한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제33권4호
  • /
  • Pages.717-725
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  • 2016
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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개망초꽃 에센셜 오일의 항산화, 항균 및 항염 활성

Antioxidant, Antimicrobial and Anti-inflammatory Activities of Essential Oil from Erigeron annuus L. Flower

  • 이미란 (제주대학교 생명과학기술혁신센터) ;
  • 전아림 (제주대학교 생명과학기술혁신센터) ;
  • 강창희 (제주대학교 생명과학기술혁신센터) ;
  • 부희정 (제주대학교 생명과학기술혁신센터)
  • Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Jeon, Ah-Lim (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kang, Chang-Hee (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
  • 투고 : 2016.10.28
  • 심사 : 2016.12.23
  • 발행 : 2016.12.30
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초록

본 연구는 개망초꽃 에센셜 오일의 항산화, 항균 및 항염 효능에 관하여 조사한 것이다. 개망초꽃 에센셜 오일은 용매추출법으로 추출하였다. 개망초꽃 에센셜 오일의 항산화 효능은 ABTS 라디칼 소거능과 DPPH 라디칼 소거 효능 측정으로 확인하였다. ABTS 라디칼 소거 효과는 $500{\mu}g/ml$ 농도에서 98.6%, DPPH 라디칼 소거는 48.3%로 좋은 효능을 나타내었다. 항균 활성은 S. aureus와 P. acnes, E. coli 균에 대하여 paper disc법과 최소저해농도(MIC)와 최소사멸농도(MBC)값을 측정하여 확인하였다. S. aureus 균주에 대해서는 MIC 값과 MBC 값이 모두 0.31 mg/mL 농도에서 우수한 억제 효과를 나타내었다. P. acnes 균에 대해서는 disc법에서 14 mm의 clear zone 형성과 MIC, MBC에서 각각 0.31 mg/mL, 0.63 mg/mL로 좋은 억제 효과를 보였다. 항염 활성에 관련하여 NO 억제 측정에서도 농도 의존적으로 NO의 생성을 억제시킴을 확인하였고, pro-inflammatory cytokine인 TNF-${\alpha}$, IL-6 역시 농도 의존적으로 억제하였다. 이러한 결과로부터 개망초꽃 에센셜오일은 항산화, 항균 및 항염 효능을 갖는 화장품 원료로서 개발 가능성이 있음을 시사한다.

This study was designed to examine the in vitro antioxidant, antimicrobial and anti-inflammation effects of essential oils of Erigeron annuus L. Flower. Erigeron annuus L. essential oils were obtained by solvent extraction. Antioxidative ability was evaluated by bioassays using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging effect and 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity. Erigeron annuus L. essential oil exhibited free radical scavenging activity on ABTS and DPPH 98.6%, 48.3% respectively, at a concentration of $500{\mu}g/ml$. Antimicrobial activity of essential oils of Erigeron annuus L. were tested against Staphylococcus aureus (S. aureus), Propionibacterium acnes (P. acne) and Escherichia coli (E. coli) by paper disc method, MIC and MBC. Erigeron annuus L. essential oil showed excellent antibacterial activities against S. aureus with MIC and MBC values of 0.31 mg/mL. The clear zone, indicating antimicrobial activity against P. acnes, was 14 mm, MIC and MBC values 0.31 mg/mL, 0.63 mg/mL, respectively. For the anti-inflammatory activity in RAW 264.7 cell, the Erigeron annuus L. essential oils inhibited not only NO production but also the expression of pro-inflammatory cytokines such as, TNF-${\alpha}$, IL-6 in a dose-dependent manner. These results suggested that Erigeron annuus L. essential oils has considerable potential as a cosmetic ingredient with antioxidative, antimicrobial and anti-inflammation effects.

키워드

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피인용 문헌

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  2. 식용곤충별 단백가수분해물의 항산화 활성 비교 vol.51, pp.5, 2016, https://doi.org/10.9721/kjfst.2019.51.5.480