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Improvement of Seed Germination in Rosa rugosa (해당화의 종자 발아 연구)

  • Lee, Ji-Yeon;Lee, Ja-Hyun;Ki, Gwang-Yeon;Kim, Seung-Tae;Han, Tae-Ho
    • Horticultural Science & Technology
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    • v.29 no.4
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    • pp.352-357
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    • 2011
  • Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at $25^{\circ}C$ room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.

Occurrence of Gray Mold on Yacon Caused by Botrytis cinerea (Botrytis cinerea에 의한 야콘 잿빛곰팡이병의 발생)

  • Kim, Jeom-Soon;Lee, Young-Gyu;Kim, Su-Jeong;Hong, Sung-Kee;Choi, Hyo-Won
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.316-319
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    • 2010
  • The gray mold disease occurred on tuberous roots of yacon in storage facilities in Gangneung, Korea, in March 2010. Symptoms typically appeared as in the form of dark brown discoloration on the surface of tuberous roots and water-soaked brown lesions in cross sections of the affected portions. A total of five isolates of Botrytis sp. were obtained from the symptomatic portions. All isolates on potato-dextrose agar (PDA) produced abundant conidia which were pale brown, one-celled, mostly ellipsoid or ovoid in shape and $8.2{\sim}14.8{\times}6.5{\sim}9.9\;{\mu}m$ in size. Large numbers of round to irregular, smooth, black, hard sclerotia were produced on PDA over time. The optimal temperature for mycelial growth and sclerotia formation of the fungal isolates was $20^{\circ}C$. On the basis of morphological and cultural characteristics, all the fungal isolates were identified as Botrytis cinerea. Pathogenicity test on host plants showed that the fungus could infect not only tuberous roots but also leaves and petioles of yacon. This is the first report on gray mold of yacon (Smallanthus sonchifolius) caused by Botrytis cinerea in Korea.

Effect of Antemortem and Postmortem Environmental Temperatures on Biochemical Metabolism and Tenderness in Chicken Muscels (도계처리 전후의 환경온도가 계육의 생화학적 대사 및 연도에 미치는 영향)

  • 이유방
    • Korean Journal of Poultry Science
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    • v.6 no.1
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    • pp.24-30
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    • 1979
  • 1. The effects of heat stress (38$^{\circ}C$), cold stress (4$^{\circ}C$) and extreme cold stress (-20$^{\circ}C$) before slaughter on the tenderness and postmortem glycolysis if the excised chicken breast muscle were studied Heat stress significantly (p 0.05) increased the toughness of breast muscle. Though statistically not significant, cold stress also adversely affected the tenderness. The heat-stressed birds showed higher zero hr glycogen higher zero hr pH and significantly (p 0.05) love. ultimate pH then the controls. The cold-stressed birds showed intermediate values in these parameters. Highly significant correlations. were observed between shear value and each of these three parameters. Glycolysis rate ana final moisture content were minor factors which affected the muscle tenderness to a limited extent. The slightly elevated lactate-dehydrogenase and creatine phosphokinase activities in serum and breast muscle of stressed birds failed to account for any variations in tenderness. 2. Chicken breast and thigh muscles were subjected to different environmental temperatures to determine if the phenomenon of cold shortening exists in chicken muscle. For both breast and thigh muscles, minimum shortening was observed in the 4-10$^{\circ}C$t range. Muscles held at 0$^{\circ}C$ showed a slightly higher extent of shortening than at 4$^{\circ}C$; where as muscles held at above 20$^{\circ}C$ showed a severe shortening effect. It was concluded that no apparent cold shortening was detected in chicken muscle except at 0$^{\circ}C$ and even at 0$^{\circ}C$ and even at 0$^{\circ}C$ the extent of shortening was of a small magnitude compared to bovine muscles. Since high temperature induces a much greater shortening, muscle temperature must be lowered to below 20$^{\circ}C$ as early as possible to prevent excessive muse]e shortening.

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Induction of Apomixis by Chemical Mutagen Treatment and Ovule Development in Inbreed lines of Corn (옥수수 자식계통들에서 화학적 돌연변이 유발성질 처리에 따른 apomixis 유발과 배주발생)

  • 이호진;최근진;김태훈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.5
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    • pp.476-485
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    • 1992
  • M1 plants which were produced from seed soaking in chemical mutagen, EMS or NaN$_3$, appeared wide morphorogical variations such as dwarf, albino, twisted leaf, white streaked leaf, and purpled stem. In mutants of reproductive organs, there were monoecious plants such as female-flower plant and male-flower plant, multiple spikes, and steriled plants among M1 plants. Also, barren stalk was increased significantly in M1 plants. Ear bagging at ear initiation stage prevented seed set on cob in normal plants. In spite of ear bagging, M1 plants which had cobs with seed set was 3.9-11.2% of stalks developed from seeds soaking with mutagens, but only three or four kernels could be matured on a cob. Ear bagging after mutagen injection into initiating ear produced 5.1-10% in cobs with seed set, but only 1.7-6.3 kernels could be matured. Cobs removed silk at four hours after artificial pollination increased the rate of cobs with seed set to 27%. Microscopic observation confirmed that ontogeny of kernels matured from ear bagging and mutagen treatment would be both adventitious and diplosporous apomictic reproduction. Chromosome set of M2 seedling was found to be diploid type in chromosomal counting of root tip. As M$_2$ plants showed an uniform appearence within each lines and their CV of plant height were ranged 4-6% in each lines, we concluded that they were apomictic progeny. But we could not find any marker traits combined with apomixis.

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Quality of Dry Noodle Prepared with Wheat Flour and Immature Rubus coreanus (Bogbunja) Powder Composites (미숙복분자 분말을 첨가한 건면의 품질특성)

  • Lee, Yong-Nim;Song, Guen-Seoup;Kim, Young-Soo
    • Applied Biological Chemistry
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    • v.43 no.4
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    • pp.271-276
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    • 2000
  • Dry noodles were prepared with wheat flour and immature Rubes coreanus (Bogbunja) powder, and the effects of added immature bogbunja powders on dough rheology and noodle quality were examined. Particle size distribution of immature bogbunja powder ranged from 0.04 to $500\;{\um}m$, which was different from that of wheat flour. The initial pasting temperature, peak and final viscosities as well as setback increased in amylograph with the increase of immature bogbunja powder. The water absorption and dough development time increased, but the dough stability decreased in farinograph with the increase of immature bogbunja powder. Decrease of L and b values and increase of a value were shown with the increase of immature bogbunja powder in wheat flour-immature bogbunja powder composite as well as dry noodles. The cooked weight and volume of noodles decreased, but the cooking loss increased with the addition of immature bogbunja powder. Most of texture parameters (hardness, cohesiveness, chewiness, gumminess and tension) of cooked noodles decreased with the addition of immature bogbunja powder. From the result of sensory evaluation, dry noodles containing 1 and 2% immature bogbunja powder were rated as higher quality dry noodles than the others.

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COMPARISON OF BOND STRENGTH OF A FIBER POST CEMENTED WITH VARIOUS RESIN CEMENTS (다양한 레진시멘트로 합착한 섬유포스트의 결합강도 비교)

  • Lee, Hyun-A;Cho, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.33 no.6
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    • pp.499-506
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    • 2008
  • The purpose of this study was to compare the push-out strength of a fiber post cemented with various resin cements. Newly extracted 36 human mandibular premolars which had single root canal were selected and their crown portions were removed. The root canal was instrumented using $PROTAPER^{TM}$ system and obturated using continuous wave technique. In each root, a 9-mm deep post space was prepared. #2 translucent fiber post (DT Light post, Bisco Inc., Schaumburg, IL, U.S.A.) was cemented using injection technique with Uni-dose needle tip (Bisco) and six different resin cements. The tested resin cements were Duo-Link (Bisco Inc., Schaumburg, IL, U.S.A.), Variolink II (Ivoclar-Vivadent AG, Schann, Liechtenstein), Panavia F (Kuraray Medical Inc., Okayama, Japan), Multilink Automix (Ivoclar-Vivadent AG, Schann, Liechtenstein), RelyX Unicem (3M ESPE Dental Products, St. Paul, MN, U.S.A.), and Maxcem (Kerr Co., CA, U.S.A.). After storage in distilled water for 24 hours, each root was transversally sectioned into approximately 1-mm thick sections. This procedure resulted in 6 serial sections per root. Push-out test wasperformed using a universal testing machine (EZ Test, Shimadzu Co.) with a crosshead speed of 1 mm/min. The data were analyzed with one-way ANOVA and Tukey HSD (p=0.05). The push-out strength of the groups which cemented fiber post with Panavia F and Multilink Automix were lower than those of the other groups. But, there were no statistically significant difference among groups at a probability level of 0.05.

A Heuristic Algorithm for the Two-Dimensional Bin Packing Problem Using a Fitness Function (적합성 함수를 이용한 2차원 저장소 적재 문제의 휴리스틱 알고리즘)

  • Yon, Yong-Ho;Lee, Sun-Young;Lee, Jong-Yun
    • The KIPS Transactions:PartB
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    • v.16B no.5
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    • pp.403-410
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    • 2009
  • The two-dimensional bin packing problem(2D-BPP) has been known to be NP-hard, and it is difficult to solve the problem exactly. Many approximation methods, such as genetic algorithm, simulated annealing and tabu search etc, have been also proposed to gain better solutions. However, the existing approximation algorithms, such as branch-and-bound and tabu search, have shown the low efficiency and the long execution time due to a large of iterations. To solve these problems, we first define the fitness function to simplify and increase the utility of algorithm. The function decides whether an item is packed into a given area, and as an important information for a packing strategy, the number of subarea that can accommodate a given item is obtained from the variant of the fitness function. Then we present a heuristic algorithm BF for 2D bin packing, constructed by the fitness function and subarea. Finally, the effectiveness of the proposed algorithm will be expressed by the comparison experiments with the heuristic and the metaheuristic of the literatures. As comparing with existing heuristic algorithms and metaheuristic algorithms, it has been found that the packing rate of algorithm BP is the same as 97% as existing heuristic algorithms, FFF and FBS, or better than them. Also, it has been shown the same as 86% as tabu search algorithm or better.

Responses of Burning Liquid Propellant Sprays Perturbed by Unstable Pressure Waves (불안정한 압력파동 섭동에 의한 액체추진제 분무연소 반응)

  • Ko, Hyun;Lee, Gil-Yong;Yoon, Eung-Sub
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 1998.10a
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    • pp.3-3
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    • 1998
  • 액체추진제 로켓엔진 연소실에는 고유모드에 대응하는 음향파동이 내재되며 이러한 음향파동은 연소와의 상호작용을 통하여 불안정한 음향에너지를 공급받아 증폭되며 결국에는 연소불안정 상태에까지 이르게 된다. 이와 같은 불안정한 상태에 이르기 위해서는 연소로부터 되먹임되는 불안정 에너지의 양이 충분히 크고 구동 음향파동에 근접한 위상을 가져야 한다. 이와 같은 구동 메커니즘을 구성하는 상세한 물리적 현상들을 규명하고 예측하기 위한 많은 연구들이 보고되었으며, 이들 중 이론적인 시간 지연 모델을 사용하는 음향적인 방법은 매우 경제적인 반면 연소 현상에 대한 상세한 모사가 생략되어 연소 불안정의 구체적인 원인을 규명하는데 어려움이 있고, 파동 방정식에 의하여 연소실 내부의 파동 에너지 증가를 예측하는 방법은 연소기 내에서의 연소 메커니즘에 대한 고려 없이 연소에 의해 발생하는 에너지만을 포함하는 단점과 선형적인 연소 불안정에만 제한된다는 제한이 있다. 음향장과 커플된 기화반응 모델은 분무액적의 기화 과정이 추진제 연소의 지배과정이라는 가정 하에 연소응답을 기화반응으로 대체하는 방법으로, 역시 단시간 내에 결과를 얻을 수 있다는 장점이 있으나 기화반응으로부터 음향파동으로의 에너지 되먹임 과정이 배제되어 있어 정확한 결과를 구하기는 어렵다. 이에 대하여 최근에는 전산 모사적인 방법을 사용하는 대규모의 연소장 해석이 가능하여 짐으로써 음향파동에 의한 외란과 에너지 되먹임과정을 모두 포하마여 수치적인 방법을 사용하여 계산하는 액체추진제 로켓엔진의 고주파 연소불안정 해석방법들이 제시되고 있다.안정성 모드가 있음을 보였다. 밀도 변화가 있는 경우나 밀도 변화가 없는 경우 모두 sinuous 모드의 가장 불안정한 모드가 varicose 모드의 가장 불안정한 모드보다 더 불안정함을 보여주어 후류 유동은 자유 유동에 가까운 위상 속도를 가지는 sinuous 모드에 의해 지배될 것임을 예측할 수 있다. 연소반응이 완전연소에 가까울수록 그리고 압축성 효과가 클수록 유동내부의 온도가 증가하고 점성 또한 증가하여 후류유동은 안정됨을 알 수 있었다 유동변수들의 contour로부터 유동의 특성을 예측한 결과 baroclinic 항이 dilatational 항보다 상대적으로 크며, 중심선 상하에 생기는 vortex를 더욱 성장시킬 것으로 생각된다.냉각 홀의 막임, 연소 입자의 점착 부위 등을 예측하여 보완책을 준비할 수 있도록 하였다.$mm^2$sec였으며, 이는 다른 graphite/epixy 복합재의 확산계수와 유사한 값을 나타내고 있다. 또한 추진제가 충전된 연소관을 절단하여 밀폐한 후 95%RH 습도 조건에 보관함으로써 연소관 내부의 추진제 기계적 특성에 미치는 침투된 습기의 영향도 함께 고찰하였다. 추진제에 따라 차이는 있겠으나 추진제가 충전된 연소관은 순수 복합재 연소관에 비해 습기의 투과 정도가 작으며, 본 연소관에 충전된 RDX/AP계 추진제의 경우 추진제의 습기투과에 의한 추진제 물성 변화는 미미한 것으로 나타났다.의 향상으로, 음성개선에 효과적이라고 사료되었으며, 이 방법이 편측 성대마비 환자의 효과적인 음성개선의 치료방법의 하나로 응용될 수 있으리라 생각된다..

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Analysis of microstructure for glass-ceramics made of silicate glasses containing EAF dust (제강분진이 첨가된 규산염계 결정화유리의 미세구조 분석)

  • Kim, H.S.;Kang, S.G.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.16 no.5
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    • pp.227-234
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    • 2006
  • Microstructures of free surface and interior of glass-ceramics obtained by heat treating silicate glass specimen containing electric arc furnace dust(EAF dust) were observed. The crystallization temperature, $T_c$ of glassy specimen was measured around $850^{\circ}C$ from the result of different thermal analysis so heat treatment temperature to obtain glass-ceramic specimen was selected as $950^{\circ}C$ for 1 hr. Glass specimens containing 50 wt% dust were amorphous, while glass specimens containing 70 wt% dust showed spinel crystal peaks in XRD results. In case of glass-ceramic specimens, spinel crystalline phase was appeared with willemite, and willemite crystal peak intensity increased with increasing dust contents. The fractured surface of glass specimens containing 50 wt% dust was smooth like mirror surface, but that containing 70 wt% dust showed spinel crystals of 10 ${\mu}m$ size in glass matrix. In case of glass-ceramic specimens, ZnO crystal particles of $2{\sim}5{\mu}m$ size were produced in free surface and glassy phase, spinel and willemite crystal phases existed in interior. There were no crystals in glasses containing 50 wt% dust, while glass containing 70 wt% dust had 14 vol% crystals. Crystallinity of glass-ceramic specimens containing 50 and 70 wt% dust were 19 and 43%, respectively. When microstructures of glass and glass-ceramic specimens were observed through SEM after TCLP experiment, glass specimens showed flaking phenomenon while glass-ceramic specimens showed a slight corrosion evidence without any cracks.

Rapid detection of shiga-toxin producing E. coli by bacteriophage amplification assay (박테리오파지 증폭 기법을 활용한 시가 독소 생성 병원성 대장균의 신속 검출)

  • Baek, Da-Yun;Park, Jong-Hyun;Cho, Seok-Cheol;Lee, Young-Duck
    • Korean Journal of Food Science and Technology
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    • v.52 no.1
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    • pp.103-108
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    • 2020
  • Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacteria and can cause severe foodborne disease. For STEC detection, conventional culture methods have disadvantages in the fact that conventional culture takes a long time to detect and PCR can also detect dead bacteria. To overcome these problems, we suggest a bacteriophage amplification assay, which utilizes the ability of bacteriophages to infect living cells and their high specificity. We used a combination of six bacteriophages infecting E. coli to make the bacteriophage cocktail and added ferrous ammonium sulfate as a virucidal agent to remove free-bacteriophages. When cherry tomato and paprika were artificially inoculated with the cocktail at a final concentration of around 3 log CFU/mL and were enriched for at least 5 h in mTSB broth with Novobiocin, approximately 2-3 log PFU/mL were detected through the bacteriophage amplification assay. Therefore, bacteriophage amplification assay might be convenient and a useful method to detect STEC in a short period of time.