• Journal of the Korea Concrete Institute
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• v.20 no.1
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• pp.59-66
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• 2008

This study is about manufacturing and producing girder, which is an essential component of bridge structure, in a composite of FRP + concrete. This has a higher competitive power in price than steel girder. The girder used in this study is made of glass fiber which has a lower elastic modulus than steel and thus has some technical limitations such as excessive deflection compared to steel girder and lack of production facilities in FRP production companies to make a large-section component material. Thus, this study suggested a section of a new module that will allow for applying a large section in order to solve the technical difficulties mentioned above and to secure low stiffness of FRP, developed a new FRP+concrete composite girder that is filled with the appropriate amount of concrete. To identify the structural behavior of this FRP+concrete composite girder, experiments were conducted to measure its flexural strength according to the difference in the strength of confined concrete and the existence of stud. The results of the flexural strength test confirmed the composite effect from confining concrete and the effect of increase in strength proportional to the strength of concrete. In developing FRP+concrete composite girder, NDT study was also conducted to analyze the interface characteristics of concrete and FRP.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.271-279
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• 2015

Lactoferrin (LF) is a multifunctional, iron-binding glycoprotein found in physiological secretions of mammals. LF shows antibacterial, antiviral and antifungal activities. In the present study, a gene encoding the N-terminal lobe of human lactoferrin (hLF) was isolated, cloned and expressed in methylotrophic yeast, Pichia pastoris. The recombinant hLF-N (rhLF-N) protein was secreted into the culture medium at the level of $458{\mu}g/ml$ in 3 L fermentor. The size of purified hLF-N was estimated as 35 kDa when analyzed by SDS-PAGE and western blotting. The rhLF-N was further confirmed by immunodiffusion using the anti-hLF polyclonal antibody. The expression profile analysis by qRT-PCR showed that the relative mRNA expression of rhLF-N was maximal after 2-3 days of methanol induction and reduced gradually at 4 days. The purified rhLF-N showed broad antibacterial activities against the pathogens such as Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, Burkholderia cepacia, and Salmonella typhimurium. However, rhLF-N showed relatively lower activity when compared to peptides derived from LF. In spite of this weak activity, the rhLF-N expressed in P. pastoris might be more advantageous for the industrial application, because rhLF-N is secreted into the culture medium and the production can also be increased by optimization of culture conditions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1743-1749
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• 2011

The microbial reduction and quality characteristics of salted Chinese cabbage using electrolyzed water were investigated. The electrolyzed water was used to control the microbes in the processes of primary washing, salting, and secondary washing. The total bacteria, lactic acid bacteria, coliform, pH, salinity, vitamin C, and total sugar were analyzed. After primary washing by electrolyzed water, the total bacteria populations were reduced to 2.78 log cfu/g, and the coliform populations were similarly reduced. After secondary washing by electrolyzed water, the total bacteria population of Chinese cabbage was reduced to a maximum of 1.5 log cfu/g. The salinity of Chinese cabbage and salting solutions increased rapidly over three hours, and then increased slowly. The sterilization effect of electrolyzed salting water could not last beyond 3 hours, because the OHCl concentration of electrolyzed water was reduced by over 90% at the third hours of the salting process. Vitamin C was reduced and total sugar did not change regardless of treatments during the salting process. Consequently, electrolyzed water was effective to remove microbes from salted Chinese cabbages.

• Journal of the Korean Association of Geographic Information Studies
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• v.18 no.4
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• pp.131-144
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• 2015

This research proposes a prototype for visualizing radar rainfall data using the unity 3D engine. The mashup of radar data with topographic information is necessary for the 3D visualization of the radar data with high quality. However, the mashup of a huge amount of radar data and topographic data causes the overload of data processing and low quality of the visualization results. This research utilized the Unitiy 3D engine, a widely used engine in the game industry, for visualizing the 3D topographic data such as the satellite imagery/the DEM(Digital Elevation Model) and radar rainfall data. The satellite image segmentation technique and the image texture layer mashup technique are employed to construct the 3D visualization system prototype based on the topographic information. The developed protype will be applied to the disaster-prevention works by providing the radar rainfall data with the 3D visualization based on the topographic information.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.11
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• pp.44-52
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• 1998

In this paper, we proposed a node architecture and cell routing strategies for ATM applications in WDM multihop networks. The proposed node architecture employs the optical delay loop for storing the cell which is failed in out-link contention. This optical delay loop allows the delay of one cell without the electro-optic conversion. Therefore, we can get the advantages of S&F(Store-and-Forward) routing in Deflection-based all-optical networks. To support the ATM applications efficiently. we considered the transmission priority of ATM cell so that high priority cell can be transmitted with lower loss and shorter delay than low priority one. Two kinds of routing strategies are designed for this architecture: Scheme-Ⅰand Scheme-Ⅱ. Scheme-Ⅰapplies S&F routing to high cell and Deflection routing to low cell, i.e., high cells are routed along the shortest path based on S&F routing, but low cells are deflected or lost. Schem-Ⅱ is similar to Scheme-Ⅰexcept that low cells can occupy the optical loop if it is available. This Scheme-Ⅱ increases the utilization of network resources without decreasing the throughput of high cell by reducing the low cell loss rate when traffic load is low. Simulation results show that our routing strategies have better performance than conventional ones under non-uniform traffic as well as uniform traffic.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.6
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• pp.779-788
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• 2019

The compaction grouting method is one of the conventional ground improvement methods, which consolidates and compacts the surrounding ground through the injection of grout materials with low mobility. Injecting the grout into the ground can improve the soil properties, as well as form a composite of soil-grout columns. However, the conventional grout pumping is not applicable to handle multiple injection holes at the same time, which may diminish its constructability when the construction time is not enough. This paper proposes a simultaneous multiple compaction-grouting method using a new pump system developed to cover up simultaneously three injection holes at a time. Field injection tests with a single injection hole and with triangular arrangement of injection holes were conducted to evaluate the applicability of the proposed method to soft clay ground. In addition, a series of standard penetration tests (SPTs) were performed to assess the efficiency of each arrangement in improving the soft ground. It is noted from the in-situ test results that the interval distances between injection holes and the elapse time for ground stabilization are the crucial factors governing the applicability of the simultaneous multiple compaction-grouting method to improve the soft clay ground.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.7
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• pp.113-119
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• 2019

Concrete structures built on reclaimed land are combined with chemical erosion such as chlorine and sulfate ions from seawater. Chloride attack deteriorates the performance of the structure by corroding reinforcing bars. In addition, the waste water treatment structure has a problem that the concrete is deteriorated by the sulfate generated inside. Therefore, in this study, the characteristics and chemical resistance of low heat cement concrete used in wastewater treatment structures constructed on reclaimed land were evaluated. As a result of the experiment, the target slump and air content were satisfied under all the mixing conditions. The slump of low heat cement (LHC) concrete was higher than that of ordinary portland cement (OPC) concrete, while the air content of LHC concrete was smaller than that of OPC concrete with the same mix proportion. As a result of compressive strength test, OPC concrete showed higher strength at younger age compared to 28 days. In contrast, LHC concrete exhibited higher strength than OPC concrete at the age of 56 days. As a result of chlorine ion penetration tests, LHC-B concrete showed chlorine ion penetration resistance performance of the "very low" level at the age of 56 days. As a result of chemical resistance evaluation, when the LHC concrete is applied without epoxy treatment, chemical resistance is improved by about 18% compared to OPC concrete. In testing chemical resistance, the epoxy coated concrete exhibited less than 5% strength reduction when compared to sound concrete.

• Fire Science and Engineering
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• v.31 no.3
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• pp.79-87
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• 2017

Sandwich panels which are having the both sides are bonded with a heat insulating material with an iron plate are used as factories, warehouse structures as advantages of convenience in construction at economic efficiency of material cost. However, in a panel structure constructed by continuous joining of sandwich panels, a joint portion where a panel and a panel are connected is generated. The joint part is a part which is easily vulnerable to fire because flames easily flow into the melting and deformation of the iron plate during fire. The flames flowing into the panel induce diffusion of fire by rapid burning, causing damage of human life and property. In this research, we developed a flame spread prevention plate to prevent spreading of sandwich panel. This is an improvement of the workability by the anti-spreading construction method of the existing previous research, it can be applied independently to the connecting part where the panel and the panel are coupled, designed to prevent inflow and spreading of flame did. The actual fire test of the test method of KS F ISO 13784-1 of the sandwich panel specimen was conducted and the burning behavior corresponding to the presence or absence of application of the flame spread prevention plate was grasped at the panel connection part and its effect was measured. Inserting a fire spreading plate into the test result panel connecting part is measured by delaying the flashover, prevention of collapse of the specimen, and temperature rise of the opening, effectively improving the fire safety of the panel structure It was confirmed as a method that can be secured. It is judged that panel structure will contribute to ensuring fire safety by applying the fire spread prevention construction method of various methods ensuring the workability and economy of panel connection vulnerable to fire.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.83-83
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• 2017

국내에 유통되는 제초날은 대부분 금속으로 제작되며, 금속 날의 대부분 탄소공구강(SK-3~5)으로 제작되고, 날 수 및 날 형태에 따라 2도 날, 3도 날, 4도 날, 8도 날, 톱날로 구분된다. 대부분 평지 제초작업에 사용되고, 고랑 제초 작업에 알맞은 제초날의 없어 고랑 제초 작업의 효율이 떨어진다. 본 연구는 굴곡이 있는 밭의 고랑 제초 작업에 효율적인 원형 제초날을 설계 하고자 한다. 밭작물의 제초작업은 굴곡이 있는 이랑과 고랑 사이의 잡초를 제거해야 하기 때문에 평지의 잡초를 제거하는 제초날로는 제초작업이 거의 불가능하다. 따라서 고랑 형상에 맞는 원형 제초날을 설계 하였으며, 회전 균형 및 무게의 균일성 설계가 쉬운 뼈대형 원형 제초날을 설계한 후 데이터를 이용하여 원형 제초날을 설계하였다. 또한 고랑의 잡초 뿌리부분을 제거하기 위해 원형 제초날에 돌출부를 추가하는 방법으로 설계하였다. 제초날은 밭작물 고랑폭을 기준으로 하여 높이는 85mm 직경은 300mm로 설계하였으며, 제초날 설계시 원형 제초날은 뼈대형 원형 제초날과 같이 고랑폭을 기준으로 하여 높이 및 직경을 뼈대형 원형 제초날과 동일한 치수로 설계하였다. 원형 제초날 설계에서 돌출부를 볼트 결착방식으로 설계하였으며, 회전중 돌출부의 파손 혹은 이탈을 방지하기 위하여 직경 5mm이상의 볼트 결착이 필요하였다. 볼트 결착방식은 제초날 조립후 제초날 회전시 고랑의 잡초를 효과적으로 제거하기 위한 작업공간 확보가 어려울 것으로 판단되었다. 따라서 제초날 원형부에 돌출부를 절단 절곡하는 방식으로 돌출부를 재설계하였으며, 돌출부 길이는 안쪽 부분 15mm, 바깥 부분 20mm로 설계하고, 돌출부 회전반경은 안쪽 날 20mm, 바깥 날 10mm로 설계하였다. 일반적인 제초날 과 달리 원형 제초날을 개발하기 위해서 회전균형과 안전이 제초날 설계에 중요한 요인이 된다. 뼈대형 제초날을 통하여 제초날의 균형 데이터를 분석하였고 이를 바탕으로 원형 제초날을 설계하였다. 또한 발란싱 머신을 이용한 회전시험에서 unbalance 정도가 3,600rpm에서 4.51/47.1deg로 나타나 제초기에 적용 가능할 것으로 판단되었다.