• The Korean Journal of Mycology
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• v.41 no.4
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• pp.225-230
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• 2013

We investigated the cultivation characteristics of Neolentinus lepideus (21 strains derived from Korea and Japan) on Pinus densiflora sawdust medium as follows; period of mycelial growth, fruiting period, productivity of fruit body and color and morphology of fruit body. The periods for mycelial growth and fruiting on the sawdust medium of N. lepideus were 40~65 days and 6~22 days, respectively. It was produced approximately 48.3 g of fresh weight and 9 fruit-bodies per 600 g of sawdust medium. The productivity of fruit body was reduced by scratching off the surface of sawdust medium. We suggest that three strains (KFRI 1199, 1302, 1331) might be good candidates for sawdust cultivation.

• Journal of Mushroom
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• v.3 no.3
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• pp.115-118
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• 2005

This study was carried out to investigate the cultural charateristics of Fomitopsis pinicola in log woods. The optimal conditions for log cultivation as follows; 1) Light effect, natural light 2) $CO_2$, 0.2% below 3) Low temperature shock, $15^{\circ}C$ for 5 days 4) log-length, 100~120cm 5) It takes 52~65 days for primodia induction is possible to harvest fruiting bodies for 5~6 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.83-84
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• 2010

We inoculated the spawn of Inonotus obliquus on living trees of Betula platyphylla var. japonica, and could get several fruiting bodies from the inoculations. The fruiting body showed almost the same feature of that collected from natural habitats in Korea, while the size was much smaller than that of natural one. The diameter of the fruiting body ranged from 1.1 cm to 4.8 cm, and the height 0.5 cm to 2.0 cm. We expect that a harvest is possible within 5 years, but need to check the growth rate of the fruiting body to decide suitable time for getting more economic value.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.117-122
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• 2019

Myxococcus stipitatus, a myxobacterium, forms spherical fruiting bodies with stems on edaphic substrates in enrichment cultures for isolation. However, an agar medium on which purely isolated strains of M. stipitatus form this type of fruiting bodies has not been known until now. In this study, since M. stipitatus DSM 14675 forms a hemispherical fruiting body-like structure on CYS agar medium, the effects of CYS medium components on fruiting body formation were investigated. Based on the results obtained, an agar medium on which M. stipitatus forms spherical fruiting bodies with stems was developed. Additionally, a liquid medium in which M. stipitatus grows in a dispersed manner was also formulated in this investigation.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.6-10
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• 2002

The isolates of Cordyceps militaris preserved in EFCC, Kangwon National University were investigated to form the fruiting bodies under artificial conditions. The fruiting bodies were observed to be better in the 1l polyethylene bottle containing $60{\sim}80\;gm$ of brown rice and $100{\sim}110\;ml$ of water. Addition of $10{\sim}20\;gm$ of pupae per bottle showed higher fruiting. Similarly, addition of sucrose, peptone or hemoglobin also had favorable effect on fruiting. $25^{\circ}C\;and\;20^{\circ}C$ were favorable for mycelial growth and fruiting respectively. Light intensity of 500 lux and 12 h of light/dark period produced highest amount of fruit bodies.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.35-47
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• 2019

The mycelial growth of P. coccineus strain was good in PDA and YMA, but mycelial growth was low in MEA. Light irradiation during the incubation period affected the pigment formation and density of mycelia. Mushroom of P. coccineus strain was able to produce fruiting bodies in both bottle and bag cultivation, and oak sawdust was found to be the most suitable substrate for spawn culture and cultivation. In artificial cultivation using sawdust medium, fruiting body was grown to the extent that visual observation was possible from the 15th day, and it formed about 5 days fast in the treatment group with low relative humidity. From 40 to 45 days of mushroom development, mature fruiting bodies could be harvested, and the lower relative humidity of the growing room favored mushroom development and growth. Antioxidant activity of fruiting bodies harvested from artificial cultivation showed that ABTS radical scavenging activity of bottle-cultivated and wild fruit bodies were shown at 505㎍/㎖ and 515㎍/㎖, respectively. However, fruiting bodies harvested in bag cultivation were high at 910㎍/㎖. As a result of DPPH radical scavenging activity, all extracts were found to be inactive, exhibiting IC₅₀ value of more than 2,000㎍/㎖ concentration. The ethyl acetate extract of mushrooms obtained from bottle cultivation showed the highest activity with 1,550㎍/㎖ IC₅₀ value. Methanol extract of wild fruit bodies had the highest ABTS radical scavenging activity at the same concentration (10mg/㎖).

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.59-63
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• 2014

The effects of LED irradiation on growth and functional components of crops are actively being studied. We investigated the effects of red LED on the cordyceps cultivation because Cordyceps is very difficult to find in the wild and to cultivation. The synnemata formation rate of P. tenuipes was 100% under red LED. Also weight, length, and number of the synnemata were significantly increased. We expect that the red LED treatment will be of help to improve farm income.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.378-385
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• 1994

Interspecific somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus and Pleurotus sajor-caju. The fusion products between incompatible strains did not form clamp connections. Fruiting body of the clampless fusants was induced by light-dark cycle on saw-dust-rice bran substrate in glass bottles. Out of them, seven somatic hybrids produced fruiting bodies of intermediate morphology of the two species. Light and low temperature were the initiating factors for the development of clamped hyphae from the clampless mycelial colonies. All of these basidiocarps had clamp connections. Eight fusants from the six crosses were analysed with the segregation of genetic characters by random spore isolates. In the three combinations, unexpected alleles were shown. Somatic hybrid between P188 (P. ostreatus 2-1 + P. sajor-caju 2-53) and P. florida 2-3 by triple cross produced fruiting bodies similar to those of fusant between P. ostreatus and P. florida. All the genetic charaters from the three strains were shown to segregate and recombine.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.19-23
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• 2003

Temperature conditions in the mushroom cultivating room affected color, yield, pinheading rate, growth rate and other characteristics of fruitbody. These results seemed to tell the quality of mushroom. Carbon dioxide gas generated from respiration of mushroom also made stipe length long and pilei size small. High concentration of carbon dioxide could make fruitbody abnormal or dead. Mycelial shapes in fruitbody inner tissue were different according to the part and the size of fruitbody.

• Journal of Mushroom
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• v.18 no.3
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• pp.244-253
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• 2020

The aim of this study was to investigate the biological activity of extracts obtained from the mycelium, sclerotium, and fruiting body of Wolfiporia cocos using different extraction solvents (hot water, 70% ethanol, and 70% methanol). Among the three developmental stages, the mycelium extracts showed the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ferric reducing antioxidant power (FRAP), nitrite scavenging activity, and total polyphenolic content. Among the extraction solvents in the context of the W. cocos mycelium, the DPPH radical scavenging activity, FRAP, and total polyphenol content in the hot-water extracts were significantly higher than those in the other extracts. In the case of the sclerotium, the reducing power, nitrite scavenging activity, and total polyphenol content were significantly higher in 70% ethanolic extracts. The fruiting body showed the highest DPPH radical scavenging activity, reducing power, nitrite scavenging activity, and total polyphenol content in the context of hot-water extraction. Moreover, the β-glucan content was significantly higher in the sclerotium versus the mycelium or fruiting body. The total amino acid and total essential amino acid contents were remarkably higher in the mycelium and fruiting body than in the sclerotium; of note, and arginine (Arg) and phenylalanine (Phe) were highly detected among the amino acid components.