• Journal of agriculture & life science
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• v.46 no.5
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• pp.47-55
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• 2012

Polyclonal antisera against regional (abdominal and subcutaneous) fats were developed to reduce body fat in rats. Isolation and culture of abdominal and subcutaneous adipocytes of rats were performed for analyzing lactate dehydrogenase (LDH) concentration. At the level of 1:1,000 dilution, little antibody reactivity appeared in non-immunized serum whereas both of antisera against abdominal (AAb) and subcutaneous adipocyte plasma membrane proteins (SAb) had relatively strong reactivity till the level of 1:128,000 dilutions. Compared with regional fats, extremely low reactivities of AAb and SAb were detected with PMP of the organs (p<0.001). Both AAb and SAb were most strongly reacted with each adipocyte plasma memebrane proteins and showed statistically (p<0.01) higher cross-reactivities compared with non-immunized serum based on LDH analysis. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte plasma membrane proteins are well developed and have safety in cross-reactivities with body organs.

• Korean Journal of Heritage: History & Science
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• v.47 no.4
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• pp.92-105
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• 2014

Microbial characteristics of bacterial population were investigated in human remains and soil inside the bones in excavated grave no.4 and no.5 at Keundokgol site, Osu-ri, Buyeo. Phylogenetic characteristics of bacterial populations were analyzed by direct extracting of ancient DNA. In this study, based on the 16S rDNA sequences, in case of grave no.4, 319s from human remain were classified into 11 phyla, and 462s from soil were classified into 16 phyla. In case of grave no.5, 271s from human remain were classified into 10 phyla, and 497s from soil were classified into 11 phyla. Especially, Actinobacteria phylogenetic group are dominant group of bacterial populations in grave no.4 and no.5. Also, most of these were analyzed uncultured group. Thus, the discovery of a diversely microbial community and uncultured group was thought to be due to the specificity of the sample. Conclusively the general excavated human bones were contaminated with soil bacteria species their near around. This results contribute to preservation and management of ancient human bone from archaeological sites.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.207-212
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• 2022

The optimal culture conditions for root organogenesis from the callus of peonies (Paeonia lactiflora Pall.) were investigated. Root explants with vascular bundles were cultured in Murashige and Skoog (MS) medium combined with 0.5-4.0 mg/L auxins (indole acetic acid [IAA], naphthalene acetic acid [NAA], indolebutyric acid [IBA], and 2,4-dichlorophenoxyacetic acid [2,4-D]) and 0.0-2.0 mg/L cytokinins (kinetin, zeatin, and benzylaminopurine [BAP]) to induce callus formation. The callus was then cultured in MS medium combined with three concentrations (0.1, 0.5, and 1.0 mg/L) of IAA, NAA, IBA, kinetin, zeatin, and BAP in the dark for 6 weeks. Based on the results, the effects of dark and light conditions on the callus cultured in MS medium with combinations of 0.1-1.0 mg/L IBA and zeatin for 6 weeks were studied. Callus formation was most effective (>+++) in the medium with a combination of 1.0 mg/L NAA and 1.0 mg/L zeatin. A high number of long adventitious roots were observed in the mediums with 0.1 mg/L IBA (6.66 and 4.82 cm) and 0.5 mg/L zeatin (2.32 and 0.72 cm) among auxins and cytokinins, respectively. The highest number (14.06) of adventitious roots were formed from the callus cultured in light in the MS medium combined with 0.1 mg/L IBA and 0.5 mg/L zeatin. This same medium induced the formation of the longest adventitious root (5.45 cm) in the dark. Thus, optimization of in vitro culture conditions may be possible for the mass propagation of adventitious roots in peonies.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.517-525
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• 2023

The emergence and spread of multidrug-resistant Pseudomonas aeruginosa (MRPA) have become a serious problem worldwide. The involvement of metallo-β-lactamases (MBLs) in inducing carbapenem resistance is particularly acute. However, unlike other members of the Enterobacteriaceae genus, new clones of P. aeruginosa are constantly emerging and rapidly replacing previously prevalent dominant clones. Therefore, this study aimed to perform antimicrobial resistance gene analysis, integron gene cassette analysis using DNA sequencing, and plasmid transfer analysis by conjugation to investigate the antimicrobial resistance dynamics of 18 P. aeruginosa strains isolated from various medical samples at a general hospital in Busan from September 2017 to September 2019. All 18 strains showed extensively drug-resistant (XDR) phenotype and were resistant to most antibiotics, except colistin (100%) but were susceptible to aztreonam (22.2%) and ceftazidime (16.6%). Approximately 66.7% of the strains had Class 1 integrons showing various antimicrobial resistances. Notably, IMP-6 ST235 (66.7%), VIM-2 ST357 (16.7%), and IMP-1 ST446(16.7%) were identified. The identification of IMP-1-producing ST446, previously unreported in Korea, is noteworthy considering the emergence and prevalence of another MRPA high-risk clone.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 1993.03a
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• pp.14.1-14
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• 1993

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.07a
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• pp.427-433
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• 2003

숙주세포가 분열될 때, 재조합 DNA 분자의 복제물이 자손에게 전달되며 거기서 벡터 복제가 일어난다. 수많은 세포 분열이 일어난 후, 동일한 숙주세포의 콜로니(colony), 혹은 클론(clone)이 생성된다. 이때 숙주세포의 분열은 배양액을 담은 용기에서 이루어진다. 박테리아 속의 배양된 콜로니에 재조합 DNA가 포함되어 있지 않으면 푸른색을 띠고, 재조합 DNA가 포함되어 있으면 흰색을 띠게된다. 이 흰색의 콜로니만을 추출해 내는 것이 바로 콜로니 픽킹(picking)이라 한다. (중략)

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.713-714
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• 2000

Six monoclonal antibodies to human cardiac troponin I (hcTnI) were produced to eventually develop an immunosensor for acute myocardial infarction (AMI). For the characterization of these antibodies, a set of 11 different peptides covering selected ranges of the complete amino acid sequence of hcTnI was prepared and used for epitope mapping. Such analysis allowed to select an appropriate pair of antibodies that can form a sandwich type of immune complexes and was consequently used for an immunoassay.

• Journal of Life Science
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• v.2 no.1
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• pp.26-34
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• 1992

유전자 재조합 균주의 발효에 있어서 플라스미드의 안정성과 발효공정의 최적화에 대해 개략적으로 서술하였다. 클론된 DNA의 발현은 플라스미드의 안정성을 크게 저해하며, 저하된 플라스미드의 안정성은 재조합 균주의 생산성을 많이 떨어뜨린다. 최적 발효 조건은 각각의 숙주-벡터 시스템, 사용한 배지, 생성물 등에 따라 크게 변한다. 동일한 숙주-벡터 시스템의 경우도, 사용하는 배지에 따라 온도, 희석률 또는 성장속도에 의존하는 정도가 달라지고 또 최적값도 다 변한다. 또한 발효조건의 최적화가 균체 내 플라스미드의 자기복제,mRNA로의 전사, 단백질로의 translation, 더 나아가 미생물 전체의 생리와 밀접하게 관련이 있다.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.04a
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• pp.213-214
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• 2000

입자상 물질을 제거하기 위한 수단으로는 싸이클론, 필터, 전기집진기, 세정탑 등이 있다. 이중에 세정탑은 설치 및 운전이 편리한 반면, 미세 입자 제거 효율이 낮아 산업체에서 제한적으로 사용되고 있다. 이러한 결점, 즉 미세 입자의 제거 효율을 높이기 위해 다양한 형태의 세정탑이 연구되었으나(Laitinen et al., 1997; Fan et al., 1988), 대부분 실험적인 연구로서 세정탑에서 입자의 제거 기작이나 크기 분포 특성을 설명하지는 못한다. (중략)

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.11a
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• pp.497-498
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• 2003

cyclone은 원심력을 이용하여 입자를 포집하는 집진장치로 간단한 구조 때문에 입자의 전처리 장치로 많이 사용되어 왔다. 그러나 미세 입자의 제어가 어렵고, 포집 효율의 증가를 위해 유량을 증가시킬 경우 유랑의 증가에 의한 압력손실과 에너지의 손실이 증가하며, 유량을 감소시킬 경우 입자의 포집 효율이 급격히 감소하는 점 등의 단점이 있다. 따라서, 미세 입자에 대한 포집 효율을 증가시키기 위해 싸이클론의 구조 및 형채를 변화시키는 많은 연구가 수행된 바 있다(Lim et al., 2001; Lim et al., 2003). 본 연구에서는 granule에 의한 관성충돌 효과를 이용하여, 일반적인 cyclone의 설계를 목적으로 하였다. (중략)