The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Bombycis corpus on Amyloid-induced Lipid Peroxidation Antioxidative Enzymes and NO Synthesis in Rat Astrocytes

흰쥐의 뇌 astrocyte에서 $amyloid-{\beta}25-35$로 유발된 지질의 과산화와 항산화 효소계 및 NO 생성에 미치는 백강잠의 효과

  • Kim, Hee-Joon (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.) ;
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.) ;
  • Yoon, Cheol-Ho (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.)
  • 김희준 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 윤철호 (동국대학교 한의과대학 내과학교실)
  • Published : 2001.09.30
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Abstract

목적 : 본 연구는 식풍해경(熄風解痙), 소산풍열(疏散風熱) 효능이 있는 백강잠이 치매에 미치는 영향을 알아보기 위하여 실험을 행하였다. 방법 : 치매 유발물질인 $amyloid{\beta}(A{\beta})$ 25-35를 흰쥐의 뇌 astrocyte에 처리한 후 대표적인 항산화 효소인 catalase, superoxide dismutase,glutathione peroxidase 및 glutathione-S-transferase의 활성 변화와 NO 생성 변화를 관찰하였다. 결과 $A{\beta}$ 25-35 처리로 catalase와 superoxide dismutase 활성이 현저히 감소하였으나 백강잠을 처리한 경우는 이들 효소 활성이 크게 증가하였다. 그리고, $A{\beta}$ 25-35의 농도에 의존적으로 증가된 NO 생성은 백강잠의 농도에 의존적으로 유의성 있게 억제되는 것으로 나타났다. 결론 : 백강잠은 항산화계 효소의 활성화 및 $A{\beta}$처리와 같은 치매유발 물질의 독성에 대한 적응능력 향상을 통하여 astrocyte를 보호하는 효능을 가지는 것으로 사료되며, 아울러 노인성 치매 등 임상적 응용에 그 효과가 기대된다.

Keywords

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