Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Antimicrobial activity of fraction mixture of ethanol extracts from Eucalyptus globulus, Yucca recurvifolia, and Melaleuca alternifolia against several human skin microbes

유칼립투스, 유카와 차나무의 추출분획 혼합물의 여러 인간 피부 상재균에 대한 항균활성

  • Lee, Da-Sol (Department of Biological Sciences, Kangwon National University) ;
  • Hong, In Kee (R&D Center, Radiant Ltd.) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • Received : 2019.01.25
  • Accepted : 2019.02.20
  • Published : 2019.03.31
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Abstract

This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.

이 연구에서는 유칼립투스, 유카와 차나무의 ethanol 추출물의 수지를 이용한 분획 혼합물에 대한 항균활성을 평가하였는데 여러 인간 피부 상재균에 낮은 최소저해농도(0.24~3.32 mg/ml)를 나타내었고 항생제 triclosan과 ampicillin보다 우수한 활성을 나타내었다. Time kill assay에서 식물 추출분획 혼합물은 4시간 이내에 미생물 균주의 개체수를 92% 이상 감소시켰고, 모든 미생물 균주의 뉴클레오티드를 상당량 유출시켰으며, 항균 효과는 이가 양이온($Mg^{2+}$과 $Ca^{2+}$)에 영향을 받지 않았다. 이러한 결과는 Eucalyptus sp., Yucca sp., 및 tea tree의 ethanol 추출물의 수지분획 혼합물이 중요한 인간 피부 상재균을 억제하는 효율적인 화장품 방부제로 이용될 수 있음을 시사한다.

Keywords

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Fig. 1. Growth of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; X, plant fraction mixture; △, triclosan or ampicillin).

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Fig. 2. Nucleotide leakage of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; △, plant fraction; X, triclosan or ampicillin).

Table 1. Minimum inhibitory concentration of antibiotics and plant fraction mixture against several human skin microbes

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Table 2. Effect of divalent cation on the antimicrobial activity of plant fraction mixture

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