• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1130-1133
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• 2002

Domoic acid, and amnesic shellfish poison, is a neurotoxin frequently found in shellfishes. Guidance level for the consumable shellfish has been established as $20\;{\mu}g$ domoic acid/g by Health and Welfare Canada and U.S. FDA. Domoic acid is produced by pennate diatom, a Nitzschia pungens f. multiseries ingested by the shellfish. Content of domoic acid in shellfish samples collected along the Korean shoreline from May to December of 1999 was analyzed. The collection included 1 Gastropoda (Murex shell) and 11 Bivalvias (oyster, little neck clam, orient hard clam, venus clam, surf clam, ark shell, hard-shelled mussel, pen shell, jack-knife clam, pink butterfly shell, and granulated ark shell). Samples were homogenized, extracted with 50% methanol, filtered, and analyzed by reversed-phase liquid chromatography at 242 nm with mobile phase consisting of 10% acetonitrile and 0.1% trifluoroacetic acid. Recovery of the HPLC analysis was 95.80% (${\pm}1.09$). All tested samples showed no domoic acid at the detection limit of 50 ng/g.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.71-81
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• 2012

This study was conducted to get basic information about making windbreak forest around Saemanguem reclaimed lands by analyzing the vegetation structure of 8 windbreak forests along the west coast of Korea and considering the methods of structural improvement. The total plants existing in the study areas were 25 families 40genus 43 species, and every tree layer consisted of only one tree species, Pinus thunbergii. There were 2 windbreak stands consisted of a species, and 2 consisted of multiple layer with a species. While, 4 windbreak stands consist of various species with several species. To make ecological system and functions of windbreak forests stabler, TL, STL and SL should be together in the vertical point, moreover, TL should be appeared at the in side and SL should be planted at the out side in the horizontal point. It is considered that mixed stand forest might be suitable for increasing the windbreak forest's functions and decreasing damage caused by disease and insects.

• Ecology and Resilient Infrastructure
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• v.8 no.2
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• pp.97-111
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• 2021

This study was conducted to develop a habitat type classification system and its map based on the ecological characteristics of species, spatial type, vegetation, topography, and geological conditions preferred by species. To evaluate the relationships between species and their habitats in Korean national parks, we prepared a classification standard table for systematic classification of habitat types. This classification system divides habitats into 6 low-level and 59 mid-level ecological classes based on habitat structure. The mid-level system divided forest ecosystems into 20 subtypes, stream and wetland ecosystems into 8 types, coastal ecosystems into 7 types, arable land into 6 types, development land into 9 types, and 1 type of marine ecosystem. A habitat classification map was drawn utilizing square images, detailed vegetation maps, and forest stand maps, based on the above habitat classification system, and it covered 1,461 plots spanning 21 national parks. The habitat classification system and survey protocol, which consider domestic habitat conditions, should be further developed and applied to habitat assessment, to enhance the utility of this study.

• Journal of Life Science
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• v.29 no.3
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• pp.287-294
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• 2019

Calystegia soldanella is distributed in coastal sand dunes and has high environmental adaptability; it is also known to be effective for anti-oxidant, anti-pyretic, anti-septic, and diuretic action. This study investigated the effect of crude extracts and organic solvent fractions of C. soldanella on MMP-2 and MMP-9 expression, MMP activity, and cell mobility in phorbol-12-myristate-13-acetate (PMA)-induced fibrosarcoma HT-1080 cells. C. soldanella was twice extracted, once with methylene chloride (MC) and once with methanol (MeOH). After the MC and MeOH extracts were combined, their suppressive effects on MMP-2 and MMP-9 expression, MMP enzymatic activity, and gene and protein expression were measured by gelatin zymography, enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and western blot method. Cell mobility for the HT-1080 cells was observed by wound healing assay. The combined crude extracts showed a significant suppressive effects on MMP-2 and MMP-9 expression. To explore active inhibitory elements, the combined extracts were fractionated according to polarity into with n-hexane, 85% aqueous methanol, n-butanol, and water. Across these four solvent fractions, MMP-2 and MMP-9 activity and cell mobility in the HT-1080 cells were all strongly inhibited by the n-hexane fraction. These results suggest that C. soldanella extract and organic solvent fractions could be used as potent MMP inhibitors for effective anti-cancer treatments to suppress cancer invasion and metastasis.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.67-79
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• 2021

This study aimed to isolate wild yeasts from waters and soils on the riversides of Geumgang upstream in Geumsan, seashores of Cheongpodae in Taean and Suncheonman in Suncheon, Korea, and to investigate their unrecorded microbiological characteristics. Forty-nine species of wild yeasts, including Aureobasidium pullans YH 4-3, were isolated from 40 samples of Cheonnaegang in Geumgang upstream. Fifty-six species and 36 species of wild yeasts were also isolated from 80 samples of Cheongpodae Beach and Suncheonman Mashland, respectively. Species from Candida genus were isolated from all three locations. Among them, Candida michaelii NNIBRFG28278, Sporobolomyces japonicus NNIBRFG28271, Dioszegia buhagiarii NNIBRFG28279, Ustilago spermophora NNIBRFG28273, Nakazawaea pomicola NNIBRFG31590, Candida natalensis NNIBRFG31591, Candida pseudorhagii NNIBRFG31592, Candida santamariae NNIBRFG31593, Cutaneotrichosporon terricola NNIBRFG31594, and Meira nashicola NNIBRFG31595 represent newly recorded yeast strains in Korea. Almost all of these unrecorded yeasts were oval shaped, and Sporobolomyces japonicus NNIBRFG28271 and Candida natalensis NNIBRFG31591 have ascospores. All the strains grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract (YM) media, and Ustilago spermophora NNIBRFG28273 grew well in vitamin-free medium. Sporobolomyces japonicus NNIBRFG28271 grew well in 15% NaCl-containing YPD medium, and Ustilago spermophora NNIBRFG28273 and Dioszegia buhagiarii NNIBRFG28279 assimilated starch, and Ustilago spermophora NNIBRFG28273 produced ethanol.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.4
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• pp.459-468
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• 2022

Long-term data analyses and bioassay experiments were conducted to assess limiting nutrients in Cheonsu Bay and Taean sea. First, long-term nutrient data (2004-2016) provided by the National Water Quality Monitoring Network were used to assess potential limiting nutrients. Analysis of the long-term data showed that the dissolved inorganic nitrogen/dissolved inorganic phosphate (DIN/DIP) ratio was mostly below 16, with N limitation being dominant. A subsequent analysis using the concentration ratios of N, P, and Si showed that N limitation was dominant during summer and autumn but that Si limitation occasionally occurred during winter and spring in relatively limited areas. However, the dominant limiting nutrient was not determined. The nutrient analysis of the field water collected during the bioassay experiment showed that DIN/DIP revealed P limitation at all stations in March and May, whereas N limitation was dominant in July and October. In the analysis using the concentration ratios of N, P, and Si, P and Si limitation appeared in March and May, but there were points with no dominant limiting nutrient. However, N limitation was dominant in July and October. In the bioassay experiment for assessment of the actual limiting nutrient, the results showed no specific limiting nutrient in March, whereas NH₄⁺ and NO₃^- showed responses in May, July, and October, which confirmed that N was a substantial limiting nutrient directly involved in phytoplankton growth during this period.

• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.80-88
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• 2002

This study was carried out to investigate the native distribution and flowering characteristics of Lycoris genus which is endemic species in Asia. This study was summarized as fellows: Native distribution of Lycoris genus was situated in latitude 37- 24 degrees with high humidity of coastline. Mininum temperature of native area was at -10℃ during winter season. The leaf of L. squamigera, L. koreana, L. sangunea, L. sprengeri, L. incanata and L. flavescens emergenced in spring. The leaf of L. radiata, L. rdiata var pumila, L. aurea, L. traubii, L. albiflora and L. houdyshelli emergenced in autum. Bulb of Lycoris genus show a sympodial branching system which is composed of 14-23 scales and 2.8-5.2 leaves per each bulb at flowering time. The flower shape of L. squamigera, L. Koreana, L, aurea, L. incanata, L. sprengeri, L. sanguinea and L. flavescens was trumpet. The flower of L. radiata. L. radiata var pumila, L. albiflora, L. houdchelli and L. traubii. was spider.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.177-177
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• 2020

새만금 간척지는 1989년 '새만금간척사업'이 발표된 이래 1991년에 방조제 공사를 시작으로 2006년 물막기 공사를 완료하였고, 2009년 이후 방조제를 완공하여 현재는 40,100ha의 간척지가 조성되었다. 하지만 간척지는 장기적으로 환경에 악영향을 미치며 특히 간척으로 인한 해안경관의 가치 상실, 생태계 파괴 및 교란에 따른 변화가 크기 때문에 이에 대한 대책이 필요하다. 적극적인 식생의 도입이 그 대책의 하나가 될 수 있는데 이는 자연적으로 식물천이를 유도하고 동물들을 유인하여 생태적으로 건강한 환경을 조성하는데 기여할 수 있다. 따라서 본 연구에서는 새만금간척지의 대표적인 수목생육 제한 요인으로 판단되는 염분, 배수 및 통기성에 대한 시험연구를 진행하고자 '09~'11까지 초기에 김제광활에서 2ha의 시험포를 운영하였고, '12부터는 군산옥구에서 2ha의 시험포를 운영하고 있다. 수목을 심을 포지를 구획하기 전에 토양의 염분값을 낮추기 위해서 군산옥구의 경우, 2ha의 시험포를 포함한 전체 묘목장 부지(100ha) 중에서 일부구간(38ha)을 '09 부터 자연강우 담수제염을 통해서 제염을 진행하였다. 이를 통해 초기 염분 값이 18ds/m~20ds/m에서 8ds/m~10ds/m로 낮아졌다. 이렇게 수목이 자랄 수 있는 정도의 제염이 이루어진 이후에는 배수 및 통기성 부분에 초점을 맞추어 포지를 구획하였다. 단지1의 경우, 사전제염작업을 하지 않고, 5M간격의 암거를 설치하였고, 단지2의 경우, 사전강우제염을 진행하고, 10M간격의 암거를 설치하였다. 단지3의 경우, 사전강우제염을 진행하고 그 위에 0.4M의 준설토를 성토하여 포지를 조성하고 일부구간에 5M, 10M암거를 설치하였다. 2년 간의 수목 생존률 및 생장 모니터링을 통해서 각 단지에서의 수목생장의 적합성의 정도를 간접적으로 판단할 수 있었다. 단지3이 암거설치 간격 및 설치유무에 관계없이 수목이 자라는데 가장 적합했다. 다음으로 단지2, 단지1 순으로 나타났다. 염분 값을 낮추기 위한 자연강우 담수제염의 경우에는 문제가 되지 않지만 배수 및 통기성 개선을 위한 암거설치의 경우는 새만금 전체 식재구역에 적용하기에는 경제성이 떨어지므로 배수 및 통기성을 위한 별도의 방안이 요구된다. 또한 각 단지에서의 수목 생장모니터링을 지속적으로 시행하여 보다 장기적인 측면에서의 식재기반 조성기법을 고려할 필요가 있다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.27 no.1
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• pp.31-44
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• 2024

The coastal dune ecosystem is one of the ecosystems under the most development pressure in Korea. Therefore, it is necessary to study the ecological location and related ecological phenomena of coastal dune plants, but related studies are lacking. Through this study, we intend to conduct research on the structure and restoration of dune plants, focusing on the coastal dunes in Jeju Island, which are affected by artificial development pressure and the continuous increase in tourists among many coastal dunes in Korea. Ecosystems of coastal sand dunes for vegetation survey in Jeju Island are selected based on naturalness and preservation. In this study, 23 major coastal dunes on Jeju Island including Udo were selected. In the coastal dunes of Jeju Island, a whole species survey and quadrat survey were carried out. The vegetation survey at study sites were conducted on May to September 2022, when the vegetation is clearly visible. At the survey site, the dune area was identified at the beginning and the plant species were recorded until no more new species appeared. Vegetation survey in the field was performed by 103 quadrat establishments and was conducted using Braun-Blanquet method. A total of 277 species appeared, and the most common species were Vitex rotundifolia and Calystegia soldanella. The frequency of both Vitex rotundifolia and Calystegia soldanella was approximately over 90%. The proportion of woody and herbaceous in all emerging species was 7.2% and 92.8%, respectively. The total number of species found in the quadrat survey was 98. As a result of classifying plant communities based on species dominance in the quadrats, it was analyzed into 30 plant communities. The plant communities that appeared with a frequency of 2 or more on the main island of Jeju were Vitex rotundifolia, Imperata cylindrica var. koenigii, Ischaemum antephoroides, Wedelia prostrata, Elymus mollis, Calystegia soldanella, Artemisia scoparia, and Tetragonia tetragonoides. The DCCA(detrended canonical correspondence analysis) based on the vegetation and environment factor matrix showed that the height and covers of the dominant plant species explain significantly the variation and distribution of coastal sand dune species on Jeju island. Thus, we may propose a plan to restore the coastal dunes of Jeju island as helping colonization and establishment of mainly sand dune native perennials and trees, preserving native plant communities that are declining and preserving present tree strips of Pinus thunbergii, Litsea japonica, Pittosporum tobira and Vitex rotundifolia.

• Korean Journal of Environmental Biology
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• v.42 no.1
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• pp.127-134
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• 2024

The feathery branched green alga Bryopsis plumosa (Hudson) C. Agardh bloomed at Songji Beach, Haenam, Korea, in November 2022. A terrible stench was present on the beach, and the bottom was covered in a thick green mat of green algae. The alga was identified as B. plumosa, which is an opportunistic species currently distributed worldwide. Dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) were analyzed in the bloomed area and compared with those of other vicinities. The DIN and DIP concentrations were 1.067 and 0.461 mg L^-1, respectively, which were 6 to 19 times higher than the average concentrations on southwest coasts, including Haenam. The B. plumosa bloom at Songji Beach in November 2022 appears to have depended on DIN and DIP concentrations in seawater. In this study, we report on the mass occurrence of B. plumosa, which appeared for the first time in Korea. This occurrence was found to be closely related to the concentration of nutrients in seawater. Therefore, it is necessary to manage the concentration of nutrients on land flowing into coastal waters to control green algal blooms such as Bryopsis.