• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.89-90
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• 2001

식품에서의 갈변현상은, 외관을 손상시킴으로 인해 상품가치를 저하시킬 뿐만 아니라 경우에 따라서는 독성물질을 생성하는 등의 문제를 일으킨다. 대표적인 것으로 사과ㆍ바나나의 껍질을 벗긴 후에 진행되는 갈색화 또는 수산물에서의 새우ㆍ가재류의 흑변 현상이 잘 알려져 있다. 이러한 현상은, polyphenoloxidase(PPO, EC 1.14.18.1)에 의해 유리 tyrosine등의 phenol성 화합물이 quinone체로 산화하고, 생성 된 quinone 또는 유도체들은 중합되어 melanine색소를 형성하는 것이 원인으로 알려져 있다. 현재, 화학적 합성품인 4-hexylresorcinol의 침가가 흑변 현상의 방지책으로서 인정되어 있으나, 최근 소비자들의 사고를 고려할 때, 안전성이 높은 천연물 유래의 유효한 첨가물의 개발이 요망되고 있다. (중략)

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.131-135
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• 1998

Cathamus tinctorius, Coptis japonica and Asarum sieboldii were tested as substrate for the production of Flammulina velutipes. Among the C. tinctoris , C. japonica and A. sieboldii , C. tinctoris was the best substrate for the production of fruitbody. The effects of addition of C. tinctoris to sawdust substrate resulted in the increased mycelial growth on inoculum culture, 3.1% in ratio of fully culture and shorted one day in culture period. C. tinctoris was decreased 6.1% in ratio of fully culture, 11.0% in ratio of fruitbody productive culm. The addition of C.tinctoris, C.japonica to sawdust substrate increased 134.6%, 114.1% on the yield of the mushroom fruitbody respectively . But A. sieboldii decreased the mycelial growth and pineheading ratio delayed the production of fruitbody.

• Journal of Mushroom
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• v.8 no.3
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• pp.109-115
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• 2010

We investigated the morphological traits, hardness and color of fruting bodies on eight strains according to changes of the humidities in mushroom house in Flammulina velutipes. The morphological traits like length and thickness of stipe, diameter and thickness of pilei didn't show any relations to humidity. Variation of these traits were rather showed by strains. ASI 4065 and ASI 4031 produced the longest stipes and ASI 4103 did the shortest ones. Fruiting body of ASI 4103 showed the most thickness of stipe, diameter and thickness of pilei. The pilei of ASI 4065, ASI 4021 and the stipe of ASI 4153 was the thinest. Brightness (L) values of white strains were increased at the low humidity except ASI 4021. But chroma (a, b) value showed little variation according to the humidity. L value of brown strains usually increased at the low humidity and a chroma (a, b) value showed little variation according to the humidity. But the chroma (a) value of pilei increased and the chroma (b) value of pilei decreased at the hight humidity. Most strains were brighter at pilei than at stipes. Hardness were not any relations to the humidity. In general, hardness of stipes were higher than that of pilei and their degrees were different according to strains. Hardness of ASI 4021 was the highest and ASI 4166 was the lowest. White strains were higher than brown strains at the hardness. As a result, hardness was rather affected by strains than by humidity.

• The Korean Journal of Mycology
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• v.4 no.1
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• pp.31-44
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• 1976

The studies were carried out to examine the effects of supplementation of nutritional substances and physical conditions in substrate on the mycelial growth and yield of fresh sporophores of winter mushroom, Flammulina velutipes(Curt. ex Fr.) Sing. and to obtain further informations on the nutritional requirements of the fungus with reference to improvement of substrate through [analysis of chemical composition of the substrates during the cultivation period. The results obtained are summarized as follows: 1. The best yield of fresh sporophores, 84.4 g per 280 g substrate in a bottle, was obtained from the mixture of poplar sawdust 10 and rice bran 3 by volume when Flammulina velutipes was cultivated on the poplar sawdust supplemented by rice bran, wheat bran, cattle manure and various combinations of these materials as nutrient sources. The substrates of poplar sawdust 10 plus rice bran 3 and 2 or wheat bran 3 with a higher yield of fresh sporophores showed a comparatively higher content of total nitrogen. total sugar, and potassium. 2. The mycelial growth of the fungus was compared on the substrates of poplar sawdust supplemented by the several nutrient sources and poplar sawdust alone. The fastest linear growth occurred on substrates of poplar sawdust alone and poplar sawdust plus cattle manure deficient in sugar and nitrogen sources, but mycelial density was more sparse on the substrates. Also, growth in a solution extracted from these substrates was very meager. 3. In the substrates which varied with bulk density and moisture content optimum bulk density and moisture content for mycelial growth was 0.2g/cc and 72% on a dry weight basis, respectively, but the highest yield of fresh sporophores was obtained at the bulk density of 0.3g/cc and moisture content of 67%. 4. By increasing the ratio of rice bran in poplar sawdust the loss of total weight and ash, content at each stage was increased, and during the cultivation period of 75 days, loss of total weight of the substrates at inoculation was 17.8 to 28.8% and ash content increased about 12%. 5. 11 to 14% of the cellulose and 3 to 4% of the lignin content per original substrate were decreased without a great difference depending of the mixing ratio of rice bran. The soluble glucose concentration in the substrates was increased during the same period. 6. In the process of vegetative and reproductive growth of the fungus upon the substrates, the total nitrogen was increased in quantity per dry weight of sample but was reduced in absolute quantity to a minute extent. There is no great changes in content of organic nitrogen including amino acid nitrogen, and hydrolysable ammonium nitrogen during the vegetative growth period, but occurrence of sporophores resulted in a decrease in the nitrogen content of these forms. On the one hand, by an increase of additive amounts of rice bran, nitrogen contents of these forms were higher and the reduction range during the reproductive growth period became wider. 7. Mycelial growth of the fungus was accelerated in various liquid media supplemented with organic nitrogen sources such as peptone and yeast extract in comparison with addition of inorganic nitrogen sources. Furthermore, mycelial growth was mere vigorous in the media with higher content of organic nitrogen sources.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.9
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• pp.1335-1339
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• 2010

A total of 550 samples were analysed for nitrate contents using HPLC-UV. Nitrate contents of leaf vegetables were higher than those of root vegetables and fruit vegetables. The average nitrate content of the radish leaves (4875.8 mg/kg) was the highest, followed by marsh mallow (4711.7 mg/kg), crown daisy (4546.9 mg/kg) and vitamins (4239.5 mg/kg). The nitrate content in fruits of strawberry and banana averaged at 24.0 mg/kg and 438.5 mg/kg, respectively. Nitrate was not detected in other fruits. In fruiting vegetables nitrate contents were less than 1000 mg/kg. In onion, lotus root and radish, nitrate contents were 253.7mg/kg, 352.4mg/kg and 2849.0 mg/kg, respectively, with no detection in garlic. Nitrate contents in mushrooms were less than 100 mg/kg.

• Journal of Mushroom
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• v.3 no.1
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• pp.35-39
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• 2005

Highly Activated Calcium(below HAC) is the oxidized calcium made by dissolving shell materials with high voltage about 15,000V and high temperature ($1,500{\sim}5,000^{\circ}C$). This HAC is a material with a very high degree of purity without toxicant. This HAC decreases chemical reaction so the degree of being active and dissolving living material is outstanding. The effects of HAC on the propagation of mushrooms are following. In the case of the Pleurotus ostreatus, when controlling media moisture by mixing with the HAC, mycelium cultivating days were shortened by 2days. The day required for primordial formation after inoculation(DPI) were one day faster. The number of stem was 15 and individual weight was 248g/850cc, a 6.5% increase. In the case of Pleurotus eryngii, when controlling media moisture by mixing with the highly activated calcium, mycelium cultivating days were shortened by 3days. DPI were l day faster. The day required for colonization after inoculation was shortened by 6days and individual weight was 108.8g/850cc, a 9.7% increase. In the case of Flammulina velutipes, the highly activated calcium was the best for scraping up mycelium. Mycelium incubating days were shortened by 2days. DPI were shortened by 3days. The day required for colonization after inoculation was 1day faster and the period of cultivation was shortened by 3days. Individual weight was 165g/850cc, a 6.7% increase. In the case of Lentinula edodes, when mixing media with the highly cultivated calcium, cultivating days were shortened by 3 days. The days for becoming brown in color were 2 days faster and the days of the first harvest were shortened by 4 days. The weight of mushrooms was 169g/2kg, a 9.7% increase.

• Journal of Animal Environmental Science
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• v.18 no.sup
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• pp.35-40
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• 2012

This study was to performed to investigate the effect of horseradish powder and mushroom waste in fattening pig diet on odorous compound concentration from slurry. Twenty fattening boars [Landrace${\times}$Yorkshire) ${\times}$ Duroc] weighing an average body weight of $78.4{\pm}8.88$ kg were randomly assigned to one of 4 treatments (control, horseradish 0.03%, mushroom waste 0.5%, and combination; horseradish 0.03% + mushroom waste 0.5%). This experiment was a randomized complete block (RCB) design using 5 pigs per treatment with 1 pig per metabolizable cage. Pigs were fed experimental diet (amount proportional to 3% of their body weight) twice a day (09:00 and 16:00) for 7 d after having 14 d adaptation period. Experimental diets were mixed with water by 1:2.5 v/v. Odorous compounds in slurry including acetate, propionate, butyrate, trans fatty acid isomers were not different (P>0.05) among treatments. There was no difference (P>0.05) in the levels of phenol compounds including phenol and p-cresol in slurry among treatments. Concentration of indole was lower (P<0.05) in horseradish group compared with others. Skatole level was highest in control and horseradish group, middle in mushroom waste group, and lowest in combination group (P<0.05). Concentration of indole compounds was lowest (P<0.05) in combination group. Data from our current study suggest that indole compounds in slurry can be reduced by administrating pigs with 0.03% horseradish powder and 0.5% mushroom waste in their diet.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.70-78
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• 2001

The objective of this study was to determine effects of corn germ meal(CGM) and condensed molasses fermentation solubles(CMS) as the replacement of rice bran on mycelial growth and density in various mushrooms. The results of this study showed that CGM and CMS might be utilized more efficiently than rice bran in culturing mushrooms. The results are as follows; 1. Mycelial growth of Pleurotus ostreatus was remarkably higher than that of control when the concentration of CGM and CMS was 10%, respectively. However, the mycelial density was lower than that of control. Thus, Considering the mycelial growth as well as density, it was found that 20% supplements of CGM and CMS resulted in the best with 78mm/10d and 71mm/10d, respectively. 2. Mycelial growth of Pleurotus eryngii in the treatment group of 10% CMS and 20% CGM showed 74mm/10d and 67mm/10d, respectively. This result tended to be equal or somewhat lower compared with that of control(74mm/10d). 3. Mycelial growth of Flamulina velutipes was 87mm/10d in the treatment of 10% CMS, 79mm/10d in the treatment of 20% CGM, which showed significantly higher than that of control(56mm/10d). When the contents of CGM and CMS were mixed with the amount of 10-30%, Thus, it was assumed that the quantity of mushrooms may increase with the supplements of CEM and CMS. 4. Mycelial growth of Lentinus edodes in the treatment group of 10-40% CMS and 10-30% CGM showed 58-52mm/10d and 56-49mm/10d, respectively. This result was significantly higher that control(47mm/10d). 5. When CMS and CGM were supplememted with the level of 10-30%, mycelial growth rate of Ganoderma lucidum showed 76-72mm/10d and 74-69mm/10d, respectively, which are higher than those of control.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.626-632
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• 2001

The plant extracted from Nameko, Gallic, Green tea, Allspice, Polygonum multiflorum, Schizandra chinensis, Armeniacae and Pine needle were utilized to investigate the effects of extracts on free radical reaction, lipid oxidation and nitrite scavenging ability. The pH of ethanol extracts showed a higher than that of hot water extracts, among of which were showed the lowest pH 3.0 in Schizandra chinensis. The important factor of lipid oxidation were $Fe^{2+}$ ion and active oxygen, in which were bound by plant extracts in case of $Fe^{2+}$ ion existed. However, the hydroxyl radical scavenging ability of extracts were lowed, compared to extracts reacted with $Fe^{2+}$ ion. Among of them, the hydroxyl radical scavenging ability of Nameko and Pine needle extracts had a lower TBARS value than those of control. The iron content of extracts were less than 2.0 mg/100 g, but the total iron content of Schizandra chinensis extracts were 6.8 mg/100 g. The ethanol extracts of pine needle were higher than those of hot water extracts on the basis of $Fe^{2+}$ ion content. The ascorbic acid content of green tea showed 14.3 mg/100 g in hot water extracts and 16.7 mg/100 g in ethanol extracts. Electron donating ability of extracts showed more than 50%, except Nameko and allspices, which were higher in ethanol extracts than those of hot water extracts. The superoxide dismutase(SOD)-like activity of green tea showed 85.3% and 63.5% in hot water and ethanol extracts, respectively. The nitrite scavenging ability of green tea was the most effective in both extracts.

• Journal of Food Hygiene and Safety
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• v.39 no.1
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• pp.9-15
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• 2024

This study was performed to survey the distribution of Listeria spp. in fresh agricultural products in the Busan area, the Republic of Korea, from January to November 2022. We investigated the pathogenicity and epidemiological relationships by tracing isolated strains using polymerase chain reaction and pulsed-field gel electro-phoresis (PFGE) methods. Forty cases of Listeria spp. were detected in the 210 samples of fresh agricultural products analyzed. Four species, Listeria rocourtiae, L. innocua, L. grayi, and L. monocytogenes were detected only in green vegetables (lettuce, perilla leaps) and the others (L. innocua, L. monocytogenes, and L. grayi) were detected in enoki and oyster mushrooms. L. innocua was detected in 22 samples and L. grayi in six samples. L. monocytogenes, which causes foodborne diseases, was only detected in enoki mushrooms and the strains that were isolated had genes responsible for the pathogenicity of listeriosis (iap, prfA, inlA, inlC, inlJ, and hly). To investigate the genetic similarity and contamination route of L. monocytogenes, serotyping and PFGE were conducted for 12 strains isolated from fresh agricultural (10 strains) and poultry (2 strains) products distributed at a market in the Busan area. Two serotypes (1/2a, 1/2b) were detected in strains isolated from the agricultural and poultry products, but serotype 1/2b was only detected in strains isolated from agricultural products. PFGE analysis showed index of similarity values of 45.7 to 100% and the same patterns were represented in isolates from some enoki mushrooms. These isolates had the same serotypes and showed significant epidemiological relationships.