  • Kim, Dong-Hyun;Kim, Chul-Sang;Lee, Ho-Sang;Park, Kyung-Seok;Mun, Ji-Min;Jeon, Hyon-Sun
    • Journal of Environmental Policy
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    • v.14 no.2
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    • pp.49-72
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    • 2015
  • Forests in the upstream contributed to improve the quality of water resources for the residents downstream. However, upon structural examination of how the Han River Watershed Management Fund was spent, it became apparent that the fund was not spent toward forest management in the upstream. An additional budget must be allocated if the Watershed Management Committee is to contribute to the management of the upstream forests with such awareness. Therefore, the aim of the study was to assess the willingness to pay and to calculate of budget for forest management in the upstream for water quality improvement. Three hundred surveys on watershed beneficiaries were conducted using biased sampling method. The result was analyzed with conditional logit model and mixed logit model. Forest management, a target variable, was found to have statistical significance. Based on this result, the size of the expected budget was estimated to be minimum 20,526 million won to maximum 20,928 million won.

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Characterization of Leaching of Heavy Metal and Formation of Acid Mine Drainage from Coal Mine Tailings (석탄폐석(石炭廢石)으로부터 산성광산배수(酸性鑛山排水)의 생성과 중금속(重金屬)의 용출(溶出) 특성(特性))

  • Lee, Gye-Seung;Song, Young-Jun
    • Resources Recycling
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    • v.19 no.2
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    • pp.54-62
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    • 2010
  • In order to obtain the basic data for recycling of coal mine tailings, the characteristics of coal mine tailings, the formation of AMD from the tailings and the leaching of heavy metal from the tailings were studied. The samples were characterized in terms of chemical composition, mineral composition and content of heavy metal of the tailings were investigated. The effects of time, temperature, content of pyrite, sulfide minerals on the formation of AMD were also investigated. For the leaching tests, the KS method, TCLP method and column test were used. From the investigated data, we can see that the coal mine tailings can be used as aggregate for filling used gallery. We can also see that the formation of AMD from coal mine tailings can be prevented by mixing 4% or more limestone with it.

Inhibitory Effects of PLM-WE1 Formulated from Extract of Phellinus linteus Mycelium against Plant Viruses Infection and Identification of Active Compound (목질진흙버섯(Phellinus linteus) 균사체 추출물 제제 PLM-WE1의 식물 바이러스에 대한 감염억제 효과 및 활성성분의 동정)

  • Kwon, Soon-Bae;Bae, Seon-Hwa;Choi, Jang-Kyung;Lee, Sang-Yong;Kim, Byung-Sup;Kwon, Yong-Soo
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.259-265
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    • 2010
  • Pepper mild mosaic virus(PMMoV) and Cucumber mosaic virus (CMV) are important pathogens in various vegetable crops worldwide. We have found that hot water extract of Phellinus linteus mycelium strongly inhibit PMMoV and CMV infection. Based on these results, the inhibitor named as 'PLM-WE1' formulated from extract of Phellinus linteus mycelium was tested for its inhibitory effects on PMMoV and CMV infection to each local lesion host plant (Nicotiana glutinosa: PMMoV, Chenopodium amaranticolor: CMV). Pretreatment effect of PLM-WE1 against infections of each virus (PMMoV and CMV) to local host plant was measured to be 99.2% to PMMoV and 80.3% to CMV, and its permeability effect was measured to be 45.0% to PMMoV and 41.9% to CMV. Duration of inhibitory activity of PLM-WE1 against PMMoV infection on N. glutinosa was maintained for 3 days at 75% inhibition level and CMV infection on C. amaranticolor maintained for 3 days at 62% inhibition level. Inhibitory effects on systemic host plants of PLM-WE1 were measured to be 75~85% to PMMoV and 75% to CMV. Under electron microscope, PMMoV particles were not denatured or aggregated by mixing PLM-WE1. It is suggested that the mode of action of PLM-WE1 differ from that of inactivation due to the aggregation of viruses. The methanol extract of P. linteus mycelium was sequentially partitioned with haxane, ethyl acetate, BuOH and $H_2O$. The $H_2O$ fraction was showed high activity than the other fractions. The active compound was isolated with a partial acid hydrolysis, fractional precipitation with ethanol. The inhibitory effect of the precipitate isolated from 70% ethanol fraction was 99.1% to PMMoV and 88.0% to CMV. The structure of isolated compound was determined by $^1H$-NMR and $^{13}C$-NMR. This compound was identified as a polysaccharide consisting alpha or beta-glucan.

Sorghum Extract Lowers Lymphatic Absorption of Trans Fat and Cholesterol in Rats (흰쥐에서 수수추출물이 트랜스지방산이 함유된 지방과 콜레스테롤의 흡수에 미치는 영향)

  • Kim, Juyeon;Noh, Sang Kyu;Woo, Koan-Sik;Seo, Myung-Chul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.6
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    • pp.783-788
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    • 2016
  • Excessive intake of trans fats is a risk factor for cardiovascular diseases. Previously, we showed that green tea extract lowers intestinal absorption of cholesterol and elaidic acid in rats. This study was conducted to investigate a possible role for sorghum extract on lymphatic absorption of trans fat and cholesterol in rats. Adult male rats with lymph cannulae were infused at a rate of 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing $146.4{\mu}mol$ of trielaidin, $36.8{\mu}mol$ of trilinoelaidin, $452.0{\mu}mol$ of triolein, $1.0{\mu}Ci$ cholesterol labeled with $^{14}C$ ($^{14}C-cholesterol$), $20.7{\mu}mol$ of cholesterol, and $396.0{\mu}mol$ of Na-taurocholate without or with 100.0 mg of sorghum extract in phosphate-buffered saline buffer (pH 6.4). Lymph was collected hourly for 8 h. No significant difference was noted in lymph flow. However, the lymphatic absorption of elaidic acid and linoelaidic acid for 8 h was significantly lower in rats infused with sorghum extract than in those infused with no sorghum extract. Further, lymphatic absorption of $^{14}C-cholesterol$ was reduced by sorghum extract, which was observed previously. These data indicate that sorghum extract has an inhibitory effect on the intestinal absorption of trans fat and cholesterol. The mechanism(s) by which sorghum extract lowers intestinal absorption of trans fat warrants further study.