• 제목/요약/키워드: 피부미생물

검색결과 140건 처리시간 0.184초

Lactobacillus plantarum APsulloc 331261 Fermented Products as Potential Skin Microbial Modulation Cosmetic Ingredients (Lactobacillus plantarum APsulloc 331261 발효 용해물의 피부 미생물 조절 효과)

  • Kim, Hanbyul;Myoung, Kilsun;Lee, Hyun Gee;Choi, Eun-Jeong;Park, Taehun;An, Susun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • 제46권1호
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    • pp.23-29
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    • 2020
  • The skin is colonized by a large number of microorganisms with a stable composition of species. However, disease states of skin such as acne vulgaris, psoriasis, and atopic dermatitis have specific microbiome compositions that are different from those of healthy skin. The target modulation of the skin microbiome can be a potential treatment for these skin diseases. Quorum sensing (QS), a bacterial cell-cell communication system, can control the survival of bacteria and increase cell density. Also, QS affects the pathogenicity of bacteria such as biofilm formation and protease production. In this study, we confirmed anti-QS activity of Amorepacific patented ingredients, which are Lactobacillus ferment lysate (using Lactobacillus plantarum APsulloc 331261, KCCM 11179P) through bio-reporter bacterial strain Chromobacterium violaceum. The purple pigment production of C. violaceum controlled by QS was reduced 27.3% by adding 10 ㎍/mL of Lactobacillus ferment lysate (freeze dried). In addition, the Lactobacillus ferment lysate increased growth of Staphylococcus epidermidis 12% and decreased growth of Pseudomonas aeruginosa 38.5% and its biofilm formation 17.7% at a concentration of 10 ㎍/mL compared to the untreated control group. Moreover, S. epidermidis was co-cultured with the representative dermatological bacterium Staphylococcus aureus in the same genus, the growth of S. epidermidis was increased 134 % and the growth of S. aureus was decreased 13%. These results suggest that fermented lysate using Lactobacillus plantarum APsulloc 331261 may be useful as a cosmetic ingredient that can control the balance of skin microbiome.

Novel Sporichthyaceae Bacterium Strain K-07 Skin Barrier, Moisturizing and Anti-inflammatory Activity (신규 Sporichthyaceae Bacterium Strain K-07 배양액의 피부장벽, 보습 및 항염증 활성)

  • Lee, Dong-Geol;Kim, Minji;Kang, Seunghyun;Kim, Youn-Joon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • 제43권2호
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    • pp.137-147
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    • 2017
  • The human skin is an ecosystem that provides habitat to various microorganisms. These comprise the skin microbiome and provide numerous benefits in addition to maintaining a symbiotic relation with the host. Various metabolites generated by the skin microbiome exert beneficial effects such as strengthening the skin barrier, and anti-aging and anti-inflammatory functions. In this study, we isolated a novel bacterium, designated Sporichthyacae strain K-07, from the human skin. Analysis of 16S rRNA gene sequences showed that the newly found bacterium shares 93.4% homology with the genus Sporichthya, thus corroborating the discovery of a novel genus. We further analyzed the effect of the novel strain in vitro, by treating HaCaT cells with bacterial metabolite products. Treatment resulted in changes in the mRNA expression levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, aquaporin3, IL-6, TNF-${\alpha}$, TSLP, and TARC. Specifically, the levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, and aquaporin3 were higher in strain K-07 metabolite product-treated cells than in control cells. These results showed that metabolite products of the novel strain K-07 enhanced the skin barrier and exert anti-inflammatory effects. Therefore, these metabolite products could be potentially used for treatment of skin conditions.

Zymomonas mobilis에 의해 생성된 Fructan (Levan)의 특성 및 화장품 원료로의 개발

  • 이재섭;양은경;이정하;김철호;박수남;이종원;김기호
    • Journal of the Society of Cosmetic Scientists of Korea
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    • 제28권1호
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    • pp.186-201
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    • 2002
  • Fructan(Levan)은 식물체 및 미생물에서 발견되는 탄수화물로 이는 과당(fructose)이 $\beta$-2, 6 결합으로 연결되어 있는 polysaccaride 이다. 본 연구에서는 Fructan을 생성하는 미생물(Zymomonas mobilis)과 10% sucrose(기질), 1-2% 효모 추출물을 주성분으로 하는 배지를 사용하여 30-37$^{\circ}C$, pH 5.0-7.0에서 20-24시간 동안 배양한후 원심분리하여 균체를 제거하고 3배량의 알코올을 가하여 침전, 건조하여 얻은 Fructan의 화장품 원료로서의 가능성을 조사하였다. 보습효과에 있어서는 Hyaluronic acid와 유사하였으며, keratinocyte에 대한 세포증식 효과를 나타내었다. 또한 3-D culture에 의해 구축된 생인공 피부내에 0.05%의 sodium lauryl sulfate (SLS)를 사용하여 피부자극에 의한 초기 염증 반응을 유도한후 0.01mg/m1, 0.05mg/m1의 Fructan을 각각 처리하였을 때, SLS만을 처리한 생인공피부와 비교하여 세포증식효능을 보였고, SLS 자극물질로 유도된 전염증성 조절인자인 interleukin-l$\alpha$(IL-l$\alpha$)의 분비량을 조사 하였을때 0.01mg/ml, 0.05mg/ml의 Fructan을 처리한 생인공피부의 IL-l$\alpha$ 양이 Fructan을 처리하지 않은 것보다 상대적으로 감소하였다. 이러한 결과로 Fructan이 생인공 피부내 피부 세포의 증식효과를 나타낼 뿐만 아니라, 또한 피부자극물질에 의한 염증반응에 대해 자극완화효능이 있음을 알 수 있었다. 섬유아세포 및 동물을 이용한 안전성 시험에서도 독성이 없는 안전한 원료로 평가되었다.

Effects of Cosmetics and Their Preservatives on the Growth and Composition of Human Skin Microbiota (피부 미생물총의 생장과 구성에 대한 화장품과 그 방부제의 영향)

  • Jeong, Jin-Ju;Kim, Dong-Hyun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • 제41권2호
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    • pp.127-134
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    • 2015
  • We investigated the growth-inhibitory activities of cosmetics and their preservatives against pathogens and resident skin bacteria. Of the tested cosmetics, preservatives such as parabens, 1,2-hexanediol, phenoxyethanol-contained toner, emulsion, cream and baby cream exhibited potent antibacterial effects against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Parabens, 1,2-hexanediol and phenoxyethanol inhibited the growth of pathogens, as well as skin-resident bacteria such as Staphilococcus epidermidis, Shigella flexneri, Enterobacter aerogenes and so on. The application of a basic cream containing phenoxyethanol to human skin was shown to disturb the skin microbiota: at the phylum level, Proteobacteria increased and at species level, 4P004125_s increased and Propionibacterium humerusii decreased. Based on these findings, parabens, 1,2-hexanediol and phenoxyethanol have antimicrobial activity and cosmetics containing phenoxyethanol may disturb skin microbiota.

A Study on the antimicrobial of cotton fabric by cationic compound (면직물의 카티온 유기제에 의한 항균성에 관한 연구)

  • 권윤정;차민경
    • Proceedings of the Korean Fiber Society Conference
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    • 한국섬유공학회 2003년도 봄 학술발표회 논문집
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    • pp.417-418
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    • 2003
  • 섬유제품을 착용하면, 땀ㆍ피지ㆍ때 등 피부로부터 대사 노폐물이 섬유표면에 부착되고, 의복이 오염되어 비위생적이 된다. 오염물이 부착된 섬유제품에서는 악취가 발생함과 더불어, 보온성이나 통기성이 저하하기 때문에 착용감이 나빠진다. 피부에 상존하는 세균이나 외부로부터 부착된 미생물은 섬유에 부착된 인간의 노폐물이나 오염물질 등의 유기화합물을 소화시켜 휘발성 악취물질을 발생한다.$^{1)}$ 항균 방취 가공은 미생물의 부착과 번식을 억제하고 나아가서 불쾌감을 없애주는 기술이다. (중략)

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Investigation of Antimicrobial Activity of Rutaceae Fruit Ethanol Extracts Against Microorganisms-induced Skin Inflammation

  • Kim, Mee-Kyung
    • Journal of the Korea Society of Computer and Information
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    • 제26권12호
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    • pp.237-245
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    • 2021
  • This study investigated the Antimicrobial activity of Rutaceae fruit ethanol extracts against microorganisms-induced skin inflammation in cosmetic materials. Rutaceae fruits were separated in two parts of whole fruit (pulp, pulp fegment membrane, peel) and peel, and extracted with 70% ehtanol. The results demonstrated that Rutaceae fruit ethanol extracts showed antimicrobial activity in 5 strains except Staphylococcus aureus. In particular, the antimicrobial activity against Staphylococcus epidermidis was the best in fresh lemons whole fruit. The antimicrobial activity against Escherichia coli was shown only in fresh lemon peel and fresh trifoliate peel. Additionally, antimicrobial activity against Propionibacterium acnes was shown only in the dried lemon peel. In the results of antimicrobial activity against Pityrosporum ovale, in the case of fresh fruits, citron whole fruits showed the highest effect, followed by lemon whole fruits and mandarin orange peel. And in the case of dried fruits, orange peel showed the highest effect, followed by trifoliate peel, mandarin orange peel and lemon peel. Therefore, it is considered that lemon, which shows antimicrobial activity against all skin inflammation-causing microorganisms, can be used as a natural material for improving skin inflammation in cosmetics.

Structure and Function of Tight Junctions in the Skin (피부에서의 치밀이음의 구조와 기능)

  • Song, Mee;Baek, Ji Hwoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • 제48권2호
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    • pp.181-188
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    • 2022
  • The skin protects the body from excessive water loss and the invasion of harmful substances, such as chemicals and microbes. The stratum corneum, is recognized as a very important physical barrier. However, in recent years evidence emerged that tight junctions (TJ) might also play a crucial role in barrier function of the skin. In the present study, TJ proteins including transmembrane proteins and plaque proteins, skin permeability barrier function and skin diseases of TJ were reviewed.

Antimicrobial activity of fraction mixture of ethanol extracts from Eucalyptus globulus, Yucca recurvifolia, and Melaleuca alternifolia against several human skin microbes (유칼립투스, 유카와 차나무의 추출분획 혼합물의 여러 인간 피부 상재균에 대한 항균활성)

  • Lee, Da-Sol;Hong, In Kee;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • 제55권1호
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    • pp.46-51
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    • 2019
  • This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.

Analysis of Research Trends in the Skin Microbiome (피부 마이크로바이옴의 연구 동향 분석)

  • Hyo-Jeong Son;Seon-Hee You
    • Journal of the Korean Applied Science and Technology
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    • 제41권3호
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    • pp.648-656
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    • 2024
  • On the skin, the microbiome, which includes bacteria, viruses, and fungi, coexist with human cells. Microbiome cosmetics are being developed actively in the field of skin and cosmetics recently. The skin microbiome is very diverse, and although the composition ratio and habitat are different, it nourishes the skin or restricts the proliferation of pathogens, which are competitors, through interactions in various ways. However, if the balance is broken, it can contribute to the destruction of immune homeostasis and lead to the development of skin diseases. Accordingly, this paper can be used as important data for the skin microbiome market, which has great future growth potential, by understanding the role and research trends of the skin microbiome, which is a symbiotic relationship with our skin, and by examining industrial trends in biological materials that help the microbiome balance.

Oxidant Activities in Human Dermal Microvascular Endothelial Cells Infected with Orientia tsutsugamushi (Orientia tsutsugamushi에 감염된 인간 피부 미세혈관 내피세포의 산화 활성)

  • Koh Young-Sang
    • Korean Journal of Microbiology
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    • 제41권3호
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    • pp.232-235
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    • 2005
  • Transcripts levels of superoxide dismutases increase slightly following infection of human dermal microvascular endothelial cells(HMEC-1) by the obligae intracellular bacterium Orientia tsutsugamushi, the causative agent of scrub typhus. In addition, fluorescence-activated cell sorter analysis demonstrates significant intracellular peroxide activity in infected cells within 5 hr after exposure to O. tsutsugamushi. Furthermore, infected cells experienced a significant depletion of glutathiones. These results support hypothesis that cells infected by this intracellular bacterium experience oxidant-mediated injury.