Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Nutrient Removal using the Denitrifying Phosphate Accumulating Organisms (dPAOs) and Microbial Community Analysis in Anaerobic-Anoxic Sequencing Batch Reactor

Denitrifying Phosphate Accumulating Organisms (dPAOs)을 이용한 영양소제거 및 반응조내 미생물 분포 조사

  • 박용근 (고려대학교 생명공학원) ;
  • 이진우 (고려대학교 환경시스템 공학과) ;
  • 이한웅 (고려대학교 생명공학원) ;
  • 이수연 (고려대학교 생명공학원) ;
  • 최의소 (고려대학교 토목·환경공학과)
  • Published : 2002.06.01
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Abstract

Laboratory experiments were aimed to evaluate the effect of nitrate as a electron acceptor during the biological phosphorus uptake and to investigate the microbial community. Anaerobic-anoxic sequencing batch reactor (SBR) compared the removal behaviour to anaerobic-oxic SBR, both SBRs maintained lower effluent quality with 1.0 mgp/1. Anaerobic-anoxic SBR was able to remove additional 5.0 to 7.0 mg (P+N)/ι than other biological nutrient removal (BM) system. Therefore, it was proposed that the anaerobic-anoxic SBR was more effective at weak sewage. From the results of the maicrobial community analysis, it can be inferred that denitrifying bacteria and polyphosphate accumulating bacteria coexist in anaerobic-anoxic SBR during stable condition for removing the nitrogen and phosphorus. Particularly, it was suggested that the Zoogloea ramigera in the $\beta$-subclass of proteobacteria and the Alcaligenes defragrans of the Rhodocyclus group in the $\beta$-subclass of proteobacteria played a major role for removing the nitrogen and phosphorus as dPAOs (denitrifying phosphate accumulating organisms).

혐기-무산소조건으로 구성된 회분식 반응조에서 질산염을 이용하여 인(P)도 동시에 제거될 수 있는 가능성을 알아보기 위해서 인의 제거 양상을 혐기-호기조건의 반응조와 비교하여 조사하였고, 질산염과 인을 동시에 제거하는 미생물분포를 분석하였다. 그 결과 비교적 낮은 농도의 유기물이 적용되었을 때(평균 CODcr=130mg/ι)두 반응조 모두 인이 효과적으로 제거되었으며 반응조내의 최종 인의 농도를 1 mg P/L. 이하로 유지하였다. 특히, 질산염을 전자수용체로 이용한 혐기-무산소조건의 반응조는 기존의 영양소제거 시스템과 비교하여 5-7 mg (P+N)/ι의 영양소를 추가적으로 제거하여 유기물의 효과적 인 이용이 가능한 것으로 판명되었다. 혐기-무산조 조건의 방응조내 미생물 분포를 조사 한 결과 질소원을 제거하는 미생물군(denitrifying bacteria)과 인을 제거하는 미생물군(polyphosphate accumulating bacteria)이 함께 존재하고 있음이 밝혀졌고, 이들 중 $\beta$-proteobacteria에 속하는 Zoogloea ramigera와 Rhodocyclus에 포함되는Alcaligenes defragrans 등은 탈질능력 이 있으면서 anoxic상태에서 인을 동시에 축적할 수 있는 탈질-탈인균주(denitrifying phosphate accumulating organisms; dPAO)로 조사되었다.

Keywords

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