• Journal of Bio-Environment Control
• /
• v.21 no.1
• /
• pp.45-49
• /
• 2012

Chilean wild flower seeds showed low germination rate and lacked germination uniformity. This experiment was conducted to find out the effects of light conditions, $GA_3$ (50 mg/L, 100 mg/L, 200 mg/L) and $KNO_3$ (0.1%, 0.2%, 0.4%) treatments on germination of five species of Chilean wild flower seeds. The seeds of $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$, and $Schizanthus$ $litoralis$ did not germinated in light condition. The others did not show a significant different germination rate under light and dark conditions. The germination rate of $Mimulus$ $luteus$, $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$ increased 2.3, 5.6, and 3.2 times compared with control, respectively in $GA_3$-200 mg/L treatment, but that of $Rhodophiala$ $ananuca$ enhanced slightly in $GA_3$-50 mg/L treatment. Those results suggested that the germination rate of Chilean wild flower seeds enhanced by $GA_3$ treatment that is very efficient in breaking seed dormancy.

• Journal of Bio-Environment Control
• /
• v.16 no.1
• /
• pp.32-39
• /
• 2007

This study was carried out to find the optimum treatment condition for the improving germination using the plant growth regulators with $GA_3$ and BAP, to Kentucky bluegrass and Tall fescue showing low germination. $GA_3$ treatment was more effective on the germination of Kentucky bluegrass than BAP single treatment or the combination treatment of $GA_3$ and BAP, regardless of their treatment concentration. Seed germinability was 6 days treatment was significantly better than that of 3 days treatment in the experiment for treatment period of the plant growth regulators. When seeds were treated with $1000{\mu}M$ of $GA_3$ for 6 days, on the percent germination was improved as much as between 10% and 38% comparing with the control. In Tall fescue seed, the percent germination was not significantly improved, although it was slightly increased when the plant growth regulators were treated. For the optimum condition, on percent germination was improved as much as 10% comparing with the control of Tall fescue treated with $1000{\mu}M$ of $GA_3$ for 3 days. Percent emergence fur the seeds of Kentucky bluegrass and Tall fescue treated $GA_3$ in the field was improved as much as $8{\sim}9%$ comparing with the control, and mean emergence time $(E_{50})$ was also faster for $1{\sim}2$ days than that of the control. However, dry weight, plant height and root length showed no significant effects, although those were slightly improved more than those of the control.

• Korean Journal of Agricultural Science
• /
• v.11 no.2
• /
• pp.201-206
• /
• 1984

In order to obtain an easy and effective scarification method for stimulating the seed germination of Korean lawn grass (Zoysia japonica Steud.), sodium hypochlorite (SH), which is used as a commercial bleach and a seed disinfestant, was tested as a chemical scarifier instead of potassium hydroxide (KOH). 1. Scarification with 4% SH for 2 hours followed by 8-hour water-rinse stimulated seed germination more effectively compared to generalized scarification method by KOH treatment. 2. The degree of stimulating seed gemination was different by the various water-rinsing time after SH treatment, but the optimum rinsing time was about 4 to 8 hours. 3. GA treatment after SH scarification stimulated seed germination. However, the stimulation effect of GA was not showed in non-scarified control. 4. SH treatment significantly decreased the percentage of the infection of microflora in seed samples compared to KOH treatment. 5. Under practical seed scarification conditions for stimulating germination of Korean lawn grass seed. SH may have advantages over KOH with respect to ease of preparing, securing and handling.

• Korean Journal of Soil Science and Fertilizer
• /
• v.25 no.2
• /
• pp.160-167
• /
• 1992

The growth of weeds and some other plants has been considered to be inhibited by sicklepod(Cassia tora L.) sharing the habitat. The study was conducted, for the first time, to propose that this phenomenon is not only due to competition for physical and nutritional conditions but also due to allelopathy. In addition, autotoxicity of sicklepod was examined. The results are summarized as follows. 1. Rice sheath length reduced progressively from 18 % to 36 % with increasing the concentration of treating aqueous extracts of sickle pod seeds, but rice germination was not affected. In contrast, radish hypocotyl length was not reduced by the aqueous extract treatment but radish germination was significantly reduced by 66 % at 1 : 10 and 1 : 5 treatment. 2. Total chlorophyll contents in rice seedling decreased from 50 % to 65 % by treatment of seed aqueous extracts diluted from 1 : 50 to 1 : 5 ratio. 3. Aqueous extracts of sicklepod leaves significantly reduced hypocotyl length and fresh weight in radish and germination in rice, but mung bean was slightly affected by aqueous extracts only in fresh weight. 4. Volatiles from fresh, immature seeds with husk reduced the radish germination and seedling growth and radish root growth appeared to be more sensitive to the exposure to volatiles from fresh immature seeds than both germination and hypocotyl growth. 5. Volatiles from sickepod leaves inhibited germination and growth of radish, rice and mung bean, and seedling growth was more sensitive to volatiles from leaves than germination. 6. Volatiles from sicklepod leaves reduced germination and radicle length of sicklepod itself. 7. Collectively, it is concluded that there are water-soluble and volatile substances responsible for allelopathy in sicklepod.

• Korean Journal of Weed Science
• /
• v.8 no.3
• /
• pp.265-272
• /
• 1988

This experiment was carried to investigate the germination pattern in relation to temperatures and lights, and the emergence pattern in relation to seeding depths, lights and the alpha amylase activity of Youngia sonchifolia, Lactuca indica var. laciniata, Ixeris dentata var. albiflora and Ixeris polycephala. In Y. sonchifolia, the optimum germination temperature was $25^{\circ}C$, the optimum seeding depth to emerge was 0 mm and it could emerge in 0-5mm. In L. indica var. laciniata under cool storage, the optimum germination temperatures were $19^{\circ}C-28^{\circ}C$, the optimum seeding depth was 5mm and it could emerge in 0-20mm. In L. indica var. laciniata under room storage, the optimum germination temperature was $25^{\circ}C$ the optimum seeding depth was 5mm and it could emerge in 0-10mm. In I. dentata emerge was and 0mm and it could emerge in 0-5mm. In I. polycephla, the optimum temperatures were $16^{\circ}C-19^{\circ}C$, the optimum seeding depth to emerge was 0mm and it could emerge in 0-5mm. The alpha amylase activity was lower Y. sonchifolia, L. indica var. laciniata and I. dentata var. abiflora than barley cultivar Dongbor#1. And the increased pattern of alpha amylase activity was likely to it of germination rate.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.1
• /
• pp.81-85
• /
• 1989

One of the most important cultural techniques of pearl millet (Pennisetum americanum (L.) Leeke) is to encourage rapid and uniform emergence of seedlings to establish good stand and to let them grow well. Thus the objectives of the study were to investigate the effects of pre-sowing seed soaking and planting depth on dormancy breaking, germination and emergence of the seedlings, and to estimate the optimum planting season of pearl millet in Suwon, Korea. The seeds with dormancy germinated 99 to 100 percent when soaked in the H$_2$O$_2$ 1％ solution for 24 hours and rinsed with pure water, but germinated only 38％ and 83％ when soaked in pure water for 24 hours just after harvest and drying, and one month later from the harvest time, respectively. The seeds of Australia inbred line did not germinate at the constant 10$^{\circ}C$, but germinated at the constant 11$^{\circ}C$. It also was possible to estimate the optimum planting season by applying minimum temperature 11$^{\circ}C$ for germination. The minimum air temperature reached from late April in Suwon, Korea in regular years but fluctuated from late April to early May in 1986 and 1987. Thus, the safe planting season was mid-May for rapid and uniform germination of pearl millet seed. The optimum depth of planting was 2∼4cm under the optimum soil moisture condition, and 4 to 6 cm under the drier soil moisture condition. Subcoleoptile internode(mesocotyle) length increased according to increased depth of planting. Seedling crown placement also became deeper due to deeper planting of the seeds. The subcoleoptile internode length and seedling crown depth were positively correlated with actual planting depth, indicating that deeper planting would be not good for appropriate adventitious root and tiller development.

• Korean Journal of Plant Resources
• /
• v.25 no.1
• /
• pp.14-23
• /
• 2012

This study was performed to investigate the effects of storage, temperature, and pre-treatments on the germination of Melia azedarach seeds collected from Buan, Jeonju, and Jeju provenance. M. azedarach seeds stored with or without pericarp in the ground, which collected from Buan provenance evidenced the highest germination percentage (PG, %) and the shortest time to first germination (days). The seeds collected from Jeonju and Jeju provenance were placed at both six continuous temperatures (15, 20, 25, 30, 35, and $40^{\circ}C$) and two alternating temperatures ($20{\leftrightarrow}30^{\circ}C$ and $25{\leftrightarrow}35^{\circ}C$) for seed incubation. The results showed a significant effect for temperature of seed incubation. The seeds incubated at $35^{\circ}C$ had the highest PG among the continuous temperatures and germinated significantly more at the two alternating temperatures than at $35^{\circ}C$. Concerning mean germination time (MGT), the seeds incubated at $35^{\circ}C$ evidenced the shortest germinations among the continuous temperatures while those at the alternating temperatures germinated for a shorter period than those at $35^{\circ}C$. The germination rate (GR) and germination performance index (GPI) were similar to PG. The seeds collected from Jeonju provenance were treated using five pre-sowing treatments (scarification, scarifcation+$GA_3$, scarification+$KNO_3$, $GA_3$, and $KNO_3$) prior to the germination experiments. Compared with the intact seeds (control), most of the pre-treatments were significantly (especially scarification+$GA_3$ 100 ppm and scarification+$KNO_3$ 1.0%) higher in PG, GR, and GPI, as well as shorter in MGT.

• Journal of Nutrition and Health
• /
• v.54 no.6
• /
• pp.603-617
• /
• 2021

Purpose: This study was carried out to investigate the effect of amaranth seed extracts on glycemic regulation in HepG2 cells. The 80% ethanol extracts of amaranth seeds were used to evaluate α-amylase and α-glucosidase activities, cell viability, glucose uptake and messenger RNA (mRNA) expression levels of acetyl-CoA carboxylase (ACC), glucose transporter (GLUT)-2, GLUT-4, insulin receptor substrate (IRS)-1 and IRS-2. Methods: The samples were prepared and divided into 4 groups, including germinated black amaranth (GBA), black amaranth (BA), germinated yellow amaranth (GYA) and yellow amaranth (YA). Glucose hydrolytic enzyme, α-amylase and α-glucosidase activities were examined using a proper protocol. In addition, cell viability was measured by MTT assay. Glucose uptake in cells was measured using an assay kit. The mRNA expression levels of ACC, GLUT-2, GLUT-4, IRS-1 and IRS-2 were measured by reverse transcription polymerase chain reaction. Results: The inhibitory activities of α-amylase and α-glucosidase were highly observed in GBA, followed by BA, GYA and YA. Similar results were observed for glucose. The GBA effect was similar compared to the positive control group. The mRNA expression levels of ACC, GLUT-2, GLUT-4, IRS-1, and IRS-2 were significantly increased. The potential hypoglycemic effects of amaranth seed extracts were observed due to the increase in glucose metabolic enzyme activity, and glucose uptake was mediated through the upregulation of ACC, GLUT-2, GLUT-4, IRS-1, and IRS-2 expression levels. Conclusion: Our findings suggest that the amaranth seed is a potential candidate to prevent a diabetes. The present study demonstrated the possibility of using amaranth seeds, especially GBA and BA for glycemic control.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.3
• /
• pp.341-349
• /
• 2014

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.50 no.3
• /
• pp.156-160
• /
• 2005

We studied the effect of SMP (solid matrix priming) treatment, seed pretreatment for germination enhancement, to tobacco seeds by measurement of germination rate and contents of fatty acid, energy source for tobacco germination. The results were as follows. In result of germination test, germinative ability was the highest in KF109 at nine day treatment and in KB108 at seven day treatment. The composition of fatty acid in tobacco seed confirmed by gas chromatography were palmitic acid, stearic acid, oleic acid+elaidic acid, linoleic acid+linole­laidic acid, and $\alpha-linolenic$ acid. Palmitic acid, stearic acid, oleic acid and elaidic acid, and linoleic acid and linole-laidic acid were highest in KF109 at eight or nine day treatment and in KB108 at six or seven day treatment. Especially, content of oleic acid + elaidic acid, and linoleic acid+linolelaidic acid were changed largely by treatment, so these might be used for index to examine treatment effect.