• Weed & Turfgrass Science
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• v.5 no.1
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• pp.35-41
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• 2016

Earthworm cast is a harmful factor which reduces the uniformity of management of turfgrass and play quality in golf courses and playground. This research was conducted to carry out the efficacy of some pesticide (bifenthrin GR + clothianidin GR, ethoprophos GR, imidacloprid SC, picoxystrobin WG, thiophanate-methyl WP) and tee tree (Camellia oliefera) extracts against reducing earthworm cast in golf courses. All tested materials were acted reducing earthworm cast, thiophanate-methyl among them showed the highest effect reducing earthworm casts in golf courses. Tea tree extracts was occurred similar efficacy among tested chemical pesticides. However, pellet type byproduct of tea seed extract and liquid formulation of tea tree extract were better than granule formulation of them. Byproduct of tea seed and tea tree extract can be used for alternative biological management material for reducing earthworm cast in heavily casting occurrence area.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.339-341
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• 2005

본 연구는 폐기되는 차나무 종자를 이용하여 항종양효과를 비교하고 차나무 잎뿐만 아니라 항종양 효과를 나타내는 차나무 종자의 이용가능성에 대해 탐색하고자 기획하였다. 항종양활성을 검토하기 위하여 2종류의 인체 암세포주(위암 및 폐암)에 대하여 조사하였다. 항종양효과는 종자 물추출물에서는 큰 효과를 나타내었다. 종자 물 추출물에서 분리한 상징액의 항암효과를 측정한 결과, 100 ${\mu}$g/mL 농도에서 위암 세포인 AGS 세포주에 대해 72.8%, 폐암 세포인 A549 세포주에 대해 71.4%의 항종양 효과를 얻을 수 있었고 침전물에서는 종양세포증식억제효과가 나타나지 않았다.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.191-198
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• 2010

Effective sampling method and distribution of earthworms in turfgrasses of golf courses were investigated at 10 Korean golf clubs. Tea tree extract was highly effective in extracting Eisenia andrei compared with mustard oil or formalin. 1000-fold diluent of tea tree extract (4 L/$m^2$) was more effective than 500- and 2000-fold. Sampling efficacy of earthworm increased as soil depth decreased. Additional watering after treatment of tea tree extract did not significantly influence the sampling of earthworms. Eight earthworm species in 4 genera were collected from turfgrasses of 10 golf courses with 4 L of 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$. Among, dominant earthworm was Amynthas heteropodus and collected 147 individuals from 6 golf clubs. Thirteen Drawida japonica was collected from 3 golf clubs and A. hilgendorfi and A. hupeiensis were collected from 2 golf clubs. A. hupeiensis was not collected with 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$.

• Korean Journal of Food Science and Technology
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• v.37 no.1
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• pp.108-112
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• 2005

Antimicrobial and antitumor activities of Camellia sinensis L seed extracts were investigated. Seed extracts showed antifungal activities against Candida albicans IFO 1594 and Cryptococcus neoformans. Inhibition zone of 20 mm was shown by 70% ethanol extract against C. albicans IFO 1594 at 100 mg/mL. Antifungal activity of seed extract was not decreased by heating at 80 and $100^{\circ}C$ for 30 min or at $121^{\circ}C$ for 15 min, indicating heat-stability of seed component. Growth-inhibitory effects were observed in 70 and 10% of tumor cell line SK-OV-3 and normal ceil line NIH/3T3 at $50{\mu}g/mL$, respectively.

• Korean Journal of Organic Agriculture
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• v.26 no.4
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• pp.707-717
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• 2018

The acaricidal activity of extracts of Gleditsia japonica var. koraiensis, Camellia sinensis, Cinnamomum cassia, Lantana camara and their mixtures against the tea red spider mite, Tetranychus kanzawai was evaluated. Treatments with mixtures 3 and 1 were effective against the adult females of T. kanzawai and yielded 82.0% and 77.3% adulticidal activities at 7 days after treatment, respectively. Treatment with mixture 2 revealed 68.0% adulticidal activity. Generally, the acaricidal activity of the single treatment of each plant extract was lower than the mixtures. The adult females of T. kanzawai treated with mixtures 3 and 1 produced only 24.0~29.6% as many eggs as control females did. The residue of the plant extracts tested had low adulticidal activity (16.7~31.3% at 7 days after treatment). All the plant extracts tested exhibited no remarkable toxic effect to the eggs of T. kanzawai. Treatments of mixtures 3 and 1 were effective against nymphs of T. kanzawai and led to emergence rates of 16.7 and 22.0%, respectively. These results suggest that mixtures 3 and 1 might be used for the control of T. kanzawai in tea fields.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.46-51
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• 2019

This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.

• Journal of the Korea Convergence Society
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• v.8 no.12
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• pp.109-117
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• 2017

The purpose of the study aimed to explore the possibility for natural preservative convergence materials by examining the antioxidant activity and antimicrobial effect of 19 wild plants in Jiri Mountain. Total polyphenols were higher in the order of CS(Camellia sinensis L., 87.9 mg GAE/ g), MP(Mentha piperascens Holmes., 85.1 mg), NN(Nelumbo nucifera Gaertn., 65.0 mg) and PD(Pinus densiflora Siebold & Zucc., 52.8 mg). Total flavonoids were high in NN(25.7 mg QUE / g) and MP (25.4 mg QUE / g). CS(58.1%), NN(47.9%), and MP(40.6%) showed high ABTS radical scavenging ability and the result was similar in DPPH radical scavenging ability. The extracts of HC(Hemerocallis coreana Nakai.), PD, and CO(Cornus officinalis Siebold et Zucc.) showed the highest inhibitory effect on the growth of E. coli. The extracts of PK(Pulsatilla koreana Nakai ex Nakai.), SC(Saururus chinensis Baill.), and MC(Smilax china L.) completely inhibited the proliferation of S. aureus, showing the possibility to be developed as natural preservatives and disinfectants.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.59-66
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• 2018

Propionibacterium acnes, a major pathogens bacterium of acne, is one of the microflora associated with the inflammation. In this study, the antibacterial effects of 70% ethanol extracts from the 61 natural plants in Jeju against P. acnes were investigated using the disc diffusion method. The 45 natural plant extracts showed antibacterial effects and the 16 natural plants extracts didn't show antibacterial effects. The Phyllanthus urinaria L. (stem and leaves) extract showed thr highest antimicrobial activity with $18.96{\pm}0.69mm$ clear zone of the growth of P. acnes, followed by Angelica gigas Nakai (roots), Gynostemma pentaphyllum (Thunb.) Makino, Angelica gigas Nakai (stem and leaves), Isodon inflexus (Thunb.) Kudo, camellia sinensis L. (flowers), Rosmarinus officinalis, etc. These results suggest that the natural plant in Jeju can be used as functional cosmetic biomaterials or antimicrobial agents against P. acnes.

• Korean Journal of Organic Agriculture
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• v.25 no.4
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• pp.901-916
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• 2017

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow ($450{\pm}30kg$) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall's buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.1-8
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• 2010

Occurrence of earthworms for turfgrass growing seasons and effect of earthworms by some plant extracts and plant oils in golf courses were investigated at the three locations golf courses in Korea 2005 to 2006. There were no differences in monthly occurrence of earthworm except Anseong Benest Golf Club, namely, Anseong Benest Golf Club was highly occurred in July in 2006. Density of earthworm was not different between fairway and rough in Dongrae Benest Golf Club (opened in 1971) while density was higher in rough than fairway in Anseong Benest Golf Club (opened in 1999) and Gapyeong Benest Golf Club (opened in 2000). In case of Gapyeong Benest Golf Club, earthworm density was higher in old courses than recently opened courses. Effect of plant extracts such as mustard oil, wintergreen oil, Quisqualis indica, Daphne genkwa, Pharbitis nil, Zingiber officinale, Xanthium strumarium, and Camellia sinensis on earthworm, mustard oil, wintergreen oil and tea saponin were highly toxic to earthworm, Eisenia andrei. In the pot experiment, 100% of Eisenia andrei was dead at the treatment of 500-fold of aquatic solution of tea saponin (12.4% a.i.).