• Journal of Mushroom
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• 제21권1호
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• pp.15-21
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• 2023

Volvariella bombycinais an edible mushroom commercially cultivated in Korea and other countries. In this study, the culture characteristics and optimal growth conditions of milky mushroom strains were determined. The growth temperature and pH range of V. bombycinawas extensively investigated between 15 and 35℃ and pH 3-11. For efficient cultivation, 20 types of nutrient sources were selected, specifically, 21 carbon sources, 6 organic nitrogen sources, 7 inorganic nitrogen sources, 13 amino acids, 9 organic acids, and 13 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum temperature and pH were determined to be 30℃ and pH 8.0, respectively. The optimum concentration of medium elements required for mycelial growth of V. bombycinawas determined to be as follows: carbon source, 0.7% Soluble starch; organic nitrogen source, 0.7% Soytone; inorganic nitrogen source, 1.0% (NH₄)₂C₄H₄O₆; amino acid, 0.1% cysteine; organic acid, 0.07% lactic acid; and inorganic salt, 0.07 mM CaCl₂.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 한국생물환경조절학회 1994년도 봄 심포지움 및 학술논문발표요지
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• pp.92-95
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• 1994

과실의 형질을 비파괴적으로 추정하는 방식은 파괴적 추정방식에 비해 연속적으로 측정이 가능하여 생육상태를 상세히 분석할 수 있으며, 같은 과실에 대하여 반복하여 측정할 수 있는 장점을 지닌다는 점에서 생장분석에 유용하게 사용되고 있는 방식이다. 또한 비파괴적 추정은 simulation에 의한 자동화 등에 적용할 수 있어 최적생장조건을 설정하는데 사용되며, 다량의 과실을 자동선별 및 자동수확하는데 이용될 수 있다. (중략)

• Agrochemical news magazine
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• 제15권6호통권123호
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• pp.24-27
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• 1994

93년 냉해는 작물의 생육 및 결실 등 생리작용에 큰 영향을 주었고 병해충의 종류에 따라 최적조건이 되는 특정 병해충발생이 증가하였다. 94년 가뭄은 밭작물 특히 채소류의 경우 진딧물과 함께 바이러스병 발생도 증가하였다. 여기서는 농촌진흥청 발행 ''93 이상기상과 작물피해실태 종합보고서와 ''93 작물보호사업보고서의 내용을 중심으로 하여 ''93냉해와 ''94가뭄에 따른 주요 채소류에서 병해충 발생양상과 대책을 살펴 보기로 한다.

• Journal of the Korean Society of Food Science and Nutrition
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• 제42권3호
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• pp.434-440
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• 2013

This study was conducted to screen the characteristics and alginate-degrading activity of marine bacterium isolated from brown seaweed (Sargassum thunbergii). The results of 16S ribosomal RNA sequence analysis the strain the genus Vibrio and the strain was subsequently named Vibrio sp. PKA 1003. The optimum culture conditions for the growth of Vibrio sp. PKA 1003 were at pH 7, 3% NaCl, $25^{\circ}C$, and 6% alginic acid, with a 48-hour incubation time. A crude enzyme preparation from Vibrio sp. PKA 1003, showed its highest levels of alginate-degradation activity when cultured at pH 9, $30^{\circ}C$, and 6% alginic acid, with a 63-hour incubation time. Thin layer chromatography analyses confirmed that the crude enzyme released monomers or oligomers from sodium alginate, and results from trypsin treatment showed that the alginate degrading activity depends on this enzyme produced by Vibrio sp. PKA 1003. These results suggest that Vibrio sp. PKA 1003 and its alginate-degrading crude enzyme is useful for the production of alginate oligosaccharides.

• Journal of the Korean Society of Food Science and Nutrition
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• 제43권8호
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• pp.1296-1303
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• 2014

This study was conducted to develop predictive models for the growth of Bacillus cereus on carrot treated with slightly acidic electrolyzed water (SAcEW) and ultrasonication (US) at different storage temperatures. In addition, the inactivation of B. cereus by US with SAcEW was investigated. US treatment with a frequency of 40 kHz and an acoustic energy density of 400 W/L at $40^{\circ}C$ for 3 min showed the maximum reduction of 2.87 log CFU/g B. cereus on carrot, while combined treatment of US (400 W/L, $40^{\circ}C$, 3 min) with SAcEW reached to 3.1 log CFU/g reduction. Growth data of B. cereus on carrot treated with SAcEW and US at different temperatures (4, 10, 15, 20, 25, 30, and $35^{\circ}C$) were collected and used to develop predictive models. The modified Gompertz model was found to be more suitable to describe the growth data. The specific growth rate (SGR) and lag time (LT) obtained from the modified Gompertz model were employed to establish the secondary models. The newly developed secondary models were validated using the root mean square error, bias factor, and accuracy factor. All results of these factors were in the acceptable range of values. After compared SGR and LT of B. cereus on carrot, the results showed that the growth of B. cereus on carrot treated with SAcEW and US was slower than that of single treatment. This result indicates that shelf life of carrot treated with SAcEW and US could be extended. The developed predictive models might also be used to assess the microbiological risk of B. cereus infection in carrot treated with SAcEW and US.

• Journal of Bio-Environment Control
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• 제20권4호
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• pp.341-345
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• 2011

This study was aimed to select the optimal mat condition using existing plant-mats for the efficient planting of bedding Chrysanthemum. At fifty days after cutting with Dendranthema grandiflorum 'Ford', root formation among the treatments using eight different mats outstood with the treatment using 10 mm thick coir net, which has medium inserted between mat layers - called C treatment, compared to other treatments; this treatment had the highest values in the plant height and shoot fresh weight, which were 29 cm and 5.6 g, respectively. On the contrary, in 40 days after transplanting root-formed mats to field, 12 mm thick jute net, which has medium inserted between mat layers, had the highest plants compared to other treatments. However, there was no significant difference in shoot weight compared to C treatment. In experiment of different lengths of cut, the results of growth after transplanting showed that 5 cm long cut performed best compared to 3 and 8 cm long cuts.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.71-71
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• 2018

본 연구는 각시톱시네고사리(Dryopteris hangchowensis Ching)의 전엽체 증식 및 포자체 형성을 위한 번식방법을 구명하고자 수행되었다. 각시톱지네고사리의 식물체를 무가온 온실에 재배하면서 포자를 채취하였으며, 수집된 포자는 기내에서 발아시켜 전엽체를 획득한 다음 계대배양하면서 실험의 재료로 사용하였다. 전엽체 대량증식을 위하여 1/4, 1/2, 1배로 조절한 MS배지와 Knop배지를 조성하였다. 이후 전엽체 300mg을 균일하게 다져 조성된 배지에 8주간 배양하였다. 연구의 결과, 전엽체의 생체중은 1/2MS와 1/4MS에서 3.4g으로 가장 많이 증가하였다. 현미경 관찰의 결과, 두 배지 모두 정상적인 전엽체의 형태로 발달하였으며, 생육도 양호하였다. 포자체 형성을 위한 적정 배양토의 혼합조건을 확인하고자 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하였다. 준비된 배양토를 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 후 기내 배양한 전엽체 1g과 증류수를 함께 10초간 분쇄하여 토양표면에 균일하게 분주한 다음 12주간 재배하였다. 연구의 결과, 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v) 비율로 혼합한 토양에서 포트 당 223.7개로 가장 많은 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과, 상기 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 각시톱시네고사리의 전엽체 증식에 적합한 배지는 경제성과 생육수준을 고려하여 1/4MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v)로 혼합한 토양이 적합하다고 판단되었다.

• The Korean Journal of Mycology
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• 제40권4호
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• pp.204-209
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• 2012

In this study, a novel yeast, Y111-5 for Makgeolli manufacture was selected from Nuruk yeasts, and its optimal culture condition were investigated. The Y111-5 strain was identified as Saccharomyces cerevisiae by phylogenetic analysis of 18S RNA sequence. The maximal growth was obtained when the yeast was cultivated at $30^{\circ}C$ for 15 h in the medium containing sucrose 9% and yeast extract 5%.

• Applied Biological Chemistry
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• 제31권3호
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• pp.255-260
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• 1988

The optimum conditions for lactic acid fermentation by Lactobacillus acidophilus in the media containing malt syrup, skim milk or skim milk and malt syrup were studied to develope marketable fermented beverages from barley. The optimum sugar concentration of malt syrup was $10^{\circ}$ Bx. Addition of skim milk to malt syrup enhanced bacterial growth and acid formation. The best flavor of the product was obtained from the media containing 5% skim milk and sterilized at $90^{\circ}C$ for 30 minutes. Optimum temperature and pH for the formentation were $40^{\circ}C$ and 6.0, and bacterial cells grew logarithmically upto 16 hours.

• Research in Plant Disease
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• 제11권2호
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• pp.173-178
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• 2005

Ampelomyces quisqualis 94013 (AQ94013), a hyperparasite, was selected as an effective biological control agent against cucumber powdery mildew. Optimal temperature for mycelial growth of AQ94013 was $26^{\circ}C$, and the optimal pH was 6.5. Conidia of AQ94013 were more Produced on potato dextrose agar (PDA) in darkness than under alternating cycles of 12 hr fluorescent light and 12 hr darkness. Temperature range for spore germination of the fungus was $10\~35^{\circ}C$, and optimal temperature was $20^{\circ}C$. Conidial germination of the fungus began 8 hr after incubation at $24^{\circ}C$. Germination rate of conidia at concentration of $5{\times}10^5\;spores/ml\;and\;5{\times}10^6\;spores/ml$ was higher than at concentration of $5{\times}10^7\;spores/ml$. The best source of carbon and nitrogen for mycelial growth of the fungus were dextrin and neopeptone, respectively.