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Flower and Microspore Development in 'Campbell Early' (Vitis labruscana) and 'Tamnara' (V. spp.) Grapes ('캠벨얼리'와 '탐나라' 포도의 꽃과 소포자 발달)

  • Yim, Bomi;Mun, Jeong-Hwan;Jeong, Young-Min;Hur, Youn Young;Yu, Hee-Ju
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.420-428
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    • 2015
  • The majority of cultivated varieties of grape have perfect flowers that are clustered in an individual inflorescence. Grape flower has a single pistil, five stamens, a protective flower cap (calyptra), and a calyx. After fertilization, an individual flower develops into a single berry. Although there are a number of reported studies focusing on berry formation, berry enlargement, and sugar accumulation in grape, the morphological studies of flower, including gametophyte morphogenesis and structural change in floral organs, have not yet been studied in detail. In this study, we investigated the flower structure and development characteristics of grape using microscopy and defined the floral development stages 9 to 13 based on microspore or male gametophyte development stage from tetrad to mature pollen. We used seeded diploid table grapes 'Campbell Early' (Vitis labruscana) and 'Tamnara' (V. spp.) as plant materials. At floral development stage 9, pollen mother cells develop to tetrads. During floral development stages 10 to 11, unicellular microspore develop to mid bicellular pollen. At the end of floral stage 12, male gametophyte develops to mature tricelluar pollen. In floral stage 13, the flower cap falls off and flower bud opens. During floral development stages 9 to 12, there were no major changes in calyx length, whereas the length of the flower cap continuously increased. The flower cap-to-calyx length ratio was 2.0, 3.0, 4.5, and 6.5 at floral stages 9, 10, 11, and 12, respectively. The flower cap-to-calyx length ratio was consistent in the two grape cultivars, suggesting that the ratio is a morphological character representing floral development stage. This study provides a reference for determining floral development stage of the two grape cultivars. It will be useful for the determination of optimum time for microspore culture needed to generate doubled haploid lines and appropriate gibberellic acid treatment needed to induce parthenocarpic fruit development in 'Tamnara' grape.

Evaluation of Environment-friendly Control Agents for the Management of Powdery Mildew Infection during Seedling Stage of Three Cucurbitaceae Vegetables (친환경 육묘시 세 가지 박과채소의 흰가루병에 대한 친환경 제제의 방제효과)

  • Yeo, Kyung-Hwan;Jang, Yoon Ah;Kim, Su;Um, Young Chul;Lee, Sang Gyu;Rhee, Han Cheol
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.413-420
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    • 2013
  • The purpose of this study was to evaluate the control efficacy of major environment-friendly control agents against powdery mildew, as affected by the application condition such as disease-developing stage and microclimate, as compared with the efficacy of pesticides in plug seedling of three Cucurbitaceae vegetables, including cucumber, melon, and oriental melon. Single or combined application of major six environment-friendly control agents was used in the experiment: two biofungicide (Ampelomyces quisqualis 94013 and Bacillus subtilis Y1336), two plant extracts (neem oil and extracts of Rheum undulatum), and two mineral materials (wettable sulfur powder and lime bordeaux mixture). These control agents were treated to the plug seedlings for preventing powdery mildew and curative applications for managing powdery mildew. In all treatments, the disease incidence declined as daily average temperatures increased to $30^{\circ}C$ for consecutive 6~8 days with maximum temperature over $40^{\circ}C$. In preventative application, the control efficacy against powdery mildew was the highest in the treatment of wettable sulfur powder, and lowest in the B. subtilis Y1336, with values of 20~40%. In cucumber seedlings, the preventive single application of neem oil or wettable sulfur powder was more effective than curative application of fungicides, while the control efficacy of these agents was similar to those of fungicides in melon seedlings. The single application of R. undulatum extracts was also effective in preventing the disease for both cucumber and melon seedlings, showing a higher control efficacy than those of biofungicides during seedling stage. The treatment of water spray was not effective and showed a higher disease incidence than the untreated control plot in the oriental melon and melon seedlings. The curative application with environment friendly control agents, after powdery mildew was first detected, could not successfully controlled the disease at the middle stage (5~10% of disease incidence) of disease development. The curative combined application of [R. undulatum extracts (1st application) + wettable sulfur powder (2nd) + neem oil (3rd)] showed the highest control efficacy among the other treatments, with control value over 80% at the early stage (less than 1% of disease incidence) of disease development.

Clinical Analysis of Infective Endocarditis (감염성 심내막염의 임상적 고찰)

  • Kim, Hyuck;Kim, Young-Hak;Chung, Won-Sang;Shin, Kyung-Wook;Kim, Ji-Hoon
    • Journal of Chest Surgery
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    • v.43 no.6
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    • pp.619-626
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    • 2010
  • Background: The indications and the optimal time of surgery of infective endocarditis are controversial. We report the surgical results of our hospital during the last 10 years with literature review. Material and Method: Between January 2000 and December 2009, we enrolled 23 infective endocarditis patients who underwent surgery, and analyzed retrospectively. In the preoperative blood culture, 8 cases (34.8%) were positive. The average preoperative antibiotics treatment period was $20.78{\pm}16.00$ days. There were 12 (52.2%) urgent operations. The average follow up period was $49.26{\pm}33.21$ months. Result: 20 mechanical valve replacements were performed, 9 in aortic position, 8 in mitral position and 3 in the both positions. The other procedures were one mitral valvuloplasty, one infected myxoma extirpation, and one infected pacemaker lead removal with debridement. The average period of postoperative intravenous antibiotic treatment was $24.39{\pm}15.98$ days. There were 5 complications, including 2 cases of postoperative bleeding, one postcardiotomy syndrome, one cerebral ischemia, and a low cardiac output syndrome. There were statistically significant postoperative improvement in NYHA class, left ventricle end diastolic/end systolic volume, and left atrium size (p-value < 0.05). Conclusion: We could obtain the satisfactory results without any moftalities by using sufficient preoperative antibiotics in hemodynamically stable patients, and by prompt surgery in unstable patients.

The Cultural Landscapes of Wuyi-Gugok of China as seen from the 「Landscape of the Jiuqu River in the Wuyi Mountain」 in British Library (대영도서관 소장 「무이산구곡계전도(武夷山九曲溪全圖)」로 본 중국 무이구곡의 문화경관상)

  • Cheng, Zhao-Xia;Rho, Jae-Hyun;Jiang, Cheng
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.37 no.4
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    • pp.11-31
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    • 2019
  • Taking the painting, 「Landscape of the Jiuqu River in the Wuyi Mountain」 as the study object, which was produced in the middle of Qing Dinasty and collected by the British Library, this paper analyzes the scenery names recorded in the painting, and describes the landscape of the mountain, port and ships, architectural elements, civil elements, character, stone inscription and other scenery in the painting. The investigation results of the cultural landscape properties of each Gok are as follows: According to statistics, there are 28 architectural elements in the painting, including 7 pavilions (25%), 4 temples (14.3%), 3 Colleges and Taoist temple (10.7%), 2 Dowon(道院) and villages (7.1%); 29 civil elements, including 9 holes (31%), 6 Historical Sites (20.7%), 3 Stations(臺) (10.3%), 2 Ferries, 2 Bridges, and 2 Ponds (6.9%), 1 Garden, 1 Gate, 1 Mine(坑), 1 Well and 1 Remains(3.4%). These physical factors and civil factors are the important relics reflected the cultural landscape attributes of Wuyi-Gugok in the middle of the 18th century. Among the shape element in each Gok, the 1st Gok have 12 shape elements(21.1%), the 5th Gok 11(19.3%), the 4th Gok 9(15.8%), the 9th Gok 8(14%), the 3rd Gok 7(12.3%), the 6th Gok 4(7%), the 2nd Gok 3(5.3%), the 7th Gok 2(3.5%), and the 8th Gok 1(2%). Through collation, it is found that the 1st Gok, 5th Gok and 4th Gok have more prominent cultural landscape characteristics. In addition, according to the description of scenic spot types in 『Muisanji(武夷山志)』, there are 38 types of scenery description in the painting, of which, the three scenery of big rock, peak, small rock occupy the vast majority. This reflects the Danxia(丹霞) landform characteristics of Wuyi-Gugok. The cultural connotation of Wuyi Mountain expressed and contained in the painting is analyzed and interpreted, and it is found that the Jiuqu(九曲) River in the Wuyi Mountain has Neo-confucianism culture, Taoism culture, Buddhism culture, Tea culture and so on. In addition, among the 171 scenery names shown in the painting, there are altogether 7 stone inscriptions that are consistent with or have the same meaning as the rock inscriptions site, including 3 for inscriptions praising the landscape, 3 for philosophical inscription and 1 for auspicious language inscription, which is considered as the important basis for the mutual textuality between the pictures and the stone inscriptions.

A Comparative Study of the Foreign Trade Strategies of Gaisong Merchants and Modern Companies in Korea. (현대기업과 개성상인의 해외진출전략의 비교분석)

  • Park, Sang-Gyu
    • Korean Business Review
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    • v.17
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    • pp.153-183
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    • 2004
  • The Gaisong Merchants can be regarded to playa pioneering role to activate the Korea's trade with foreign countries. In the early period of Yi-Dynasty, the Gaisong Merchants focused on personal trade, but in the middle period of Yi-Dynasty, they entered to the realm of governmental trade. Furthermore, their business activities widened to various forms of trades, for example, smuggling. Utilizing accumulated capital, Gaisong merchants expanded their trading activities to their neighboring countries such as Japan and China. In recent times, it is necessary for modem Korean companies to diversify risks through the establishment of corporations for production, marketing and R&D abroad or through joint venture, M&A and strategic alliance with foreign companies in order to reduce the risks originated from volatile economic and political situations. In this study, we utilize tools of comparative study to compare Gaisong Merchants' foreign trade strategies with those of modem companies such as AMOREPACIFIC, HANILCEMENT and SHINDORICO. The purpose of the paper is to test the hypothesis that modem Korean companies grew up by following the cases of Gaisong Merchants' business activities. We summarize our main findings as follows. First, both Gaisong Merchants and modem Korean companies have common functional core capability in the field of marketing, manufacturing technology, R&D, and human resources development. Second, both Gaisong Merchants and modem Korean companies have common organizational core capability. Third, both Gaisong Merchants and modem Korean companies have common infrastructures such as planning, finance, accounting and MIS. It constitutes the infrastructure of Korea's foreign trade sector. Fourth, both Gaisong Merchants and modem companies have common organizational culture in the field of management policy and philosophy. Actually, those factors are evaluated to be driving forces of Koera's success in foreign trade. In conclusion, the business activities of Gaisong Merchants who represented the peculiarity of Korean business spirit are partially inherited to current Korean business management. The value system and behavior pattern of modern Korean companies is succeeded from the spirit of Gaisong Merchants and it playa major role to specify the identity of Korean business administration.

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Identification of bacteria from the peri-implant sulcus of orthodontic mini-implants using 16S rDNA clone library (16S rDNA 클론 library 제작 및 핵산염기서열 결정을 통한 교정용 미니임플랜트 주위 열구의 세균 동정)

  • Lim, Sung-Hoon;Kim, Kwang-Won;Yoo, So-Young;Kook, Joong-Ki;Chang, Young-Il
    • The korean journal of orthodontics
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    • v.36 no.4
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    • pp.251-262
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    • 2006
  • Objective: The purpose of this study was to compare the bacterial flora at the peri-implant sulcus of the orthodontic mini-implant placed in the alveolar mucosa with the bacterial flora at the adjacent healthy gingival sulcus. Methods: Two plaque samples from 7 patients were collected by inserting paper points into the sulcus between the mini-implant and ligature wire connected to the mini-implant head and inflamed alveolar mucosa, and from the gingival sulcus of a healthy tooth adjacent to the mini-implant. Results: Using 16S rDNA clone library, the 24 kinds of bacteria including Haemophilus aphrophilus, Sphingomonas species, Capnocytophaga species, Prevotella melaninogenica, Lachnospiraceae species, Porphyromonas species, Neisseria flava were identified only from the sulcus around the mini-implant. These bacteria constituted only 9.2% of total clones, and the bacteria identified from both the sulcus around mini-implants and the gingival sulcus constituted 80.4% of total clones. Of these bacteria, clones of Prevotella species, Atopobium rimae, Veillonella species, Streptococcus intermedius/constellatus, Streptococcus salivarius were more frequently isolated from the peri-implant sulcus. Conclusion: This study suggests that a broad epidemiological study is needed to find causative bacteria which induce inflammation from the peri-implant sulcus.

Study on Sacrifice Food of Bulchunwi Sacrificial Ceremony in Sangju Area (상주지역의 불천위제사 제수문화에 관한 연구)

  • Kim, Gwi-Young;Kim, Bo-Ram;Park, Mo-Ra
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.5
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    • pp.739-751
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    • 2015
  • This study investigated the table settings used in Bul-chun-wi sacrificial rituals in Sangju, Gyeongpook Province. This study took place from May 2014 to April 2015 and incorporated field research and literature review. The subjects of the study were Sojae jongga (family head), Wolgan jongga, Ubook jongga and Ipjae jongga. There were four rows in the table setting of the Wolgan, whereas there were five rows in the other jonggas. In row 5, there were fruits, seeds and confections. In row 4, there were vegetables. In row 3, there was tang (stew); in row 2, juk (steamed meats or fishes) and pyun (rice cake); and in row 1, meh (boiled rice), geng (soup) and myun (noodles). The common food setting was arranged in the order of meh, geng, myun, ganjang (Korean soy sauce), poh (dried meat/fish), chimchae (water kimchi), dates, chestnuts, pears, persimmons, walnuts, bracken, bellflowers, croaker, pyun, and ssam (rice wrapped in lettuce leaves). Ubook jongga had the most number of stews at seven. Sojae and Ipjae jongga each had five stews, and Wolgan jongga had three. The smaller Wolgan jongga had gehjuk (boiled chicken), uhjuk (steamed fish) and yookjuk (boiled meat) arranged in a neat pile on one dish used for rituals, whereas Ubook jongga had gehjuk, uhjuk and yookjuk arranged separately. Sojae and Ipjae jongga had gehjuk arranged separately while yookjuk and uhjuk were arranged as dojuk (gehjuk, uhjuk, yookjuk). The uhjuk was arranged separately. In Wolgan jongga, po was arranged on the right while in the other jonggas, it was arranged to the left. For raw meat Ubook jongga, raw mackerel; Ipjae jongga, raw croaker, and Sojae jongga Yukhoe (beef tartare) were set. However, pork, chicken and croaker were cooked. Other ritual food were used in seasoning and boiling. In the geng Wolgan and Ipjae jongga used miyeokguk (seawood soup), whereas Sojae and Ubook jongga used tangguk (meat soup with vegetables). For the ritual food, most jongga ordered yoogwa, yakgwa and bonpyun as one part, rice cakes (steamed rice cake, glutinous rice cake, jeungpyun, gyungdan), and jehju (alcohol). The other ritual food was prepared on the day by jongga.

Effect of Temperature and Food Source on the Egg and Larval Development of Tobacco Cutworm, Spodoptera litus Fabricius (온도 및 기주조건이 담배거세미나방(Spodoptera litura)의 난 및 유충발육에 미치는 영향)

  • 배순도;박경배;오윤진
    • Korean journal of applied entomology
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    • v.36 no.1
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    • pp.48-54
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    • 1997
  • This study was conducted to determine the effect of temperatures and food sources on the egg and larval developmentof the tobacco cutworm, Spodoptera litura Fabricius. The hatchability of egg masses of S. liturawas 100% on the leaf of soybean, perilla and sweet potato in any given temperature regimes, while the hatchabilitywas only 65-8796 when reared on the pulp paper and decreased as temperature increased. Egg durationwas not significantly different among different food sources within each temperature. However, egg duration at32$^{\circ}$C was shorter than that at 24$^{\circ}$C and 28$^{\circ}$C. During the early larval development, at 28$^{\circ}$C and 32$^{\circ}$C the larvafed on sweet potato leaf was heavier than those fed on soybean and perilla leaves and the opposite case wastrue during mid-larval development stage. However, larval weight at 24$^{\circ}$C was heavier on sweet potato leafthan that on soybean and perilla leaves until 12 days after hatching. This result was probably due to relativelyslower developmental rate at 24$^{\circ}$C compared to 28$^{\circ}$C and 32$^{\circ}$C. The mean larval mortality was 68.896, 44.5%and 33.8% at 24$^{\circ}$C. 28$^{\circ}$C and 32"C, respectively. The lowest mortality was observed on soybena leaf and followedby perilla and sweet potato leaves, and artificial diet regardless of temperature conditions. The durationwas the shortest when they fed on soybean leaf, and followed by perilla and sweet potato leaves and artificialdiet. Larval durations were 23.6-30.4 days at 24$^{\circ}$C. 18.6-22.3 days at 28$^{\circ}$C and 14.5-18.0 days at 32$^{\circ}$C. Thethreshold temperatures of egg and larva of S. litura were about 6.l"C and 10.9"C, respectively.t 6.l"C and 10.9"C, respectively.pectively.

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OBSTETRICIAN'S VIEW OF TEENAGE PREGNANCY:PRESENT STATUS, PREVENTION AND PSYCHIATRIC CONSULTATION (산과 의사가 인지한 10대 임신의 현황, 예방, 정신과 자문)

  • Kim, Eun-Young;Kim, Boong-Nyun;Hong, Kang-E;Lee, Young-Sik
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.13 no.1
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    • pp.117-128
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    • 2002
  • Objectives:For the purpose of obtaining the more vivid present status and prevention program of teenage pregnancy, this survey was done by Obstetricians, as study subject, who manage the pregnant teenager in real clinical situation. Methods:Structured survey form about teenage pregnancy was sent to 2,800 obstetricians. That form contained frequency, characteristics, decision making processes, and psychiatric aspects of the teenage pregnancy. 349 obstetricians replied that survey form and we analysed these datas. Results:(1) The trend of teenage pregnancy was mildly increased. (2) The most common cases were unwanted pregnancy by continuing sexual relationship with boyfriends rather than by forced, accidental sexual relationship with multiple partners. (3) The most common reason of labor was loss the time of artificial abotion. (4) Problems of pregnant girls' were conduct behaviors and poor informations about contraception rather than sexual abuse or mental retardation. (5) Most obstetricians percepted the necessity of psychiatric consultation, however psychiatric consultation was rare due to parents refusal and abscense of available psychiatric facility. (6) For the prevention of teenage pregnancy, the most important thing was practical education about contraception. Conclusions:Based on the result of this study, further study using structured interview schedule with pregnant girl is needed for the detecting risk factor of teenage pregnancy and effective systematic approach to pregnant girl.

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Effects of Paddy-Upland Rotation Systems on Nutrient Balance and Distribution in Soil Profile (답전윤환(畓田輪換) 체계(體系)에 따른 토양(土壤)의 층위별(層位別) 양분분포(養分分布) 및 양분수지(養分收支))

  • Ahn, Sang-Bae;Motomatsu, T.;Lee, Sang-Eun
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.2
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    • pp.98-104
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    • 1994
  • Effects of paddy-upland rotation system on nutrients distribution in soil profile and nutrient balance were studied in paddy fields from 1989 to 1993. The obtained results are summarized as follows. 1. $NO_3{^-}-N$, Av.$P_2O_5$, Ex.-K, and EC were high by small extent in surface layer of 0~20cm soil depth without the sign of salt movement to deeper layers. On the contrary Ex.-ca, Ex.-Mg, and pH became high with increase of soil depths. 2. $NO_3{^-}-N$, Av.$P_2O_5$, Ex.-K, and EC in surface soil were high in the order of Converted, Paddy-Upland Rotation, Potato-Chinese Cabbage>2 Year, Rotation, Potato-Chinese Cabbage>Converted, Paddy-Upland Rotation, Soybean>Continous Paddy, which responded well to fertilizer application rates. On the other hand Ex.-Ca, Ex.-Mg, and pH in whole layers were high in the order of Converted, Paddy-Upland Rotation, Soybean>Converted, Paddy-Upland Rptation, Potato-Chinese Cabbage>2 Year, Rotation, Potato-Chinese Cabbage>Continuous Paddy, which largely depended on plant absorption. 3. Nutrient balance in upland cropping system cultivating potato and Chinese cabbage showed that the input of chemical fertilizer of nitrogen and potassium was less than the plant uptake, while it was reverse for phosphorus with much gap between fertilizer input and plant uptake. Therefore, phosporous was expected to be accumulated by 27kg/10a every year. 4. Nutrient balance in upland cropping system cultivating soybean showed that nitrogen was not deficient to soybean crops even the chemical fertilizer input was less than plant uptake because of nitrogen fixation by rhizobia. However, there was about 1kg/10a deficit of potassium, which resulted dificiency symptom in the middle stage of soybean growth. For phosphorous there was excess of 4kg/10a, which was expected to be short for maintaining phosphorous fertility of upland soils.

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