Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of LES on Recovery Capability of DNCB-Induced Allergic Contact Dermatitis in Rat

대두 추출물 LES가 DNCB로 유도된 랫드의 알레르기성 접촉피부염에 미치는 영향

  • Kim, Jeong-Sook (Department of Biological Science, Andong National University) ;
  • Park, Young-Mi (Department of Biological Science, Andong National University) ;
  • Jeong, Jin-Boo (Department of Medicinal Plant Resource, Andong National University) ;
  • Lim, Jae-Hwan (Department of Biological Science, Andong National University) ;
  • Jeong, Hyung-Jin (Department of Medicinal Plant Resource, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University)
  • 김정숙 (안동대학교 자연과학대학 생명과학과) ;
  • 박영미 (안동대학교 자연과학대학 생명과학과) ;
  • 정진부 (안동대학교 자연과학대학 생약자원학과) ;
  • 임재환 (안동대학교 자연과학대학 생명과학과) ;
  • 정형진 (안동대학교 자연과학대학 생약자원학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Received : 2011.02.07
  • Accepted : 2011.04.08
  • Published : 2011.05.30
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Abstract

In this study, we evaluated whether Lunasin Enriched Soybean (LES) suppressed 1-chloro-2,4-dinitrochlorobenzene (DNCB) induced allergic contact dermatitis in vivo. In the group treated with DNCB alone, erythema and hemorrhage in the skin were observed, while the application of LES after DNCB treatment modulated erythema and hemorrhage similar to the untreated group. In histopathological analysis of the skin, DNCB-treated groups showed increased skin thickness compared to the untreated groups, while the treatment of LES dose-dependently modulated skin thickness. Also, hypertrophy and hyperkeratosis of the epidermis, intracellular edema and infiltration of inflammatory cells were observed in the group treated with DNCB alone, while treatment of LES inhibited these inflammatory changes. DNCB also induced a significant up-regulation of iNOS and COX-2 expression, as compared to the untreated groups. However, the treatment of LES significantly decreased the down-regulation of iNOS and COX-2 expression, as compared to DNCB-treated groups. Also, LES reduced the IgE level in DNCB-stimulated groups. And lunasin from LES translocated the nucleus of the cells in the skin of Sprague-Dawley rats. Taken together, it is thought that LES has the potential to be used as an alternative treatment for allergic contact dermatitis.

본 연구에서는 대두 추출물인 LES가 DNCB에 의해 인위적으로 접촉피부염을 유발시킨 랫드의 피부 회복능에 미치는 효과를 조사하였다. 알레르기 접촉피부염이 유발된 피부에 LES 17.6 mg/ml를 3-5회 도포하여 피부 표면을 조직학적 분석하여 본 결과 각질이 제거된 깨끗하고 매끄러운 상태로 되었으며, 비후화된 표피층의 두께도 점차 정상군과 같은 두께로 회복시키는 효과를 나타냈다. 또한 LES는 체내 지질함량을 낮춰 정상수준으로 회복시키는 지질함량의 개선에 효과적으로 작용하고 있으며, 혈장 내 IgE의 수준도 정상군 수준으로 감소시키고 있으며, LES에 의해 염증 반응과 관련된 iNOS와 COX-2의 활성도 감소되어 피부의 과민반응 해소에 관여하는 것으로 생각된다. LES에 포함된 주 성분 중 하나인 lunasin은 세포 침투와 함께 핵 내로 이동하는 것으로 확인되어 전체 유전자 또는 일부 유전자의 발현을 조절하는 데 관여할 것으로 추측된다. 이러한 결과로 보아 LES는 알레르기성 접촉피부염에 매우 효과적인 치료 효과를 나타내고 있기 때문에 기존의 알레르기성 접촉피부염 치료제를 대체할 수 있는 제제로서 응용이 기대된다.

Keywords

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