• Title/Summary/Keyword: 호기조건

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Nutrient Removal using the Denitrifying Phosphate Accumulating Organisms (dPAOs) and Microbial Community Analysis in Anaerobic-Anoxic Sequencing Batch Reactor (Denitrifying Phosphate Accumulating Organisms (dPAOs)을 이용한 영양소제거 및 반응조내 미생물 분포 조사)

  • 박용근;이진우;이한웅;이수연;최의소
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.113-118
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    • 2002
  • Laboratory experiments were aimed to evaluate the effect of nitrate as a electron acceptor during the biological phosphorus uptake and to investigate the microbial community. Anaerobic-anoxic sequencing batch reactor (SBR) compared the removal behaviour to anaerobic-oxic SBR, both SBRs maintained lower effluent quality with 1.0 mgp/1. Anaerobic-anoxic SBR was able to remove additional 5.0 to 7.0 mg (P+N)/ι than other biological nutrient removal (BM) system. Therefore, it was proposed that the anaerobic-anoxic SBR was more effective at weak sewage. From the results of the maicrobial community analysis, it can be inferred that denitrifying bacteria and polyphosphate accumulating bacteria coexist in anaerobic-anoxic SBR during stable condition for removing the nitrogen and phosphorus. Particularly, it was suggested that the Zoogloea ramigera in the $\beta$-subclass of proteobacteria and the Alcaligenes defragrans of the Rhodocyclus group in the $\beta$-subclass of proteobacteria played a major role for removing the nitrogen and phosphorus as dPAOs (denitrifying phosphate accumulating organisms).

Survival of Campylobacter jejuni under Aerobic Condition (인체장염유발 Campylobacter jejuni의 호기적 조건 하에서의 잔존 양상)

  • Shin, Soon-Young;Kim, Kwang-Yup;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.916-923
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    • 1998
  • To provide more information on the enteric pathogen Campylobacter jejuni in the view of food sanitation, survival characteristics of two strains of C. jejuni in the different conditions were investigated. When $10^7{\;}or{\;}10^3{\;}per{\;}ml$ of C. jejuni cells were inoculated in the supplemented Brucella broth and kept at $42^{\circ}C,{\;}25^{\circ}C{\;}and{\;}5^{\circ}C$ under the static aerobic condition for 7 days, organisms exponentially proliferated to $a{\;}>10^8$, even in the $10^3{\;}per{\;}ml$ inoculated-sample at $42^{\circ}C{\;}for{\;}1{\sim}2{\;}days$ and the considerable level of viability maintained during 7 days. At $5^{\circ}C$, most of the initial level of organisms survived at the early period and only $a{\;}<{\;}0.5-log_{10}$ cells decrease were observed during the 7 days. At $25^{\circ}C$, a remarkable number of C. jejuni declined within $1{\sim}2{\;}days$ and showed undetectable level of cells after 4 days. When sterile milk and minced chicken meat were artifically inoculated with $10^7{\;}per{\;}ml$ of C. jejuni, mostly, a $1-to{\;}2-log_{10}$ count decrease occurred at $42^{\circ}C{\;}and{\;}5^{\circ}C$ while $a{\;}>3{\;}log_{10}$ decrease at $25^{\circ}C$ during 7 days. Unexpectedly, no colonies appeared on the plate inoculated from the minced chicken meat sample kept at $42^{\circ}C$ after 4 days. The results suggest that C. jejuni contaminated to food can survive at the refrigeration temperature whereas they are sensitive to at the room temperature. Also, it is shown that the growth of C. jejuni at the optimal temperature may vary to the food sources.

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천이노심 DNBR 벌점 평가방법 개선

  • 김강훈;전병순;박응준
    • Proceedings of the Korean Nuclear Society Conference
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    • 1995.10a
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    • pp.389-395
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    • 1995
  • 기존의 천이노심 DNBR 벌점 평가 방법을 개선하여 불확실도를 줄이고 신뢰도를 향상시키며, 적용범위를 확대함으로써 보다 실제적인 DNBR 벌점 평가 방법을 제시하고자 하였다. 이를 위하여 영광 1호기 JDFA-V5H 의 천이노심을 대상으로 하는 일련의 분석이 수행되었다. 먼저 균일노심과 천이노심을 모형화 한 기준 제어군에서의 상대적인 물성치의 변화와 축방향에서의 DNBR 거동을 분석하였고 이에 따른 최소 DNBR 의 상대적 차이로부터 최대 럴점 조건 및 벌점이 적용되는 집합체를 선정하였다. 변수 민감도 분석 결과, 최대 벌점 조건은 과출력 (120% 출력), 고압 (2420 psia) 그리고 상부노심에서 상대출력이 많은 축방향 출력 분포를 갖는 조건이 선정되었고 천이노심 벌점은 V5H에만 부과된다. 천이노심 DNBR 벌점은 배열 민감도 분석을 통하여 노심내 V5H 분율의 함수로 표현됨을 알 수 있었으며, 기존의 보수적인 방법론에 비해 최소 3% 이상의 천이노심 벌점이 감소되는것으로 나타나 추가적인 여유도의 확보로 인한 설계의 탄력성을 기대할 수 있다. 이 결과는 IFM이 존재하는 원전연료 집합체 상부에 대하여 노심의 V5H 분율이 0.02 부터 1.0 까지의 정상 및 과도상태 노심에 대하여 적용할 수 있다.

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핵연료 봉의 Fretting Wear어 대한 열수력학적 원인 분석

  • 김상녕;정성엽
    • Proceedings of the Korean Nuclear Society Conference
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    • 1998.05a
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    • pp.496-501
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    • 1998
  • 최근 국내의 PWR 발전소에서는 유체유발진동에 의한 핵연료의 Fretting Wear가 많이 발생하였다. 이는 Baffle Jetting이나 그 밖의 요인도 있을 수 있으나 핵연료의 장주기화, 높은 열적여유도등의 설계요건을 만족하기 위한 노심 내의 유동조건 변화에 기인한다. 특히 고리 2호기에서 발생한 핵연료 손상 중 15%정도가 유체유발진동으로 추정되고 있다. 따라서 본 연구는 손상 핵연료의 노심내 위치, 부위, 유동조건 등으로 부터 유체유발진동의 주요 손상 원인을 규명하는데 있다. 이를 위해 핵연료 집합체에서 발생할 수 있는 유체유발진동 메카니즘의 특징과 유동조건, 손상 핵연료의 노심내 위치, 파손 부위, 집합체와 지지격자의 기하학적 형태를 고려한 유동 방향 등을 연관 분석 결과 파손을 일으키는 주요원인을 단일 집합체 내에서 발생되는 Vortex Shedding과 인접한 집합체 사이에서 발생되는 Fluidelastic Instability의 중복효과로 규명하였다 또한 최근 핵연료 설계에 도입된 Mixing Vane의 효과가 과도하여 핵연료 손상을 일으키는 가설을 정립하였다.

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Reduction of the Nitrogen in the Secondary Effluent by the Hybrid Sequential Aerobic-Anoxic Natural System (자연현상을 이용한 질산화-탈질공정에 의한 하수처리장 유출수의 질소제거)

  • Kim, Young-Chul;Chung, Paul-Gene;An, Ik-Sung
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.3
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    • pp.323-329
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    • 2005
  • In this paper, performance of a hybrid sequential aerobic-anaerobic natural system was investigated. Continuous aerobic and anoxic conditions were created by alternatively placing waste stabilization pond (WSP) and wale. hyacinth pond (WHP). Two pilot-scale treatment lines were built and operated; The first consists of WSP integrated with WHP and the second of WSP connected with Dark Pond(DP), namely control system ponds which were used to examine the effects of water hyacinth on nitrification and de-nitrification. The overall performance in nitrogen was 86% reduction in WSP-WHP and 36% in WSP-control pond system. Nitrogen was mostly removed by nitrification and de-nitrification which simultaneously occurred in the same water hyacinth ponds. For the de-nitrification, benthic layer was found out to be adequate support as a carbon source. In addition, WSP-WHP system was very effective in reducing phosphorus. Overall P removal efficiency in WSP-WHP is 81%, while it is only 16% in WSP-control. difference in phosphorus reduction between those two systems is thought to be caused by the plants and probably their roots producing extra-cellular materials, but these aspects need to be further studied.

Characteristics of Stabilization of Excavated Solid Wastes by Aerobic and Anaerobic Landfilling (호기 및 혐기매립에 의한 굴착폐기물의 안정화 특성 연구)

  • Park, Jin-Kyu;Oh, Dong Ik;Lee, Nam-Hoon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.3
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    • pp.76-85
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    • 2004
  • Anaerobic decomposition of municipal solid waste (MSW) had potential adverse impacts such as the production of methane and long-term post closure on human health and the environment. It was demonstrated that aerobic degradation of MSW resulted in the reduction of a methane yield and the enhancement of stabilization of MSW. Excavated solid wastes were both aerobically and anaerobically treated in order to evaluate the effects of air injection on the stabilization of landfill site. The municipal solid waste (MSW) samples were excavated from a 10-year old landfill (operation period: 1991. 11~1994. 11), Jeonju, Korea. Excavated municipal solid wastes are primarily composed of soils and vinyl/plastics. For the two aerobic simulated lysimeters, the levels of $O_2$ ranged 1.6~23.1% and the levels of $CO_2$ ranged 1.5~15.1%, which confirmed the aerobic decomposition. Aeration did prevent methane formation. For the anaerobic simulated lysimeter, the $CO_2$ rose as $O_2$ was consumed and low levels of CH4 were produced. The pH levels ranged from 7.7 to 8.9 for anaerobic lysimeter and from 7.3 to 8.5 for aerobic lysimeters. As expected, aerobic treatment proved to enhance the removal of biodegradable materials in the excavated solid wastes when monitoring the concentration of BOD, COD, $NH_4-N$, and $NO_3-N$ in the leachate.

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A Study on the Distribution Characteristics of Nitrite Oxidizing Bacteria in Wastewater Nitrification Systems (폐수 질산화 시스템에서 아질산 산화 미생물의 분포 특성 연구)

  • Kim, Sun-Hee;Kim, Dong-Jin;Yoo, Ik-Keun;Cha, Gi-Cheol
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.10
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    • pp.1024-1030
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    • 2006
  • Genus Nitrospira and Nitrobacter species are the key nitrite-oxidizing bacteria(NOB) in nitrifying wastewater treatment plants. It has been hypothesized that genus Nitrospira are K-strategists(low $K_6$ value) that can exploit low amounts of nitrite more efficiently than Nitrobacter. In contrast, Nitrobacter species are r-strategists(high $V_{max}$) that can grow faster than Nitrospira. It has also been known that the availability of organic compounds and dissolved oxygen as well as nitrite affects the distribution of NOB. In this study, we determined the distribution and competition of NOB in wastewater nitrification systems where nitrite, organic compounds, and dissolved oxygen concentrations were compositively varied. For the purpose, several compounds of the laboratory-scale nitrificaiion bioreactor and full-scale $A_2O$ wastewater treatment plant and their distribution of NOB were analyzed and compared. The analysis showed that Nitrobacter was the dominant NOB in nitrification bioreactor where average nitrite was maintained at 5 mg-N/L with very low organic concentration in aerobic condition, whereas Nitrospira was the dominant NOB in full-scale $A_2O$ plant where nitrite was maintained very low and organic compounds were maintained relatively high in alternating aerobic-anoxic condition. The result indicates that nitrite concentration is more critical factor than organics and dissolved oxygen which determines the dominant NOB in nitrification system and it is confirmed that Nitrospira and Nitrobacter showed the characteristics of r-strategist and K-strategist, respectively.

Bacterial Flora of the Intestine in Normal Captive Oriental White Storks (정상적인 사육 황새의 장내 세균총)

  • Han, Jae-Ik;Jang, Hye-Jin;Lee, Sook-Jin;Kang, Hyo-Min;Kim, Su-Kyung;Park, Shi-Ryoung;Na, Ki-Jeong
    • Journal of Veterinary Clinics
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    • v.28 no.5
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    • pp.516-518
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    • 2011
  • A survey was conducted to examine the normal intestinal bacterial flora of captive Oriental white storks (Ciconia boyciana) maintained at the Korea Institute of Oriental White Stork Rehabilitation Research, Cheongwon, South Korea. From the cloaca of 44 healthy storks, 44 fecal samples were collected and cultured under aerobic and anaerobic conditions. The 16S ribosomal RNA gene sequences and the heat shock protein 60 gene were cloned and sequenced for bacterial identification. Under aerobic conditions, Enterococcus faecalis, Escherichia coli, Bacillus spp., Enterococcus avium, Enterococcus gallinarum, Pseudomonas spp., Alcaligenes spp., Enterobacter spp., Corynebacterium spp., and Proteus mirabilis were identified. Under anaerobic conditions, E. coli, Clostridium tertium, En. faecalis, and P. mirabilis were identified. E. coli, En. faecalis, or both were isolated from all samples. These results will add to the information available on this stork species and help for the interpretation of fecal culture results.

Evaluation of DOM Variations and Reduction Effects in Bioreation Artificial Wetland (생물반응 인공습지 내 DOM 변동 및 저감효과 평가)

  • Joo, Kwangjin;Lee, Jongjun;Kim, Tea-Kyung;Choi, Isong;Chang, Kwang-hyeon;Joo, Jinchul;Oh, Jongmin
    • Journal of Environmental Impact Assessment
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    • v.27 no.6
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    • pp.582-594
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    • 2018
  • In this study, the vertical and horizontal flow wetlands were combined in series to create conditions for flow in the exhalation and anaerobic state with the aim of monitoring the variability and reduction of dissolved organic matterin the bio-reactive artificial wetlands, and the performance assessment was conducted as acrylic reaction groups by designing artificial wetlands that filled the functionalresiduals. In case of artificial wetlands in vertical and horizontal planes, the concentration of dissolved oxygen (DO) in the reaction tank was measured as 2.7 mg/L in the vertical flow wetlands under exhalation, and N.D. in the horizontal flow artificial wetlands under anaerobic conditions. The test was carried out by changing the operation time to 140 min, 80 min, and 60 min. The test was conducted with the same natural operation time of 20 min depending on the operation time. All hours of operation were shown to be due to microbial activity. In 3D-EEM, it was found that the longer the driving time was taken, the more reduction the organic compounds in the areas of insoluble human resources, III and V. Further research on the mechanism analysis of future reduction effects is expected to be carried out, but the findings are expected to contribute to the development of technologies for reducing obfuscated substances using artificial wetlands in the future.

CE형 원전연료 부품의 국산 시제품에 대한 구조강도 및 특성시험

  • 송기남;서정민
    • Proceedings of the Korean Nuclear Society Conference
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    • 1996.05c
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    • pp.33-38
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    • 1996
  • 영광 3,4호기용 원전연료에 사용되는 구조용 부품들 중에서 coil형 Holddown spring을 포함한 Upper end fitting assembly, Lower end fitting 등을 국내에서 제조할 수 있는 제조공정이 대우 정밀(주)에서 개발되었다. 이 공정에서 제조한 시제품들에 대하여 기계/구조적인 설계 관점에서 요구되는 제 조건들의 만족 여부를 구조강도 및 특성시험을 통하여 검증하였다. 검증시험 결과 주조제품인 Holddown plate 및 Lower end fitting은 주조물에서 요구되는 제기준과 기계/구조적인 설계 관건에서의 구조적 강도요건을 만족하고 있었고 coil형 Holddown spring의 특성이 설계 요구범위내에 있음이 확인됨으로서 향후 생산될 원전연료에는 금번에 개발된 공정으로 제조될 부품들이 사용필 수 있으리라 전망된다.

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