• 한국미생물·생명공학회지
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• 제21권5호
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• pp.406-413
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• 1993

This study was performed to produce monoclonal antibodies to the major polyhedral inclusion body (PIB) antigen of the occluded form of Hyphantria cunea nuclear polyhedrosis virus (HcNPV). PIB proteinS were purfied from the Spodoptera frugiperda cell infected with HcNPVs. Using the purified PIB protein, BALB/C mice were immunized 3 times with 2 weeks intervals. The spleen were removed and fused with Sp2/0-Ag14, mouse myeloma cells.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2002년도 제25회 KOSCI SYMPOSIUM 논문집
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• pp.149-155
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• 2002

A promising new approach to achieve low pollutants emission and improvement of flame stabilities is tested experimentally using a hybrid cyclone jet combustor employing both premixed and diffusion combustion mode, Three kind of nozzles are used for LNG(Liquified Natural Gas) as a fuel. The combustor is operated by two method, One is ATI(Air Tangential Injection) mode, generated swirl flow by air as general swirl combustor, and the other is PTI(Premixed gas Tangential Injection) mode, The PTI mode consists of diffusion flame of axial direction and premixed cyclone flame of tangential direction in order to stabilized the diffusion flame. The results showed that the stable region of the PTI mode is more larger than the ATI mode. In addition, the reduction of NOx emission in PTI mode, as compared with that for the ATI mode is at least 50% in stable region. Also, even using the low calorific fuel as $CO_2$-blended gas, the cyclone jet combustor has high performance of flame stability.

• 한국산업융합학회 논문집
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• 제23권1호
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• pp.101-106
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• 2020

Along with dust collection efficiency, pressure loss is a very important cyclone operation factor. A severe rise in pressure loss causes the problem of cost. To solve the problem, the method connecting axial-vane type cyclones in parallel is suggested recently. The axial vane type cyclone dust collector applied in this study is a small portable type. Multiple cyclones are installed in a round type. The basic performance test on the axial vane type cyclone dust collector was conducted. As a result, the cut size reduced along with a rise in the wind velocity of the cyclone dust collector inlet. According to the test on dust collection efficiency, the effect of dust collection began to appear in the range of 3㎛ and dust collection efficiency was greatly improved at 5 ㎛. The noise of the cyclone dust collector well met the fan sound power level of KSB 6361.

• 에너지공학
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• 제9권3호
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• pp.250-260
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• 2000

유동층 연소기술은 다양한 연료의 사용과 SOx와 같은 공해물질의 배출제어가 용이하여 환경규제에 대한 대처능력이 우수한 발전방식으로 널리 적용되고 있는 추세이다. 국내에서도 동해화력 1,2호기(200MWe급)가 유동층 연소방식을 도입하여 운전중에 있으며, 국내 무연탄을 연료로 사용하는 최대 규모의 순환 유동층 보일러라 할 수 있다. 본 논문에서는 IEA-CFBC 모델을 이용하여 개발된 동해화력 순환유동층 성능모사 시뮬레이션 틀을 이용하여 여러 가지 운전변수 변화에 따른 성능예측을 수행하였으며, 특히, 연소로의 성능향상을 위한 방안의 일환으로 사이클론 개조를 수행하고 그에 따른 성능모사를 고찰하였다. 본 성능평가 결과 연소로내 전체 차압을 증가시키는 운전을 지향하는 것이 연소로내 희박상의 온도분포를 낮추고, 탈황효율을 증가시켜 운전 안정화에 기여하는 것으로 나타났다. 또한, 본 시뮬레이션 틀을 이용한 운전변수 변화에 대한 성능예측 결과 동해화력 운전 실측치와 잘 일치하는 것으로 고찰되었다.

• 한국대기환경학회지
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• 제12권1호
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• pp.43-53
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• 1996

Numerical simulation was performed for the 3-dimensional flow filed of gas and particle phase for cyclone dust collector. FVM(Finite Volume Method) was employed for gas phase. The flow was solved suing the k-.varepsilon. epsilon turbulence model. The particle exit at the bottom of the cone was treated as a solid wall in this model because the gas flow through the effective dust exit is usually insignificant. The major parameters considered in this study was vortex finder diameter, effective dust exit diameterm vortex finder length, inlet type for dimension performance. Particle trajectory calculations were made for three different, particle sizes of 1, 25 and 50 .mu.m. The results obtained from this study give some physical insight of dust particle collection mechanism together with the indication of the collection efficiency. The simulation results were in generally good agreement with empirical knowledge. The application of this kind of computer program looks promising as a potential tool for the design of cyclone and determination of optimum operating condition.

• 한국대기환경학회지
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• 제15권2호
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• pp.201-210
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• 1999

Cyclones have been extensively used in the industry for removing fine particles from the gaseous streams, based on simplicity in design and construction in association with low cost and flexibility to operate in extreme and harsh environments. However, industrial cyclones are typically not very efficient for particles smaller than 10μm. In this work, in order to improve the separation efficiency of reverse flow cyclones, a simple device named Post Cyclone(Poc) in installed on the top of an existing cyclone. Thereby the residual swirl present at the outlet (vortex finder) of a conventional cyclone has been used to capture the escaped dust from the cyclone in the PoC. The performance of PoC was closely evaluated by changing configuration of the PoC and operation condition. In addition, the dust behaviour in th PoC was investigated based on the hypothesis of residual vortex.

• KSBB Journal
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• 제5권4호
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• pp.365-371
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• 1990

To improve the growth of mouse hybridoma 2c3.1 secreting anti-Hepatitis B surface antigen monoclonal antibody (anti-HBsAg MAb), we had constructed an enriched medium and observed the effects of fetal bovine serum and serum-free supplements including human serum albumin, 'insulin and transferrin', and monoethanolamine. For further enhancement of growth, the concentrations of two major energy sources, glucose and glutamine, were strengthened with various ratios in the enriched medium. Maximum cell growth and monoclonal antibody production obtained in various ratios of glucose/glutamine with an inoculation concentration of 2$\times$105 cells/ml were 0.73$\times$106-4.62$\times$106 cells/ml and 65.1-422.6 $\mu\textrm{g}$/ml, respectively. Glutamine was round to be a major energy source and a limiting nutrient in comparison to glucose for 2c3.1 cell cultivation in enriched media with low serum.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2016년도 춘계 학술논문 발표대회
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• pp.64-65
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• 2016

According to the recent increase in the height of supertall buildings, construction lift became one of most important equipment for vertical transportation of resources. However, increase in lifting load during peak time in which the resources are concentrated often causes a risk of construction delay. This study suggests a concept of Double-Cage construction lift, which is a lift with two cages attached together allowing transportation of resources on two consecutive work floors simultaneously. The aim of this study is to present a simulation model suitable for calculating hoisting time of Double-Cage construction lift. The proposed model is expected to be utilized when applying Double-cage construction lift for its efficient operation and management.

• 한국자원리싸이클링학회:학술대회논문집
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• 한국자원리싸이클링학회 2003년도 추계정기총회 및 국제심포지엄
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• pp.241-245
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• 2003

Thiourea 용액에서 금의 고속 전해채취를 위해 사이클론 전해조를 사용하여 금의 회수에 대한 연구를 수행하였다. 금 전해채취의 전해거동을 알기 위해 전해질의 유속, 인가전압, 초기 농도 등의 변수에 대한 실험을 하였다. 기초실험을 통하여 얻은 최적조건 조업하에서 2시간 이내에 전해액 중에 존재하는 99%이상의 금을 회수할 수 있었다.

• Journal of Plant Biology
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• 제39권1호
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• pp.9-13
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• 1996

A clone, LCM1, which encodes calmodulin (CaM) was isolated and characterized from monocot lily (Lilium longiflorum Thunb.) plants. The clone is 681 bps and contains the 447 bp coding region, 8 bp leader sequence, 210 bp 3'-untraslated region, and a poly(A) tail. The coding region of 149 amino acids encodes a protein of predicted Mr 17 kD. Comparison of the LCM1 amino acid sequence with other CaMs revealed that the protein is highly conserved among various living organisms. The expression level of calmodulin gene in lily was studied by RNA blot analysis. The LCM1 mRNA was present in all tissues tested. However, a higher level of calmodulin was observed in anther and floral bud. The level of calmodulin mRNA in anther was about 10 times higher than that in anther was about 10 times higher than that in vegetative tissues. The anther preferential expression of CaM in lily is currently investigated in dicot plants.