• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.72-76
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• 2002

This study was carried out to determine an effective mechanical harvester for rhizomes of Rehmannia glutinosa Libosch. Labor-saving efficiency showed 69∼76% by using tillage operations with power tiller, digger attached to power tiller, and digger attached to tractor compared with manual harvest. Loss percentage of rhizomes by mechanical harvesters was 3.1∼ 9.3% higher, and fresh rhizome yield was 2∼6％ lower than that by manual harvest, respectively. Although it showed higher loss percentage of rhizomes and lower fresh rhizome yield compared with manual harvest, mechanical harvest using digger attached to power tiller or digger attached to tractor was effective in labor-saving for rhizomes of Rehmannia glutinosa Libosch.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.273-277
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• 2005

Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1 $mg/{\ell}\;BA\;and\;2\;mg/{\ell}$ IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1 $mg/{\ell}\;IBA$. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.273-276
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• 1998

Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the crude drug damand expands rapidly. This study was conducted to obtain the basic breeding information of Chinese foxglove. Meristem culture was employed for virus free seedling production and miropropagation. Both Jiwhang 1 and domestic local were severely infected by potexvirus and TMV. Several growth regulators were used for the virus free stock production from Jiwhang 1 and Danyang local. Shoot formation and callus induction from the meristem culture seemed to be influenced by the content of various kinds of plant growth regulators. Kinetin supplement was the most effective on shoot formation and NAA addition was good on callus induction among the treatments. The acquired virus free stocks were confirmed using transmission electron microscope and indicate plants.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.73-79
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• 2020

This study was conducted to evaluate the appropriateness cultivation of R. glutinosa by plug seedling. Cultivation by direct seeding (DS) and plug seedling (PS) of R. glutinosa was conducted in each of the Pyeongchang and Eumseong areas. As a result, locally, for the Eumseong area, aerial parts in leaf length, leaf width and number of leaves per plant were better than for the Pyeongchang area. In the cultivation method, leaf width and number of leaves per plant were better with PS cultivation than with DS cultivation. Locally, rhizome yield of underground parts in the Eumseong area was better than that in the Pyeongchang area, but the rhizome yield with DS cultivation in the Eumseong area and PS cultivation in the Pyeongchang area were statistically the same. The fresh rhizome yield for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 1,253 kg/10a, 1,376 kg/10a, 923 kg/10a and 1,256 kg/10a, respectively. Finally, the catalpol content for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 3.67%, 2.03%, 2.96% and 1.60%, respectively. From these results, it can be seen that the R. glutinosa PS cultivation method can increase the rhizome yield.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.55-60
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• 2004

This experiment was carried out to develop rapid mass propagation via shoot organogenesis system from adventitious roots of Rehmannia glutinosa. The induction of adventitious roots from leaf explants was most favorable to MS solid medium supplemented with 2mg/L IBA. However, the growth of adventitious roots was highest when they were cultured on 1/3 strength MS liquid medium supplemented with 2mg/L IBA. When the adventitious roots were grown in 10L bioreactor, 10g roots as initial inoculum was increased to 225g after 6 weeks of culture. The harvested roots were cultured onto solid medium to induce plant regeneration. The optimal adventitious shoot formation was observed on MS medium supplemented with 2mg/L BA. Rooting of individual shoots was induced after transfer to half strength MS medium without growth regulators. Plantlets after acclimatization were successfully transplanted in the field and no phenotypic variation was observed among them.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.120-124
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• 1995

This experiment was carried out to elucidate the effect of planting depthon the quality and yield in Rehmannia glutinosa . Planting depths were 3, 6, 9 and 12cm, and planted in April 18. Emergence rate were decreased in the order of 86 % in 3cm, 67 % in 6cm, 37 % in 9cm and 21 % in 12cm. Leaf length was longer about 0.3 - 0.8cm in planting depth $6{\sim}12cm$ than that of 15.6cmin planting depth 3cm. Yield of Rehmannia glutinosa were lower 5% in plantingdepth 6cm, 62% in 9cm and 68% in 12cm than that of 1,154kg/10a in planting depth 3cm. And optimum planting depth were considered 6cm.

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.35-42
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• 2018

This study was carried out to investigate site and soil factors affecting growth of root collar diameter (RCD) and height (HT) of Larix kaempferi trees planted in harvested Larix kaempferi plantations. We were found effect factors of growth of RCD and HT using 15 of site and soil factors. And then we made the growth estimated equations of RCD and HT of 6-years-old L. kaempferi tree by the derived affecting factors. The growth of RCD was affected in order of available phosphorus (+), effective soil depth (+), organic matter (-), sand separate in soil (-), pH (-), and elevation (-), and the coefficient of determination ($R^2$) of the growth estimated equation of RCD was 0.51. The growth of HT was affected in order of effective soil depth (+), elevation (-) slope (-), and available phosphorus (+) and the $R^2$ of the growth estimated equation of HT was 0.46. The growth of RCD and HT were commonly affected by effective soil depth, available phosphorus, and elevation. Therefore, planting site of L. kaempferi should be considered the mainly effect factors in order to keep better early growth based on the current study.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.140-144
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• 1997

This experiment was carried out to investigate the proper planting method of Rehmannia glutinasa Liboschitz. A Chungbuk local cultivar was sown on the seed bed of 200cm with the planting density of 30cm(6 rows) $\times$ 8.5cm(30 rhizome per $\textrm{m}^2$). The experimental design was randomized block design with 3 replications. And the result was summarized as follows. The emergence date of level Planting(control : 0$^{\circ}$) was May 23, but the emergence dates of Oblique planting(45$^{\circ}$) and Upright planting(90$^{\circ}$) treatments were later than the control by 2~4 days. And the leaf length and the ratio of leaf length/leaf width were not significantly different. As compared with the 29.2cm and 10.0mm of the rhizome length and the rhizome diameter of level planting treatment, the rhizome length of Oblique planting treatment was decreased by 3.4cm and 0 mm. The length and diameter of rhizome of the Upright planting treatment was decreased by 2.8cm and 1.0mm. And the order of sugar content was level Planting > Oblique planting > Upright planting. The total yields were decreased by 9% in the Oblique planting and by 15% in the Upright planting compared to 1,203kg/10a in the control, and the commercial yields showed the same tendency. The level planting treatment was found to be most proper for sowing of Rehmannia glutinasa Liboschitz in the middle inland region of Korea.

• Korean Journal of Plant Resources
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• v.24 no.1
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• pp.105-112
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• 2011

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of thirteen kinds of natural plant pigments. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging and nitrite scavenging ability activity. The free radical scavenging activity by method using stable free radical DPPH was the highest in the red cabbaged pigment. Addition of ethanol extract 1mg/ml from onion peel pigment displayed remarkable effect on nitrite scavenging ability about 91.9%. Antioxidative enzyme activity was evaluated in terms of superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX) activity. The bitter melon pigment had the highest SOD activity of 87.3%. The activities of CAT and APX were higher in the mulberry leave pigment compared with other natural plant pigments. In contrast, CAT activity of plant pigment samples were unaffected. These results suggest that natural plant pigment had the potent biological activities such as antioxidant enzyme activities, and that their activities exhibited differently depending on each kind of pigments.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.12-15
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• 1995

This experiment was carried out to elucidate the effect of planting depth on the Quality and yield in Rehmannia glutinosa. Planting depths were 3,6,9 and 12cm, and planted on April 18. Emergence rate was decreased in the order of 86% in 3cm, 67% in 6cm, 37% in 9cm and 21% in 12cm. Leaf length was longer about $0.3{\sim}0.8cm$ in planting depth 6-12cm than that of 15.6cm in planting depth 3cm. Yield of Rehmannia glutinosa were lower 5% in planting depth 6cm, 62% in 9cm and 68% in 12cm than that of l,154kg/10a in planting depth 3cm. And optimum planting depth was considered 6cm.