• Title/Summary/Keyword: 형광 곡선

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Bioremediation on the Benthic Layer in Polluted Inner Bay by Promotion of Microphytobenthos Growth Using Light Emitting Diode (LED) 1. Effects of irradiance and wavelength on the growth of benthic diatom, Nitzschia sp. (발광다이오드(LED)를 이용한 저서미세조류의 성장촉진에 의한 오염해역 저질환경개선 1. 저서규조류 Nitzschia sp. 성장에 영향을 미치는 광량과 파장)

  • Oh, Seok-Jin;Park, Dal-Soo;Yang, Han-Soeb;Yoon, Yang-Ho;Honjo, Tsuneo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.2
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    • pp.93-101
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    • 2007
  • In order for bioremediate the benthic layer in polluted inner Bay, the effects of irradiance and wave-length irradiated from light emission diode (LED) on the growth of benthic diatom Nitzschia sp. (Hakozaki Bay strain of Japan) were investigated. The Nitzschia sp. was cultured under blue LED (450 nm), yellow LED (590 nm), red LED (650 nm) and fluorescent lamp (mixed wavelengths). At $25^{\circ}C$ and 30 psu, the growth of Nitzschia sp. showed its peak at $20\;{\mu}mol\;m^{-2}\;s^{-1}$ (blue LED) and $40\;{\mu}mol\;m^{-2}\;s^{-1}$ (fluorescent lamp), and was inhibited at the irradiance higher than that irradiance. Nitzschia sp. in yellow LED and red LED is fitted by a rectangular hyperbolic curve because no photoinhibition was observed under maximum irradiance used in this study. The irradiance-growth curves were described as ${\mu}=-0.46{\exp}(1-I/6.32)+0.46-0.00043I,\;(r^2=0.98)$ under blue LED, ${\mu}=0.42(I+7.87)/(I+58.9),\;(r^2=0.99)$ under yellow LED, ${\mu}=0.39(I+3.39)/(I+21.6),\;(r^2=0.94)$ under red LED, ${\mu}=-0.38{\exp}(1-I/7.23)+0.38-0.00016I,\;(r^2=0.96)$ under fluorescent lamp. Maximum specific growth rate of blue LED, yellow LED, red LED and fluorescent lamp was $0.44\;day^{-1},\;0.42\;day^{-1},\;0.39\;day^{-1}$ and $0.37\;day^{-1}$, respectively. The absorption coefficient ($a_{ph}$) of Nitzschia sp. was similar under all the wavelengths (400 nm-700 nm), although maximum $a_{ph}$ was $0.0224\;m^2\;mg\;chi.\;{\alpha}^{-1}$ in 472 nm and $0.0179\;m^2\;mg\;chi.\;{\alpha}^{-1}$) in 663 nm. The results may indicate the possibility of environmental improvement around the benthic layer in polluted coastal area because microphytobenthos growth is stimulated by means of irradiated blue LED at the benthic boundary layer during both autumn and winter, and yellow LED, which might have been suppressed growth of harmful algae, at the layer during both spring and summer.

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Measurement of picosecond laser pulsewidth and pulseshape by two-photon fluorescence and noncolloinear type I second harmonic generation method (이광자 형광법과 비공선 일종 이차고조파법에 의한 피코초 레이저 펄스폭과 펄스형 측정)

  • 한기호;박종락;이재용;김현수;엄기영;변재오;공흥진
    • Korean Journal of Optics and Photonics
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    • v.7 no.3
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    • pp.251-259
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    • 1996
  • Two-Photon Fluorescence (TPF) experiment measures temporal width of an amplified short laser pulse which has passed through a four-pass Nd: glass amplifier, after selecting a single pulse from pulse train Q-switched and mode-locked(QSML) in Nd:YLF master oscillator. Determination of pulsewidth and pulseshape was also made with detection of autocorrelation trace of CW mode-locked pulse train by using noncollinear type I Second Harmonic Generation (SHG) method. The observed TPF track showed various patterns, depending on pulse-selecting position in QSML pulse train. That is, autocorrelation of a pulse extracted at front of the train displayed smooth pulse shape, while one from the trailing part of the train created many sharp spikes and substructure in the pulse. By TPF method, pulsewidth was measured to be 44.4 ps with contrast ratio of 2.86 which enabled us to find out energy fraction of a pulse to total energy, (sum of pulse and background); we obtain the value of 0.62. Pulsewidth of 46.6ps was also acquired in another SHG experiment with the help of only mode-locked pulse train. On the other hand, we confirmed that shape of the pulse is close to $sech^2$ one as a result of fitting the SHG autocorrelation signal with various functions. With simulation using this $sech^2$ type of pulse, pulsewidth reduction of the beam, having passed through four-pass amplifier, was also verified.

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Hydrogen Production by Purple Sulfur Bacteria, Thiocapsa roseopersicina in Photoheterotrophic Culture Condition (홍색유황세균, Thiocapsa roseopersicina의 photoheterotrophic 조건에서의 수소생산)

  • Kim, Mi-Sun;In, Sun-Kyoung;Baek, Jin-Sook;Lee, Jeong-K.
    • KSBB Journal
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    • v.20 no.6
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    • pp.413-417
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    • 2005
  • The purple sulfur phototrophic bacterium, Thiocapsa roseopersicina NCIB 8347 has been studied on hydrogen production and cell growth under different culture conditions, such as light source, light intensity, and growth temperature. T. roseopersicina showed maximum cell growth of 1.38 and 1.42 g-DCW/L under 7.5-10 klux of halogen and fluorescent light, respectively, and produced maximum amount of hydrogen with values of 0.90 and 0.48 $mL-H_2/mg$-DCW under the irradiation of 10 klux of halogen and fluorescent light, respectively. The optimum growth temperature for hydrogen production was $26^{\circ}C$, and hydrogen production rate was lowered over $30^{\circ}C$. When T. roseopersicina was grown photoheterotrophically under irradiation of 8-9 klux of halogen lamp, the generation time was 4.2 hr. The strains started producing hydrgen from the middle of the logarithmic growth phase and continued until succinate concentration leveled out.

A study on the development of SRM for XRF analysis of PZT[$Pb(ZrTi)O_3$] (PZT[$Pb(ZrTi)O_3$]의 XRF 분석용 SRM 개발에 관한 연구)

  • Kim, Young Man;Jeong, Chan Yee;Lim, Chang Ho;Choi, Beom Suk
    • Analytical Science and Technology
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    • v.10 no.6
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    • pp.439-444
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    • 1997
  • Twelve kinds (1set) standard materials of chemical ingredients of lead zirconate titanate[$Pb(ZrTi)O_3$] have been developed in order to determine fast and accurate measurement of X-ray fluorescence spectrometry. Especially, we used diluted(ahout sixteen times) filling compound($Li_2B_4O_7/LiBO_2=4/1$) to consider removal effect of matrix, storage convenience, and homogenous characteristics. As a result from the four different laboratories, we obtained extremly good agreement about the standard curve on twelve standard materials which containing eleven elements, PbO, $ZrO_2$, $TiO_2$, SrO, $WO_3$, $La_2O_3$, $Cr_2O_3$, MgO, $Nb_2O_5$, and $MnO_2$. The correlation factor of standard curve was over 0.998. However, ZnO has relatively low correlation factor, 0.977, because the concentration was 10ppm lower than other original materials. This analysis reveals that ZnO has shown the poor linearity as well as low fluorescence intensity. In present work, XRF standard materials are useful for determining a rapid and accurate results for major and minor elements concentration among PZT.

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광량과 온도 변화에 따른 고추(Capsicum annuum L.) 잎 광계 II의 광억제

  • 홍영남
    • Journal of Plant Biology
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    • v.38 no.4
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    • pp.373-380
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    • 1995
  • Photoinhibition of photosystem (PS) n was induced in primary leaves of 25 day-old peppers grown $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1},\;at\;25^{\circ}C$. The modulation of PSII functionality in vivo was induced by varying both irradiance ($0-3000\;{\mu}molm^{-2}{\cdot}s^{-1}$) and duration (0-70 min) of light treatment. The functionality of PSII was investigated in terms of photochemical efficiency of PSII (Fv/Fm) and quantum yield of $O_2$ evolution, and expressed as a function of photon exposure [$mol\;photons{\cdot}m^{-2}$, the product of irradiance and duration of light treatment (Bell and Rose, 1981)]. Contrary to the linear decline of Fv/Fm ratio showing 50% decreases by absorption of $10\;mol\;photons{\cdot}m^{-2$, quantum yield of $O_2$ evolution decreased biphasically with increasing photon exposure, showing 50% decreases by absorption of $5.5\;mol\;photons{\cdot}m^{-2}$. Treatment of low temperature at $15^{\circ}C$ for 30 min alone did not affect the functionality of PSII, but high temperature ($45^{\circ}C$) significantly inactivated PSII activity. However, when Jeaves of pepper were subjected to low or high temperature in the presence of light, PSII was substantially photoinactivated. These results suggest the presence of different photoinhibitory mechanisms at low and high temperature.rature.

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Growth and Physiological Characteristics of Containerized Seedlings of Sageretia thea at Different Fertilization Treatments (시비처리에 따른 상동나무 용기묘의 생장 및 생리특성)

  • Eo, Hyun Ji;Son, Yong Hwan;Park, Sung Hyuk;Park, Gwang Hun;Lee, Kyeong Cheol;Son, Ho Jun
    • Journal of Korean Society of Forest Science
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    • v.110 no.2
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    • pp.189-197
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    • 2021
  • This study aims to optimize the appropriate concentration of fertilizers for Sageretia thea by analyzing growth performances (height and root collar diameter) and physiological characteristics (photosynthesis, chlorophyll contents, and chlorophyll fluorescence reaction). As fertilizer concentration was increased to 1.5 g·L-1, growth increased, but it decreased at 2.0 g·L-1 treatment. Root collar diameter growth was reduced because of higher fertilizer concentrations. Photosynthesis reactions showed the highest CO2 reaction curves, maximum photosynthesis rate, and maximum carboxylation rate in the 1.5 g·L-1 fertilizer treatment. The chlorophyll fluorescence reaction and SPAD values revealed that fertilizer treatment improves photosynthesis efficiency and robustness compared with untreated control. Therefore, the appropriate fertilizer concentration for producing good seedling quality of Sageretia thea is 1.0~1.5 g·L-1.

Magnetic Properties of Basic Rock in the Seaside Area of Ulleung Island (울릉도 해안지역 염기성암의 자기적 성질 연구)

  • Yoon, In-Seop
    • Journal of the Korean Magnetics Society
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    • v.20 no.4
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    • pp.153-159
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    • 2010
  • Magnetic properties of basic rocks samples distributed in the seaside area of Ulleung island were investigated by means of X-ray fluorescence spectroscopy (XRF), M$\ddot{o}$ssbauer spectroscopy and SQUID magnetometer. We found that samples were typical basic rock which had the total amount of iron compounds varies from 10.6 wt% to 14.5 wt% depending on the different regions by XRF. The M$\ddot{o}$ssbauer spectra and magnetic hysteresis behaviors of the samples show ferromagnetic properties due to hematite. Also, temperature dependences of magnetization at applied magnetic field of 1 T for samples show magnetic transitions at 41 K~60 K.

Scintillation Properties of Eu2+ ions doped LaCl3 Crystal (Eu2+ 이온을 도핑한 LaCl3 결정의 섬광 특성)

  • Kim, Sung-Hwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.2
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    • pp.600-604
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    • 2011
  • In this paper, the scintillation properties of $LaCl_3:Eu^{2+}$ crystal were investigated as new scintillator. This scintillation material was grown by a Czochralski method. $LaCl_3:Eu^{2+}$ was determined to have a hexagonal $P_63$/m space group with cell parameters a = b = $7.48{\AA}$, c = $4.37{\AA}$. Under 335 nm UV excitation, the crystal shows a broad emission band between 370 nm and 640 nm wavelength range, peaking at 430 nm. At room temperature, the crystal exhibits one exponential decay time component. The component of scintillation time profile of the crystal emission decays with a $2.82{\pm}0.72{\mu}s$ time constant. The energy resolution of the crystal was measured to be 8.8% (FWHM) for $^{137}Cs$ 662 keV ${\gamma}$-rays.

Dose Calculation of Heterogeneous Lung Tissue on 6MV X-ray Therapy (6MV X-선에 의한 폐조직의 심부선량변화와 임상응용)

  • 이경자;장승희;추성실
    • Progress in Medical Physics
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    • v.9 no.4
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    • pp.247-257
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    • 1998
  • For effective radiotherapy, it should always be considered that calculation of different dose distribution in heterogenous tissue is important particularly on lung which has low density and large volume. To take precise dose distribution of 6MV X-ray in the thoracic cage, the authors had made a tissue equivalent phantom for thorax, measured dose distribution by thermoluminescent dosimeter and mm dosimeter, and derived methmetical equation coincided with provided theoretical formula. In comparision with isodose curve on case of homogeneous soft tissue, dose of heterogeneous lung tissue had been shown increase about 4% per cm depth on one and multiportal field, less than 15% difference on rotation field for esophagus, and around 20% difference on rotation field for lung according to the degree of rotation angle that must be corrected by dose compensation.

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X-Ray Spectrometric Determination of $Ta_2O_5,\;Nb_2O_5$ and $ZrO_2$in Tin-Slag Samples by Double Dilution Technique (주석-슬랙중 $Ta_2O_5,\;Nb_2O_5$$ZrO_2$의 이중희석법을 이용한 X-선 분광분석)

  • Young-Sang Kim;Kee-Chae Park
    • Journal of the Korean Chemical Society
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    • v.28 no.5
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    • pp.302-308
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    • 1984
  • X-ray spectrometric determination of $Ta_2O_54, $Nb_2O_5$ and $ZrO_2$ in tin-slag samples using the double dilution technique was conducted. Tin-slag samples and one synthesized standard sample having similar composition to the samples were diluted with anhydrous $Li_2B_4O_7$ at the level of 1.00, 2.00 and 3.00% of the sample content, respectively. The diluted samples were fused at $1,150^{\circ}C$ for 30 minutes, and these glass beads were finely ground and pelletized. With the X-ray intensities measured, analytical results were calculated by the double dilution equation. The results agreed to the reference values obtained by the standard calibration method and the simple dilution technique within allowable error range and were reproducible.

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