Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Formulation of a medium for the fruiting body development of Myxococcus stipitatus

Myxococcus stipitatus의 자실체 형성을 위한 배지 조성

  • Hyun, Hyesook (Department of Biotechnology, Hoseo University) ;
  • Choi, Juo (Department of Biotechnology, Hoseo University) ;
  • An, Dongju (Department of Biotechnology, Hoseo University) ;
  • Cho, Kyungyun (Department of Biotechnology, Hoseo University)
  • Received : 2019.04.01
  • Accepted : 2019.04.24
  • Published : 2019.06.30
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Abstract

Myxococcus stipitatus, a myxobacterium, forms spherical fruiting bodies with stems on edaphic substrates in enrichment cultures for isolation. However, an agar medium on which purely isolated strains of M. stipitatus form this type of fruiting bodies has not been known until now. In this study, since M. stipitatus DSM 14675 forms a hemispherical fruiting body-like structure on CYS agar medium, the effects of CYS medium components on fruiting body formation were investigated. Based on the results obtained, an agar medium on which M. stipitatus forms spherical fruiting bodies with stems was developed. Additionally, a liquid medium in which M. stipitatus grows in a dispersed manner was also formulated in this investigation.

점액세균 Myxococcus stipitatus는 순수분리를 위한 농화배양 과정에서는 자연기질 표면에서 자루가 달린 구형 자실체를 형성한다. 하지만 순수 분리된 균주가 이러한 형태의 자실체를 형성하도록 하는 한천배지는 알려져 있지 않다. M. stipitatus DSM 14675 균주는 CYS 평판배지에서 반구형 자실체 유사 구조물을 형성하므로 CYS 배지 성분이 자실체 형성에 미치는 영향을 조사하였다. 그리고 이를 기초로 M. stipitatus 세포들이 구에 자루가 달린 자실체를 형성하는 한천배지 조성을 개발하였다. 본 연구에서는 또 이와 함께 M. stipitatus DSM 14675 균주가 분산하여 성장하는 액체배지도 개발하였다.

Keywords

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Fig. 1. Growth of Myxococcus stipitatus DSM 14675 in various liquid media.

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Fig. 2. Fruiting body development of Myxococcus stipitatus DSM 14675 on various solid media.

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Fig. 3. Effects of CaCl2 and MgSO4 on the fruiting body development of Myxococcus stipitatus DSM 14675.

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Fig. 4. Effects of agar content on the fruiting body development of Myxococcus stipitatus DSM 14675.

Table 1. Dispersed growth of Myxococcus strains in various culture media

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