• Korean Journal of Food Science and Technology
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• v.43 no.4
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• pp.464-468
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• 2011

Halocynthia roretzi is one of the most important cultured marine species on the southern coast of Korea. Samples were extracted using methanol (ME), ethanol (EE) and water (WE) to evaluate the antioxidant activities and antilipase activity in Halocynthia roretzi extracts. Antioxidant potentials of the samples were determined by poly-phenol content, flavonoid content, free radical scavenging activity, reducing potential, and chelating activity. The ME showed significant scavenging activity (1176 ${\mu}g/mL$ IC50 for DPPH, and 895 ${\mu}g/mL$ IC50 for ABTS assay). The IC50 for lipase inhibition activity was 12,021, 6,004, and 14,979 ${\mu}g/mL$ in the ME, EE, and WE, respectively. In conclusion, Halocynthia roretzi extracts exhibited antioxidant activities and anti-lipase activity. These results suggest that Halocynthia roretzi extracts can be potentially used as a source of antioxidant and antiobesity agents.

• Journal of the Korean Applied Science and Technology
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• v.33 no.4
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• pp.717-725
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• 2016

This study was designed to examine the in vitro antioxidant, antimicrobial and anti-inflammation effects of essential oils of Erigeron annuus L. Flower. Erigeron annuus L. essential oils were obtained by solvent extraction. Antioxidative ability was evaluated by bioassays using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging effect and 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity. Erigeron annuus L. essential oil exhibited free radical scavenging activity on ABTS and DPPH 98.6%, 48.3% respectively, at a concentration of $500{\mu}g/ml$. Antimicrobial activity of essential oils of Erigeron annuus L. were tested against Staphylococcus aureus (S. aureus), Propionibacterium acnes (P. acne) and Escherichia coli (E. coli) by paper disc method, MIC and MBC. Erigeron annuus L. essential oil showed excellent antibacterial activities against S. aureus with MIC and MBC values of 0.31 mg/mL. The clear zone, indicating antimicrobial activity against P. acnes, was 14 mm, MIC and MBC values 0.31 mg/mL, 0.63 mg/mL, respectively. For the anti-inflammatory activity in RAW 264.7 cell, the Erigeron annuus L. essential oils inhibited not only NO production but also the expression of pro-inflammatory cytokines such as, TNF-${\alpha}$, IL-6 in a dose-dependent manner. These results suggested that Erigeron annuus L. essential oils has considerable potential as a cosmetic ingredient with antioxidative, antimicrobial and anti-inflammation effects.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.3 s.58
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• pp.161-171
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• 2006

In this study, we investigated the whitening and anti-oxidant activity of 37 Jeju island native plants. The active ingredients of the plants were prepared by methanol extraction. Whitening activity of plant extracts was examined from the inhibitory effect of tyrosinase and the inhibition of melanin synthesis of the B16-F1 cell line. Their anti-oxidant activity was measured by electron donating ability of DPPH (1,1-diphenyl-2-picrylhydrazyl) and ROS (reactive oxygen species) scavenging activity in V79-4 lung fibroblast cells using DCF-DA (dichlorofluorescin diacetate). Cytotoxicity of the extracts on cell s based experiments was investigated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Also, the toxicity test using a rabbit and the human skin patch test were carried out for examining the safety of the extracts which showed the high whitening activity. It is interesting that the extracts of Lespedeza cuneata, Ligustrum lucidum (stem), Morus bombycis (stem) and Prunella vulgaris var. lilacina showed both potent whitening and anti-oxidant activities.

• Clean Technology
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• v.27 no.4
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• pp.306-314
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• 2021

Grape skins are a natural resource rich in antioxidants, but people only eat grape flesh and have discarded the skins. This study investigated the possibility of using grape skin extract as a raw material for functional cosmetics. The dried grape skin powder was put in distilled water and stirred for 1 h, and then the supernatant separated from the solid was used as an extract. The extract yield was 17.8 ~ 31.4%, and the total flavonoid and polyphenol contents in the extract were 1.8 ~ 2.5 mg-QE g-extract^-1 and 16.9 ~ 20.3 mg-GAE g-extract^-1, respectively. The extract effectively removed radicals of DPPH and ABTS, and the degree of scavenging increased with the concentration of the extract. The extract inhibited the collagen hydrolysis activity of collagenase, and the activity inhibition rate increased to 84.2% as the extract concentration increased. However, notable inhibition of tyrosinase by the extract was not found. As the extract of Chamaecyparis obtusa was added to the grape-skin extract, the tyrosinase inhibition rate increased, but the DPPH radical scavenging activity decreased. This study found that grape skin extract has a high antioxidant capacity and anti-wrinkle effect but a low whitening effect. However, by mixing the grape skin extract with the extract of C. obtusa in an optimal ratio, the whitening effect was improved with excellent antioxidant and anti-wrinkle effects.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.111-116
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• 2018

Roasting process of grains modifies their physicochemical characteristics that affect flavor, color, taste, and textures, as well as composition of bioactive compounds. We roasted oats at different temperatures (150, 200, and $250^{\circ}C$) and for different time periods (15 and 30 min). The polyphenol and flavonoid contents in different solvent extracts (methanol, fermented ethanol, and water) were also investigated. The total polyphenol and flavonoid contents were highest in the methanolic extract (135 mg gallic acid equivalent/g and 29 mg catechin equivalent/g, respectively, at $250^{\circ}C/30min$ roasting) and increased with roasting time and temperature. In addition, the avenanthramides were most abundant as accessed ($266{\mu}g/g$) in the methanolic extract upon roasting at $200^{\circ}C$ for 15 min. The radical scavenging activities, using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid scavenging, increased with roasting temperature and time. The roasting process may modify the physicochemical structure of oats, thereby, improving polyphenol extraction and antioxidant activity. The results of this study could be used for the manufacture of foods using roasted oats.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.1
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• pp.49-58
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• 2024

Elaeocarpus sylvestris var. ellipticus is an evergreen tree of the family Elaeocarpaceae, which is a plant that grows naturally only in subtropical regions due to its vulnerability to cold. E. sylvestris has been reported to have flavonoids, coumarins, and polyphenols, and it is reported that these components have antibacterial and antioxidant effects. In this study, a roasting technique was introduced to increase the physiological activity of E. sylvestris, and antioxidant, total phenol, and total flavonoid content were measured after confirming changes in the ingredients of roasted E. sylvestris extracts and fractions. We analyzed the extracts before and after roasting and found an increase in the content of four components (brevifolin, ellagic acid, quercetin, and kaempferol), with the roasted extracts showing better antioxidant activity. The optimal roasting condition was confirmed to have the best antioxidant effect when roasting at 200 ℃ for 30 min, and the total phenol and total flavonoid content were also confirmed to be the best. E. sylvestris extract produced under optimal roasting conditions has been confirmed to exhibit improved antioxidant effects, and it is believed that it can be used as a cosmetic and food material in the future.

• Journal of Acupuncture Research
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• v.28 no.6
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• pp.35-42
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• 2011

목적 : Elastin 섬유는 피부, 폐, 동맥, 정맥 등 조직에서 발견된다. Elastin 섬유의 손실은 대동맥, 동맥, 세동맥의 탄력 등과 연관되어 폐기종, 혈액순환 부전 및 폐동맥 고혈압을 악화시킬 수 있다. 본 연구는 당귀약침액의 elastase 효소활성에 대한 효과와 항산화 효과에 대하여 연구하고자 계획되었다. 방법 : 당귀약침액의 elastase 억제효과와 DPPH, 및 NO 소거능을 측정하여 항산화 효과를 측정하였다. 결과 : 당귀약침액에서 통계적으로 유의한 elastase 활성 억제효과를 관찰할 수 있었다. 또한 통계적으로 강한 DPPH free radical 소거 효과가 관찰되었다. 그리고 NO 소거효과를 관찰할 수 있었으며, 그 효능은 pH 6.0에서 가장 강한 효능을 보였다. 결론 : 본 연구 결과에 의하면, 당귀약침액에는 유효한 elastase 활성 억제효과와 강한 항산화 효과가 있어, 폐기종 및 폐동맥 고혈압 치료에 유효한 작용이 있을 것으로 사료된다.

• Journal of Applied Biological Chemistry
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• v.60 no.4
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• pp.307-311
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• 2017

This study has been performed to identify the phenolic contents and antioxidant properties of methanol and ethanol extracts of young Camellia leaves in Jeju. Total polyphenol and flavonoid contents were 74.9 and 46.1 mg gallic acid equivalent/100 g, and 30.6 and 21.4 mg RE/100 g in the methanol and ethanol extracts, respectively. Based on $IC_{50}$ values, both extracts were also found to possess potent scavenging capacities of 1,1-diphenyl-2-picrylhydrazyl, nitric oxide and superoxide anion radicals, and ferrous ion chelating and reducing power activities. These results showed that young Camellia leaves is a good antioxidant source and holds promise as a natural ingredient in functional food and pharmaceutical supplement.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.331-339
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• 2001

목적 : 본 연구는 식풍해경(熄風解痙), 소산풍열(疏散風熱) 효능이 있는 백강잠이 치매에 미치는 영향을 알아보기 위하여 실험을 행하였다. 방법 : 치매 유발물질인 $amyloid{\beta}(A{\beta})$ 25-35를 흰쥐의 뇌 astrocyte에 처리한 후 대표적인 항산화 효소인 catalase, superoxide dismutase,glutathione peroxidase 및 glutathione-S-transferase의 활성 변화와 NO 생성 변화를 관찰하였다. 결과 $A{\beta}$ 25-35 처리로 catalase와 superoxide dismutase 활성이 현저히 감소하였으나 백강잠을 처리한 경우는 이들 효소 활성이 크게 증가하였다. 그리고, $A{\beta}$ 25-35의 농도에 의존적으로 증가된 NO 생성은 백강잠의 농도에 의존적으로 유의성 있게 억제되는 것으로 나타났다. 결론 : 백강잠은 항산화계 효소의 활성화 및 $A{\beta}$처리와 같은 치매유발 물질의 독성에 대한 적응능력 향상을 통하여 astrocyte를 보호하는 효능을 가지는 것으로 사료되며, 아울러 노인성 치매 등 임상적 응용에 그 효과가 기대된다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.851-855
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• 2013

This study was carried out to elucidate the effect of garlic extracts on the antioxidative activities of three isoflavones. All isoflavone samples showed greater antioxidative activity than butylated hydroxyanisole (BHA). In EDA (electron donating ability) tests, reducing power, SOD-like activity, and hydroxyl radical scavenging activity, all isoflavone samples with garlic extracts added showed significantly greater antioxidative effects than BHA. In conclusion, isoflavones have a potent antioxidative activity and garlic extracts have a big synergistic effect on this antioxidative activity.