Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Identification of Bacteria Lysing Anabaena cylindrica

Anabaena cylindrica 분해세균의 분리 및 동정

  • Choi, Yong-Keel (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration) ;
  • Hong, Yup (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration) ;
  • Shin, Kyu-Chul (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration) ;
  • Kim, Min-Seong (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration) ;
  • Han, Myung-Soo (Department of Life Science, Environmental, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
  • 최영길 (한양대학교 자연과학대학 생명과학과, 국가지정 물환경생태복원연구실) ;
  • 홍엽 (한양대학교 자연과학대학 생명과학과, 국가지정 물환경생태복원연구실) ;
  • 신규철 (한양대학교 자연과학대학 생명과학과, 국가지정 물환경생태복원연구실) ;
  • 김민성 (한양대학교 자연과학대학 생명과학과, 국가지정 물환경생태복원연구실) ;
  • 한명수 (한양대학교 자연과학대학 생명과학과, 환경과학과 , 국가지정 물환경생태복원연구실)
  • Published : 2002.06.01
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Abstract

To isolate the bacteria lysing cyanobacteria, the sediment samples were collected from Dochang and Pal'tang Reservoir and Seokchon Lake. Each sample was smeared on the Anabaena cylindrica lawn and incubated in light chamber for 11 days. Bacteria having cyanobacteria-Iysing activity were isolated from the samples of Seokchon reservoir. Confirmation of cyanobacteria-Iysing activity was carried out to measure chlorophyll a and bacterial cell counting in mixed culture of Anabaena cylindrica and bacteria. Lysis was detected when extracellular meterials was added to the Anabaena cylindrica culture. The isolate was identified by analysis based on 16S rDNA sequence and morphological and physiological properties. The bacterial strain was taxonomically studied by the phylogenetic analysis based on 165 rDNA sequence. This strain was identified as a member of the genus Bacillus and designated as Bacillus sp. CHS1.

남조류 분해세균을 분리하기 위하여 도창, 팔당 저수지와 석촌 호수로부터 저층시료를 채취하였다. Anabaena cylindrica lawn에 각각의 시료를 도말한 후 11 일간 배양하였고 석촌 시료를 접중한 Anabaena cylindrica lawn에서 Anabaena cylindrica에 분해능을 가지는 세균을 분리하였다. 분해능 화인은 남조류와 분해세균을 혼합한 후 chlorophyll a간의 측정과 분리세균의 cell counting방법으로 확인하였고, 세포외 물질이 Anabaena cylindrica를 분해하는 것을 확인하였다. 분리균주는 16S rDNA 염기서열 분석과 형태학적, 생리학적 특징을 기초로 하여 동정하였다. 분리균주의 165 rDNA염기서열을 기초로한 분자계통학적 분석을 통하여 Bacillus속에 포함하는 계통학적 그룹에 속한다는 것을 확인하였고, Bacillus sp. CHSl으로 명하였다.

Keywords

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