• Title/Summary/Keyword: 초분

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The Study on Recombinant Protein Production using S. cerevisiae Mutant Y334 Suitable for GAL Promoter (GAL promoter에 적합한 효모변이주 Y334의 회분식 배양에서의 재조합 단백질 발현특성)

  • Gang, Hwan-Gu;Lee, Mun-Won;Jeon, Hui-Jin
    • KSBB Journal
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    • v.14 no.4
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    • pp.476-481
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    • 1999
  • S. cerevisiae mutant(reg1-501, gal1), which cannot use galactose and has alleviated glucose repression level, is used as host for optimizing induction of GAL promoter. The optimum concentration of galactose as inducer for recombinant protein production and the galactose consumption rate have been tested with S. cerevisiae mutant and compared with conventional S. cerevisiae. The extent of glucose repression were investigated for both strain and the degradation pattern of produced foreign protein have been compared in both cases. The effect of pH on foreign protein degradation pattern were studied for both strains. The secetion efficiency of both strains were carried out. Through these experiments, optimum condition of recombinant protein production by GAL promoter using S. cerevisiae mutant (reg1-501, gal1) were found.

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Error Analysis of Linear Mixture Model using Laboratory Spectral Measurements (실내 분광 측정자료를 이용한 선형혼합모델의 오차 분석)

  • Kim, Sun-Hwa;Shin, Jung-Il;Shin, Sang-Min;Lee, Kyu-Sung
    • Korean Journal of Remote Sensing
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    • v.23 no.6
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    • pp.537-546
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    • 2007
  • In hyperspectral remote sensing, linear spectral mixture model is a common procedure decomposing into the components of a mixed pixel and estimating the fraction of each end-member. Although linear spectral mixture model is frequently used in geology and mineral mapping because this model is simple and easy to apply, this model is not always valid in forest and urban area having rather complex structure. This study aims to analyze possible error for applying linear spectral mixture model. For the study, we measured laboratory spectra of mixture sample, having various materials, fractions, distributions. The accuracy of linear mixture model is low with the mixture sample having similar fraction because the multi-scattering between components is maximum. Additionally, this multi-scattering is related to the types, fraction, and distribution of components. Further analysis is necessary to quantify errors from linear spectral mixture model.

Evaluation of Rededge-M Camera for Water Color Observation after Image Preprocessing (영상 전처리 수행을 통한 Rededge-M 카메라의 수색 관측에의 활용성 검토)

  • Kim, Wonkook;Roh, Sang-Hyun;Moon, Yongseon;Jung, Sunghun
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.37 no.3
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    • pp.167-175
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    • 2019
  • Water color analysis allows non-destructive estimation of abundance of optically active water constituents in the water body. Recently, there have been increasing needs for light-weighted multispectral cameras that can be integrated with low altitude unmanned platforms such as drones, autonomous vehicles, and heli-kites, for the water color analysis by spectroradiometers. This study performs the preprocessing of the Micasense Rededge-M camera which recently receives a growing attention from the earth observation community for its handiness and applicability for local environment monitoring, and investigates the applicability of Rededge-M data for water color analysis. The Vignette correction and the band alignment were conducted for the radiometric image data from Rededge-M, and the sky, water, and solar radiation essential for the water color analysis, and the resultant remote sensing reflectance were validated with an independent hyperspectral instrument, TriOS RAMSES. The experiment shows that Rededge-M generally satisfies the basic performance criteria for water color analysis, although noticeable differences are observed in the blue (475 nm) and the near-infrared (840 nm) band compared with RAMSES.

Feasibility Assessment of Spectral Band Adjustment Factor of KOMPSAT-3 for Agriculture Remote Sensing (농업관측을 위한 KOMPSAT-3 위성의 Spectral Band Adjustment Factor 적용성 평가)

  • Ahn, Ho-yong;Kim, Kye-young;Lee, Kyung-do;Park, Chan-won;So, Kyu-ho;Na, Sang-il
    • Korean Journal of Remote Sensing
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    • v.34 no.6_3
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    • pp.1369-1382
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    • 2018
  • As the number of multispectral satellites increases, it is expected that it will be possible to acquire and use images for periodically. However, there is a problem of data discrepancy due to different overpass time, period and spatial resolution. In particular, the difference in band bandwidths became different reflectance even for images taken at the same time and affect uncertainty in the analysis of vegetation activity such as vegetation index. The purpose of this study is to estimate the band adjustment factor according to the difference of bandwidth with other multispectral satellites for the application of KOMPSAT-3 satellite in agriculture field. The Spectral band adjustment factor (SBAF) were calculated using the hyperspectral satellite images acquired in the desert area. As a result of applying SBAF to the main crop area, the vegetation index showed a high agreement rate of relative percentage difference within 3% except for the Hapcheon area where the zenith angle was 25. For the estimation of SBAF, this study used only one set of images, which did not consider season and solar zenith angle of SBAF variation. Therefore, long-term analysis is necessary to solve SBAF uncertainty in the future.

Radiometric Cross Calibration of KOMPSAT-3 and Lnadsat-8 for Time-Series Harmonization (KOMPSAT-3와 Landsat-8의 시계열 융합활용을 위한 교차검보정)

  • Ahn, Ho-yong;Na, Sang-il;Park, Chan-won;Hong, Suk-young;So, Kyu-ho;Lee, Kyung-do
    • Korean Journal of Remote Sensing
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    • v.36 no.6_2
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    • pp.1523-1535
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    • 2020
  • In order to produce crop information using remote sensing, we use classification and growth monitoring based on crop phenology. Therefore, time-series satellite images with a short period are required. However, there are limitations to acquiring time-series satellite data, so it is necessary to use fusion with other earth observation satellites. Before fusion of various satellite image data, it is necessary to overcome the inherent difference in radiometric characteristics of satellites. This study performed Korea Multi-Purpose Satellite-3 (KOMPSAT-3) cross calibration with Landsat-8 as the first step for fusion. Top of Atmosphere (TOA) Reflectance was compared by applying Spectral Band Adjustment Factor (SBAF) to each satellite using hyperspectral sensor band aggregation. As a result of cross calibration, KOMPSAT-3 and Landsat-8 satellites showed a difference in reflectance of less than 4% in Blue, Green, and Red bands, and 6% in NIR bands. KOMPSAT-3, without on-board calibrator, idicate lower radiometric stability compared to ladnsat-8. In the future, efforts are needed to produce normalized reflectance data through BRDF (Bidirectional reflectance distribution function) correction and SBAF application for spectral characteristics of agricultural land.

Possibility for Early Detection on Crop Water Stress Using Plural Vegetation Indices (작물 가뭄스트레스 조기탐지 가능성 타진을 위한 서로 다른 종류의 식생지수 활용)

  • Moon, Hyun-Dong;Jo, Euni;Cho, Yuna;Kim, Hyunki;Kim, Bo-kyeong;Lee, Yuhyeon;Jeong, Hoejeong;Kwon, Dongwon;Cho, Jaeil
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1573-1579
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    • 2022
  • The irrigation schedule system using early detection of crop water stress is required to maintain crop production and save water resource. However, because previous studies focused on the crop under stress dominant condition, the crop physiological properties, which can be measured by remote sensing technique, on early crop water stress condition are not well known. In this study, the canopy temperature, MERIS Terrestrial Chlorophyll Index (MTCI), and Chlorophyll/Carotenoid Index (CCI) are observed on the soybeans given the early water stress using thermal imaging camera and hyperspectral camera. The increased canopy temperature and decreased MTCI are consist with the previous studies which are for the crop of stress dominant-sign. However, the CCI was increased contrary to expectation because it may faster the reduction of carotenoid than chlorophyll in early stage. These behaviors will be useful to not only develop the irrigation system but also using the early detection of crop stress.

A study on the predictability of acoustic power distribution of English speech for English academic achievement in a Science Academy (과학영재학교 재학생 영어발화 주파수 대역별 음향 에너지 분포의 영어 성취도 예측성 연구)

  • Park, Soon;Ahn, Hyunkee
    • Phonetics and Speech Sciences
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    • v.14 no.3
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    • pp.41-49
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    • 2022
  • The average acoustic distribution of American English speakers was statistically compared with the English-speaking patterns of gifted students in a Science Academy in Korea. By analyzing speech recordings, the duration time of which is much longer than in previous studies, this research identified the degree of acoustic proximity between the two parties and the predictability of English academic achievement of gifted high school students. Long-term spectral acoustic power distribution vectors were obtained for 2,048 center frequencies in the range of 20 Hz to 20,000 Hz by applying an long-term average speech spectrum (LTASS) MATLAB code. Three more variables were statistically compared to discover additional indices that can predict future English academic achievement: the receptive vocabulary size test, the cumulative vocabulary scores of English formative assessment, and the English Speaking Proficiency Test scores. Linear regression and correlational analyses between the four variables showed that the receptive vocabulary size test and the low-frequency vocabulary formative assessments which require both lexical and domain-specific science background knowledge are relatively more significant variables than a basic suprasegmental level English fluency in the predictability of gifted students' academic achievement.

IPA Study of Landscape Potentiality of Agricultural and Fishery Heritages - A Focus on Cheongsando - (농어업유산의 경관 잠재력 파악을 위한 IPA 연구 - 청산도를 중심으로 -)

  • Kim, Dong-Chan;Choi, Woo-Young
    • Journal of the Korean Institute of Landscape Architecture
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    • v.42 no.4
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    • pp.76-88
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    • 2014
  • The purpose of this study is to derive landscape characteristic elements of agricultural and fishery heritage and to classify landscape characteristic elements of Cheongsando designated as Korean agricultural and fishery heritage No.1 as well as a Globally Important Agricultural Heritage System (GIAHS). Additionally, this study was conducted to suggest priority for conservation and management of Cheongsando by grasping differences between factors which visitors deem as important and satisfying. To implement this study, the literature review was written, an on-site survey to derive landscape characteristic elements of Cheongsando and a survey on importance and satisfaction of landscape characteristic elements of Cheongsando were conducted, and reliability analysis, descriptive statistical analysis and IPA analysis were performed using SPSS 20.0. The results are as follows: The analysis showed the factors requiring persistent efforts in the first quadrant are the rape flower garden, the sea, green barley field, flat stone paddy field, breakwater and lighthouse, abalone farms, stone houses, thatched houses, a coastal road, the slow road, Stonewall Walkway of Seongseo Village, and residents' agricultural behaviors. The analysis showed the factors needing intensive management strategies in the second quadrant are the surrounding mountain area, dock, Docheong Harbor, vessels, fish market, Doksari stone wall, garish-roofed farm villages, excursion school to a slow island, pension and cafe, bus stop, shade trees, Raw Fish Street, the beach and the filming site. Analysis indicated that the factors needing management control in the third quadrant are the pine grove, the beach, tidal mudflat, the garlic fields, vinyl greenhouses, grain drying yard, sea mustard drying yard, heritage center, Choboon, Dangri exorcism, the market place, residents' fishery behaviors, residents' industrial behaviors, residents' ordinary behaviors, visitors' behaviors that visiting the dock, visitors' behaviors that walking the slow road, visitors' behaviors that eating and shopping for specialties, visitors' behaviors that experiencing agriculture and fishery. Excessive effort factors in the fourth quadrant were not derived.

Investigation of O4 Air Mass Factor Sensitivity to Aerosol Peak Height Using UV-VIS Hyperspectral Synthetic Radiance in Various Measurement Conditions (UV-VIS 초분광 위성센서 모의복사휘도를 활용한 다양한 관측환경에서의 에어로솔 유효고도에 대한 O4 대기질량인자 민감도 조사)

  • Choi, Wonei;Lee, Hanlim;Choi, Chuluong;Lee, Yangwon;Noh, Youngmin
    • Korean Journal of Remote Sensing
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    • v.36 no.2_1
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    • pp.155-165
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    • 2020
  • In this present study, the sensitivity of O4 Air Mass Factor (AMF) to Aerosol Peak Height (APH) has been investigated using radiative transfer model according to various parameters(wavelength (340 nm and 477 nm), aerosol type (smoke, dust, sulfate), aerosol optical depth (AOD), surface reflectance, solar zenith angle, and viewing zenith angle). In general, it was found that O4 AMF at 477 nm is more sensitive to APH than that at 340 nm and is stably retrieved with low spectral fitting error in Differential Optical Absorption Spectroscopy (DOAS) analysis. In high AOD condition, sensitivity of O4 AMF on APH tends to increase. O4 AMF at 340 nm decreased with increasing solar zenith angle. This dependency isthought to be induced by the decrease in length of the light path where O4 absorption occurs due to the shielding effect caused by Rayleigh and Mie scattering at high solar zenith angles above 40°. At 477 nm, as the solar zenith angle increased, multiple scattering caused by Rayleigh and Mie scattering partly leads to the increase of O4 AMF in nonlinear function. Based on synthetic radiance, APHs have been retrieved using O4 AMF. Additionally, the effect of AOD uncertainty on APH retrieval error has been investigated. Among three aerosol types, APH retrieval for sulfate type is found to have the largest APH retrieval error due to uncertainty of AOD. In the case of dust aerosol, it was found that the influence of AOD uncertainty is negligible. It indicates that aerosol types affect APH retrieval error since absorption scattering characteristics of each aerosol type are various.

Anti-obesity effect of Korean Hamcho (Salicornia herbacea L.) powder on high-fat diet-induced obese rats (고지방식이로 유도한 비만 흰쥐에서 함초 (Salicornia herbacea L.) 분말의 항비만 효과 탐색)

  • Kim, Mi Joung;Jun, Hyun Young;Kim, Jung Hee
    • Journal of Nutrition and Health
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    • v.48 no.2
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    • pp.123-132
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    • 2015
  • Purpose: The objective of this study was to examine whether Hamcho freeze-dried powder and Hamcho freeze-dried nano powder have an anti-obese effect on high fat diet-induced obese rats. Methods: Sprague Dawley rats were divided into 6 different groups, each of which was bred for 8 weeks with a different experimental diet: normal diet group (NC), high fat diet group (HFC), high fat diet + 2% Salicornia herbacea L. freeze-dried powder group (2% HS), high fat diet + 5% Salicornia herbacea L. freeze-dried powder group (5%HS), high fat diet + 1% Salicornia herbacea L. freeze-dried nano powder group (1% HSN), high fat diet + 2% Salicornia herbacea L. freeze-dried nano powder group (2% HSN). Results: The 5% HS group showed a weight loss effect in body weight, liver and adipose tissue by reducing the amount of dietary intake and food efficiency ratio. In addition, the 5% HS group showed a significant reduction of serum leptin concentration, while having a beneficial effect on the improvement of lipid metabolism such as increase the serum concentration of adiponectin. However, Hamcho freeze-dried nano powder did not show a weight loss effect. Conclusion: Overall data indicated that Hamcho seemed to have anti-obesity effects. In particular, consumption of 5% Hamcho freeze-dried powder might have beneficial effects on body weight, serum adiponectin level, and lipid profiles since it led to reduced body weight and growth of adipose tissues by suppressing dietary intakes. However conduct of further research studies is needed for analysis of active components and biochemical action mechanism of Hamcho.