• Title/Summary/Keyword: 클론

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Dynamic Clonal Selection Algorithm with Gene Library Evolution using a Hypermutation (초돌연변이(Hypermutation)를 이용한 유전자 라이브러리 진화와 동적 선택 알고리즘)

  • 김정원;최종욱;김상진
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 2002.05a
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    • pp.417-422
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    • 2002
  • 인공면역시스템을 이용한 침입탐지시스템 개발을 위해 적용한 동적 클론 선택(Dynamic Clonal Selection) 알고리즘과 그의 문제점을 소개하고 보다 개선된 동적 클론 선택 알고리즘을 제안한다. 이전 연구에서 침입탐지시스템이 흔히 접하게 되는 상황, 즉 과거 안정적으로 관찰되었던 정상행위가 합법적인 요인들로 인하여 갑작스러운 변화를 보일 경우 과거 생성되었던 기억탐지자가 정상행위를 비정상행위로 오류 판단하는 것을 막기 위하여 인간면역시스템의 체세포 돌연변이 (somatic hypermutation)를 이용하여 유전자 라이브러리를 진화시키는 방법을 첨가한 동적 클론 선택 알고리즘을 소개한다.

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A Study on Effects of Operating Temperatures of Cyclone on Pressure Drop (사이클론의 작동온도가 압력손실에 미치는 영향에 관한 연구)

  • Kwak, Ji-Young;Song, Myung-Jun;Lee, Yeol
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2012.05a
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    • pp.515-519
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    • 2012
  • A numerical study was performed to observe the relation between internal flow characteristics and operating temperatures in a Lapple cyclone. The present numerical results agree reasonably well with previous experimental and numerical results. As the operating temperature increases, the pressure drop decreases due to decrease of wall shear stress and tangential velocities. A detailed comparison of pressure drops in the cyclone between theoretical prediction models and the present numerical simulations is also presented for wide range of operating temperatures.

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Production of Monoclonal Antibody against Sulfamethazine and Development of ELISA

  • C.S. Chae;Lee, M.H.;Lee, H.;S.Y. Yoon;P.D. Ryu
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.176-176
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    • 1996
  • 축산식품중에 잔류하고 있는 sulfamethazine을 검출하기 위하여 sulfamethazine에 대한 단클론항체를 생산하고 이를 이용하여 효소면역측정법을 개발하였다. 면역원은 sulfamethazine에 KLH를 그리고 흡착항원은 BSA를 glutaraldehyde법으로 결합시켰다. 면역원으로 Balb/c mouse를 면역시킨 다음 비장 형질세포률 얻어 myeloma cell과 융합하여 융합잡종세포를 만들었다. Sulfamethazine에 대한 항체를 분비하는 융합잡종세포를 단계회석법과 ELISA를 이용하여 cloning하여 D2, A9, B8, Bl 클론을 얻었다. 이들 클론에서 얻어진 단클론항체를 사용하여 indirect competitive ELISA를 실시하여 표준곡선을 작성하여 본 결과 농도의존성 곡선을 얻을 수 있었다. 4클론중에서 A9 클론을 사용하여 다른 유사한 sulfonamide듣과 p-aminobenzoic acid와 교차반응을 조사한 결과 sulfamerazine에 12.5%의 교차반응을 보였으나 다른 설파제에 대해서는 교차 반응을 보이지 않았다.

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Numerical Study on the Internal Flow in the Cyclone Vacuum Cleaner (사이클론 청소기 내부 유동에 관한 수치해석적 연구)

  • Lee, Chanhyun;Chang, Hyuksang
    • Clean Technology
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    • v.20 no.3
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    • pp.283-289
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    • 2014
  • General household vacuum cleaners consist of dust collector, pre filter, motor and exhaust filter, and the filtered clean air is discharged to the atmosphere. By using the CFD methods, we estimated the internal flow in two types of commercial cyclone vacuum cleaners to evaluate the dust collection performance. From the analysis, it was known that the number of revolution had higher values in cyclone cone region. CFD analysis in a specific showed non-uniform velocity distribution at outlet, which results in the deterioration of particle collection performance. In order to improve flow condition, the installation of baffle was proposed and the values of velocity RMS were estimated.

A Study on Improving Environmental Characteristics of Cyclone Vacuum Cleaner using Life Cycle Assessment (LCA 평가를 이용한 싸이클론 진공청소기의 친환경성 개선방안에 관한 연구)

  • Hwang, Bo-Seok;Yoon, Yong-Han;Lee, Chanhyun;Yi, Hwa-Cho
    • Clean Technology
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    • v.20 no.3
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    • pp.241-250
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    • 2014
  • In this study, performance and environmental characteristics of cyclone vacuum cleaners are analyzed and product improvement methods are investigated to minimize environmental effect of the cleaners using the result. A simplified LCA method is used to analyze environmental characteristics of the cyclone vacuum cleaners. Two cyclone vacuum cleaners with similar specifications are chosen for the experiment. Typical characteristics of cyclone vacuum cleaners such as energy consumption, suction force, noise and temperature are measured and compared. Most environmental effect was caused by the energy consumption in use phase of life cycle. Some ideas are created to reduce energy consumption of the vacuum cleaners in use phase like installing baffle, and methods to extend exchange period of filter. It is analyzed how recyclability rate of vacuum cleaners could be improved to reduce the environmental effect in whole life of the vacuum cleaners.

The Effect of Cyclone Vortex Finder Configurations on the Fine Particle Collection Efficiencies (사이클론 선회류 약화기 구조에 따른 미세먼지 집진효율 연구)

  • Jeong, Dong Gyun;Kim, Beom Seok;Hong, Min Sun
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.6
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    • pp.371-376
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    • 2017
  • The effect of vortex finder confiqurations on the PM-10 collection efficiencies has been investigated using 7 different types of cyclones. Cyclone inlet velocities were calculated by computational fluid dynamics analysis and PM-10 collection efficiencies were measured from experimental apparatus. The inlet velocities of normal, P and A type cyclones were calculated 15.48 m/sec, 16.03 m/sec and 15.9 m/sec, respectively while experimental results show that PM-10 collection efficiencies were increased 4% for P type and 7% for A type cyclones compared to normal cyclone. Also it was found that there exist optimum parallel head lengths for both P and A type cyclones to maximize the PM-10 collection efficiencies.

Evaluation of Agronomic Characteristics Indigenous Germplasm for Development of High Quality and Yield of Ginger (Zingiber officinale Rosc.) (고품질 다수성 생강 품종육성을 위한 국내 유전자원의 농업적 특성 평가)

  • 최재을;김정선;장원석
    • Korean Journal of Plant Resources
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    • v.12 no.1
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    • pp.38-42
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    • 1999
  • To obtained the basic information needed in the development of higher quality and yield of ginger, twenty-four indigenous colones of ginger were evaluated major agronomic characteristics under field and polyethylene house culture. There was highly significant differences in yield and 6-gingerol contents among the clones studied. Maximum yield was recorded in clone CG9579(3520kg/10a) followed by CG95134, CG9512, CG96109, CG9596, in field culture. The maximum 6-gingerol percentage was recorded in the clone CG9579 containing 4.44mg/g, and next was CG9583 4.35mg/g, CG95134 4.32mg/g DW. In addition, CG9503 and CG95105 clones showed more than 4,000kg/10a in polyethylene house culture.

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Filtration of dispersed nanoparticles using cyclone and ultrasonic atomization (초음파 무화 및 사이클론을 이용한 분산된 나노입자의 여과법)

  • Jung, Jihee;Kim, Moojoon;Kim, Jungsoon
    • The Journal of the Acoustical Society of Korea
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    • v.41 no.1
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    • pp.9-15
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    • 2022
  • In order to overcome the limitation of conventional nanoparticle dispersion methods such as sonicator or homogenizer, a filtration method using an ultrasonic atomization effect and cyclones was proposed in this study. A 0.5 wt% suspension was made with Al2O3 powder with an average diameter of 250 nm. The suspension was filtered by the proposed method after pre-dispersing using a sonicator and a homogenizer, respectively. As the result, in the case of the suspension after pre-dispersing with a sonicator, the particle size distribution of the filtered suspension started showing up a single normal distribution indicating the mode of the average diameter only after the 3rd cyclone process. On the other hand, in the case of the suspension with the homogenizer pre-dispersion, similar results appeared from the 2nd cyclone process. Filtration of various types of nanoparticles is expected to be possible by adjusting ultrasonic atomization frequency and manipulating the design of the cyclone.

Clonal Variation in Female Flowering of Larix leptolepis (낙엽송 클론의 암꽃 개화량 변이)

  • Kim, In-Sik;Kim, Jong-Han;Kang, Jin-Taek;Lee, Byung-Sil
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.1-4
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    • 2008
  • The clonal variation in female flowering was studied in Larix leptolepis clone bank, consisting of 116 clones, for three years. The between-year variation was large; i.e. the percentage of flowering grafts and average number of flowering per graft were $28.4{\sim}67.2$ and $9{\sim}176$, respectively. Differences in flowering abundance among clones were large and statistically significant in all the years studied. The variance of flowering abundance among clones was increased when flowering was poor. The average of broad-sense heritability of flowering abundance was 0.52. The genetic gain(%G) was estimated at 57.4% when the upper 30% clones were selected. The clonal stability of flowering abundance was compared using average number of flowering and coefficient of variance value of each clone. The clones such as Gyeonggi 9(29), Kangwon 37(137), Chungnam 6(46), Chungnam 14(414), R11, R8 showed abundant flowering and high stability.

Initial Root Development of Larix leptolepis Gordon Cuttings as Related to Organic Substances and Cutting Date (낙엽송(落葉松)의 삽목발근(揷木發根)에 관련된 물질(物質) 및 발근과정(發根過程))

  • Koo, Yeong Bon;Hyun, Jung Oh
    • Journal of Korean Society of Forest Science
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    • v.85 no.2
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    • pp.300-308
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    • 1996
  • This study was conducted to identify factors involved in rooting of Japanese larch cuttings. Cuttings were taken every week from June 24th to July 25th before and after the 10th of July which supposed to be best time for cutting. The content of IAA, carbohydrate, nitrogen and inorganic elements such as $Mg^{{+}{+}}$, $K^+$ and $Ca^{{+}{+}}$ within cutting materials were analyzed. After the cuttings were planted, the root development was observed every week. Cuttings of easy-to-root clones were higher in IAA contents than the cuttings of hard-to-root clones at the time of cuttings were taken. There were significant differences in moisture content of cuttings taken on June 24th and July 25th between easy-to-root and hard-to-root clones, however no significant differences in moisture contents were observed between them taken on the 8th and 15th of July which supposed to be the best time for cutting. Inorganic matters and carbohydrate contents of cuttings taken from hedged trees were higher than those of non-hedged trees. The ratios K/C and C/N of cuttings taken from hedged trees were higher than those of non-hedged trees. Cuttings taken from easy-to-root clones showed higher content of carbohydrate and nitrogen than those of hard-to-root clones. The contents of inorganic elements, such as $Mg^{{+}{+}}$, $K^+$, and $Ca^{{+}{+}}$ in cutting taken from hard-to-root clones were slightly higher than that of easy-to-root clones. The ratio C/N and K/C of cuttings which were taken from easy-to-root clones have shown a similar trend of carbohydrate and nitrogen. These data indicate that there was no one dominant factor that affect rooting of cutting, but many factors such as contents of IAA, carbohydrate, nitrogen, phosphate, and ratios of C/N and K/C appeared to affect on rooting of cuttings variously. Most of root initials were formed from phloem tissue within one week after cutting, and few were formed from callus tissue. Root primordia were developed from root initials within two weeks after cutting, and most of new roots were developed from root primordia within about five weeks after cutting.

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