Sampling of Earthworm Using Tea Tree (Camellia sinensis) Extract and Occurrence of Earthworm in Turfgrass of Golf Courses

차나무 추출물을 이용한 골프장의 지렁이 샘플링과 종류 조사

  • Ha, Jong-Won (Department of Applied Biology, Kyungpook National University) ;
  • Hong, Yong (Department of Agricultural Biology, Chonbuk National University) ;
  • Lee, Sang-Myeong (Korea Forestry Research Institute, Nambu Forestry Research Center) ;
  • Choo, Ho-Yul (Department of Applied Biology, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Kim, Jin-Ho (School of Ecological and Environmental System, Kyungpook National University) ;
  • Lee, Dong-Woon (Department of Applied Biology, Kyungpook National University)
  • 하종원 (경북대학교 생물응용학과) ;
  • 홍용 (전북대학교 농생물학과) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 추호렬 (3경상대학교 응용생물환경학과, 농업과학연구원) ;
  • 김진호 (경북대학교 생태환경시스템학부) ;
  • 이동운 (경북대학교 생물응용학과)
  • Received : 2010.11.08
  • Accepted : 2010.12.07
  • Published : 2010.12.31

Abstract

Effective sampling method and distribution of earthworms in turfgrasses of golf courses were investigated at 10 Korean golf clubs. Tea tree extract was highly effective in extracting Eisenia andrei compared with mustard oil or formalin. 1000-fold diluent of tea tree extract (4 L/$m^2$) was more effective than 500- and 2000-fold. Sampling efficacy of earthworm increased as soil depth decreased. Additional watering after treatment of tea tree extract did not significantly influence the sampling of earthworms. Eight earthworm species in 4 genera were collected from turfgrasses of 10 golf courses with 4 L of 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$. Among, dominant earthworm was Amynthas heteropodus and collected 147 individuals from 6 golf clubs. Thirteen Drawida japonica was collected from 3 golf clubs and A. hilgendorfi and A. hupeiensis were collected from 2 golf clubs. A. hupeiensis was not collected with 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$.

골프장 잔디에서 효과적으로 지렁이를 추출하는 방법과 골프장의 잔디에 서식하는 지렁이의 종류를 알아보기 위하여 연구를 수행한 결과, 차나무추출물이 mustard oil이나 formalin에 비하여 붉은줄지렁이(Eisenia andrei)의 추출에 효과가 있었다. 그리고 차나무추출물 1000배 처리가 (4 L/m2)가 500배와 2000배 처리에 비하여 효과가 높았다. 토양 내 서식 깊이가 얕을수록 추출효과가 높았다. 그러나 차나무추출물 처리 후 추가적인 관수는 추출에 크게 영향을 미치지 않았다. 우리나라 10개 골프장에는 4속 8종의 지렁이가 분포하고 있었다. 그 중 변이성지렁이(Amynthas heteropodus)가 6개 골프장에서 147개체가 채집되어 우점종이었으며 염주위지렁이(Drawida japonica)가 3개 골프장에서 13개체가 채집되었고, 외무늬지렁이(A. hilgendorfi)와 똥지렁이(A. hupeiensis)가 2개 골프장에서 채집되었다. 차나무추출물로는 똥지렁이가 탈출되지 않았다.

Keywords

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