• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.196-202
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• 2013

This study was conducted to evaluate the performances of rice transgenic lines along with their wild types in terms of agronomic traits. A total of 32 rice transgenic lines, through previously conducted collaborative researches between molecular biologists and conventional rice breeders, were selected as promising lines. As the introduced functional genes, 17 genes, which were putatively related with high yield, disease and herbicide resistance, abiotic stress tolerance, and diversifying endosperm starch components, were transformed into three Japonica cultivars, Nipponbare, Nagdongbyeo, and Dongjinbyeo. The transgenic lines exhibited significantly deviated performances from their wild types on agronomic traits such as days to heading, culm length and yield potential. Multivariate analyses on transgenic lines to the evaluated agronomic traits also indicated random manner of phenotypic deviations from their wild type in terms of deviation directions and degrees. Our results suggested that, therefore, breeding strategies to control unexpected deleterious phenotypic performances among transgenic lines would be critical as much as the functions and proper expressions of the transformed genes.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.161-161
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• 2017

스마트 시설환경의 제어 요소는 난방기, 창 개폐, 수분/양액 밸브 개폐, 환풍기, 제습기 등 직접적으로 시설환경의 조절에 관여하는 인자와 정보 교환을 위한 통신, 사용자 인터페이스 등 간접적으로 제어에 관련된 요소들이 복합적으로 존재한다. PID 제어와 같이 하는 수학적 논리를 바탕으로 한 제어와 전문 관리자의 지식을 기반으로 한 비선형 학습 모델에 의한 제어 등이 공존할 수 있다. 이러한 다양한 요소들을 복합적으로 연동시키기 위해선 기존의 시퀀스 기반 제어 방식에는 한계가 있을 수 있다. 관행의 방식과 같이 시계열 상에서 획득한 충분한 데이터를 이용하여 제어의 양과 시점을 결정하는 방식은 예외 상황에 충분히 대처하기 어려운 단점이 있을 수 있다. 이러한 예외 상황은 자연적인 조건의 변화에 따라 불가피하게 발생하는 경우와 시스템의 오류에 기인하는 경우로 나뉠 수 있다. 본 연구에서는 실시간으로 변하는 시설환경 내의 다양한 환경요소를 실시간으로 분석하고 상응하는 제어를 수행하여 수학적이며 예측 가능한 논리에 의해 준비된 제어시스템을 보완할 방법을 연구하였다. 과거의 고성능 컴퓨팅(HPC; High Performance Computing)은 다수의 컴퓨터를 고속 네트워크로 연동하여 집적적으로 연산능력을 향상시킨 기술로 비용과 규모의 측면에서 많은 투자를 필요로 하는 첨단 고급 기술이었다. 핸드폰과 모바일 장비의 발달로 인해 소형 마이크로프로세서가 발달하여 근래 2 Ghz의 클럭 속도에 이르는 어플리케이션 프로세서(AP: Application Processor)가 등장하기도 하였다. 상대적으로 낮은 성능에도 불구하고 저전력 소모와 플랫폼의 소형화를 장점으로 한 AP를 시설환경의 실시간 제어에 응용하기 위한 방안을 연구하였다. CPU의 클럭, 메모리의 양, 코어의 수량을 다음과 같이 달리한 3가지 시스템을 비교하여 AP를 이용한 마이크로 클러스터링 기술의 성능을 비교하였다.1) 1.5 Ghz, 8 Processors, 32 Cores, 1GByte/Processor, 32Bit Linux(ARMv71). 2) 2.0 Ghz, 4 Processors, 32 Cores, 2GByte/Processor, 32Bit Linux(ARMv71). 3) 1.5 Ghz, 8 Processors, 32 Cores, 2GByte/Processor, 64Bit Linux(Arch64). 병렬 컴퓨팅을 위한 개발 라이브러리로 MPICH(www.mpich.org)와 Open-MP(www.openmp.org)를 이용하였다. 2,500,000,000에 이르는 정수 중 소수를 구하는 연산에 소요된 시간은 1)17초, 2)13초, 3)3초 이었으며, $12800{\times}12800$ 크기의 행렬에 대한 2차원 FFT 연산 소요시간은 각각 1)10초, 2)8초, 3)2초 이었다. 3번 경우는 클럭속도가 3Gh에 이르는 상용 데스크탑의 연산 속도보다 빠르다고 평가할 수 있다. 라이브러리의 따른 결과는 근사적으로 동일하였다. 선행 연구에서 획득한 3차원 계측 데이터를 1초 단위로 3차원 선형 보간법을 수행한 경우 코어의 수를 4개 이하로 한 경우 근소한 차이로 동일한 결과를 보였으나, 코어의 수를 8개 이상으로 한 경우 앞선 결과와 유사한 경향을 보였다. 현장 보급 가능성, 구축비용 및 전력 소모 등을 종합적으로 고려한 AP 활용 마이크로 클러스터링 기술을 지속적으로 연구할 것이다.

• Journal of Life Science
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• v.24 no.11
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• pp.1238-1243
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• 2014

Mesenchymal stem cells (MSCs) have been widely used as cellular therapeutic agents. They have their own characteristic stemness, and thus, they can be used in the treatment of many chronic diseases and in anticancer therapy. MSC therapy has many advantages over chemical therapy. MSC therapy is based on self or homogeneous origin; as such, it is expected to be effective in the treatment of various diseases. In addition, microRNAs in particular have been studied for their structure and function, and they are also expected to prove effective for use as therapeutic agents in cancer or chronic diseases. MicroRNAs are largely associated with metabolism and homeostasis. Therefore, over- or under-expression of microRNAs leads to chronic diseases. Conversely, effective control of the expression of specific microRNAs reduces the risk of many chronic diseases. However, there have been no reports thus far on the synergistic effects of MSCs and microRNAs. Therefore, in this study, we examined the relationship between MSCs and microRNAs using placenta-derived MSCs (PDSCs), bone marrow-derived MSCs (BM-MSCs), and fibroblast (WI-38) cells. We studied the expression of some microRNAs in MSCs and compared the expression in each cell line and cell passage. As a result, we found that the expression of microRNA-34a was higher in PDSCs than in BM-MSCs and that the expression of microRNA-27a, 33a, 33b, and 211 was higher in BM-MSCs than in PDSCs. Therefore, we expect that each MSC line will be used as cell therapy, considering its expressed functional microRNA.

• Journal of Life Science
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• v.28 no.6
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• pp.745-752
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• 2018

Melanogenesis is involved in the pigmentation of the hair, eyes, and skin in living organisms. Various signaling pathways stimulated by ${\alpha}-MSH$, SCF/c-Kit, $Wnt/{\beta}-catenin$, nitric oxide and ultraviolet activate melanocyte, leading to melanin production by tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expressed via the microphthalmia-associated transcription factor (MITF). However, the abnormal regulation of melanogenesis causes dermatological issues such as graying hair and vitiligo. Therefore, the activators that promote melanogenesis are crucial for the prevention of graying hair and the treatment of hypopigmentary disorders. Many melanogenesis stimulators have been studied for the development of novel drugs derived from synthesized compounds and natural products. Here, in addition to providing a description of a common signaling pathway in the melanogenesis of graying hair and the vitiligo process for the development of novel anti-hair graying agents, this article reviews natural herbs and the active ingredients that promote melanin synthesis as a pharmaceutical agent for the treatment of vitiligo. In particular, compounds such as Imatinib and Sugen with a stimulating effect on melanogenesis as a side effect of the drugs, are also introduced. Recent advances in research on natural plant extracts such as Polygonum multiflorum, Rhynchosia Nulubilis, Black oryzasativa, and Orysa sartiva, widely known as traditional and medicinal extracts, are also reviewed.

• Journal of fish pathology
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• v.10 no.1
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• pp.45-52
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• 1997

A protein-bound polysaccharide preparation, PS-K, isolated from Coriolus versicolor was evaluated for its ability to enhance resistance against Edwardsiella tarda, causal agent of classical edwardsiellosis in tilapia, Oreochromis niloticus. Fish treated with a dose rate of 0.1 mg PS-K $g^{-1}$ body weight were challenged by intraperitoneal injection of E. tarda with different concentrations. Against low burdens of injections($1.2{\times}10^7$ and $1.2{\times}10^8$ cfu/fish), PS-K treated fish did not show any mortality compared to 50% and 100% mortality of control groups, respectively. However there was no increase of resistance against challenge with high concentrations of E. tarda ($1.2{\times}10^9$ and $1.2{\times}10^{10}$cfu /fish) except few days delaying of death. Tilapia injected with PS-K one day after intraperitoneal inoculation of E tarda($1.2{\times}10^7$ cfu/fish) showed 100% survival rate compared to control group of 50% survival rate. The result indicates the potential of PS-K as a prophylactic agent in aquaculture. The increased antibody response in fish received PS-K one week prior to FKC administration suggested the influence of PS-K on the specific humoral immunity and increased resistance against bacterial infection.

• Korean Journal of Food Science and Technology
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• v.6 no.2
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• pp.79-85
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• 1974

To obtain further information concerning the nature myofibrillar proteins in a food system, an investigation has been conducted to compare the change in the biochemical property of the myofibril with the changes in the morphological structure of the myofibril. When myofibrils were prepared with 0.16 M KCl-0.04 M Tris-HCl, the band pattern was clear and distinct. There was a uniform thickening of A-band, a sharp appearence of Z-lines and a wide I-band. The band pattern of myofibrils was changed as the composition of extraction solution was changed. Also the ATPase activity of myofibril changed as the length of sarcomere changed. When myofibrils were treated with a low concentration of trypsin, myofibrils turned in the contracted state. With the progress of prolonged trypsin treatment, most of myofibrils exhibited a pattern of alternating light and dark bands, supercontracted pattern. Although myofibrils exhibited a supercontracted band pattern, the ATPase activity of myofibril continued to increase with the progress of trypsin treatment. An assumption was made that tropomyosin may be located in Z-line and that troponin-tropomyosin complex can inhibit the ATPase activity of myofibrils through the structural alternation of myofibril.

• Childhood Kidney Diseases
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• v.13 no.1
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• pp.84-91
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• 2009

We experienced a female neonate with congenital nephrotic syndrome (CNS) associated with congenital diaphragmatic hernia (CDH). Because of the rare combination of two conditions, we report this case with literature review. CDH was found immediately after birth and emergency operation was done for hernia repair. But on the next day, generalized edema and oliguria(0.59 mL/kg/hour) was found and her blood chemistry showed hypoalbuminemia (1.6 g/dL), increased BUN (27.7 mg/dL) and serum creatinine( 1.8 mg/dL) along with heavy proteinuria (4+). We started albumin infusion with a bolus of intravenous furosemide. We suspected the neonate had congenital nephrotic syndrome and her 24hr urine protein was 1,816 mg/day. In spite of immunosuppressive therapy, the nephrotic syndrome and renal failure progressed. We started peritoneal dialysis on the day of life 22 but it was not satisfactory. She was complicated by intracranial hemorrhage and multi-organ failure and expired at 34 days of age. Kidney necropsy was performed which showed diffuse mesangial sclerosis (DMS). Her chromosome study revealed 46, XX and her gene study revealed a heterozygous missense mutation, Arg366His, in Wilms tumor suppressor gene (WT1). This case deserves attention on account of the 4th case of CNS with CDH revealing the Arg366His mutation in the WT1 gene and G the 1st case of early onset renal failure without male pseudohermaphroditism and Wilms tumor with CNS, CDH and the Arg366His mutation in the WT1 gene. So, this report gives support to the hypothesis that Arg366His mutation in the WT1 gene can result in CNS and CDH.

• Progress in Medical Physics
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• v.11 no.1
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• pp.29-38
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• 2000

The signal transduction pathway for most neurotransmitter induced inhibition of $Ca^{2+}$ channels in sympathetic neurons involves a G-protein mediated, membrane-delimited mechanism without the participation of any known protein kinase. However, activation of protein kinase C (PKC) has been proposed as one of the intracellular mechanisms mediating some neurotransmitter induced $Ca^{2+}$ channel inhibition. In the present study, we investigated the effects of phorbol-12, 13-dibutyrate (PDBu) on $Ca^{2+}$ channel currents of acutely dispersed neurons from adult rat superior cervical ganglion (SCG) neurons using whole cell variant of the patch clamp technique. PDBu (500 nM), the activator of PKC, increased $Ca^{2+}$ channel currents and retarded the deactivation of tail currents. The effects of PDBu were voltage dependent and the maximal increase in the current amplitudes was observed between -10 to 10 mV (n=4). PDBu attenuated $Ca^{2+}$ current inhibition induced by norepinephrine (NE), which modulates $Ca^{2+}$ channels via a pertussis toxin (PTX)-sensitive pathway. Inhibition of PDBu by staurosporine (1 $\mu$M) blocked the effects of PDBu on current amplitudes and NE-induced G-protein mediated inhibition of $Ca^{2+}$ currents. Further experiment should be done to know if G-protein or $Ca^{2+}$ channel itself is the target of PKC phosphorvlation.phosphorvlation.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.117-127
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• 2012

The beneficial effects of adipose-derived stem cell conditioned media (ADSC-CM) for skin regeneration have previously been reported, despite the precise mechanism of how ADSC-CM promotes skin regeneration remaining unclear. ADSC-CM contains various secretomes and this may be a factor in it being a good resource for the treatment of skin conditions. It is also known that ADSC-CM produced in hypoxia conditions, in other words Advanced Adipose-Derived Stem cell Protein Extract (AAPE), has excellent skin regenerative properties. In this study, a human primary skin cell was devised to examine how AAPE affects human dermal fibroblast (HDF) and human keratinocyte (HK), which both play fundamental roles in skin regeneration. The promotion of collagen formation by HDFs was observed at 0.32 mg/ml of AAPE. AAPE treatment significantly stimulated stress fiber formation. DNA gene chips demonstrated that AAPE in HKs (p<0.05) affected the expression of 133 identifiable transcripts, which were associated with cell proliferation, migration, cell adhesion, and response to wounding. Twenty five identified proteins, including MMP, growth factor and cytokines such as CD54, FGF-2, GM-CSF, IL-4, IL-6, VEGF, TGF-${\beta}2$, TGF-${\beta}3$, MMP-1, MMP-10, and MMP-19, were contained in AAPE via antibody arrays. Thus, AAPE might activate the HK biological function and induce the collagen synthesis of HDF. These results demonstrate that AAPE has the potential to be used for clinic applications aimed at skin regeneration.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.243-252
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• 1985

The mechanism of reproductive cycle of longchin goby Chasmichthys dolichognathus was investigated for the annual variations of gonadosomatic index(GSI), histological changes of gonad, reproductive cycle, and initiating and terminating factors of the breeding season. The experimental fish were captured in the tide pool of Tongbaeksom, Pusan, Korea from February 1983 to September 1984. Experimental fish for the study of initiating and terminating factors of the breeding season were maintained in filtered recirculating aquariums and exposed to various temperature and photoperiod regimes. The ovary is a pair of sac-shaped organ, and the testicular structure is tubular and it is connected to the seminal vesicle which is located at the posterior end of the testis. GSI began to increase from February when the water temperature began to increase and reached peak in April. It began to decrease from August, the top water temperature season, and maintained relatively low values until January. The annual reproductive cycle includes successive six developmental stages : early growing from December to February, late growing from January to March, early mature from February to May, late mature and ripe from March to August, spawning from April to July, degeneration and resting stage from May to December. An experimental study based on the reproductive cycle of this species indicated that rising temperature under long photoperiod during spring stimulated gonadal maturation, whereas terminating factor of the breeding season with recrudescence of the gonads was demonstrated as high water temperature during summer regardless of photoperiod.