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팥 포장출현력 예측을 위한 종자세 검사

Application of Seed Vigor Test for Predicting Field Emergence in Azuki Bean (Vigna angularis Wight)

  • 정관석 (경상대학교 생명과학연구원) ;
  • 나영왕 (농촌진흥청 연구정책국) ;
  • 심상인 (경상대학교 생명과학연구원) ;
  • 김석현 (경상대학교 생명과학연구원)
  • Jeong, Gwan-Seok (Research Institute of Life Science, Gyeongsang National University) ;
  • Na, Young-Wang (Research Policy Bureau, Rural Development Administration) ;
  • Shim, Sang-In (Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Seok-Hyeon (Research Institute of Life Science, Gyeongsang National University)
  • 투고 : 2014.06.14
  • 심사 : 2014.08.13
  • 발행 : 2014.09.30

초록

경실로 인해 다른 두과작물에 비해 포장 입묘가 불량한 팥의 포장출현율의 효과적인 예측 방법을 개발하기 위해 인위로 노화 정도를 조절한 종자에 대해 실험실에서 몇 가지 종자세 검사를 실시하여 포장출현력과 비교하였다. 얻어진 종자세 지표들에 대해 포장출현력과의 상관분석을 통해 예측을 위한 효율적인 지표들을 도출하였다. 팥에서 종자세가 높을 경우에는 표준발아검사에서의 종자활력률이 포장출현력 예측에 효과적이며, 약간 노화된 종자의 경우에는 저온발아검사(CGT)에서의 비정상묘율과 배축의 길이는 포장출현력과 높은 상관관계를 보였으며, 전기전도도와 CSVT에서의 활력률과 테트라졸리움 검사에서 종자세와 예측발아율은 포장출현력과 5% 수준에서 상관관계가 인정되었다. 종자세가 낮은 종자에서는 CSVT에서 불발아종자율이 포장출현력과 5% 수준에서 상관을 보였다. 포장출현력 예측에 효과적인 몇 가지 종자세지수를 이용하여 stepwise multiple regression 분석을 실시한 결과 테트라졸리움(TZ) 검사에서의 예측발아율은 결정계수($R^2$)가 0.820으로 포장출현력 예측에 가장 효과적인 검사방법임을 알 수 있었다. 표준발아검사(SGT)에서의 종자활력률, 저온발아검사(CGT)에서의 정상묘율과 건물중을 회귀방정식에 추가함에 따라 86.9% 까지 포장출현력 예측효율을 증가시킬 수 있었다.

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

키워드

참고문헌

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