• Journal of Aquaculture
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• v.18 no.4
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• pp.225-230
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• 2005

This experiment was conducted to compare the effects of extruded pellets and raw fish-based pellet on olive flounder Paralichthys. olivaceus. Six diets were prepared for this study: two formulated extruded pellets (FEP1 & FEP2), three commercially available extruded pellets (CEP1, CEP2 & CEP3) and moist pellet (MP). Weight gain offish fed FEP1 and CEP3 were significantly higher (P<0.05) than those of fish fed FEP2, CEP1, CEP2 and MP, while that of fish fed MP was not significantly different (f<0.05) from those of fish fed the FEP2, CEP1 and CEP2. Feed efficiency of fish fed CEP2 was significantly lower (P<0.05) than those of fish fed FEP1, FEP2, CEP1, CEP3 and MP. There was no significant difference in protein efficiency ratio and hepatosomatic index between fish fed FEP1 and CEP3, and among fish fed FEP2, CEP1 and CEP2. There was no significant difference in condition factor among fish fed the FEP1 and CEP3, and between fish fed FEP2, CTP1 and MP. However, fish fed MP had a lower survival rate than fish fed the other five EP These results suggest that diet FEPl could be developed to replace MP for the owing stage of flounder without adverse effects on growth performance.

• Journal of Korean Society of Forest Science
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• v.106 no.3
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• pp.310-319
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• 2017

This study categorized the damaged trees by Supervised Classification using time-series-aerial photographs of Bukhan, Cheonggae and Suri mountains because oak wilt disease seemed to be concentrated in the metropolitan regions. In order to analyze the spatial characteristics of the damaged areas, the geographical characteristics such as elevation and slope were statistically analyzed to confirm their strong correlation. Based on the results from the statistical analysis of Moran's I, we have retrieved the following: (i) the value of Moran's I in Bukhan mountain is estimated to be 0.25, 0.32, and 0.24 in 2009, 2010 and 2012, respectively. (ii) the value of Moran's I in Cheonggye mountain estimated to be 0.26, 0.32 and 0.22 in 2010, 2012 and 2014, respectively and (iii) the value of Moran's I in Suri mountain estimated to be 0.42 and 0.42 in 2012 and 2014. respectively. These numbers suggest that the damaged trees are distributed in clusters. In addition, we conducted hotspot analysis to identify how the damaged tree clusters shift over time and we were able to verify that hotspots move in time series. According to our research outcome from the analysis of the entire hotspot areas (z-score>1.65), there were 80 percent probability of oak wilt disease occurring in the broadleaf or mixed-stand forests with elevation of 200~400 m and slope of 20~40 degrees. This result indicates that oak wilt disease hotspots can occur or shift into areas with the above geographical features or forest conditions. Therefore, this research outcome can be used as a basic resource when predicting the oak wilt disease spread-patterns, and it can also prevent disease and insect pest related harms to assist the policy makers to better implement the necessary solutions.

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.12-22
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• 2001

Purpose: The ictal perfusion patterns of cerebellum and basal ganglia have not been systematically investigated in patients with temporal lobe epilepsy (TLE). Their ictal perfusion patterns were analyzed in relation with temporal lobe and frontal lobe hyperperfusion during TLE seizures using SPECT subtraction. Materials and Methods: Thirty-three TLE patients had interictal and ictal SPECT, video-EEG monitoring, SPGR MRI, and SPECT subtraction with MRI co-registration. Results: The vermian cerebellar hyperperfusion (CH) was observed in 26 patients (78.8%) and hemispheric CH in 25 (75.8%). Compared to the side of epileptogenic temporal lobe, there were seven ipsilateral hemispheric CH (28.0%), fifteen contralateral hemispheric CH (60.0%) and three bilateral hemispheric CH (12.0%). CH was more frequently observed in patients with additional frontal hyperperfusion (14/15, 93.3%) than in patients without frontal hyperperfusion (11/18, 61.1%). The basal ganglia hyperperfusion (BGH) was seen in 11 of the 15 patients with frontotemporal hyperperfusion (73.3%) and 11 of the 18 with temporal hyperperfusion only (61.1%). In 17 patients with unilateral BGH, contralateral CH to the BGH was observed in 14 (82.5%) and ipsilateral CH to BGH in 2 (11.8%) and bilateral CH in 1 (5.9%). Conclusion: The cerebellar hyperperfusion and basal ganglia hyperperfusion during seizures of TLE can be contralateral, ipsilateral or bilateral to the seizure focus. The presence of additional frontal or basal ganglia hyperperfusion was more frequently associated with contralateral hemispheric CH to their sides. However, temporal lobe hyperperfusion appears to be related with both ipsilateral and contralateral hemispheric CH.

• Korean Journal of Environmental Biology
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• v.30 no.1
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• pp.15-25
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• 2012

From April to November 2009, we performed field investigation to survey the characteristics of fish fauna and fish community structure inhabited in Yongdam reservoir in the upper Geumgang, which is changed into flat-water zone from flow-water zone by blocking the continuity by the gigantic submerged weir built in the upstream of Geumgang. 15 species belonging to 8 families were collected from natural habitat (St. 1) where its natural characteristics is well preserved, and 11 species were korean endemic fish species. 24 species belonging to 10 families were collected at the down region of Yongdam dam (St. 3), which might be affected by the change of water environment due to the dam, and 11 species were korean endemic fish species. On the other hand, 20 species belonging to 7 families were collected inside Yongdam reservoir (St. 2) which is changed into flat-water zone from flow-water zone by the dam reservoir, and 6 species were korean endemic fish species. In the dam reservoir, due to Yongdam dam built in the upper Geumgang, the original flow-water zone fish such as $Acheilognathus$ $koreensis$, $Pseudopungtungia$ $nigra$, $Coreoleuciscus$ $splendidus$, and $Gobiobotia$ $macrocephala$ were disappeared, and instead, the kinds of fish habitating in the flat-water zone tend to increase rapidly, such as $Carassius$ $auratus$, $Opsarichthys$ $uncirostris$ $amurensis$, $Hemiculter$ $eigenmanni$, $Zacco$ $platypus$, and $Lepomis$ $macrochirus$. Relative abundance of the insective fish was 66.7% at St. 1, 40.0% at St. 2, and 54.2% at St. 3. In order to preserve endemic fish species and aquatic ecosystem, it is desirable to minimize the artificial installation in the upper river, such as a large scale dam which can affect the habitat and if inevitable, it is required to prepare preservation measures when building facilities.

• Journal of Environmental Impact Assessment
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• v.21 no.3
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• pp.483-501
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• 2012

The purpose of this study was to apply a multi-metric model of Lentic Ecosystem Health Assessments(LEHA) for environmental impact assessments of Cheongpyung Reservoir during 2005 - 2006 and assessed the ecological model values. The ecosystem model of LEHA was composed of eleven metrics such as biological parameters($B_p$), physical parameters($P_p$), and chemical parameters($C_p$), and determined the rank of ecological health by the criteria. The variables of $B_p$ were metrics of % sensitive species($M_2$, NMS) and insectivore species($M_5$, % $I_n$), which decrease as the water quality degradates, and these metric values were low as 1.5% and 32.4%, respectively. In contrast, the proportions of tolerant species and omnivore species as the other $B_p$ parameters were 43% and 62%, respectively, which indicate a degradation and disturbance of the ecosystem. Riparian vegetation coverage($M_9$, % $V_c$) as a variable of $P_p$, were higher in the 2nd than 1st survey, and decreased toward the dam site from the headwaters. This was due to a habitat simplification(modifications) by frequent bottom dredging of sand and rocks. The variables of $C_p$ were two metrics of specific conductivity($M_{10}$, $C_I$) as an indicator of ionic contents(cations and anions) and the Trophic State Index(TSI) based on chlorophyll-a($M_{11}$, $TSI_{CHL}$) as an indicator of trophic state. These metric values of $C_p$ had high temporal variations, but low spatial variations on the main axis of the reservoir along with the ecological health of a good condition. The environmental impact assessments using the LEHA multi-metric model indicated that the model values of LEHA averaged 30.7 in 1st survey(fair - poor condition) vs. 28 in 2nd survey(poor condition), indicating a temporal variation of the ecological health. The model values of LEHA showed a minimum(28) in the lacustrine zone(S5) and ranged from 29 to 30 in the other locations sampled, indicating a low longitudinal variation. Overall, environmental impact assessments, based on LEHA model, suggest that chemical water quality conditions were in good, but biological conditions were disturbed due to habitat modifications by frequent dredgings in the system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.408-419
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• 2006

A pot experiment was conducted for two years to evaluate the effects of purified Si fertilization combined with $^{15}N$ on the nutrient uptake, plant growth characteristics, and photosynthetic characteristics of rice in water melon cultivated soil. In 2002, plant height was positively affected at 25 DAT (Day After Transplanting) by Si fertilization in 100%N treatment. However, in 2003, plant height at 25 DAT was negatively affected by Si fertilization in low N level but it was reversed in high N level with initial increase of plant height. Tiller number per pot was positively affected by N and Si fertilization level, especially for high N fertilized treatment. Leaf color was positively affected by Si fertilizatlon in no N fertilized pots, however, Si was not effected in 50%N and 100%N fertilized treatments. N harvest index (NHI) increased with increased Si fertilization in no N plots, however it decreased with increasing of N fertilization level. Nitrogen use efficiency (NUE) decreased with increasing of fertilized N but Si fertilization increased NUE in 50%N plots, however, it was not different by the Si fertilization level in 100%N plots. In 50%N+200%Si plots, NUE was greatest with 130 and shoot N content was $16.2g-N/m^{2}$. N content ($g/m^{2}$) in rice plant increased with increasing Si fertilization in no N plots at panicle initiation stage, 50 and 100%N plots at heading stage and all N treatment at harvesting time. This was mostly more efficient in late growth stage than early growth stage. The concentration (%) of P and K increased with increasing N fertilization level at heading and harvesting but it was not significantly different by the Si fertilization treatment except a little decreasing with increasing Si fertilization level at heading. Potassium content was also not significantly related with N fertilization level except increasing with Si fertilization level at panicle initiation stage. Plant Ca content (%) decreased with increasing of Si fertilization at heading stage and Si fertilization increased Ca content at panicle initiation stage and heading stage and it increased with increasing of Si fertilization level. Photosynthetic activity was not directly related with Si fertilization amount, however, Fluorescent factors, Fv'/Fm' and PsII, were positively affected by Si fertilization level. In conclusion, N fertilization in Si 200% fertilized condition should be reduced by about 50% level of recommended N fertilization for rice cropping in green-house water-melon cultivated paddy field. However, improvement of Ps by Si fertilization could not be attributed to Ps activity in the same leaf area but because of increased total leaf area per pot improved fluorescent characteristics.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.210-218
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• 2015

Melon leaves showing yellowing symptoms were analyzed using electron microscopy and RT-PCR for major cucurbit-infecting-viruses (CMV, MNSV, CGMMV, SqMV, WMV, KGMMV, PRSV and ZYMV) reported in Korea, but these viruses were not detected. As the result of further analysis by next-generation sequencing (NGS), the virus was identified as Cucurbit aphid-borne yellows virus (CABYV), and then confirmed by RT-PCR using CABYV-specific primers. When photosynthetic capacity was measured based on chlorophyll fluorescence yield (ChlFY), the leaves of the diseased plants showed $4.09{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, which was one-third of the readings observed for unaffected normal plants ($12.36{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The root functions of plants affected by leaf yellowing symptoms (LYS) was $0.28mg{\cdot}g^{-1}$, about half that measured for the normal unaffected plants ($0.48mg{\cdot}g^{-1}$). Cytological observations revealed that there were no morphological differences in the palisade parenchyma and mesophyll spongy cells of the leaves between the diseased and the normal plants. However, the same leaf cells of the affected plants contained more starch granules compared to those of the normal, unaffected plants. We conclude that the LYS of muskmelon is not merely a physiological disorder but a viral disease caused by CABYV and spread by aphids.

• Korean Journal of Ecology and Environment
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• v.45 no.3
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• pp.263-270
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• 2012

The objectives of the study were to determine fish fauna and compositions during 2010~2011 in Walpyung Conservation Park along with analysis of fish community structures and trophic and tolerance guilds. Total number of species and individuals sampled were 31 and 2667, respectively and dominant species was Zacco platypus (46.6%) and subdominant species was Acheilognathus lanceolatus (8.7%), which were composed of >50% of the total. Total number of Korean endemic species including Microphysogobio yaluensis was 10 and the proportion of the individuals was 8.7%. Also, natural monument No. 454 of Iksookimia choii, which is endangered species and legal protected species, was sampled and the total number of individuals was only three. According to the analysis of fish community structures, species richness index in the mid-stream reach ($M_r$) was 3.145, which is higher than any other stream reaches ($U_r$ and $D_r$). In contrast, the richness index was 2.180 in the up-stream reach ($U_r$), which is minimum among the sampling sites. Species diversity index was 1.785 and 1.975, respectively in the headwater ($U_r$) and mid-stream reach ($M_r$) and the low values in the down-stream reach ($D_r$, 1.660) were due to the influences of pointsource (i.e., road construction) and non-point sources (sporadic agricultural spots). According to analysis of tolerance guilds, the proportion of tolerant species (TS), based on the number of individuals, was composed of 64.2% and sensitive species (SS) was only 3.3%, indicating a predominance of tolerant fishes in the compositions. The proportion of omnivore species (OS), however, was composed of 64.0% and insectivore species (IS) was 29.3%, indicating a predominance of omnivore fishes in the stream. The high proportions of tolerant and omnivore species in this stream indicates that the water quality and physical habitat environments were degradated in this system. For these reasons, especially natural monument and endemic fish species in this region should be protected from the massive constructions and required to provide efficient stream management strategies.

• Journal of fish pathology
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• v.10 no.2
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• pp.125-135
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• 1997

The possibility of identification of families of antibacterial agent residues in fish tissue was studied by disk assay using three test organisms, Bacillus subtilis BGA, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778. In the present method, a simple clean-up procedure was performed to obtain the aqueous solution from homogenized flounder muscle sample(10g) in Mcilvaine buffer. Then, aqueous solution was fractionated into A and B to be used in disk assay by choloroform and Sep-Pak $C_{18}$ cartridge column after being defatted in hexane. The chloroform layer of fraction A was used for the analysis of macrolide antibiotics(ML), sulfa drugs(SA), chloramphenicol(CP), and quinolone antibiotics(QN). Adsorbed materials to Sep-Pak $C_{18}$ of fraction B were also employed for the analysis of penicillins(PC), tetracyclines(TC), and nitrofuran derivatives(NF) Minimun-detectable concentrations by the present method were, $0.1{\mu}g$/g for oxytetracycline, tetracycline, doxycycline, spiramycin and ciprofloxacin, $0.025{\mu}g$/g for erythromycin and ampicillin, $1.0{\mu}g$/g for sodium nifurstyrenate and florfenical, $0.25{\mu}g$/g for sulfamonomethoxie and sulfadimethoxine, $2.5{\mu}g$/g for oxolinic acid and flumequine, and $15{\mu}g$/g for piromidic acid, respectively. Three test organisms showed different sensitivity patterns for each family of antibacterial agent. Sensitivity patterns were B. cereus > B. subtilis > M. luteus for TC and NF, M. luteus > B, subtilis > B. cereus for ML and PC, B. cereus = B. subtilis > M. luteus for CP and QN, and B. subtilis > B. cereus＝M. luteus for SA. The present method utilizing these characteristics could be useful as a routine screening test for the determination of family of antibacterial agent residues in fish tissue.

• Korean Journal of Food Science and Technology
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• v.10 no.4
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• pp.398-403
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• 1978

Squid was dried on the fluidized-bed in the drying chamber filled with solid particles which were also fluidized with hot-air, and effects of the fluidized particles, the squid's height from the grid and the drying temperature on the drying rate and quality of the squid were observed The mechanism of moisture transfer during the falling rate period was also derived. 1. Sodium chloride was found to be the most suitable fluidized particles and at an air velocity of 3.8 m/sec, optimal fluidization state of this particle was obtained. 2. Uniform profiles of temperature were obtained at a point 4 cm above the grid and the location of squid on the fluidized-bed observed to be suitable when it was 4 cm above the grid. 3. At an air velocity of 3.8 m/sec and when the location height of the squid on the fluidized-bed was 4 cm, the optimal temperature for the drying time which is required to reduce the moisture from 80.8% to 18-22% was 8.5 hours. 4. Drying data followed the empirical equation of unsteady state diffusion $log\;(\frac{W-We}{Wc-We})=-m{\theta}$ in the region of the moisture contents measured and the drying constant (m) was calculated as $0.32hr^{-1}$. These results suggested that the migration of moisture during the falling rate period is due to a diffusion type mechanism. 5. The short constant rate period was observed in the early stage and thereafter, drying was controlled by the falling rate period, and the time ratio of the fluidized bed drying to the through circulation drying for reducing the squid's moisture contents to the same level at the same drying temperature was 1 : 1.4 6. Comparisons of fluidized-bed dried squid and sun dried squid in sale showed that there was no significant change in qualities such as external appearance and hydrogen ion concentration of dry product.