Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Cultural Characteristics of a Hyperparasite, Ampelomyces quissqualis 94013

중복기생균 Ampelomices quisqualis 94013의 배양적 특성

  • Lee, Sang-Yeob (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Ryu, Jae-Dang (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
  • 이상엽 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 류재당 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 김홍기 (충남대학교 농업생명과학대학 농생물학과)
  • Published : 2005.12.01
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Abstract

Ampelomyces quisqualis 94013 (AQ94013), a hyperparasite, was selected as an effective biological control agent against cucumber powdery mildew. Optimal temperature for mycelial growth of AQ94013 was $26^{\circ}C$, and the optimal pH was 6.5. Conidia of AQ94013 were more Produced on potato dextrose agar (PDA) in darkness than under alternating cycles of 12 hr fluorescent light and 12 hr darkness. Temperature range for spore germination of the fungus was $10\~35^{\circ}C$, and optimal temperature was $20^{\circ}C$. Conidial germination of the fungus began 8 hr after incubation at $24^{\circ}C$. Germination rate of conidia at concentration of $5{\times}10^5\;spores/ml\;and\;5{\times}10^6\;spores/ml$ was higher than at concentration of $5{\times}10^7\;spores/ml$. The best source of carbon and nitrogen for mycelial growth of the fungus were dextrin and neopeptone, respectively.

Ampelomyces quisqualis 94013는 중복기생균으로 흰가루병 생물적 방제용 우수균주로 선발하여, 이 균의 효율적 이용을 위한 배양적 특성을 조사하였다. A. quisqualis 94013의 균사생육 적은은 $26^{\circ}C$이였으며, 균사생육 최적 pH는 6.5였다. 감자한천배지(PDA)배양시 광조건(형광등 12시간주기)보다는 암조건에서 더 많은 포자를 형성하였다. 이 균의 포자발아 온도범위는 $10\~35^{\circ}C$이고, 최적온도는 $20^{\circ}C$이였다. 포자발아는 $24^{\circ}C$에서 8시간 배양 후 시작되었으며, 포자발아율은 포자현탁액의 농도가 $5{\times}10^7/ml$보다 $5{\times}10^5/ml$나 $5{\times}10^6/ml$에서 높았다. 이 균은 탄소원으로는 dextrin을, 질소원으로는 neopepton을 가장 잘 이용하였다.

Keywords

References

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