• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.3
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• pp.263-273
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• 2012

Recent studies have uncovered attractive properties of para-coumaric acid (PCA) as a potential skin hywhitening agent. The purpose of the current study was to examine its UVB-shielding effects. Effects of PCA on the viability of HaCaT cells exposed to UVB were assessed in vitro in comparison with other aromatic amino acid metabolites that have similar UV absorption spectra. For in vivo test, PCA cream (1.5 %) and cream base were topically applied to the dorsal skin of SKH-1 hairless mice and the inflammatory responses due to UVB exposure were monitored by changes in skin color (erythema) and thickness (edema). The cream application-UVB exposure regimen was repeated every other day for a total of 12 sessions. When HaCaT cells were irradiated with UVB, there was a dose-dependent decline in cell viability. The cell viability decline due to UVB exposure (10 mJ $cm^{-2}$) was significantly prevented by 100 ${\mu}M$ PCA, cinnamic acid, urocanic acid, or indole acrylic acid by 39, 27, 39, or 31 %, respectively. Topical application of PCA cream onto the dorsal skin of hairless mice (10 ${\mu}g\;cm^{-2}$) attenuated the changes of color parameters, $L^*$, $a^*$, $b^*$ values, and thickness of the UVB (150 mJ $cm^{-2}$)-exposed skin by 59, 50, 58, and 53 %, respectively. The current study, together with the previous studies that demonstrated the antimelanogenic effects of PCA, suggested that PCA may prevent not only dyspigmentation but also inflammatory reactions in the UVB-exposed skin.

• Journal of Life Science
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• v.21 no.5
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• pp.713-719
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• 2011

In this study, we evaluated whether Lunasin Enriched Soybean (LES) suppressed 1-chloro-2,4-dinitrochlorobenzene (DNCB) induced allergic contact dermatitis in vivo. In the group treated with DNCB alone, erythema and hemorrhage in the skin were observed, while the application of LES after DNCB treatment modulated erythema and hemorrhage similar to the untreated group. In histopathological analysis of the skin, DNCB-treated groups showed increased skin thickness compared to the untreated groups, while the treatment of LES dose-dependently modulated skin thickness. Also, hypertrophy and hyperkeratosis of the epidermis, intracellular edema and infiltration of inflammatory cells were observed in the group treated with DNCB alone, while treatment of LES inhibited these inflammatory changes. DNCB also induced a significant up-regulation of iNOS and COX-2 expression, as compared to the untreated groups. However, the treatment of LES significantly decreased the down-regulation of iNOS and COX-2 expression, as compared to DNCB-treated groups. Also, LES reduced the IgE level in DNCB-stimulated groups. And lunasin from LES translocated the nucleus of the cells in the skin of Sprague-Dawley rats. Taken together, it is thought that LES has the potential to be used as an alternative treatment for allergic contact dermatitis.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.115-124
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• 2017

The objective of this study was to investigate skin whitening effect of Resorcinol dipentyl ether [1,3-di(pentyloxyl)benzene] by in vitro experiments. Resorcinol dipentyl ether was prepared by alkylation of resorcinol with 1-bromopentane. The reaction products were confirmed by NMR, MS and other analytical equipments. In order to evaluate the skin safety of resorcinol dipentyl ether, the cytotoxicity of the cells constituting the skin (keratinocyte, melanocyte, fibroblast) was analyzed and similar cell viability was observed in all cell lines as compared with the control group. Inhibition of extracellular melanin synthesis effect of resorcinol dipentyl ether was approximately 65.75% at $20{\mu}g/mL$ and inhibition of intracellular melanin synthesis effect of resorcinol dipentyl ether was approximately 53.89% at $20{\mu}g/mL$. The real-time PCR and western blot analysis of mRNA expression and protein expression of tyrosinase, TRP-1, and TRP-2 related to melanogenesis revealed that melanin inhibitory effect of resorcinol dipentyl ether was inhibited from the transcription stage respectively. Finally, this study suggested applicability of Resorcinol dipentyl ether [1,3-di(pentyloxyl)benzene] as a whitening functional cosmetic new material.

• Korean Journal of Microbiology
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• v.54 no.1
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• pp.38-45
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• 2018

Acne is an inflammatory skin disease that occurs in puberty and young people. Propionibacterium acnes (P. acnes) is known to be a major cause of inflammation in acne. P. acnes proliferates within hair follicles blocked by overproduced sebum in the skin, and thereby activates monocytic cells to promote the secretion of pro-inflammatory cytokines. In this study, we investigated the possibility of Cnestis palala (Lour.) Merr. extract to diminish P. acnes-mediated inflammatory responses. We found that C. palala extract significantly attenuated P. acnes-induced pro-inflammatory cytokine expressions, such as $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, iNOS, and COX-2 in mouse macrophage RAW264.7 cells. Moreover, we observed that C. palala extract inhibited $NF-{\kappa}B$ transcriptional activation, which is the major transcription factor of inflammatory cytokine expression. Therefore, it is expected that C. palala extract has a potential as a therapeutic agent or supplement for the treatment P. acnes-induced inflammatory responses.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.1
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• pp.63-71
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• 2004

The human skin is constantly exposed to environmental irritants such as ultraviolet, smoke, chemicals. Free radicals and reactive oxygen species (ROS) caused by these environmental facts play critical roles in cellular damage. These irritants are in themselves damaging to the skin structure but they also participate the immensely complex inflammatory reaction. The purpose of this study was to investigate the skin cell protective effect of Juniperus chinensis xylem extract on the UV and SLS-induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. We found that Juniperus chinensis xylem extracts had potent radical scavenging effect by 98％ at 100 $\mu\textrm{g}$/mL. Fluorometric assays of the proteolytic activities of matrix metalloproteinase-l(MMP-1, collagenase) were performed using fluorescent collagen substrates. UV A induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97％ at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79％ at 25 $\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. In this test Juniperus chinensis decreased expression of interleukin 6 about 30％. Expression of prostaglandin E$_2$, (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay (EIA) using PGE$_2$ monoclonal antibody. At the concentrations of 5-50 $\mu\textrm{g}$/mL of the extracts, the production of PGE$_2$ by HaCaT keratinocytes (24 hours after 10 mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p〈0.05). The viability of cultured HaCaT keratinocytes was significantly reduced at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB irradiation, but the presence of these extracts improved cell viability comparing to control after UVB irradiation. We also investigated the protective effect of this extract in sodium lauryl sulfate (SLS)-induced irritant skin reactions from 24 hour exposure. Twice a day application of the extract for reducing local inflammation in human skin was done. Irritant reactions were assessed by various aspects of skin condition, that is, erythema (skin color reflectance) and transepidermal water loss (TEWL). After 5 days the extract was found to reduce SLS-induced skin erythema and improve barrier regeneration when compared to untreated symmetrical test site. In conclusion, our results suggest that Juniperus chinensis can be effectively used for the prevention of UV and SLS-induced adverse skin reactions such as radical production, inflammation and skin cell damage.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.196-196
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• 1996

Artemisia extract powder (DA-9601)의 일반약리 작용을 mice, rat 및 rabbit에서 검토하였다. DA-9601은 rota-rod test에 의한 운동 협조능, 초산 writhing 반응, tail-flick test 및 randall-sellito test를 이용한 진통작용, thiopental sodium의 수면작용, 장관 수송능, carrageenan의 염증, 현수에 의한 근이완작용, 정상체온, strychnine의 경련작용, 수정관, 자궁각 및 Evans blue의 피부혈관 투과성에 별다른 영향을 미치지 않았다. DA-9601은 토끼의 적출회장 운동을 억제하였고 혈압 강하작용과 이뇨작용을 나타내었다

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.187-188
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• 2000

최근 자외선 차단제의 사용이 증가하면서 자외선 차단제 도포로 인한 자극성 피부염, 접촉 피부염, 광알레르기성 또는 광독성 피부염 등의 보고가 증가하고 있어 피부에 자극이나 알레르기 반응, 독성을 나타내지 않는 적절한 천연소재 자외선 차단제의 개발에 대한 필요성이 증대되고 있다. 이에 본 연구에서는 새로운 자외선 차단제 개발을 위한 기초 자료를 확보하기 위하여, 국내산 해조류 중의 자외선 흡수 물질의 분포를 조사하였다. (중략)

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.04a
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• pp.49-51
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• 2000

오존은 온도가 높고 태양 빛이 강한 여름철에 휘발성 유기화합물(Volatile Organic Compounds)과 질소화합물(NOx)의 광 화학 반응으로 생성되는 이차적 공해물질(Secondary Air Pollutant)이다. 성층권에 존재하는 오존은 피부암을 유발시키는 강한 자외선을 차단하고 흡수하는 고마운 존재이지만, 급속한 산업발달로 다량의 공해물질유출로 인해 생성된 지표면상의 오존은 대단히 유독해서 높은 농도에서 장시간 호흡 시 호흡기관의 장애는 물론이고 치사까지 이르게 하는 것으로 알려져 있다. (중략)

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2001.11a
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• pp.333-334
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• 2001

대기 중 포름 알데하이드 연구의 중요한 이유는 인체에 대한 악영향을 끼치는 화합물이라는 점과 대기 중 거의 모든 유기화합물들의 광산화 반응 1차 생성물인 카르보닐 화합물 가운데 가장 풍부하게 존재하고 라디칼의 중요한 기원자로 작용하기 때문이다. 인체에 대한 악영향의 현상으로는 눈, 코, 목을 자극하고 기관지 천식, 피부 알레르기를 유발시키는 것으로 나타나고 최근에는 동물과 인간의 발암물질로도 확인되어지고 있다. (중략)

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2002.04a
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• pp.207-208
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• 2002

고무는 자동차 타이어, 고무밴드, 고무장갑, 주형틀 등 산업활동 및 생활용품 등에 많이 사용되고 있다. 고무제조 공장에서는 다양하고 복합한 화학물질을 사용하여 열, 압력, 촉매반응 등 다양한 공정을 통해 고무제품을 제조하고 있다. 고무제품 중 타이어를 생산하는 공장 내 작업환경은 먼지, 가스, 퓸과 nitrosamines 등과 같은 화학부산물에 의해 오염된다 따라서, 근로자들은 호흡기, 피부 등을 통해 고무생산 및 제조공정에서 방출되는 유해물질들에 노출되고 있는 실정이다(NIOSH, 1993). (중략)