Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Lactobacillus plantarum APsulloc 331261 Fermented Products as Potential Skin Microbial Modulation Cosmetic Ingredients

Lactobacillus plantarum APsulloc 331261 발효 용해물의 피부 미생물 조절 효과

  • 김한별 ((주)아모레퍼시픽 기술연구원) ;
  • 명길선 ((주)아모레퍼시픽 기술연구원) ;
  • 이현기 ((주)아모레퍼시픽 기술연구원) ;
  • 최은정 ((주)아모레퍼시픽 기술연구원) ;
  • 박태훈 ((주)아모레퍼시픽 기술연구원) ;
  • 안수선 ((주)아모레퍼시픽 기술연구원)
  • Received : 2020.01.31
  • Accepted : 2020.03.12
  • Published : 2020.03.30
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Abstract

The skin is colonized by a large number of microorganisms with a stable composition of species. However, disease states of skin such as acne vulgaris, psoriasis, and atopic dermatitis have specific microbiome compositions that are different from those of healthy skin. The target modulation of the skin microbiome can be a potential treatment for these skin diseases. Quorum sensing (QS), a bacterial cell-cell communication system, can control the survival of bacteria and increase cell density. Also, QS affects the pathogenicity of bacteria such as biofilm formation and protease production. In this study, we confirmed anti-QS activity of Amorepacific patented ingredients, which are Lactobacillus ferment lysate (using Lactobacillus plantarum APsulloc 331261, KCCM 11179P) through bio-reporter bacterial strain Chromobacterium violaceum. The purple pigment production of C. violaceum controlled by QS was reduced 27.3% by adding 10 ㎍/mL of Lactobacillus ferment lysate (freeze dried). In addition, the Lactobacillus ferment lysate increased growth of Staphylococcus epidermidis 12% and decreased growth of Pseudomonas aeruginosa 38.5% and its biofilm formation 17.7% at a concentration of 10 ㎍/mL compared to the untreated control group. Moreover, S. epidermidis was co-cultured with the representative dermatological bacterium Staphylococcus aureus in the same genus, the growth of S. epidermidis was increased 134 % and the growth of S. aureus was decreased 13%. These results suggest that fermented lysate using Lactobacillus plantarum APsulloc 331261 may be useful as a cosmetic ingredient that can control the balance of skin microbiome.

피부에는 여러 종의 미생물들이 군집을 이루며 서식하고 있고, 서로 상호작용을 하며 균형을 유지하고 있다. 하지만, 여드름, 건선 및 아토피 피부염과 같은 질병 상태에서는 피부 미생물의 균형이 깨져 건강한 피부와는 다른 미생물 군집 구성을 보인다. 피부 미생물 군집 구성의 조절은 이러한 피부 질환에 대한 잠재적인 치료 방법이 될 수 있다. Quorum sensing (QS)은 세포간 전달인자로, QS을 조절하면 유해균의 바이오필름 형성이나 효소 분비등을 억제할 수 있고, 이 역시 피부 미생물 군집 구성에 영향을 준다. 이번 연구에서는 아모레퍼시픽의 특허 성분인 유산균 발효 용해물(Lactobacillus plantarum APsulloc 331261, KCCM 11179P 이용)의 QS억제능력 및 피부 유익균과 피부 유해균의 생장에 미치는 영향을 확인하였다. 유산균 발효 용해물 10 ㎍/mL 처리시, QS에 의해 제어되는 Chromobacterium violaceum의 자주색 안료 생산을 대조군 대비 27.4% 감소시켰다. 또 피부 유익균인 Staphylococcus epidermidis의 생장을 대조군 대비 12% 증가 시켰고, 피부 유해균인 Pseudomonas aeruginosa의 생장을 38.5% 감소, 바이오필름 형성을 17.7% 감소시켰다. S. epidermidis를 같은 속(genus)에 포함되어 있는 대표적인 피부 유해균 Staphylococcus aureus와 함께 공배양 하였을 때 S. epidermidis의 생장은 대조군 대비 134% 증가시켰고, S. aureus의 생장은 13% 억제시켰다. 이러한 결과들을 종합하였을 때 L. plantarum APsulloc 331261을 이용한 발효 용해물은 피부 미생물의 균형을 조절해 줄 수 있는 화장품 원료로 유용하게 사용 될 수 있다고 사료된다.

Keywords

References

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