• Childhood Kidney Diseases
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• v.12 no.1
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• pp.70-77
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• 2008

Purpose: Urinary tract infections(UTIs) are not uncommon findings in febrile pediatric patients and approximately one third of patients with UTI may have renal scars. This research was intended to establish the relationship between duration of fever and renal scars. Methods: The medical records of 143 patients were reviewed retrospectively. Inclusion criteria were as follows: 1) fever as defined by an axillary temperature $\geq37.5^{\circ}C$, 2) accurate history of fever duration and the use of antibiotics 3) no previous history of UTI and 4) positive urine culture. We observed whether the longer fever duration could be associated with the development of initial renal defects and subsequent renal scars, increased C-reactive protein(CRP), leukocytosis and the presence of vesicoureteral reflux(VUR). Results: 1) Patients with longer fever duration after antibiotics showed more frequent initial renal defects(P=0.014). However, fever duration before antibiotic use was not associated with the development of initial renal defects(P=0.244). 2) Incidence of renal scar increased with fever duration before antibiotic use(P=0.006) and fever duration after antibiotic use(P=0.015). 3) CRP correlated with the fever duration after antibiotic use(r=0.287, P=0.003). 4) There was no relationships between fever duration and VUR(P>0.05). Conclusion: Our data suggest that fever duration before/after antibiotic use is significantly associated with the increased development of renal scars in pediatric UTI.

• Journal of Life Science
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• v.22 no.6
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• pp.788-793
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• 2012

High concentration of antibiotics has been used to treat the outbreak of edwardsiellosis caused by Edwardsiella tarda in aquaculture. However, not all of the bacteria have been killed with high concentrations of antibiotics treatment by the formation of persister cells with a dormant state. The main objective of this study was to kill persister cell using antibiotics with the addition of natural plant compounds. Antibiotics used in this study consist of 100 mg/ml florfenicol and 100 mg/ml amoxicillin. Ten natural plant compounds with persister cell inhibitor activity to E. coli were obtained from Protein Engineering and Systems Biology Lab. of Sungkyunkwan University. The persister cell inhibition activities of those natural plant compounds were evaluated in test tube. Concentrations of the antibiotics were in the ranges of 25~200 ${\mu}g/ml$. The persister cell formation was observed after 16 hours of culture. Persister cells were killed by antibiotics with natural plant compounds. Among ten natural plant compounds, Gynostemma pentaphyllum, Mallotus japonicus, and Orixa japonica showed persister cell formation inhibition activities. The optimal concentrations of G. pentaphyllum, M. japonicus, and O. japonica for the inhibitor of persister cell formation were 100 ${\mu}g/ml$, 100 ${\mu}g/ml$, and 200 ${\mu}g/ml$, respectively. In vivo study was carried out to evaluate the effect of the antibiotics with natural plant compounds using aquacultural fish, olive flounder, as test animals. G. pentaphyllum, M. japonicus, and O. japonica of 30 ${\mu}g/ml$, 10 ${\mu}g/ml$, and 10 ${\mu}g/ml$ with antibiotics reduced cumulative mortalities, showing the effectiveness of persister cell inhibition.

• Pediatric Infection and Vaccine
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• v.6 no.2
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• pp.203-209
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• 1999

The beneficial effects of antibiotics in acute diarrhea of children were analysed 10 assess their effectiveness since many physicians use them to treat acute diarrhea. Searching 124 charts of patients admitted from August 1996 to July 1997, they were divided into two groups, an antibiotic negative (N) group of 28 patients and a positive (P) group of 96 patients. The admission period was significantly shorter in N group than P group (P=0.0057). Duration of fever and diarrhea were also shorter in N group (P=0.0328 and 0.0184 respectively). Although it was not statistically significant. duration of vomit and frequency of diarrheal episodes per day were lower in N group (P=0.7091 and 0.061, respectively). Age, sex, duration of diarrhea before the admission and CRP collected on the day of admission were not statistically different between the two groups (P=0.5866, 0.093, 0.3502 and 0.2628 respectively).

• Journal of Applied Biological Chemistry
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• v.64 no.2
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• pp.177-184
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• 2021

While veterinary antibiotics are used only in a part of the dose administered, the rest are excreted as urine or feces. Residual antibiotics enter the adjacent agricultural environments by spraying manure-based composts on farmlands and lead to secondary pollution. Therefore, it is necessary to develop the technique for post management such as regulatory levels of antibiotics in the agricultural environments. This study was conducted to compare by different matrices the amount of residual antibiotics such as tetracyclines and sulfonamides, which are known to be frequently used in Korea and to practice risk assessment by different antibiotics in soils before and after application of composts. Pre-treatment with modified typical method using buffer and solid phase extraction showed the recovery of composts and soils was more than 70% at ppb level and the limits of detection were 0.13-0.46 and 0.05-0.25 ㎍/kg, respectively. Analysis of manure-based composts revealed concentrations from 5.38 to 196.0 ㎍/kg for tetracyclines, from below the detection of limit (BDL) to 259.0 ㎍/kg for sulfonamides. In case of agricultural soils, residual concentrations of selected veterinary antibiotics were ranged 0.30-53.3 ㎍/kg, BDL-4.16 ㎍/kg respectively and the concentration level of tetracyclines, which had higher soil distribution coefficient (Kd) values, was higher than that of sulfonamides. There was a difference in human risk assessment by different antibiotics in soil before and after application of composts. But, it was indicated that detection values of all of 5 antibiotics were very safe on the basis that Hazard Quotient was safe below 1.

• KOREAN POULTRY JOURNAL
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• v.42 no.5
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• pp.125-129
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• 2010

2012년부터 사료내 첨가하는 항생(균)제가 전면 금지될 예정으로, 정부의 친환경 정책이 더욱 확대되면서 항생제 대체제에 대한 관심이 그 어느 때 보다 높아지고 있다. 경기도 부천에 위치한 네오그린(대표 정헌주)은 이러한 문제를 해결하기 위해 다년간의 연구 결과 병원성 세균만을 골라 잡아먹는 미생물제를 개발하면서 미래의 항생제 대체제로 손색이 없다는 평가를 받아오고 있어 화제를 모으고 있다. 양돈업계에서는 이미 제품의 우수성을 인정받아 많은 농가에서 사용하고 있으며, 양계 분야에도 시험사육과 소문을 통해 그 가치를 인정받고 있다. 본고는 벤처 중소기업인 네오그린과 그 제품을 사용하고 있는 농가를 방문, 항생제 대체제의 성공 가능성을 진단해 보았다.

• 농업기술회보
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• v.51 no.3
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• pp.25-26
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• 2014

가축사료에 항생제 사용이 전면 금지되고 친환경 축산물에 대한 소비자들의 관심이 높아지면서 축산농가에서는 자연스럽게 합성항생제를 대체하는 물질이나 기술에 대한 관심이 높아지고 있다. 최근 미생물과 봉독기술을 활용한 항생제 저감 돼지고기 생산모델을 양돈농가에 접목하여 생산성 향상과 고품질 친환경축산물 생산으로 소득이 증대한 사례가 있어 양돈농가에 소개하여 농가의 경영에 도움이 되었으면 한다.

• Monthly Korean Chicken
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• v.16 no.4
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• pp.60-64
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• 2010

• Monthly Korean Chicken
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• v.16 no.4
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• pp.72-76
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• 2010

• 사료
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• s.8
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• pp.61-65
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• 2004

세계적인 웰빙바람의 영향으로 축산물 뿐만 아니라 모든 식품에 대한 안전성 확보가 강조되고 있다. 소비자들의 이해를 돕고자 우리 축산물에 대한 항생제 남용방지 노력을 소개한다.

• Monthly Korean Chicken
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• v.17 no.6
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• pp.55-57
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• 2011