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A Morphological Study on the Macrophages During Luteolysis in the Pig (돼지 황체에서 황체용해와 대식세포와의 관계)

  • 김원식;한승로;손성경;박창식;양윤석
    • Journal of Animal Science and Technology
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    • v.48 no.2
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    • pp.191-202
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    • 2006
  • In addition to the removal of dying or dead lutein cells by phagocytosis in many species, macrophages exert both luteotropic effect during maturation period and luteolytic effect during degenerative period via mediating autocrine/paracrine actions of self-producing cytokines in the corpus luteum. In this experiment, immunohistochemical and transmission electron microscopic (TEM) studies were performed to observe the morphologic changes of luteal macrophages during luteolysis. A small number of macrophages and low immunoreactivity were present at the mature stage. The number of macrophages and immunoreactivity gradually increased along the advance of luteolysis. Two subtypes of macrophages could be observed through TEM observation. One type of macrophage located between the large lutein cells contained no lipid droplets in their cytoplasm at mature stage. The other type of macrophage located near the blood vessels contained many lipid droplets in their cytoplasm during luteolysis. Particularly, no phagocytic macrophages were observed, which suggested the macrophages in the porcine corpus luteum did not involve in the phagocytotic elimination of dying lutein cells.

Development of Optimal Pruning Method on Okra (Abelmoschus esculentus L. Moench) Production (오크라 생산에 있어서 적정 적심방법 개발)

  • Ahn, Yul-Kyun;Kim, Shun-Hwan;Seong, Ki-Cheol;Moon, Doo-Kyong
    • Journal of Bio-Environment Control
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    • v.20 no.1
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    • pp.58-61
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    • 2011
  • This study was conducted to develop an optimal pruning method for okra production. Three pruning methods were tested including apical bud removing, one-third removing from the top of plant, and no pruning as a control with 3 kinds interval for 15 days after 2 months sowing. The growth and development of okra was better at the treatment of one-third removing of plant than the others. The number of branches was 0.7 in control, 3.7~4.0 in apical bud removing, and 3.0~6.0 in one-third removing treatment. In summary, one-third pruning of plant from the top of plant at 30 days after starting of pruning treatment, which showed the highest yield by 12,910 kg/10a.

Variation Operating Environment Analysis of Port Radiation Monitor (항만 방사선감시기의 검색환경 분석 연구)

  • Kim, Bo-Gyeong;Kim, Sang-Yeol;Park, Ho;Lee, Ji-Yeon
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2017.11a
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    • pp.54-55
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    • 2017
  • 본 연구는 항만에 설치된 방사선감시기의 검색기능 극대화를 위하여 방사선감시기의 검색환경을 분석하고 물류흐름 관점에서 운영환경 개선방안 도출을 목적으로 한다. 본 연구 목적 달성을 위해 원자력안전위원회에서 제시한 방사선 위험화물의 수입 물동량 분석을 수행하여 방사선감시기 설치 타당성을 확보하였다. 문헌조사와 현장 전문가 인터뷰를 통해 항만의 방사선감시기 설치환경 및 운영에 따른 물류 장애요인을 분석하였다. 또한, 항만 내 방사선감시기 설치가 가능한 위치를 3단계(본선하역작업, 야드 이송 및 적재 작업, 게이트 반출)로 구분하여 각 위치별 장 단점을 분석하고 최적의 감시기 설치 위치를 도출하였다. 연구결과, 감시기 설치 위치 단계별 비용, 물류흐름, 관리 운영적 측면을 고려하였을 때, 게이트 반출 단계가 방사선 감시기 설치를 위한 최적위치로 적합한 것으로 확인하였다. 방사선감시기의 운영최적화 방안으로는 첫째, 반출 게이트 내부의 2차 검색부지 확보를 통해 게이트 통과 전 방사선감시기를 통한 화물 검색이 이루어질 수 있도록 해야 한다. 둘째, 화물인도지시서(Delivery Order: D/O)를 활용한 신속한 화물정보 파악을 통해 물류흐름의 방해요인을 제거 할 수 있음을 알 수 있다.

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Effects of Leaf and Pod Removal on Photosynthesis and Assimilate Partition in Soybean (적엽ㆍ제협처리가 콩의 광합성과 동화물질 배분에 미치는 영향)

  • Woong Tae, Kim;Rak Chun, Seong;Harry C, Minor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.159-165
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    • 1993
  • To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.

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Study on the Vitrification of Porcine GV and M II Oocytes after Removal of Cytoplasmic Lipid Droplets (세포질 내 지방구 제거가 돼지 난포란의 유리화 동결에 미치는 영향)

  • 최인경;이승진;송해범
    • Journal of Embryo Transfer
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    • v.18 no.1
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    • pp.1-14
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    • 2003
  • This study was conducted to investigate that the immature and mature oocytes of porcine can be cryopreserved by vitrification. Oocytes were centrifuged to polarize the cytoplasmic lipid droplets. The lipids were removed from cytoplasm by micromanipulation. Delipated oocytes were centrifuged after being preincubated with cytochalasin B(CB) fer 10 min, and lipid droplets were removed. Centrifuged oocytes were treated with CB and centrifuged to polorize lipid droplets but not delipated and control oocytes is not-treatment. Oocytes of three types were vitrified in electron microscope(EM) grids. The results of survival, maturation and cleavage rates were as follows. 1 The survival rates of immature oocytes were 15.1%, 0% and 0% in the Delipated, Centrifuged and Control after vitrification, respectively, and its rate of Delipated wassignificantly higher than Centrifuged and Control(P<.01). 2. The survival rates of mature oocytes were 12.21%, 0% and 0% in the Delipated, Centrifuged and Control after vitrification, respectively, and its rate of Delipated was significantly higher than Centrifuged and Control(P<.01). 3 The maturation rates of immature oocytes were 37.5% and 68.9% for metaphase II in the Delipated after vitrification and Non-vitrification, respectively, and its rate of Non-vitrification was significantly higher than Delipated after vitrification(P<.01). 4. The cleavage rates of immature oocytes were 12.5%, 0%, 0% and 56.1% in the Delipated, Centrifuged, Control after vitrification and Non-vitrification, respectively. It's rate of Delipated was higher than Centrifuged and Control, but there were no significant difference, and its rate of Non-vitrification was significantly higher than Delipated, Centrifuged and Control(P<.05). 5 The cleavage rates of mature oocytes were 25.0%, 0%, 0% and 67.9% in the Delipated, Centrifuged, Control after vitrification and Non-vitrification, respectively. It's rate of Delipated was higher than Centrifuged and Control, but there were no significant difference, and its rate of Non-vitrification was significantly higher than Delipated, Centrifuged and Control(P<.05).

Study on Removal Time of Former Crop and Bacterial Wilt Reduction in Non-Tillage Tomato Culture by Grafting with EG-203 after Strawberry Cropping (가지대목(EG-203)을 이용한 딸기 후작 토마토 무경운 재배에서의 풋마름병 경감 및 전작물 제거시기 구명 연구)

  • Lee, Mun-Haeng;Park, Guen-Se;Lee, Hee-Keyung;Lee, Hwan-Gu;Kim, Keyng-Jae;Lee, Sun-Gye;Cho, Pyeng-Hwa;Kim, Young-Shik;Kim, Sung-Eun;Han, Kyoung Suk;Hwang, Gap-Chun
    • Korean Journal of Organic Agriculture
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    • v.22 no.4
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    • pp.657-665
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    • 2014
  • This study was carried out to study the effect of eggplant (EG203) grafting and strawberry removal time on succeeding tomato cropping in high temperature season. In non tillage culture, bacterial wilt incidence was 30% and 0% in nongrafting and eggplant (EG203) grafting while 25% in non-grafting and 0% in eggplant (EG203) grafting in tillage culture. Commercial yield was similar in tillage and non-tillage culture using EG-203 grafting as 2,693 kg/10a and 2,657 kg/10a respectively with no difference in soluble solid content and hardness. Strawberry removal time after tomato planting enhanced plant height and internode length in 20days and 30days later removal while no difference in other growth characters. Commercial yield was higher as 1,885 kg/10a in strawberry removal before tomato planting than 1,678 kg/10a in 30days later removal. From this research, strawberry removal time should be determined by considering strawberry price and growth and expecting profit by succeeding tomato cropping.

Shoot Cutting Effects on the Productivity and Nutrient Removal of Some Wetland Plants (습지식물의 지상부 제거가 생산력과 영양염류 제거량에 미치는 효과)

  • 정연숙;오현경;노찬호;황길순
    • Korean Journal of Environmental Biology
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    • v.17 no.4
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    • pp.459-465
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    • 1999
  • This work focused on the effects of the timing and the frequency of shoot cutting to maximize the productivity and the nutrient removal of three emergent macrophytes, Phragmites communis, Zizania latifolia and Typha angustifolia in natural wetlands. Shoot cutting significantly enhanced biomass production and resulted in more nitrogen and phosphorus removal from water in these three experimental species, compared to those of control. However, the frequency and the timing of shoot cutting, and the enhancement ratio were different among three species. For Phragmites stands, the highest productivity was 1.9 times of control in June treatment of the first year experiment, while 1.3 times in May treatment of the second year experiment. Zizania and Typha stands were both 1.2 times of control in August treatment and June and August treatment. Calculating the total annual removal rate of nitrogen and phosphorus based on the highest productivities among treatments, in Phragmites stands, 2.0 times of nitrogen and 1.8 times of phosphorus were removed in the first year, and both 1.4 times in the second year experiment. Likewise, for nitrogen and phosphorus; 2.4 and L.8 times in Zizania stands, and 1.8 and 1.9 times in Typha stands were removed. Overall, these results suggested that cutting treatment of shoots be effective. Thus, shoot cutting of two times during a growing season were recommended to maximize the effects: that is, in May or June, and October for Phragmites stands, and in August and October fur Zizania and Typha stands.

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Numerical Experiment on Environmental Conditions of Mass Concrete (매스콘크리트의 주변환경조건에 대한 수치실험)

  • 이장화;변근주
    • Magazine of the Korea Concrete Institute
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    • v.9 no.5
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    • pp.217-224
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    • 1997
  • 매스콘크리트의 타설시 온도이력을 정확히 예측하기 위해서는 재령별로 민감하게 변하는 시공 및 주변 환경요인을 엄밀히 반영하여 온도이력을 합리적으로 해석하는 것이 필요하다. 본 연구에서는 시공시에 발생되는 주변환경조건을 고려한 상태에서 온도이력을 해석할 수 있도록 개발된 해석프로그램을 이용하여 주변환경조건에 대한 수치실험을 수행하고자 한다. 수치실험의 목적은 매스콘크리트 타설시 각종 인자가 매스콘크리트의 온도이력에 미치는 영향정도를 정량적으로 파악하여 실제 매스콘크리트의 설계 및 시공시 사전에 콘크리트의 온도이력을 예측하여 효율적으로 관리하는데 있다. 수치실험을 수행한 결과주어진 구조물의 설계조건에 부가하여 타설시기, 타설온도,거푸집조건 및 제거시기, 양생 및 개기온도 등의 시공시 주변환경조건의 영향이 대단히 민감하게 작용하는 것으로 나타났다.

Growth and Flowering of Orostachys japonicus A. Berger as Affected by Inflorescence Pinching Time (화서제거 시기가 바위솔의 생장과 개화에 미치는 영향)

  • Jeon, Seung-Ho;Lee, Chang-Woo;Kim, Hong-Young;Shin, Sung-Cheol;Kang, Jin-Ho
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.251-255
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    • 2007
  • Floret flowering of a monocarpic plant Orostachys japonicus should be controlled to change its harvest time. The study was done to clarify the effect of pinching time of inflorescence with many florets (Aug. 25, Sept. 8 and 22, Oct. 4) on its growth, morphological characters, and flowering. Night-break treatment of 2 hours at midnight were done since Aug. 25 when afterward samples were taken every 2 weeks to Nov. 4. Growth, morphological and flowering related characters were measured from each sample. Plant height, inflorescence length and number of leaves including bracts showed significant difference between the treatments because they were sharply increased until each pinching time. Stem diameter did nearly same. Leaf and bract, floret, shoot and total dry weights had similar response to the plant height, but stem and root dry ones did similar response to the stem diameter. Most florets were removed by the pinching treatments after formed ones were increased until each pinching time. Few florets were left after the treatment, meaning that night-break treatment after that could be skipped.

Prediction of Cleaning Time of Desulfurization Absorber in a Oil-Fired Power Plant through the Operation Data (중유화력발전소의 운전자료를 이용한 탈황 흡수탑의 세정시기 예측)

  • Jang, Yeong-Gi;Lee, Jae-Heon
    • Plant Journal
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    • v.11 no.4
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    • pp.38-46
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    • 2015
  • 중유를 연소하는 D 발전소 탈황설비는 운전시간의 경과에 따라 흡수탑 중간층에 석고가 쌓이고 가스분사 파이프 내에 경질석고 스케일이 부착됨으로써 발전설비 및 탈황설비의 정상적인 운전이 어렵게 된다. 흡수탑 내부에 발생된 스케일을 제거하지 않을 경우에는 탈황효율의 저하에 따른 SO2 배출농도의 증가로 발전가능 최대출력의 하향조정이 발생되고 나아가 발전정지를 초래한다. 스케일 제거를 위한 탈황설비 세정은 발전가능 최대출력의 하향조정이 발생하는 시점으로 결정할 수 있다. 본 연구에서는 탈황설비의 운전 자료를 분석하여 발전가능 최대출력의 하향조정이 발생되기 6주 전은 탈황설비 출구 SO2 농도값이 130ppm을 초과하고 동시에 흡수탑 차압은 380mmH2O을 초과하며, 직전 흡수탑 세정 이후 44주가 경과된 시점이 됨을 확인하였다. 그리고 흡수탑의 세정시기는 세정준비기간 6주와 발전가능 최대출력 하향이 발생되기 6주 전까지의 운전경과일수 44주를 더하여 직전 세정시점으로부터 50주가 경과된 시점임을 예측하였다.

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